Article
Engineering, Marine
Yinyin Deng, Cuicui Tian, Chuanming Hu, Guangping Xu, Lien Yang, Qinqin Lu, Wei Zhou
Summary: The germplasm bank of economic algae serves as a biological insurance for the cultivation industry, but the red algal germplasm is easily contaminated by filamentous cyanobacteria, hampering its growth. This study utilized cytological observations, 16S rRNA gene analysis, and microscopy to investigate the contamination pattern. It was found that the filamentous cyanobacteria mainly originated from the red algal thallus or surrounding seawater.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Gastroenterology & Hepatology
Darja Nikitina, Konrad Lehr, Ramiro Vilchez-Vargas, Laimas Virginijus Jonaitis, Mindaugas Urba, Juozas Kupcinskas, Jurgita Skieceviciene, Alexander Link
Summary: This study characterized the microbiota of gastric cancer using 16S rRNA gene and its transcript and identified differences in bacterial composition. The results showed that there was only partial overlap between DNA and RNA samples and differences between gastric cancer patients and healthy controls depending on the chosen sequencing modality. Furthermore, RNA sequencing was more sensitive for detecting differences in bacterial richness and specific bacteria. Overall, the choice of sequencing modality affects the characterization of the microbiota and the differences between case and control.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Biodiversity Conservation
Wei Chen, Shuyin Li, Yuanzhao Xu, Ruozhen Geng, Gaofei Song, Peiming Ma
Summary: A coccoid-like cyanobacterium isolated from Caohai Lake in China was characterized and named Cyanodorina ovale based on morphological, electron microscopic, and molecular phylogenetic analyses.
Article
Microbiology
Sophi Marmen, Eduard Fadeev, Ashraf Al Ashhab, Ayana Benet-Perelberg, Alon Naor, Hemant J. Patil, Eddie Cytryn, Yehudit Viner-Mozzini, Assaf Sukenik, Maya Lalzar, Daniel Sher
Summary: Aquaculture facilities such as fishponds have a significant anthropogenic impact on freshwater ecosystems, with high fish biomass leading to increased nutrient load and potential effects on microbial communities and fish health. In an interconnected aquaculture system, natural seasonal cycles have a greater influence on the structure of aquatic bacterial communities compared to aquaculture-related factors. Recurring cyanobacterial blooms in response to different nutrient ratios, possibly influenced by fish feed input magnitude, were observed in the system.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Tian-Shu Zhang, Ya-Ning Zhang, De-Chen Lu, Ya Gong, Zong-Jun Du
Summary: A novel species named Carboxylicivirga marinus sp. nov. was isolated from a sediment sample in Weihai, China, exhibiting specific growth conditions and chemical composition.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biodiversity Conservation
Maria Christodoulou, Matti Wahlsten, Kaarina Sivonen
Summary: Cyanobacteria found in both aquatic and terrestrial environments have been extensively studied in Finland, but the diversity of terrestrial rock-inhabiting cyanobacteria is often ignored. In this study, two Pseudanabaena-like cyanobacterial strains were isolated and characterized using a polyphasic approach. Phylogenetic analyses using both 16S rRNA gene and 16S-23S ITS region revealed better resolution of species relationships, and based on these analyses, two new species were described. The study also highlighted the need for taxonomic revision in the poorly studied cyanobacterial genus Pseudanabaena.
Article
Biodiversity Conservation
Naresh Kumar, Aniket Saraf, Sagarika Pal, Prashant Singh
Summary: In this study, two cyanobacterial strains KUT1-PS and 18C-PS from India were characterized using a polyphasic approach. The morphological characterization indicated that both strains belonged to the genus Cylindrospermum. Phylogenetic analyses at the genus and species levels provided evidence that strain 18C-PS is a representative of Cylindrospermum badium, while strain KUT1-PS is a novel species referred to as Cylindrospermum solincola sp. nov. This study emphasizes the importance of comparing newly sequenced strains with established species for taxonomic interpretations.
Article
Biotechnology & Applied Microbiology
Piaopiao Pan, Yichao Gu, Dong-Lei Sun, Qinglong L. Wu, Ning-Yi Zhou
Summary: The 16S rRNA gene is widely used as a marker to study the evolutionary relationships and microbial composition. However, it has limitations such as variable copy numbers, intragenomic heterogeneity, and low taxonomic resolution, causing biases in estimating microbial diversity. This study analyzed prokaryotic genomes and found that the 16S rRNA gene copy number ranged from 1 to 37 in bacteria and 1 to 5 in archaea, with intragenomic heterogeneity in 60% of genomes. The study also calculated the overestimation and underestimation of microbial diversity using different regions of the 16S rRNA gene.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Jinshuang Bo, Xuezhen Song, Shuai Wang, Zongjun Du
Summary: Strain F6397(T) isolated from marine sediment in Weihai, PR China is a novel species of the genus Winogradskyella, showing optimal growth at 30-33 degrees C, pH 7.5, and in the presence of 3.0% NaCl.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Javier Carmona Jimenez, Angela Caro Borrero, Itzel Becerra-Absalon, Elvira Perona Urizar, Kenia Marquez Santamaria, Pilar Mateo Ortega
Summary: This study describes two new Nostoc species, N. montejanii and N. tlalocii, based on a polyphasic approach that combines morphological, ecological, and genetic characteristics. The two new species showed clear differences in their habitat preferences and possesses distinct morphological features. The 16S rRNA gene sequences and phylogenetic analyses revealed their close relationship to the Nostoc genus.
JOURNAL OF PHYCOLOGY
(2023)
Article
Biodiversity Conservation
Denis Davydov, Anna Vilnet, Irina Novakovskaya, Elena Patova
Summary: The strains of Drouetiella species from a terrestrial biotope in the Arctic and Subarctic areas of European Russia were isolated and characterized using an integrative approach. The study showed the morphological plasticity of Drouetiella species and identified a new taxon. The analysis of 16S-23S ITS rRNA sequences revealed differences in length and nucleotide composition, affecting the hypothetical secondary structures of certain helices.
Article
Microbiology
Itaru Dekio, Ken-Ichi Okuda, Masako Nishida, Susumu Hamada-Tsutsumi, Tomo Suzuki, Shigeru Kinoshita, Hiroto Tamura, Kenichiro Ohnuma, Yoshiyuki Murakami, Yuki Kinjo, Akihiko Asahina
Summary: Cutibacterium modestum is a newly described species in the genus Cutibacterium, predicted to be a minor but common member of the skin microbiome. Biochemical characteristics and genomic comparisons showed high similarity among isolates, with minor differences in MALDI-TOF mass spectrometry spectra. These results suggest the potential for subtyping based on recA gene sequences or MALDI-TOF mass spectrometry in C. modestum isolates.
Article
Microbiology
Farooq Inam, Abdul S. Mumtaz, Muhammad Kaleem, Imran Sajid
Summary: Two diverse taxa of true branching Nostocales were discovered from the oasis of Cholistan, Pakistan. They showed different morphological traits and potential antibacterial, cytotoxic, and anticancer effects.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biodiversity Conservation
Shuheng Li, Denghang Yu, Hang Zhang, Renhui Li, Fangfang Cai
Summary: A new algal species, Cartusia hunanesis sp. nov., was discovered in a stream in Hunan, China. Two strains (ZJJ02 and ZJJ03) were studied using morphology, ecology, and molecular data (16S rRNA gene and ITS region). Compared to the type species Cartusia fontana, C. hunanesis has only one trichome in the sheath and large granules near the cross wall. Phylogenetic analysis based on the 16S rRNA gene revealed that the investigated strains of C. hunanesis are closely related to C. fontana. Additionally, Cartusia was found to cluster with members of the family Oculatellaceae, including the genera Pegethrix and Elainella. The ITS region of C. hunanesis showed significant differences in secondary structure compared to C. fontana, indicating that it is a novel species.
Article
Immunology
Yingmiao Zhang, Yue Fan, Yu Zhan, Hao Wang, Xun Li, Hui Wang, Tian Feng, Lifeng Shi, Jing Wang, Zhongxin Lu
Summary: This study reports the first case of human infection caused by P. anthophila in China, providing a fundamental resource for further investigation of its virulence factors, antibiotic resistance, host-pathogen interactions, and comparative genomics of the Pantoea genus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)