Journal
FOOD CONTROL
Volume 24, Issue 1-2, Pages 72-77Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2011.09.005
Keywords
One-step; Test strip; Nanoparticle-McAb probe; Fumonisin
Categories
Funding
- National High-tech R&D Program of China (863 Program) [2007AA10Z426, 2007AA10Z440]
- National Natural Science Foundation of China [30771657, 60971011]
- Young Man Priming Foundation of Jilin University [4305050102E8]
Ask authors/readers for more resources
Fumonisins (FBs), possible carcinogen to humans, are known to occur as a natural contaminant of corn worldwide. A monoclonal antibody (McAb) against FBI, which has high cross reactivity with FB2 and FB3 was produced and a nanoparticle-McAb probe was synthesized. Based on the probe, the one-step competitive immunochromatographic assay test strip for the rapid detection of total FBs (FBI, FB2 and FB3) was developed and applied to maize samples. The colour density of the test line is proportional to FBs mixture (FB1:FB2:FB3, 12:4:1) concentration in the range 2.5-40 ng mL(-1). The visual detection limit of FBs mixture spiked maize samples was found to be 2.5 ng mL(-1). The qualitative test based on the visual evaluation of results for FBs detection can be completed in 10 min. The performance of the assay is easy and convenient without the need of any instrumentation. The results demonstrated that the gold-McAb probe based strip could be used as a qualitative tool for rapid screening technique of FBs on-site. (C) 2011 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available