Article
Biotechnology & Applied Microbiology
Jingjing Hu, Ke Chen, Fangfang Hong, Guosheng Gao, Xiaoyu Dai, Hua Yin
Summary: METTL3 modifies SOCS3 mRNA with m6A to promote the stemness and tumorigenicity of LCSCs.
CANCER GENE THERAPY
(2023)
Article
Environmental Sciences
Sihao Li, Liang Pei, Qing Zhou, Zhushan Fu, Lei Zhang, Pinya Liu, Nan Yan, Shuhua Xi
Summary: Arsenic exposure increases the risk of bladder cancer by upregulating the expression of SLC1A5 and beta-catenin. SLC1A5 promotes cell proliferation and self-renewal through the activation of beta-catenin, which depends on maintaining GSH/ROS homeostasis. Therefore, SLC1A5 may be a potential therapeutic target for arsenic-induced proliferation and self-renewal of uroepithelial cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Multidisciplinary Sciences
Joachim Hanna, Flavio Beke, Louise M. O'Brien, Chrysa Kapeni, Hung-Chang Chen, Valentina Carbonaro, Alexander B. Kim, Kamal Kishore, Timon E. Adolph, Mikkel-Ole Skjoedt, Karsten Skjoedt, Marc de la Roche, Maike de la Roche
Summary: The study implicates Hedgehog signaling in Th17 polarization and intestinal immunopathology, suggesting the potential therapeutic use of Hedgehog inhibitors in the treatment of inflammatory bowel disease.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Bai Ling, Yunyang Xu, Siyuan Qian, Ze Xiang, Shihai Xuan, Jian Wu
Summary: Hematopoietic stem cells (HSCs) play a crucial role in the hematopoietic system as they can self-renew and differentiate into various blood cells. The mTOR signaling pathway is an important regulator of HSCs' differentiation, self-renewal, and quiescence, and several molecules can modulate these potentials by influencing this pathway. This review discusses the regulation of HSCs' three potentials by the mTOR signaling pathway and highlights potential regulators. Additionally, the clinical significance of studying this regulation and future predictions are also outlined.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Alejandro Ordaz-Ramos, Olivia Tellez-Jimenez, Karla Vazquez-Santillan
Summary: Breast cancer stem cells (BCSCs) are a specific subpopulation of cells capable of self-renewal and differentiation into diverse tumor cells. The involvement of cancer stem cells in treatment resistance and cancer recurrence is well established. Specific signaling pathways, such as Wnt/beta-catenin, NF-kappa B, Notch, Hedgehog, TGF-beta, and Hippo, play crucial roles in regulating the self-renewal ability of cancer stem cells. Targeting these signaling pathways holds promise as a therapeutic strategy for combating breast cancer aggressiveness. This review discusses the main signaling pathways involved in maintaining the self-renewal ability of BCSCs and highlights current strategies to disrupt stemness-related signaling molecules.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Runsheng He, Besa Xhabija, Lijin K. Gopi, Jiji T. Kurup, Zhishan Xu, Zhe Liu, Benjamin L. Kidder
Summary: The H3K4 demethylase KDM5B is overexpressed in various cancers and its high expression is associated with reduced survival. Loss of KDM5B in cancer cells leads to increased proliferation and expression of cancer stem cell markers. KDM5B also plays a role in regulating epigenetic plasticity, leading to changes in chromatin states and activation or repression of alternative transcriptional programs.
Article
Pathology
Sijie Lin, Minqing Cai, Lingyu Zhang, Yi Mao, Han Wu, Xiaodong Liu, Yixuan Li, Minghui Liang, Xinxuan Cheng, Fei Yu, Hui He, Rongrong Zong, Huping Wu, Zuguo Liu, Shangkun Ou, Wei Li
Summary: This study established a rat model and found that severe dry eye can lead to dysfunction of limbal stem cells and abnormal differentiation of corneal epithelium. The activation of the p38 mitogen-activated protein kinase signaling pathway plays a crucial role in this process.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao, Wen Cheng, Xiaoyan Xu, Juanhan Yu, Zhiguo Lin, Guoli Wang, Ling Chen, Peng Cheng, Anhua Wu
Summary: Glioblastoma (GBM) shares common signaling pathways involving TIM-3, an immune checkpoint, between tumor and non-tumor cells. The study reveals that TIM-3 in glioma cells not only regulates the malignant behaviors of glioma cells but also induces macrophage migration and transition to an anti-inflammatory/protumorigenic phenotype through the TIM-3/IL6 signal. Blocking this feedback loop may provide a novel therapeutic strategy for GBM.
Article
Cell Biology
Xuesong Feng, A. Hongjun Wang, Aster H. Juan, Kyung Dae Ko, Kan Jiang, Giulia Riparini, Veronica Ciuffoli, Aissah Kaba, Christopher Lopez, Faiza Naz, Michal Jarnik, Elizabeth Aliberti, Shenyuan Hu, Jessica Segales, Mamduh Khateb, Natalia Acevedo-Luna, Davide Randazzo, Tom H. Cheung, Pura Munoz-Canoves, Stefania Dell'Orso, Vittorio Sartorelli
Summary: Polycomb Ezh1 maintains quiescence of murine muscle stem cells (MuSCs) through a non-canonical function by regulating gene expression of the Notch signaling pathway. Loss of Ezh1 leads to premature exit from quiescence, depletion of MuSCs, and impaired muscle regeneration. Selective restoration of Notch signaling corrects stem cell number and restores quiescence in Ezh1-/- MuSCs.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Jinping Huang, Xiao Sheng, Zhangpeng Zhuo, Danqing Xiao, Kun Wu, Gang Wan, Haiyang Chen
Summary: This study identifies ClC-c as a regulator of intestinal stem cell proliferation in Drosophila, providing new insights into the functional relationship between chloride channels, stem cell maintenance, and tissue homeostasis.
CELL PROLIFERATION
(2022)
Article
Oncology
Gulab Sher, Tariq Masoodi, Kalyani Patil, Sabah Akhtar, Shilpa Kuttikrishnan, Aamir Ahmad, Shahab Uddin
Summary: Since the introduction of the cancer stem cell paradigm, significant progress has been made in understanding the role of cancer stem cells (CSCs) in tumor biology, including metastasis and drug resistance. The transcription factor FOXM1 has emerged as a key regulator of CSCs, involved in their formation, maintenance, and functionality. This review summarizes the evidence for the mechanisms by which FOXM1 promotes stemness in different types of cancers and highlights its prognostic importance.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Marine & Freshwater Biology
Ang Liang, Fan Wu, Chaojie Li, Yiyang Yu, Zimei Dong, Guangwen Chen, Fei Yu, Yanqing Yuwen, Dezeng Liu
Summary: This study investigated the toxic effect and mechanism of aspirin in planarian. The results showed that aspirin strongly inhibited stem cell proliferation and caused retarded blastemas growth. Furthermore, the expression levels of stem cell markers and target genes were significantly decreased after treatment with aspirin. The study revealed that aspirin regulates planarian regeneration through the STAT3/SOX2/OCT4 signaling pathway.
AQUATIC TOXICOLOGY
(2022)
Article
Cell Biology
Jun Yu, Qianwen Zheng, Zhiran Li, Yunhao Wu, Yangbo Fu, Xiaolong Wu, Dengfeng Lin, Cong Shen, Bo Zheng, Fei Sun
Summary: The study showed that CG6015 is essential for spermatogonia TA-divisions and elongated spermatozoon development in Drosophila melanogaster, revealing its involvement in spermatogenesis, spermatid differentiation, and metabolic processes and its relationship with the EGFR signaling pathway. Unexpectedly, a downstream target of EGFR, Dsor1, mimicked the phenotype of CG6015 with activated germline dpERK signals.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Jun Yu, Cong Shen, Meng Lin, Xia Chen, Xiuliang Dai, Zhiran Li, Yunhao Wu, Yangbo Fu, Jinxing Lv, Xiaoyan Huang, Bo Zheng, Fei Sun
Summary: In this study, we identified BMI1 as a key regulator of spermatogonial stem cell (SSC) maintenance. BMI1 represses target genes by increasing H2AK119ub and reducing H3K4me3 in SSCs. Additionally, BMI1 inhibition activates Wnt signaling and promotes nuclear translocation of beta-catenin in SSCs. Suppression of Wnt/beta-catenin signaling restores SSC maintenance in BMI1-deficient SSCs. Our findings not only reveal a novel mechanism for BMI1 in SSC maintenance, but also provide a potential strategy for treating male infertility.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
S. S. Hoepner, Ana Raykova, R. Radpour, M. A. Amrein, D. Koller, G. M. Baerlocher, C. Riether, A. F. Ochsenbein
Summary: The study demonstrates that LIGHT and its receptor LT beta R promote quiescence and self-renewal of HSCs, while LT beta R deficiency contributes to survival in a mouse leukemia model.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
George Karkoulias, Katie A. McCrink, Jennifer Maning, Celina M. Pollard, Victoria L. Desimine, Nicholas Patsouras, Miltiades Psallidopoulos, Stavros Taraviras, Anastasios Lymperopoulos, Christodoulos Flordellis
CELLULAR SIGNALLING
(2020)
Review
Medical Laboratory Technology
Clarissa A. Whitehead, Andrew H. Kaye, Katharine J. Drummond, Samuel S. Widodo, Theo Mantamadiotis, Laura J. Vella, Stanley S. Stylli
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2020)
Review
Immunology
Alexander James Sedgwick, Nazanin Ghazanfari, Patrick Constantinescu, Theo Mantamadiotis, Alexander David Barrow
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Oncology
Marija Dinevska, Natalia Gazibegovic, Andrew P. Morokoff, Andrew H. Kaye, Katharine J. Drummond, Theo Mantamadiotis, Stanley S. Stylli
Review
Oncology
Samuel S. Widodo, Ryan A. Hutchinson, Yitong Fang, Stefano Mangiola, Paul J. Neeson, Phillip K. Darcy, Alexander D. Barrow, Christopher M. Hovens, Marija Dinevska, Stanley S. Stylli, Theo Mantamadiotis
Summary: Recent developments in cancer immunotherapy offer hope for cancer patients, but difficult to treat cancers such as malignant brain cancer still present challenges, with immune cells in the tumor microenvironment playing a crucial role in regulating tumor immune suppression.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Review
Oncology
Samuel S. Widodo, Marija Dinevska, Liam M. Furst, Stanley S. Stylli, Theo Mantamadiotis
Summary: The prognosis for patients with GBM is very poor despite current standard treatments. The immunosuppressive tumour microenvironment hinders the development of effective immunotherapies for GBM, and understanding the role of IL-10 in GBM is crucial for improving immunotherapy.
BRITISH JOURNAL OF CANCER
(2021)
Review
Oncology
Michael O'Rawe, Ethan J. Kilmister, Theo Mantamadiotis, Andrew H. Kaye, Swee T. Tan, Agadha C. Wickremesekera
Summary: Glioblastoma (GB) is an aggressive brain tumor with poor prognosis despite extensive research. The renin-angiotensin system (RAS) plays a critical role in influencing cancer stem cells and tumor microenvironment in GB. Targeting RAS with existing medications presents a potential new therapeutic strategy for GB treatment.
Article
Cell Biology
Dylan Jones, Clarissa A. Whitehead, Marija Dinevska, Samuel S. Widodo, Liam M. Furst, Andrew P. Morokoff, Andrew H. Kaye, Katharine J. Drummond, Theo Mantamadiotis, Stanley S. Stylli
Summary: Glioblastoma is a highly invasive and destructive brain tumor, and current standard treatments often fail to effectively eliminate it. In this study, we investigate the effects of several FDA-approved drugs on the invasion and viability of GBM cells. We found that these drugs can reduce cell invasion and viability, suggesting their potential as therapeutic options for GBM.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Marija Dinevska, Samuel S. Widodo, Liam Furst, Lucero Cuzcano, Yitong Fang, Stefano Mangiola, Paul J. Neeson, Phillip K. Darcy, Robert G. Ramsay, Ryan Hutchinson, Fabienne MacKay, Michael Christie, Stanley S. Stylli, Theo Mantamadiotis
Summary: Tumor microenvironment in malignant astrocytoma undergoes changes in cell composition, cell signaling activation, and extracellular matrix deposition during disease progression. Targeting the extracellular matrix and cell signaling activation will be critical in designing personalized therapy.
Review
Oncology
Padmashree Rao, Liam Furst, Deborah Meyran, Chelsea Mayoh, Paul J. Neeson, Rachael Terry, Dong-Anh Khuong-Quang, Theo Mantamadiotis, Paul G. Ekert
Summary: Pediatric brain tumors are the most common solid tumor in children, and current treatments include surgery, chemotherapy, and radiotherapy. Immunotherapy, especially CAR T cell therapy, may provide new avenues for improved outcomes.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Sarah A. Cain, Bernard Pope, Stefano Mangiola, Theo Mantamadiotis, Katharine J. Drummond
Summary: This study aimed to identify somatic mutations and copy number alterations associated with grade progression in meningiomas. Through targeted next-generation sequencing, mutations and CNAs associated with different grade progression were found. NF2 mutations played an important role in grade progression.
Article
Oncology
Clarissa A. Whitehead, Haoyun Fang, Huaqi Su, Andrew P. Morokoff, Andrew H. Kaye, Eric Hanssen, Cameron J. Nowell, Katharine J. Drummond, David W. Greening, Laura J. Vella, Theo Mantamadiotis, Stanley S. Stylli
Summary: In this study, it was found that glioblastoma cells promote invadopodia activity by secreting small extracellular vesicles (sEVs) containing matrix metalloproteinase MMP-2. Additionally, the invasiveness of cells and sEV secretion were increased after radiotherapy/temozolomide treatment. These findings reveal a relationship between sEVs and invadopodia, providing important insights into the functional capacity of sEVs in promoting glioblastoma cell invasion.
Article
Developmental Biology
Tom C. Karagiannis, Christian Orlowski, Katherine Ververis, Eleni Pitsillou, Gulcan Sarila, Samuel T. Keating, Laura J. Foong, Stefanie Fabris, Christina Ngo-Nguyen, Neha Malik, Jun Okabe, Andrew Hung, Theo Mantamadiotis, Assam El-Osta
Summary: This study investigates the role of phosphorylated histone H2AX (gamma H2AX) as a marker of DNA double-strand breaks (DSBs) and its implication in stem cell differentiation. The research establishes a mouse embryonic stem cell (mESC) differentiation model and examines the dynamics of gamma H2AX foci during the process. The findings reveal that gamma H2AX serves as a versatile marker of DSBs and may have a role as a biomarker in stem cell differentiation.
CELLS & DEVELOPMENT
(2024)
Review
Biochemistry & Molecular Biology
Simon Haron, Ethan J. Kilmister, Paul F. Davis, Stanley S. Stylli, Theo Mantamadiotis, Andrew H. Kaye, Sean R. Hall, Swee T. Tan, Agadha C. Wickremesekera
Summary: The Renin-Angiotensin System (RAS) plays a crucial role in both CNS tumors and degenerative diseases by regulating various signaling pathways that affect cellular functions and the development of diseases.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
Article
Biochemical Research Methods
Maria Mougkogianni, Argyro Kalogeropoulou, Nickolaos Nikiforos Giakoumakis, Zoi Lygerou, Stavros Taraviras
Summary: Recruitment of the MCM complex on DNA replication origins is critical for faithful genome duplication, with aberrant licensing linked to cancer and neurodevelopmental diseases. An optimized approach for qualitative and quantitative analysis of DNA-bound MCMs in the developing mouse cortex has been developed, offering innovative insights into in vivo research on origin licensing.
