Article
Pharmacology & Pharmacy
Zhenghan Dong, Yachao Wang, Cuiting Hao, Yuan Cheng, Xi Guo, Yanyu He, Yueyue Shi, Shuang Wang, Yunqi Li, Wei Shi
Summary: This study found that the extract of S. Sanghuang (SSE) has positive effects on nematode indicators. Different concentrations of SSE can prolong the lifespan of nematodes and increase it by 26.41%. SSE also reduces the accumulation of endogenous aging substances, increases antioxidant capacity and enhances physical fitness. RT-PCR analysis shows that SSE treatment upregulates the expression of a series of genes, prolonging the lifespan of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress, providing a theoretical basis for its application in anti-aging treatments.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Food Science & Technology
Jiaqian Hong, Yiming Song, Jiayan Xie, Jianhua Xie, Yi Chen, Ping Li, Danyang Liu, Xiaobo Hu, Qiang Yu
Summary: This study used Caenorhabditis elegans as a model organism to investigate the toxic effects and mechanisms of acrolein (ACR) exposure. The results showed that ACR exposure shortened the lifespan of C. elegans, reduced reproductive capacity and locomotive behavior, and increased oxidative stress levels. ACR also affected the expression of antioxidant-related genes. The findings suggest that ACR exposure induces aging and oxidative stress in C. elegans, activating the DAF-16/FOXO pathway.
Article
Medicine, Research & Experimental
Xingzhuo Song, Yu Du, Cen Liu, Wei Wang, Jing Han, Xinlou Chai, Yonggang Liu
Summary: Oxidative stress disrupts redox state homeostasis and induces apoptosis. Prolonged oxidative stress can impair cell, tissue, and organ function, leading to the development of diseases. H-2 synthesized from N-Alkylamides (NAAs) of Anacyclus pyrethrum (L.) DC was investigated for its antioxidant activity and molecular mechanisms using Caenorhabditis elegans (C. elegans) and B16-F10 mouse melanoma cell models. In vivo studies on C57BL/6 mice showed the anti-vitiligo activity of H-2. The results demonstrated that H-2 increased the survival time of nematodes under oxidative stress, promoted the nuclear localization of DAF-16, and enhanced the expression of Superoxide Dismutase 3 (SOD-3) in nematodes, thus activating the antioxidant enzyme system. H-2 also reversed oxidative stress damage in the B16-F10 cell model and increased the number of melanocytes in vitiligo model mice, suggesting its potential in vitiligo treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Rebecca L. McIntyre, Simone W. Denis, Rashmi Kamble, Marte Molenaars, Michael Petr, Bauke Schomakers, Mizanur Rahman, Siddhartha Gupta, Marton L. Toth, Siva A. Vanapalli, Aldo Jongejan, Morten Scheibye-Knudsen, Riekelt H. Houtkooper, Georges E. Janssens
Summary: Transcriptome-based drug screening is a powerful tool for identifying geroprotective compounds targeting age-related disease. The study identified FDA-approved compounds, such as atracurium, that activate FOXO downstream targets and extend lifespan in Caenorhabditis elegans. It highlights the role of the neuromuscular acetylcholine receptor in regulating the highly conserved FOXO/DAF-16 longevity pathway.
Article
Environmental Sciences
Chi-Wei Huang, Wan-Ru Liao, Chun Ming How, Pei-Ling Yen, Chia-Cheng Wei
Summary: The study found that ZEN significantly decreased locomotive behaviors in C. elegans. Lifespan and aging markers were also adversely affected by ZEN, which increased ROS levels and downregulated antioxidant genes. Long-term exposure to ZEN induced aging-related decline in C. elegans, associated with oxidative stress and inhibition of antioxidant defense.
ENVIRONMENTAL POLLUTION
(2021)
Article
Pharmacology & Pharmacy
Lin Tan, Zhuo-Ya Zheng, Lv Huang, Zhong Jin, Su-Lian Li, Gui-Sheng Wu, Huai-Rong Luo
Summary: This study found that Complanatoside A (CA) could extend the lifespan of Caenorhabditis elegans and improve their physiological functions. CA also reduced the accumulation of toxic proteins and delayed the onset of neurodegenerative disorders. The study suggested that CA is a potential antiaging agent worth further research.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Huihui Ji, Zhimin Qi, Daniel Schrapel, Monika Le, Yiqiao Luo, Bin Yan, Jury Gladkich, Michael Schaefer, Li Liu, Ingrid Herr
Summary: Sulforaphane can prolong lifespan and healthspan by inducing the expression and nuclear translocation of TRA-1/GLI, and thus support healthy aging by regulating daf-16/FOXO. However, the prolonging effects of sulforaphane on lifespan and healthspan are inhibited when TRA-1 or daf-16 is interfered with by RNA interference or using worms with mutants of tra-1 or daf-16 genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Wenqi Wang, Xin Feng, Yu Du, Cen Liu, Xinxin Pang, Kunxiu Jiang, Xirui Wang, Yonggang Liu
Summary: By modifying pinocembrin with amino acids, the derivative pb-3 showed promising antiaging effects in increasing tolerance to thermal and oxidative stress, as well as reducing lipofuscin accumulation in Caenorhabditis elegans. The antioxidant and antiaging properties of pb-3 may involve the DAF-16/FOXO transcription process. These findings suggest that pinocembrin amino acid derivatives could be a novel agent for antiaging therapy.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Hui Wang, Shuai Zhang, Lu Zhai, Liwei Sun, Daqing Zhao, Zeyu Wang, Xiangyan Li
Summary: The study showed that total ginsenosides (TGS) can extend the lifespan of worms, improve mobility, have antioxidant and health benefits, and achieve these effects by activating signaling pathways related to antioxidant regulation and longevity.
Review
Cell Biology
Mohankumar Amirthalingam, Sundararaj Palanisamy, Shinkichi Tawata
Summary: PAKs are a group of kinases involved in a wide range of cellular functions and have been implicated as not only oncogenic but also aging kinases. Targeting PAKs could be a potential strategy for cancer therapy development with minimal side effects and promoting healthy longevity.
AGEING RESEARCH REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Xiang Xiao, Yurong Zhou, Cui Tan, Juan Bai, Ying Zhu, Jiayan Zhang, Xinghua Zhou, Yansheng Zhao
Summary: Barley beta-glucan shows potential in defending oxidative stress in C. elegans by reducing ROS levels, enhancing SOD and CAT enzyme activity, decreasing MDA content, and extending lifespan. It participates in anti-oxidative stress partially through the daf-2/daf-16 pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Maria Alejandra Guerrero-Rubio, Samanta Hernandez-Garcia, Francisco Garcia-Carmona, Fernando Gandia-Herrero
Summary: Flavonoids, potential nutraceutical compounds found in dairy food, are considered health-promoting compounds with promising therapeutic effects on neurological, inflammatory diseases, diabetes, and cancer. In Caenorhabditis elegans, chrysin, 6-hydroxyflavone, and baicalein were found to significantly extend lifespan, with effects mediated by different signaling pathways. Microarray analysis showed that baicalein downregulates important age-related genes such as mTOR and PARP.
Article
Biochemistry & Molecular Biology
Meng-Lu Sun, Xin-Yan Chen, Jin-Jin Cao, Xiang-Huan Cui, Hong-Bing Wang
Summary: The study showed that Polygonum multiflorum Thunb extract can extend lifespan, improve mitochondrial function, enhance antioxidant capacity in Caenorhabditis elegans, possibly through the mediation of certain transcription factors.
Article
Chemistry, Medicinal
Jing-Shan Wu, Chun-Guo Lin, Chang-Long Jin, Yan-Xia Zhou, Ying-Xiu Li
Summary: The marine bacterium Planococcus maritimu ML1206 was found to have antioxidant properties and could enhance the ability of nematodes to resist oxidative stress and extend their lifespan. These findings suggest that ML1206 has potential anti-aging and antioxidant functions and could be used in aquaculture and as a dietary supplement.
Article
Nutrition & Dietetics
Guillaume Cardin, Cyril Poupet, Muriel Bonnet, Philippe Veisseire, Isabelle Ripoche, Pierre Chalard, Anne Chauder, Etienne Saunier, Julien Priam, Stephanie Bornes, Laurent Rios
Summary: The relationship between food and health plays a crucial role in disease prevention and life expectancy, with fermented foods offering microbial biodiversity that can potentially provide health benefits. Research has shown that raw goat milk cheese and its extracts can increase the longevity of nematodes by activating specific pathways, while also potentially enhancing oxidative-stress resistance. Further investigation is necessary to fully understand the biological mechanisms involved.
Article
Medicine, General & Internal
Roberta Cascella, Martina Banchelli, Seyyed Abolghasem Ghadami, Diletta Ami, Maria Cristina Gagliani, Alessandra Bigi, Tommaso Staderini, Davide Tampellini, Katia Cortese, Cristina Cecchi, Antonino Natalello, Hadi Adibi, Paolo Matteini, Fabrizio Chiti
Summary: This study investigated the morphological, structural, and tinctorial properties of TAR DNA-binding protein 43 (TDP-43) inclusions in neuroblastoma x spinal cord 34 (NSC-34) cells. The results showed that TDP-43 inclusions did not exhibit amyloid properties and lacked cross-beta structure and fibrillar morphology. These findings suggest that TDP-43 has a low propensity to form amyloid fibrils and instead forms other types of inclusions.
ANNALS OF MEDICINE
(2023)
Article
Environmental Sciences
Giulia Guarnieri, Matteo Becatti, Roberta Squecco, Paolo Comeglio, Rachele Garella, Lara Tamburrino, Sara Marchiani, Linda Vignozzi, Gabriella Barbara Vannelli, Mario Maggi, Annamaria Morelli
Summary: The neuroendocrine control of reproduction is regulated by the pulsatile release of GnRH by hypothalamic neurons. BaP, a pollutant, can disrupt endocrine function and affect gonadal function and gamete maturation. This study found that BaP alters the phenotype and function of GnRH neurons by interfering with KISS1R signaling and GnRH secretion, highlighting its importance in the central neuroendocrine control of reproduction.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Damiano Cirri, Andrea Geri, Lara Massai, Michele Mannelli, Tania Gamberi, Francesca Magherini, Matteo Becatti, Chiara Gabbiani, Alessandro Pratesi, Luigi Messori
Summary: A panel of four novel gold(I) complexes, inspired by the clinically established gold drug auranofin, were prepared and characterized. The differences in their interactions with model proteins were analyzed, and their cytotoxic effects on ovarian cancer cells were quantitated. These novel gold complexes showed potential as effective anticancer agents.
Article
Biochemistry & Molecular Biology
Michela Cirillo, Flavia Rita Argento, Monica Attanasio, Matteo Becatti, Irene Ladisa, Claudia Fiorillo, Maria Elisabetta Coccia, Cinzia Fatini
Summary: This study aimed to explore cardiometabolic markers and oxidative stress and evaluate the effects of the Mediterranean Diet (MD) in modulating these markers. The results showed that after a 3-month intervention with MD, there was a significant reduction in total cholesterol and LDL-c, and an increase in B12 and E vitamins, folate, and zinc. Additionally, oxidative stress markers also improved significantly. Therefore, improving lifestyle, especially adopting the Mediterranean diet, can improve metabolic and oxidative profiles and enhance overall health-related quality of life.
Article
Biochemistry & Molecular Biology
Alessandra Bettiol, Filippo Fagni, Irene Mattioli, Giacomo Bagni, Gianfranco Vitiello, Alessia Grassi, Chiara Della Bella, Marisa Benagiano, Arianna Troilo, Katarzyna Stella Holownia, David Simon, Flavia Rita Argento, Jurgen Sota, Claudia Fabiani, Matteo Becatti, Claudia Fiorillo, Georg Schett, Giuseppe Lopalco, Luca Cantarini, Domenico Prisco, Elena Silvestri, Giacomo Emmi, Mario Milco D'Elios
Summary: In this study, the ability of serum interleukin (IL)-36a concentration to differentiate Behcet's syndrome (BS) from psoriatic arthritis (PsA) was investigated. The results showed significantly lower IL-36a concentrations in BS patients compared to PsA patients, although both groups had significantly higher IL-36a levels compared to healthy controls. A cut-off of 420.6 pg/mL displayed good specificity and sensitivity in discriminating PsA from BS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Alessandra Bigi, Eva Lombardo, Roberta Cascella, Cristina Cecchi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alessio Biagioni, Sara Peri, Giampaolo Versienti, Claudia Fiorillo, Matteo Becatti, Lucia Magnelli, Laura Papucci
Summary: Blood vessels are crucial for cancer cells to survive and spread in the body, and the formation of abnormal vessel networks is closely related to patients' prognosis. It is urgent to find new pharmaceutical solutions to control and normalize the formation of these networks.
Article
Biochemistry & Molecular Biology
Matteo Becatti, Gianmartin Cito, Flavia Rita Argento, Eleonora Fini, Alessandra Bettiol, Serena Borghi, Amanda Mannucci, Rossella Fucci, Claudia Giachini, Rita Picone, Giacomo Emmi, Niccolo Taddei, Maria Elisabetta Coccia, Claudia Fiorillo
Summary: A significant number of infertile men are classified as idiopathic or unexplained cases due to the lack of clear diagnosis based on standard semen parameters. This study investigates the role of oxidative stress in male infertility and suggests that blood redox markers can be used as an additional tool for assessing sperm quality and providing specific information in preconception care.
Article
Biochemistry & Molecular Biology
Michela Cirillo, Flavia Rita Argento, Matteo Becatti, Claudia Fiorillo, Maria Elisabetta Coccia, Cinzia Fatini
Summary: This study suggests that dietary changes according to the Mediterranean Diet (MD) may have a positive effect on pain perception in endometriosis and its relationship with oxidative stress. The findings indicate that MD could be a new effective strategy for treating symptoms and managing chronic pain in endometriosis in the long term.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Silvia Errico, Giacomo Lucchesi, Davide Odino, Enass Youssef Osman, Roberta Cascella, Lorenzo Neri, Claudia Capitini, Martino Calamai, Francesco Bemporad, Cristina Cecchi, William A. Kinney, Denise Barbut, Annalisa Relini, Claudio Canale, Gabriella Caminati, Ryan Limbocker, Michele Vendruscolo, Michael Zasloff, Fabrizio Chiti
Summary: Natural aminosterols are potential drugs for treating neurodegenerative diseases, and their protective mechanism involves binding to biological membranes and inhibiting amyloidogenic proteins. Three chemically different aminosterols were compared and found to have different binding affinities, charge neutralizations, mechanical reinforcements, and lipid redistributions within liposomes. They also exhibited varying potency in protecting cell membranes against amyloid-beta oligomers. Overall analysis provided an analytical equation that quantitatively described the protective effects of aminosterols based on their concentration and membrane effects, linking their chemistry to membrane protection.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)