Journal
FORENSIC SCIENCE INTERNATIONAL-GENETICS
Volume 6, Issue 2, Pages 258-262Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2011.06.002
Keywords
Laser capture microdissection; DNA extraction; Short tandem repeat analysis
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Funding
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
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In forensic sciences, short tandem repeat (STR) analysis is a valuable tool in identifying the donor(s) of biological stains. Laser capture microdissection (LCM) can be used as a cell separating technique to isolate specific cell types in mixed samples. An important challenge lies in the development of a DNA isolation method appropriate for laser microdissected cells, as these samples usually contain minute amounts of cells. In this study three different DNA isolation methods for LCM collected cells were compared. The PicoPure DNA extraction method outperformed both other methods (IQTM system and short alkaline method). Consequently, the minimal number of LCM collected cells necessary for STR typing was determined. Using the PicoPure DNA extraction method, full DNA profiles could be obtained from as little as 10 cells. Nevertheless, despite the occurrence of allelic drop out in some of the samples, lower amounts of cells gave rise to useful DNA profiles. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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