Journal
FOOD CONTROL
Volume 25, Issue 2, Pages 654-659Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2011.11.032
Keywords
Intense pulsed light (IPL); UVC; Listeria monocytogenes; Escherichia coli O157:H7; Cell damage
Categories
Funding
- Ewha Womans University
- Fisheries Specific Research & Development Program [F20800308H220000111]
- Ministry of Maritime Affairs and Fisheries of Korean government
- Korea Food & Drug Administration [10162KFDA995]
- Food & Drug Administration (KFDA), Republic of Korea [10162기후식995-2030] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (iPET), Republic of Korea [IPET310016-3] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Korea Institute of Marine Science & Technology Promotion (KIMST) [F20800308H220000100] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Food & Drug Safety (MFDS), Republic of Korea [10162기후식995] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The purpose of this study was to compare the degree of microbial inactivation and cell damage induced by intense pulsed light (IPL) and short-wavelength ultraviolet (UVC) in Listeria monocytogenes and Escherichia coli O157:H7. The viability of the food-borne pathogens treated with IPL and UVC (254 nm) decreased exponentially with treatment time. Particularly dramatic reductions in L monocytogenes and E. coli O157:H7 were observed for IPL treatments at energy densities of 376 and 455 W/m(2), with an approximately 7-log reduction for a treatment time of 60-180 s. Also, a 4-fog reduction of L monocytogenes and a 5-log reduction of E. coli O157:H7 were achieved with UVC irradiation for 1200 s. The types and amounts of IPL- and UVC-induced DNA damage in both microorganisms were determined and compared. DNAs from cells irradiated with either IPL or UVC accumulated double-strand breaks (DSBs), single-strand breaks, and cyclobutane pyrimidine dimers, and with a similar pattern: however, more DSBs were detected following UVC than following IPL in both types of microorganism. Transmission electron microscopy observations of IPL- and UVC-induced cell damage clearly indicate that bacterial cell structures were destroyed by IPL treatment but not by UVC treatment. (C) 2011 Elsevier Ltd. All rights reserved.
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