Journal
FOOD SCIENCE AND TECHNOLOGY RESEARCH
Volume 17, Issue 2, Pages 161-166Publisher
KARGER
DOI: 10.3136/fstr.17.161
Keywords
miso koji mold; Aspergillus oryzae; gene targeting; ku70 gene
Categories
Funding
- Agriculture Forestry and Fisheries
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A homologous gene replacement system was developed for an industrial strain of Aspergillus oryzae KBN630 used in miso production. This system was based on a transformation method involving a bidirectional marker gene, pyrG. To increase the integration of exogenous DNA fragments by homologous recombination, the ku70 gene encoding the Ku70 component of the nonhomologous end-joining machinery was removed from the genome of a pyrG deletion mutant derived from A. oryzae KBN630. The efficiency of homologous integration at the amyR locus was more than 90%.
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