Article
Biochemical Research Methods
Josefine Herzog, Gisela Skopp, Frank Musshoff
Summary: This study aimed to monitor alcohol consumption over a period of 6 months by analyzing phosphatidylethanol (PEth) homologues in dried blood spots (DBS) and ethyl glucuronide in hair (EtGH). The participants' daily alcohol intake was recorded using an app. The results showed that PEth had a higher detection rate for alcohol consumption than EtGH and that PEth concentrations quickly responded to changes in drinking behavior.
DRUG TESTING AND ANALYSIS
(2023)
Review
Environmental Sciences
Valentina Triolo, Mario Spano, Roberto Buscemi, Simona Gioe, Ginevra Malta, Marija Caplinskiene, Fabio Vaiano, Elisabetta Bertol, Stefania Zerbo, Giuseppe Davide Albano, Antonina Argo
Summary: Ethyl glucuronide (EtG) is a stable ethanol metabolite that can be measured in hair to assess the influence of different pathological conditions. Liver and kidney diseases, as well as diabetes, have been found to be the main factors affecting EtG levels in hair.
Article
Medicine, Legal
Jennifer P. Pascali, Arianna Giorgetti, Guido Pelletti, Luca Morini, Susan Mohamed, Marta Barbaresi, Rossana Cecchi, Susi Pelotti, Paolo Fais
Summary: This study reported data on alcohol consumption among university students and found that excessive drinking was not prevalent among the student population. The study also revealed a significant level of interest in analyzing and interpreting the data, suggesting that public health promotion activities within universities could be beneficial.
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2023)
Article
Biochemical Research Methods
Catalina Dumitrascu, Celine Gys, Sarah M. R. Wille, Maria del Mar Ramirez-Fernandez, Diona D'Hondt, Alexia Van Goethem, Babette Van Rafelghem, Eline Baetens, Werner Jacobs, Hugo Neels, Adrian Covaci, Alexander L. N. van Nuijs
Summary: Monitoring long-term alcohol use and/or abstinence is crucial. Ethyl glucuronide (EtG) in hair provides information on alcohol consumption over months. Phosphatidylethanol (PEth) 16:0/18:1 analysis in whole blood detects recent alcohol consumption. The analysis of these biomarkers can lead to a more accurate categorisation of individuals, emphasizing the need for two alcohol use biomarkers with different detection windows.
DRUG TESTING AND ANALYSIS
(2023)
Article
Medicine, Legal
Louise Vermeulen, Alexander L. N. van Nuijs, Cleo L. Crunelle, Werner Jacobs, Hugo Neels
Summary: This study compares the EtG concentration in hair and fingernails of teetotalers and proposes a cut-off value for EtG in fingernails for alcohol abstinence. The results suggest that the cut-off value for alcohol abstinence may be lower than the previously suggested concentrations.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Alessandro Mattia, Clementina Moschella, Maria Chiara David, Marco Fiore, Sara Gariglio, Alberto Salomone, Marco Vincenti
Summary: Ethyl glucuronide (EtG) is a minor ethanol metabolite that can be detected in various matrices. This study developed and validated a new method for analyzing EtG in hair samples using gas chromatography and tandem mass spectrometry. The method was successfully applied to real hair samples and used to assess excessive alcohol consumption and verify the psycho-physical requirements of individuals.
FRONTIERS IN CHEMISTRY
(2022)
Review
Gastroenterology & Hepatology
Akshay Shetty, Sergio De La Torre, Brittney Ibrahim, Andrew Hong, Julio Meza, Sammy Saab
Summary: Alcohol-related liver disease has increased over the past 20 years, leading to a greater need for accurate assessment of alcohol use. Poor outcomes have been associated with alcohol use in both pre-liver transplant and post-liver transplant patients. Patients with alcohol use disorder often underestimate their alcohol consumption, highlighting the importance of objective assessment. Multiple serologic biomarkers are available to assist clinicians in assessing recent alcohol consumption, and this review evaluates their strengths and weaknesses in the pre-liver transplant and post-liver transplant population.
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2023)
Article
Biochemical Research Methods
Vanessa Hofmann, Tom R. Sundermann, Georg Schmitt, Marc Bartel
Summary: This study developed a new analytical method for the simultaneous determination of four alcohol biomarkers in human blood, meeting forensic requirements and demonstrating linearity, selectivity, and sensitivity.
DRUG TESTING AND ANALYSIS
(2022)
Article
Surgery
N. Lim, T. M. Leventhal, M. J. Thomson, M. Hassan, J. Thompson, A. Adams, S. Chinnakotla, V Humphreville, R. Kandaswamy, V Kirchner, T. L. Pruett, L. Schuller, M. McCarty, J. Lake
Summary: This study examines the use of urine EtG and serum PEth in alcohol screening protocols for patients with alcohol use disorder before and after liver transplant. The findings suggest that protocol adherence is higher when using PEth compared to EtG. While biomarker screening can detect recurrent alcohol use in this population, engaging patients into chemical dependency treatment remains challenging.
CLINICAL TRANSPLANTATION
(2023)
Review
Public, Environmental & Occupational Health
Przemyslaw Paul, Katarzyna Kanclerz, Alicja Kubanek, Joanna Renke, Marcin Renke
Summary: Alcohol abuse poses significant threats to health and life, and the use of biochemical markers can help in early detection, effective therapy, and reliable control of abstinence.
Article
Multidisciplinary Sciences
Alice Cerioni, Erika Buratti, Gianmario Mietti, Marta Cippitelli, Mariano Cingolani, Rino Froldi, Roberto Scendoni
Summary: Larvae and insects serve as important alternative biological matrices in postmortem forensic toxicology. This study developed and validated an analytical method for the quantification of Ethyl glucuronide (EtG) in larvae using Ultra High-Performance Liquid Chromatography at High Resolution Mass Spectrometry (HPLC/HR-MS). The method successfully identified EtG in the larvae of Lucilia sericata (Calliphoridae) found on a highly decomposed body.
Article
Psychiatry
Eugenio Alladio, Lia Visintin, Tonia Lombardo, Roberto Testi, Alberto Salomone, Marco Vincenti
Summary: The study found an increase in the percentage of abstinent/low-risk drinkers and a decrease in moderate and chronic drinkers among the subjects surveyed from April to August 2020. During the pandemic, some individuals showed an increase in alcohol consumption, especially chronic/excessive drinkers and female consumers.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Obstetrics & Gynecology
Mirjami Jolma, Mikko Koivu-Jolma, Onni Niemela, Ilona Autti-Ramo, Hanna Kahila
Summary: The study aimed to evaluate the potential of using a rapid screening test to measure ethyl glucuronide (EtG) in urine samples of pregnant women. The findings suggest that rapid EtG tests may be a simple and inexpensive method to screen alcohol use among pregnant women during routine prenatal visits.
BMC PREGNANCY AND CHILDBIRTH
(2023)
Article
Substance Abuse
Shayani Ghosh, Raka Jain, Ravindra Rao, Ashwani Kumar Mishra, Sonali Jhanjee
Summary: This study investigated the association between self-reported alcohol consumption and traditional biomarkers (GGT, AST, ALT, CDT, and MCV) as well as a direct biomarker, hair EtG, in patients with alcohol dependence syndrome. The results showed that hair EtG was significantly correlated with alcohol consumption, while traditional biomarkers showed no correlation. Therefore, hair EtG can be used to estimate long-term alcohol consumption and detect heavy drinkers among individuals with alcohol dependence syndrome.
Article
Pharmacology & Pharmacy
Gudrun Hoiseth, Thor Hilberg, Torleif Trydal, Asgeir Husa, Vigdis Vindenes, Stig Tore Bogstrand
Summary: This study evaluated the quantitative relation between common clinical chemical analyses and ethanol use, measured by PEth and CDT. Results showed a strong relation between HDL-C, AST, ferritin, and GGT levels and alcohol consumption as measured by PEth. Elevated levels of these clinical chemical analytes should prompt further investigation into possibly harmful alcohol use.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2022)
Review
Medical Laboratory Technology
Paul J. Jannetto, Anders Helander, Uttam Garg, Gregory C. Janis, Bruce Goldberger, Hemamalini Ketha
CLINICAL CHEMISTRY
(2019)
Article
Toxicology
Matilda Backberg, Madeleine Pettersson Bergstrand, Olof Beck, Anders Helander
CLINICAL TOXICOLOGY
(2019)
Letter
Toxicology
Lisa Franzen, Olof Beck, Anders Helander
CLINICAL TOXICOLOGY
(2019)
Article
Substance Abuse
Anders Helander, Michael Bottcher, Norbert Dahmen, Olof Beck
ALCOHOL AND ALCOHOLISM
(2019)
Article
Veterinary Sciences
L. B. Christiansen, S. E. Cremer, A. Helander, Tine Madsena, M. J. Reimann, J. E. Moller, K. Hoglund, I Ljungvall, J. Haggstrom, L. H. Olsen
VETERINARY JOURNAL
(2019)
Article
Substance Abuse
Jasna Neumann, Olof Beck, Anders Helander, Michael Boettcher
ALCOHOL AND ALCOHOLISM
(2020)
Article
Biochemistry & Molecular Biology
Qian Yu, Bao Khanh Lai, Parvin Ahooghalandari, Anders Helander, Erik Gylfe, Patrick Gilon, Anders Tengholm
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Anders Helander, Matilda Backberg, Olof Beck
Article
Medical Laboratory Technology
Jasna Neumann, Olof Beck, Anders Helander, Norbert Dahmen, Michael Boettcher
JOURNAL OF LABORATORY MEDICINE
(2020)
Article
Biochemical Research Methods
Olof Beck, Maria Mellring, Christian Lowbeer, Sabina Seferaj, Anders Helander
Summary: PEth is a specific alcohol biomarker formed in cell membranes after alcohol consumption, but formation may continue in blood samples containing ethanol, leading to potentially inaccurate results. Use of a PLD inhibitor is recommended to prevent post-sampling formation of PEth.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Anders Helander, Malin Johansson, Annika Andersson, Tomas Villen
Summary: A new interfering peak was discovered during routine urine drug testing for cannabis at the Karolinska University Laboratory in Sweden, which was identified as delta-8-THC-COOH and caused analytical and medico-legal concerns.
DRUG TESTING AND ANALYSIS
(2022)
Article
Orthopedics
Julie Weber Melchior Egholm, Bolette Pedersen, Kristian Oppedal, Bjorn Lindegard Madsen, Jes Bruun Lauritzen, Mette Rasmussen, Anders Helander, Johanna Adami, Hanne Tonnesen
Summary: This study found that intensive alcohol intervention before surgery can increase the rate of alcohol cessation in patients, but showed no significant effect on long-term low-risk drinking. There were no significant differences in postoperative complications, quality of life, and hospital costs between the two groups.
Article
Biochemical Research Methods
Anders Helander, Alexia Rylski
Summary: A study found that the use of the traditional medicine kratom is increasing in Swedish society. An improved analytical method enables the identification and quantification of various diastereomers in kratom, which is important for understanding the health risks and legal aspects of kratom.
DRUG TESTING AND ANALYSIS
(2023)
Letter
Biochemical Research Methods
Frank Musshoff, Michael Boettcher, Matthias Graw, Gisela Skopp, Jasna Neumann, Gudrun Hoiseth, Anders Helander
DRUG TESTING AND ANALYSIS
(2023)
Article
Medicine, Research & Experimental
Anders Helander, Therese Hansson
Summary: Phosphatidylethanol (PEth) is a sensitive and specific alcohol biomarker that accumulates in the blood during repeated ethanol exposure and is slowly eliminated during abstinence. Its use has significantly increased over the past decade. In Sweden, routine limits and interpretation of PEth test results were harmonized, and an external quality assessment scheme for PEth measurement was initiated. PEth values below the reporting limit indicate sobriety or low alcohol consumption, while values above 0.30 μmol/L typically indicate regular high alcohol intake.
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
(2023)
Article
Medicine, Legal
Heitor S. D. Correa, Ivano Alessandri, Andrea Verzeletti
Summary: This research assessed the usefulness of Raman spectroscopy and gas chromatography-mass spectrometry in analyzing bones. The techniques were found to be useful in molecular taphonomy studies and forensic genetics.
FORENSIC SCIENCE INTERNATIONAL
(2024)
