Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Microbiology
Peng Fei, He Jing, Yan Ma, Gege Dong, Yunhe Chang, Zhaoxu Meng, Shilong Jiang, Qinggang Xie, Shuzhen Li, Xi Chen, Weiwei Yang
Summary: The study investigated the prevalence and characteristics of Cronobacter spp. in commercial powdered infant formula (PIF) from nine provinces in China. It found that Cronobacter spp. contamination was common in PIF, with the main sequence types being ST4, ST1, and ST64, and the dominant serotype being C. sakazakii serotype O2. Most isolates were susceptible to commonly used antibiotics, but showed resistance to cephalothin and vancomycin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Sergio Acuna, Sarah Lepuschitz, Ariane Pietzka, Alejandra Contreras-Fernandez, Pamela Chavarria-Sepulveda, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Alejandro Castillo, Werner Ruppitsch, Stephen Forsythe
Summary: This study characterized Cronobacter and Salmonella strains isolated from powdered infant formula samples in Chile. The Cronobacter strains exhibited antibiotic resistance and possessed virulence-associated genes, while the Salmonella strains showed antibiotic resistance and harbored numerous virulence-associated genes. The presence of these strains in powdered infant formula poses a potential risk to infant health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Lihan Wang, Xinyi Pang, Jiayuan Zhao, Haonan Jin, Xinyan Yang, Shiqian Fu, Shasha Cheng, Hongxuan Li, Chao Miao, Chaoxin Man, Yujun Jiang
Summary: JK004 is a novel virulent phage isolated from the native environment of C. sakazakii, known for its tolerance to heat, pH, and osmotic-stress. It shows excellent antibacterial activity against C. sakazakii and biofilms, without containing any known virulence, transduction, or antibiotic resistance genes, indicating its safety for practical applications in food production. The study suggests the potential of JK004 as an effective strategy for controlling C. sakazakii.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Hye Ri Kim, Byoung Chan Kim
Summary: In this study, a newly proposed sequential partitioning method was used to isolate specific aptamers for all seven species of Cronobacter. This method avoided repeated enrichment steps and reduced the selection time compared to the conventional SELEX process. Four aptamers with high affinity and specificity for Cronobacter were successfully isolated, and they could effectively detect Cronobacter in contaminated PIF.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Dandan Luo, Chengsi Li, Qingping Wu, Yu Ding, Meiyan Yang, Yongdan Hu, Haiyan Zeng, Jumei Zhang
Summary: Cronobacter spp. phage vB_CtuP_A24, a novel lytic phage with the ability to lyse five Cronobacter species, shows stability under various environmental conditions and potential as a promising biocontrol agent against Cronobacter spp. in food production.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Chemistry, Analytical
Stephen Erickson, Jose Gil, Jessica Stach, Wendy Hahn, Minh M. Nguyen
Summary: The study validated the effectiveness of the PhageDx Cronobacter assay for detecting Cronobacter in powdered infant formula, showing no significant difference compared to ISO and FDA methods. The assay demonstrated specificity, robustness, consistency, and stability.
JOURNAL OF AOAC INTERNATIONAL
(2021)
Article
Agriculture, Dairy & Animal Science
Ju Liu, Guoyang Xie, Qin Xiong, Dan Mu, Hengyi Xu
Summary: A simple and sensitive fluorescent turn-off aptasensor was developed for detecting viable Cronobacter sakazakii in powdered infant formula. The sensor showed good performance and specificity in detecting the pathogen within 3 hours in both pure culture and formula samples.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Danliangmin Song, Xuehe Qi, Yan Huang, Ai Jia, Yaqi Liang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: Cronobacter species are opportunistic foodborne pathogens that pose a threat to infant health. This study investigates the relationship between antibiotic tolerance and virulence in Cronobacter, and identifies proteins implicated in virulence and antibiotic resistance through proteomics analysis. The findings suggest that antibiotic tolerance in Cronobacter is associated with increased production of enterotoxin and hemolysin, and certain strains exhibit decreased utilization of sialic acid. The study also reveals different proteomes and virulence-related proteins in strains with different antibiotic tolerance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Yuan-song Zhang, Xuechao Xu, Juanli Yang, Mengyuan Tan, Wenyuan Zhou, Lu Gao, Zhen-quan Yang
Summary: In this study, a chromogenic system based on phage-directed immobilized nanozyme was developed for colorimetric measurement of Cronobacter sakazakii in powdered infant formula (PIF). The method showed high specificity and low detection limit, successfully detecting C. sakazakii and achieving good recovery rates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Bao Gao, Tongtong Ma, Lin Feng, Xiaolin Huang, Xuelan Chen, Yonghua Xiong
Summary: In this study, a novel immunosensor using the light scattering intensity of silver and gold alloy as a signal transducer was developed to enhance the sensitivity and linearity for quantitative detection of Cronobacter muytjensii. The developed method showed high sensitivity with a low detection limit, wide dynamic range, and acceptable accuracy for quantitative detection of C. muytjensii in real PIF samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Agriculture, Dairy & Animal Science
Lihan Wang, Stephen J. Forsythe, Xinyan Yang, Shiqian Fu, Chaoxin Man, Yujun Jiang
Summary: Cronobacter genus includes food-borne pathogens that can cause severe infections in infants, with a high mortality rate. Advanced detection technology and analysis of high-frequency species from different sources can aid in targeted control of Cronobacter. The bacteria's resistance to environmental stresses and the development of new control techniques highlight the importance of strict monitoring and prevention throughout food processing and storage.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Geng Zou, Libere Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li, Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li
Summary: In this study, a phage termed LPCS28 with a broad lysis spectrum against C. sakazakii was isolated from environmental water samples. LPCS28 showed high thermal stability, with no significant decrease in titer after heating at 60 degrees C for one hour. Upon thermal processing at 63 degrees C for 30 min, LPCS28 effectively inhibited C. sakazakii in reconstituted powdered infant formula and liquid milk, reducing its concentration to below the limit of detection within 9 h at 37 degrees C. Phylogenetic analysis classified LPCS28 as a new genus, Nanhuvirus, within the family Straboviridae. These results highlight the potential of LPCS28 as a biological control agent against pathogenic C. sakazakii in the dairy industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Microbiology
Sambit K. Das, Sanath H. Kumar, Binaya B. Nayak, Manjusha Lekshmi
Summary: This study investigated the incidence of Cronobacter spp. in seafood from retail fish markets in Mumbai, India. It found the highest incidence of Cronobacter spp. in dried fish, with Cronobacter malonaticus being the most common species identified. The detection of Cronobacter spp. highlights the importance of monitoring this emerging pathogen in tropical seafood.
CURRENT MICROBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Dong Yan, Jingming Xin, Weiping Li, Sha Liu, Hongbin Wu, Wei Ma, Jiannian Yao, Chuanlang Zhan
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Chemistry, Applied
Xiaofang Li, Huifeng Meng, Fugang Shen, Dan Su, Shuying Huo, Jinhuan Shan, Jianhua Huang, Chuanlang Zhan
Article
Chemistry, Physical
Kun Li, Yishi Wu, Yabing Tang, Ming-Ao Pan, Wei Ma, Hongbing Fu, Chuanlang Zhan, Jiannian Yao
ADVANCED ENERGY MATERIALS
(2019)
Article
Polymer Science
Weiping Li, Wenxu Liu, Xin Zhang, Dong Yan, Feng Liu, Chuanlang Zhan
MACROMOLECULAR RAPID COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Xiaofang Li, Kun Li, Dan Su, Fugang Shen, Shuying Huo, Hongbing Fu, Chuanlang Zhan
CHINESE CHEMICAL LETTERS
(2020)
Article
Nanoscience & Nanotechnology
Huifeng Meng, Yongchun Li, Bo Pang, Yuqing Li, Ying Xiang, Liang Guo, Xuemei Li, Chuanlang Zhan, Jianhua Huang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Multidisciplinary
Yuan Chang, Tsz-Ki Lau, Ming-Ao Pan, Xinhui Lu, He Yan, Chuanlang Zhan
MATERIALS HORIZONS
(2019)
Article
Chemistry, Physical
Ming-Ao Pan, Tsz-Ki Lau, Yabing Tang, Yi-Ching Wu, Tao Liu, Kun Li, Ming-Chou Chen, Xinhui Lu, Wei Ma, Chuanlang Zhan
JOURNAL OF MATERIALS CHEMISTRY A
(2019)
Article
Energy & Fuels
Tao Liu, Wei Gao, Guangye Zhang, Lin Zhang, Jingming Xin, Wei Ma, Chuluo Yang, He Yan, Chuanlang Zhan, Jiannian Yao
Article
Chemistry, Multidisciplinary
Tao Liu, Zhenghui Luo, Yuzhong Chen, Tao Yang, Yiqun Xiao, Guangye Zhang, Ruijie Ma, Xinhui Lu, Chuanlang Zhan, Maojie Zhang, Chuluo Yang, Yongfang Li, Jiannian Yao, He Yan
ENERGY & ENVIRONMENTAL SCIENCE
(2019)
Article
Materials Science, Multidisciplinary
Huifeng Meng, Yongchun Li, Bo Pang, Yuqing Li, Chuanlang Zhan, Jianhua Huang
JOURNAL OF MATERIALS CHEMISTRY C
(2019)
Article
Materials Science, Multidisciplinary
Min Zeng, Chuanlang Zhan, Jiannian Yao
JOURNAL OF MATERIALS CHEMISTRY C
(2019)
Article
Chemistry, Multidisciplinary
Fugang Shen, Dong Yan, Weiping Li, Huifeng Meng, Jianhua Huang, Xuemei Li, Jianzhong Xu, Chuanlang Zhan
MATERIALS CHEMISTRY FRONTIERS
(2019)
Article
Chemistry, Physical
Monika Gupta, Yan Dong, Shen Fugang, Xu Jianzhong, Zhan Chuanlang
ACTA PHYSICO-CHIMICA SINICA
(2019)
Article
Chemistry, Multidisciplinary
Monika Gupta, Dong Yan, Jiannian Yao, Chuanlang Zhan
MATERIALS CHEMISTRY FRONTIERS
(2018)