Article
Biochemistry & Molecular Biology
Laura Darie-Ion, Madhuri Jayathirtha, Gabriela Elena Hitruc, Marius-Mihai Zaharia, Robert Vasile Gradinaru, Costel C. Darie, Aurel Pui, Brindusa Alina Petre
Summary: Through combining different analytical methods, zein protein was extracted including mass spectrometry, SDS-PAGE, AFM, and FTIR-ATR. The use of ultrasonic extraction method with 65% ethanol extraction buffer and 710 mu m maize granulation yielded the highest protein extraction. The extracted zein was mainly alpha-zein monomer according to SDS-PAGE analysis and the main component in maize flour extract was alpha-zein PMS2 protein based on MS analysis. A homogeneous content of extracted zein could potentially be used for future medical applications according to AFM studies.
Article
Biochemistry & Molecular Biology
Roman Mylonas, Alexandra Potts, Patrice Waridel, Jachen Barblan, Maria del Carmen Conde Rubio, Christian Widmann, Manfredo Quadroni
Summary: The native molecular weight of proteins is an important characteristic, and SDS-PAGE is a commonly used technique for protein separation and molecular weight determination. However, electrophoresis results are often reproducible and inaccurate, impacting antibody validation and western blot data reliability. To address these issues, we propose establishing a database of reference molecular weights measured by SDS-PAGE. Using mass spectrometry as an orthogonal detection method, we obtained electrophoretic migration patterns for approximately 100,000 human proteins and determined accurate and reproducible molecular weights. This database can be accessed freely as a web resource and is expected to be useful for troubleshooting western blot experiments and characterizing human proteoforms.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Analytical
Zahra Ghafoori, Tahereh Tehrani, Laura Pont, Fernando Benavente
Summary: The protein profiling of whey and casein proteins in bovine milk is important in food science and technology. This study presents a novel and simple capillary electrophoresis-mass spectrometry method for the separation and identification of these proteins within 15 minutes. High-resolution mass spectrometry is shown to be valuable in characterizing different protein proteoforms in bovine milk accurately.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Weiping Jin, Yijun Pan, Yi Wu, Chunling Chen, Wei Xu, Dengfeng Peng, Qingrong Huang
Summary: Camellia oleifera seed oil, with nearly 80% oleic acid, stored in the subcellular oil bodies (OBs) organelles, showed better stability when extracted under alkaline conditions and at high temperatures. The analysis revealed that oleosins and coextracted proteins played a role in the stability of OBs.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Ayako Takemori, Philipp T. Kaulich, Liam Cassidy, Nobuaki Takemori, Andreas Tholey
Summary: The combination of liquid chromatography (LC) and gas-phase separation by field-asymmetric ion mobility spectrometry (FAIMS) is a powerful proteoform separation system for top-down proteomics. In this study, an in-depth top-down proteomics workflow, GeLC-FAIMS-MS, was presented, which utilized a molecular-weight-based proteome fractionation approach using SDS-poly-acrylamide gel electrophoresis. The authors optimized the MS parameters for each gel band and compensating voltage (CV) to improve the identification of proteoforms. They also developed a simple and cost-effective method, combining the PEPPI protocol with an AnExSP method, for stain and SDS removal. Results showed that this method was better suited for low-molecular-weight proteoforms, while a methanol-chloroform-water (MCW)-based protocol showed advantages for higher-molecular-weight proteoforms.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Deseree J. Reid, Tapasyatanu Dash, Zhihan Wang, Craig A. Aspinwall, Michael T. Marty
Summary: Daptomycin is a cyclic lipopeptide antibiotic that interacts with lipid membranes in a non-specific manner. The interaction strength depends on membrane rigidity, with more rigid membranes showing stronger interactions. More fluid membranes may undergo pore formation to expose daptomycin to oxidation. These findings highlight the complementary use of native mass spectrometry, fast photochemical oxidation of peptides, and membrane conductance experiments in studying antibiotic-membrane interactions.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Ruben Agregan, Noemi Echegaray, Maria Lopez-Pedrouso, Radwan Kharabsheh, Daniel Franco, Jose M. Lorenzo
Summary: Proteomics has made significant advances in the field of medicine and has applications in other scientific areas, such as the study of milk. Milk proteomics can help characterize milk samples and products in detail, with significant applications in industry for quality control and improvement.
Article
Biochemical Research Methods
Kevin Hooijschuur, Xiufen Liu, Anna Grootendorst, Ines Pieterman, Javier Sastre Torano
Summary: The use of mixed ion exchange particles and diatomite allows for high-capacity analysis of low-abundant compounds in CZE-DTIMS-MS, increasing sample injection volumes and flow rates.
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hiba Salim, Laura Pont, Estela Gimenez, Fernando Benavente
Summary: This study presents an online aptamer affinity solid-phase extraction direct mass spectrometry (AA-SPE-MS) method for the rapid purification, preconcentration, and characterization of alpha-synuclein (alpha-syn), a protein biomarker related to Parkinson's disease. The method utilizes SPE microcartridges and replaces the separation voltage with a pressure of 100 mbar to elute the protein towards the mass spectrometer. The developed method shows good repeatability, satisfactory linearity, and lower limits of detection compared to other techniques.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jiyeon Lee, Dahye Im, Yajie Liu, Jing Fang, Xibao Tian, Minsu Kim, Wen-Bin Zhang, Jongcheol Seo
Summary: A technique combining ion mobility spectrometry-mass spectrometry (IMS-MS) and supercharging electrospray ionization (ESI) has been demonstrated to effectively differentiate protein chemical topology. By incorporating as many charges as possible into proteins via supercharging ESI, the protein chains can be largely unfolded and stretched, revealing their hidden chemical topology. Different chemical topologies result in different geometric sizes of the unfolded proteins due to constraints in torsional rotations. The introduction of new topological indices allows for successful differentiation of various protein chemical topologies. This technique provides an easy classification of the topological features of complex proteins, previously concealed by their inherent complexity, and establishes a visual standard for discerning protein topologies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Lu Li, Cuiji Sun, Yaoting Sun, Zhen Dong, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, Shang Cai, Hongguang Xia, Yi Zhu, Tiannan Guo, Kiryl D. Piatkevich
Summary: This paper describes a spatially resolved proteomics method called Expansion Proteomics (ProteomEx), which combines tissue expansion with mass spectrometry-based proteomics. The method allows quantitative profiling of proteome variability in mammalian tissues using manual microsampling, without the need for custom equipment. The authors validated the method by analyzing brain, liver, and breast cancer samples, and demonstrated its utility by identifying proteins associated with Alzheimer's disease in a mouse model.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Roderick Corstiaan Abraham Versloot, Patricia Arias-Orozco, Matthijs Jonathan Tadema, Florian Leonardus Rudolfus Lucas, Xinghong Zhao, Siewert J. J. Marrink, Oscar Paul Kuipers, Giovanni Maglia
Summary: Mass spectrometry cannot distinguish between molecules with the same mass-to-charge ratio, but nanopore technology can differentiate diastereomeric and enantiomeric differences in peptides that cannot be observed by conventional MS analysis. The resolution in nanopore analysis is likely due to multiple chiral interactions during peptide transport across the nanopore, similar to chiral chromatography. Nanopore technology also offers possibilities for the rapid and cost-effective analysis of peptides, including those that cannot be easily differentiated by mass spectrometry.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Lukasz Pawel Kozlowski
Summary: Proteome-pI 2.0 is an online database that predicts the isoelectric points and pKa values of proteins and peptides, catering to analytical biochemistry and proteomics techniques. The database includes over 61 million protein sequences and utilizes 21 methods to predict the isoelectric point of proteins and one method for inferring pKa values, facilitating bottom-up proteomics analysis.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Food Science & Technology
Katarzyna Dymek, Lea Rems, Barbara Zorec, Petr Dejmek, Federico Gomez Galindo, Damijan Miklavcic
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2015)
Article
Food Science & Technology
J. Borjesson, P. Dejmek, R. Lofgren, M. Paulsson, M. Glantz
INTERNATIONAL DAIRY JOURNAL
(2015)
Article
Chemistry, Physical
Ali Marefati, Marilyn Rayner, Anna Timgren, Petr Dejmek, Malin Sjoo
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2013)
Article
Chemistry, Physical
Maria Matos, Anna Timgren, Malin Sjoo, Petr Dejmek, Marilyn Rayner
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2013)
Article
Chemistry, Physical
Marilyn Rayner, Diana Marku, Madeleine Eriksson, Malin Sjoo, Petr Dejmek, Marie Wahlgren
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2014)
Article
Chemistry, Physical
Marilyn Rayner, Malin Sjoo, Anna Timgren, Petr Dejmek
FARADAY DISCUSSIONS
(2012)
Article
Food Science & Technology
Katarzyna Dymek, Petr Dejmek, Federico Gomez Galindo
FOOD AND BIOPROCESS TECHNOLOGY
(2014)
Article
Chemistry, Applied
Lucas Daniel Tivana, Jose Da Cruz Francisco, Felix Zelder, Bjorn Bergenstahl, Petr Dejmek
Article
Food Science & Technology
Valentina Panarese, Petr Dejmek, Pietro Rocculi, Federico Gomez Galindo
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2013)
Article
Food Science & Technology
Sanja Seratlic, Branko Bugarski, Viktor Nedovic, Zorica Radulovic, Lars Wadso, Petr Dejmek, Federico Gomez Galindo
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2013)
Article
Agriculture, Multidisciplinary
Marilyn Rayner, Anna Timgren, Malin Sjoo, Petr Dejmek
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2012)
Article
Food Science & Technology
Katarzyna Dymek, Petr Dejmek, Valentina Panarese, Antonio A. Vicente, Lars Wadso, Christine Finnie, Federico Gomez Galindo
LWT-FOOD SCIENCE AND TECHNOLOGY
(2012)
Article
Food Science & Technology
Lucas Daniel Tivana, Petr Dejmek, Bjorn Bergenstahl
Article
Food Science & Technology
Irene Stuart Torrie de Carvalho, Yvonne Granfeldt, Ann-Charlotte Eliasson, Petr Dejmek
Article
Biotechnology & Applied Microbiology
Sanja Seratlic, Branko Bugarski, Zorica Radulovic, Petr Dejmek, Lars Wadso, Viktor Nedovic
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2013)
Article
Food Science & Technology
Yonghong Ye, Songyan Zheng, Yuanxing Wang
Summary: In this study, the changes of aroma components in Gannan navel orange during growth were systematically studied using HS-SPME-GC-MS. Key aroma components and markers were identified. These findings contribute to a better understanding of the dynamic variation of aroma compounds during navel orange growth and have potential for industrial applications.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Jia Xu, Yayuan Zhang, Mengke Zhang, Xinlin Wei, Yiming Zhou
Summary: This study evaluated the effects of glucosamine selenium on summer-autumn tea, showing that it can enhance nutritional quality, improve sensory characteristics, and increase plant adaptation to environmental changes and abiotic stresses.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Yuanming Chu, Zhaoyang Ding, Jing Xie
Summary: This study investigated the use of ice glazing containing D-sodium erythorbate (DSE) and vacuum packaging to maintain the quality of large yellow croaker during frozen storage. The results showed that vacuum packaging effectively inhibited ice crystal growth and minimized water loss, while the combination of vacuum packaging and 0.3% DSE-infused ice glazing maintained freshness indicators at low levels throughout the 300-day storage period. This combination was considered the most effective in preserving the quality of the fish.
FOOD RESEARCH INTERNATIONAL
(2024)
Review
Food Science & Technology
Md. Ashikur Rahman, Shirin Akter, Md. Ashrafudoulla, Md. Anamul Hasan Chowdhury, A. G. M. Sofi Uddin Mahamud, Si Hong Park, Sang-Do Ha
Summary: This comprehensive review aims to provide insights into the mechanisms and key factors influencing biofilm formation by A. hydrophila in the food industry. It explores the molecular processes involved in various stages of biofilm formation and investigates the impact of intrinsic factors and environmental conditions on biofilm architecture and resilience. The article also highlights the potential of bibliometric analysis in conceptualizing the research landscape and identifying knowledge gaps in A. hydrophila biofilm research.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Chujing Wang, Wenni Tian, Zengliu Song, Qun Wang, Yong Cao, Jie Xiao
Summary: Solid lipid ratio in emulsions has significant effects on colloidal stability, mucus permeability, and bioavailability in vivo. Higher solid lipid ratio improves intestinal stability but reduces mucus permeability.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Dacai Zhong, Liping Kang, Juan Liu, Xiang Li, Li Zhou, Luqi Huang, Zidong Qiu
Summary: In this study, a novel online extraction electrospray ionization mass spectrometry method was developed to comprehensively characterize complex foods. Meanwhile, a characteristic marker screening method and chemometrics modeling were used for the accurate authentication of highly-similar foods. This research has significant implications for ensuring food quality and safety.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Qiannan Zhao, Jinyi Yang, Jiahui Li, Lei Zhang, Xiaohai Yan, Tianli Yue, Yahong Yuan
Summary: This study investigated the transport and hypoglycemic effects of phenolics in mulberry leaves using an in vitro digestion model. The results showed that digested phenolics had higher absorbability and could inhibit glucose digestion and absorption. Phenolics also regulated glucose metabolism. Luteoforol and p-coumaric acid were found to be the primary phenolics strongly correlated with the hypoglycemic ability of mulberry leaves.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Yanyun Cao, Qingling Wang, Jinou Lin, Yin-Yi Ding, Jianzhong Han
Summary: This study investigated the effects of gallic acid (GA) and epigallocatechin gallate (EGCG) at different ratios on the gel properties of calcium induced-whey protein emulsion gel. It was found that GA and EGCG could promote gel formation, increase gel strength, and delay the release of emulsified oil droplets.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Monique Martins Strieder, Vitor Lacerda Sanches, Mauricio Ariel Rostagno
Summary: This study proposed an integrated and automated procedure for extracting, separating, and quantifying bioactive compounds from coffee co-products. By optimizing the extraction, separation, and analysis method, real-time analysis was achieved. The results suggest that coupling different techniques can efficiently extract, separate, and analyze phenolic compounds, providing an integrated method to produce high-added value ingredients for various applications.
FOOD RESEARCH INTERNATIONAL
(2024)
Article
Food Science & Technology
Kyu Sang Sim, Hyoyoung Kim, Suel Hye Hur, Tae Woong Na, Ji Hye Lee, Ho Jin Kim
Summary: Geographical origin plays a crucial role in determining the quality and safety of agricultural products. In this study, ICP analysis was used to determine the inorganic elemental content of onions and identify their geographical origin. Chemometric methods were applied to analyze the ICP results, and the accuracy of distinguishing between Korean and Chinese onions was found to be excellent. The findings suggest that this method can be beneficial for identifying agricultural products.
FOOD RESEARCH INTERNATIONAL
(2024)
