Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 55, Issue -, Pages 297-303Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2012.12.039
Keywords
4,5-O-Dicaffeoylquinic acid methyl ester; Trans-caffeic acid; Human intestinal flora; Biotransformation; Nitric oxide; Antioxidant activity
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4,5-O-Dicaffeoylquinic acid methyl ester (1) was anaerobically incubated with human intestinal flora and four biotransformation products (2-5) were obtained. Their structures were elucidated as 3,4-O-dicaffeoylquinic acid methyl ester (2), 3-hydroxyphenylpropionic acid (3), trans-caffeic acid (4) and 3,4-dihydroxyphenylpropionic acid (5) on the basis of their spectroscopic data. Using high-performance liquid chromatography equipped with a diode array detector, chromatographic separation of 1-5 was performed on an analytical C-18 column. The time course of the biotransformation was studied to probe into the biotransformation mechanism of 1 by human intestinal flora. In addition, the inhibitory activity of the parent compound 1 and its four main biotransformation products 2-5 on the inhibition of nitric oxide production induced by lipopolysaccharide in macrophage cell line RAW 264.7 and their DPPH free radical-scavenging activity in cell-free bioassay system were estimated. (C) 2012 Elsevier Ltd. All rights reserved.
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