Article
Chemistry, Multidisciplinary
Mingle Li, Kalayou Hiluf Gebremedhin, Dandan Ma, Zhongji Pu, Tao Xiong, Yunjie Xu, Jong Seung Kim, Xiaojun Peng
Summary: The article introduces a conditionally activatable photoredox catalysis method, which achieves more effective tumor treatment through site-specific photoredox. This approach enables control of photoresponsivity while ensuring biocompatibility and safety.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Gao Wang, Trung Phan, Shengkai Li, Jing Wang, Yan Peng, Guo Chen, Junle Qu, Daniel Goldman, Simon A. Levin, Kenneth Pienta, Sarah Amend, Robert H. Austin, Liyu Liu
Summary: Experimental robobiological physics explores the impact of genetic diversity and different landscapes on the survival of autonomous robots, shedding light on biological evolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Qi-Chao Yang, Shuo Wang, Yuan-Tong Liu, An Song, Zhi-Zhong Wu, Shu-Cheng Wan, Hui-Min Li, Zhi-Jun Sun
Summary: PCSK9 expression is upregulated in HNSCC tissues and higher expression is associated with poorer prognosis. Inhibition of PCSK9 suppresses cancer cell stemness via LDLR-dependent mechanism. PCSK9 inhibition enhances CD8(+) T cell infiltration, reduces MDSCs, and improves the efficacy of anti-PD-1 ICB therapy in HNSCC.
Review
Biochemistry & Molecular Biology
Dong-Oh Moon
Summary: This review provides a comprehensive analysis of the multifaceted role of calcium in apoptosis regulation, focusing on its associated signaling pathways and molecular mechanisms. It explores the impact of calcium on apoptosis through its effects on various cellular compartments and highlights its connection to ER stress. Additionally, it discusses the interplay between calcium and proteins such as calpains, calmodulin, and Bcl-2 family members, and its role in regulating caspase activation and pro-apoptotic factor release. Understanding the complex relationship between calcium and apoptosis is crucial for identifying potential treatment options for diseases associated with imbalanced cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Ruben Faria, Prisca Boisguerin, Angela Sousa, Diana Costa
Summary: Mitochondria are crucial cellular organelles responsible for energy production in cells, and mutations in their DNA (mtDNA) can lead to various diseases. Mitochondrial gene therapy is a promising strategy to address these disorders. Developing efficient mtDNA-based delivery systems to target and transfect mammalian mitochondria is an exciting area of research that can contribute to restoring normal mitochondrial function.
Article
Biochemistry & Molecular Biology
Alex Gallinat, Gemma Vilahur, Teresa Padro, Lina Badimon
Summary: Infarct size is a major risk predictor for heart failure after acute myocardial infarction (AMI). The discovery of conditioning phenomena has revealed the existence of endogenous protective mechanisms that can limit infarct size after revascularization. However, most strategies aiming to reduce infarct size have failed in clinical studies, highlighting the need for cardioprotection. This study used a network-assisted systems biology approach to explore the mitochondrial proteomic signature of the myocardium in a swine model of AMI. The results identified cardiac metabolism as a driver of cardioprotection and highlighted the role of post-conditioning in promoting metabolic reprogramming of the myocardium and a protective response mediated by VDAC2 and DJ-1 in the mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Wojciech Trybus, Teodora Krol, Ewa Trybus, Anna Stachurska
Summary: Physcion exerts antitumor effects by inducing oxidative stress, autophagy, and apoptosis in HeLa cells, resulting in decreased cell viability, G0/G1 phase cell cycle arrest, and activation of the lysosomal system.
Review
Medicine, General & Internal
Isa Mambetsariev, Jeremy Fricke, Stephen B. Gruber, Tingting Tan, Razmig Babikian, Pauline Kim, Priya Vishnubhotla, Jianjun Chen, Prakash Kulkarni, Ravi Salgia
Summary: In recent decades, the advancement of high-throughput approaches in cancer biology and medicine has led to the development of targeted therapies and immunotherapies for different cancers. The availability of diverse high-throughput omics data has revealed the complex nature of cancer as a system of microenvironments, sub-clonal tumor populations, and various cell types, impacting the genetic and non-genetic mechanisms involved in the disease. Taking a systems approach to cancer biology has been essential in understanding tumor initiation, progression, and drug resistance. Integrating clinical medicine with basic sciences has revolutionized the development and approval of cancer therapeutic drugs including tyrosine kinase inhibitors, antibody-drug conjugates, and immunotherapy. By further improving the 'Team Medicine' approach within the cancer systems biology framework, through the development of high-throughput clinical trial models utilizing machine learning and rapid sample processing, personalized medicine can advance more rapidly, overcoming limitations in drug development and testing.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemical Research Methods
Shan-Ju Yeh, Bor-Sen Chen
Summary: This study proposes a systematic approach to investigate the molecular progression mechanisms of gastric cancer and identifies critical biomarkers and corresponding drugs. By utilizing big database mining, system modeling, and deep learning techniques, the study successfully reveals the progression of gastric cancer and suggests potential multi-target drugs.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2022)
Article
Oncology
Oxana V. Denisova, Joni Merisaari, Riikka Huhtaniemi, Xi Qiao, Laxman Yetukuri, Mikael Jumppanen, Amanpreet Kaur, Mirva Paakkonen, Carina von Schantz-Fant, Michael Ohlmeyer, Krister Wennerberg, Otto Kauko, Raphael Koch, Tero Aittokallio, Mikko Taipale, Jukka Westermarck
Summary: Mitochondrial glycolysis and hyperactivity of the AKT pathway are characteristic of brain tumors, but targeting AKT or PDK has shown limited clinical benefits. In this study, a triplet therapy targeting AKT, PDK, and PP2A was effective in inducing apoptosis in brain tumor cells. Reactivating PP2A converted the cytostatic response to cytotoxic apoptosis by shutting down compensatory mitochondrial oxidative phosphorylation. These findings suggest the potential of targeting mitochondrial metabolism to overcome therapy tolerance in brain tumors.
MOLECULAR ONCOLOGY
(2023)
Review
Cell Biology
Yibo Fu, Francesca Ricciardiello, Gang Yang, Jiangdong Qiu, Hua Huang, Jianchun Xiao, Zhe Cao, Fangyu Zhao, Yueze Liu, Wenhao Luo, Guangyu Chen, Lei You, Ferdinando Chiaradonna, Lianfang Zheng, Taiping Zhang
Summary: Mitochondria play important roles in the chemoresistance of pancreatic cancer by affecting apoptosis, metabolism, mtDNA metabolism, and mitochondrial dynamics. Targeting certain factors in mitochondria may improve the sensitivity of pancreatic cancer cells to chemotherapeutic agents.
Article
Chemistry, Multidisciplinary
Meng Yuan, Shuang Liang, Ying Zhou, Xiao Xiao, Bin Liu, Chunzheng Yang, Ping'an Ma, Ziyong Cheng, Jun Lin
Summary: The study demonstrates that hemoglobin (Hb) as a sonosensitizer shows great potential and has better sensitization efficiency compared to traditional sonosensitizers, effectively avoiding the low ROS quantum yield problem. By integrating with ZIF-8 to form a nanoplatform, it can effectively suppress deep-seated tumors under ultrasound irradiation.
Article
Cell Biology
Taeyeon Hong, Jiyeon Ham, Jisoo Song, Gwonhwa Song, Whasun Lim
Summary: Brassinin exerts antiproliferative effects on HCC cells by reducing PCNA activity and inducing cell cycle arrest, increasing mitochondrial Ca2+ levels, generating ROS, and regulating AKT and MAPK signaling pathways. Co-administration with pharmacological inhibitors enhances its antiproliferative effects.
Review
Biochemistry & Molecular Biology
Peticha Tanprasert, Sirikan Limpakan (Yamada), Siriporn C. Chattipakorn, Nipon Chattipakorn, Krekwit Shinlapawittayatorn
Summary: This review discusses the homeostasis of gastric cancer cells and highlights the potential role of mitochondria in gastric cancer therapy.
Review
Biochemistry & Molecular Biology
Michela Peruch, Emiliana Giacomello, Davide Radaelli, Monica Concato, Riccardo Addobbati, Alessandra Lucia Fluca, Aneta Aleksova, Stefano D'Errico
Summary: Cocaine abuse leads to complex cardiotoxicity, involving multiple mechanisms such as sodium channel blockade, increased oxygen demand, mitochondrial dysfunction, and promotion of apoptosis. Research on mitochondrial mechanisms has mainly been conducted in animal models, with limited studies in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Donald Wlodkowic, Olivia Campana
Summary: The fish embryo toxicity test (FET) has gained popularity as a sensitive alternative in chemical risk assessment and water quality evaluation, but the actual manipulation remains labor intensive. The advances in fluidics and laboratory automation systems offer promising opportunities for high-throughput FET testing, while facing existing challenges in toxicity assessment.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Environmental Sciences
Yutao Bai, Jason Henry, Olivia Campana, Donald Wlodkowic
Summary: Neurotoxicity effects of industrial contaminants are currently not well studied, with behavioral changes in small aquatic invertebrates being proposed as useful indicators, but limited by lack of advancements in analytical technologies. Advances in Lab-on-a-Chip technologies show promise in addressing these limitations and facilitating high-throughput neuro-behavioral ecotoxicity testing.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Genetics & Heredity
Alina Kurolap, Florian Kreuder, Claudia Gonzaga-Jauregui, Morasha Plesser Duvdevani, Tamar Harel, Luna Tammer, Baozhong Xin, Somayeh Bakhtiari, James Rice, Clare L. van Eyk, Jozef Gecz, Jean K. Mah, Derek Atkinson, Heidi Cope, Jennifer A. Sullivan, Alon M. Douek, Daniel Colquhoun, Jason Henry, Donald Wlodkowic, Yesim Parman, Ayse Candayan, Elif Kocasoy-Orhan, Anat Ilivitzki, Shiri Soudry, Rina Leibu, Fabian Glaser, Valerie Sency, Gil Ast, Vandana Shashi, Michael C. Fahey, Esra Battalog, Albena Jordanova, Vardiella Meiner, A. Micheil Innes, Heng Wang, Orly Elpeleg, Michael C. Kruer, Jan Kaslin, Hagit Baris Feldman
Summary: Cell adhesion molecules play a crucial role in nervous system development and function. This study identifies NRCAM variants associated with a neurodevelopmental disorder and provides evidence of the detrimental effects on NRCAM structure and function. The findings from in vitro and in vivo experiments further support the importance of NRCAM in the nervous system.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Review
Biochemical Research Methods
Donald Wlodkowic, Aleksandra Czerw, Beata Karakiewicz, Andrzej Deptala
Summary: Environmental toxicology focuses on identifying and predicting the impact of potentially toxic chemicals on the biosphere, with an increased emphasis on understanding cellular and sub-cellular mechanisms of ecotoxicity. Advanced cytometry technologies offer powerful capabilities for analyzing cells, tissues, and small model organisms, overcoming the limitations of traditional techniques and providing new insights into ecotoxicology research.
Article
Engineering, Environmental
Danielle L. Tomasello, Donald Wlodkowic
Summary: The significance of neurotoxicological risks associated with anthropogenic pollution is being recognized worldwide. However, there is still a lack of understanding regarding the neurophysiological foundations responsible for the altered behavioral phenotypes. Noninvasive, in situ electrophysiology based on multielectrode arrays (MEAs) offers a promising approach for studying neurobehavioral changes in aquatic species.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Editorial Material
Pharmacology & Pharmacy
Donald Wlodkowic
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Veterinary Sciences
Jason Henry, Yutao Bai, Daniel Williams, Adrian Logozzo, Alex Ford, Donald Wlodkowic
Summary: In this article, the authors investigate how the geometry, size, opacity, and depth of test chambers affect the behavioral responses in Artemia franciscana. They find that these factors can modulate the spontaneous behavioral responses of the larval stages of A. franciscana. The authors also observe that A. franciscana exhibits preferences for color and depth, and these preferences can be altered with photic stimulus.
Article
Chemistry, Analytical
Donald Wlodkowic, Tomasz M. Karpinski
Summary: Continuous monitoring and early warning of potential water contamination with toxic chemicals is crucial for human health and sustainable food production. While there have been advances in automated sensing of water parameters, the online monitoring of chemical pollutants remains a challenge. Biological early warning systems using live-cell biosensors offer promising avenues for detecting contaminants and supplementing traditional water quality testing strategies. Further technological innovations and deployment successes are needed to fully realize the potential of live-cell biosensors in water quality monitoring.
Article
Developmental Biology
Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic
Summary: This study demonstrates the advantages of using custom embryo arrays and an ultra-high-definition video imaging system for large-scale chemobehavioral phenotyping with zebrafish embryos. It also presents a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of embryos.
Article
Environmental Sciences
Jason Henry, Jack A. Brand, Yutao Bai, Jake M. Martin, Bob B. M. Wong, Donald Wlodkowic
Summary: Contamination by pharmaceutical pollutants poses a critical threat to aquatic ecosystems. A study found that long-term exposure to the antidepressant fluoxetine reduces behavioral plasticity and egg mass production in freshwater snails, highlighting the potential harmful effects on non-target organisms and population resilience.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Jason Henry, Yutao Bai, Florian Kreuder, Minna Saaristo, Jan Kaslin, Donald Wlodkowic
Summary: This study demonstrates the application of a high-throughput multidimensional behavioral test battery in larval zebrafish and reveals the effects of certain drugs on their behaviors. The results highlight the significance of high-throughput multidimensional behavioral ecotoxicity test batteries in assessing the risks of neuroactive drugs and screening emerging contaminants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Yutao Bai, Jason Henry, Tomasz M. Karpinski, Donald Wlodkowic
Summary: Analysis of sensorimotor behavioral responses to stimuli such as light can provide enhanced relevance in prioritizing chemical risks. However, there have been minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testing due to technical limitations. In this research, a high-throughput phototactic biotest system for brine shrimp nauplii was demonstrated, along with a novel bioinformatic approach for analyzing phototactic behaviors at scale. The preliminary data showed consistent light-seeking behaviors in nauplii without habituation, and exposure to sub-lethal concentrations of insecticides resulted in perturbed light seeking behaviors. These findings suggest that phototactic bioassays have the potential to be a sensitive, responsive, and cost-effective method in behavioral ecotoxicology.
Article
Behavioral Sciences
Jack A. Brand, Jason Henry, Gabriela C. Melo, Donald Wlodkowic, Bob B. M. Wong, Jake M. Martin
Summary: Recent research suggests that individuals vary in the consistency of their behavior, and that these differences may be heritable. This study investigated whether there are sex differences in the predictability of risk-taking behavior in male and female eastern mosquitofish. The results showed consistent sex differences in mean-level risk-taking behavior, but the magnitude and direction of sex differences in behavioral predictability were assay-specific.
BEHAVIORAL ECOLOGY
(2023)
Article
Engineering, Environmental
Yutao Bai, Jason Henry, Eva Cheng, Stuart Perry, David Mawdsley, Bob B. M. Wong, Jan Kaslin, Donald Wlodkowic
Summary: Aquatic eco-neurotoxicology is a new field that focuses on studying the effects of pollutants on animal behaviors, particularly the cognitive behaviors affected by neurotoxicants. However, the scarcity of experimental data is due to the lack of low-cost technologies for analyzing higher-level neurological functions in small aquatic organisms. In this study, a proof-of-concept prototype was presented, utilizing real-time animal tracking software and closed-loop external hardware for delivering stimuli based on specific behaviors. The system demonstrated precise animal tracking across different aquatic species and has potential applications in cognitive research of small aquatic model organisms.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Medicine, Research & Experimental
Tomasz M. Karpinski, Marcin Ozarowski, Agnieszka Seremak-Mrozikiewicz, Hubert Wolski, Donald Wlodkowic
Summary: The global outbreak of COVID-19 has emphasized the urgent need for effective vaccines and treatments. Advances in biotechnology and genetic engineering have provided progress in understanding the virus, with the development of vaccines being a key focus. Challenges in vaccine development and distribution, as well as the importance of safety and long-term effectiveness, have also been highlighted.