Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 49, Issue 10, Pages 2600-2608Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2011.07.004
Keywords
Juniperus drupacea; HPLC-DAD-ESI-MS; Phenolic compounds; Antioxidant activity; Antimicrobial activity; Cytotoxic activity
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Funding
- Federfarma Messina
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The present study was designed to define the phenolic profile and the biological potential of berries methanol extract of Juniperus drupacea Labill. from Turkey. The total phenolic content (Folin-Ciocalteau assay) was 48.06 +/- 0.99 mg GAE/g extract. The HPLC-DAD-ESI-MS analysis allowed the determination of the complete phenolic profile of J. drupacea berries. Phenolic acids represented more than 60% of the total phenolics, and tyrosol was the major one (1324 +/- 0.64 mu g/g extract); within the flavonoids amentoflavone was detected as the main constituent (927 +/- 0.35 mu g/g extract). The extract exhibited good antioxidant properties, as determined by different in vitro models: DPPH test (IC50 0.38 +/- 0.02 mg/mL), reducing power (12.63 +/- 0.14 ASE/mL), Fe2+ chelating ability (IC50 2.26 +/- 0.06 mg/mL), and TBA test (IC50 2.47 +/- 1.13 mu g/mL). Cytotoxicity against Artemia salina was highlighted (LC50 489.47 +/- 27.8 mu g/mL), and a significant decrease (p <= 0.05; p <= 0.01) in HepG2 cells viability was observed at the higher concentrations (5-10 mu g/mL). The extract displayed good antibacterial activity towards Gram-positive bacteria and in particular Staphylococcus aureus was the most susceptible strain (MIC 78.12 mu g/mL). (C) 2011 Elsevier Ltd. All rights reserved.
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