Article
Chemistry, Physical
L. Quintana-Gomez, M. Ladero, L. Calvo
Summary: The synthesis of fatty acid methyl ester (FAMEs) biodiesel from alperujo oil using Novozym 435 and Lipozyme TL IM as catalyst in scCO2 showed improved conversion of triglycerides at higher temperatures and longer reaction times, while also decreasing residual enzyme activity.
JOURNAL OF SUPERCRITICAL FLUIDS
(2021)
Article
Chemistry, Multidisciplinary
Anamaria Todea, Sara Fortuna, Cynthia Ebert, Fioretta Asaro, Stefano Tomada, Marco Cespugli, Fabio Hollan, Lucia Gardossi
Summary: The lipase-catalyzed polycondensation of azelaic acid and glycerol was investigated using a Design-of-Experiment approach. The study provided rational guidelines for optimizing the reaction and successfully scaled it up for industrial use.
Article
Chemistry, Physical
Bruno Rodrigues Wenceslau, Katia Andressa Santos, Edson Antonio da Silva, Lucio Cardozo-Filho, Camila da Silva, Rogerio Favareto
Summary: The study investigated oil extraction from Syagrus oleracea kernels using supercritical CO2 and compressed propane. It found that compressed propane extraction had lower pressure and solvent consumption, and was advantageous for the recovery of beta-sitosterol.
JOURNAL OF SUPERCRITICAL FLUIDS
(2021)
Review
Biotechnology & Applied Microbiology
Siti Hajar Hamdan, Jonathan Maiangwa, Mohd Shukuri Mohamad Ali, Yahaya M. Normi, Suriana Sabri, Thean Chor Leow
Summary: Thermal stability is crucial in the search for novel lipases, with thermophilic microorganisms offering new opportunities for biotechnological applications. Enzyme structure modifications are essential for optimizing stability in thermophilic conditions, particularly in the food industry and biodiesel production. There is a trade-off between enzyme use in organic solvents and product generation, but enhancing stability is key for maintaining enzymatic activity regardless of the environment.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Maja Leitgeb, Zeljko Knez, Gordana Hojnik Podrepsek
Summary: The objectives of this study were to inactivate certain enzymes in flour using supercritical carbon dioxide and optimize the treatment conditions. The study found that exposure to scCO(2) can decrease the activity of unwanted enzymes in flour. In scCO(2)-treated flour, the fat content decreased significantly, the grain structure remained intact, and the resulting bread had a lower specific surface volume.
Article
Chemistry, Physical
Jaime Jaramillo-Gutierrez, J. L. Lopez-Picon, Jose Torres-Arenas
Summary: The Thermodynamic Geometry (TG) of Mie fluids in the subcritical and supercritical region is studied using a third order thermodynamic perturbation theory equation of state (EOS). The R-crossing method of TG is applied to reproduce the coexistence curves related to Mie fluids. The behavior of the R-Widom line is analyzed and found to be dependent on the stiffness and range potential for the Mie fluids. A correspondence states principle is found for the R-Widom line, and a Clausius-Clapeyron-type relation near the critical point in the supercritical region is fulfilled.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Organic
Orlando G. Elso, Augusto E. Bivona, Rocio Cenizo, Emilio L. Malchiodi, Guadalupe Garcia Linares
Summary: In our project on developing new antiparasitic agents, we synthesized a series of amides derived from amlodipine, a calcium channel blocker used as an antihypertensive drug, through enzymatic synthesis. By using lipase-catalyzed acylation, we obtained nineteen amlodipine derivatives, with eighteen of them being new compounds. The synthesized compounds were evaluated for antiproliferative activity against Trypanosoma cruzi, the causative agent of Chagas' disease, and some of them showed significant activity against the clinically relevant form of the parasite. The derivatives of myristic and linolenic acids demonstrated the highest efficacy and lowest cytotoxicity among the synthesized compounds. The enzymatic methodology used in this study offers mild reaction conditions and low environmental impact, making it a valuable approach for synthesizing promising antiparasitic agents based on amlodipine.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Mar Serrano-Arnaldos, M. Dolores Murcia, Salvadora Ortega-Requena, M. Claudia Montiel, Fuensanta Maximo, Elisa Gomez, Josefa Bastida
Summary: The pseudo-first-order kinetic model accurately describes the esterification process of cetyl esters and predicts the reaction rate of spermaceti. The results suggest that lipases can be used as industrial catalysts with the assistance of this simple yet accurate kinetic model.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Daniel A. Grajales-Hernandez, Mariana A. Armendariz-Ruiz, Fernando Lopez Gallego, Juan Carlos Mateos-Diaz
Summary: Alkyl hydroxycinnamates (AHs) are molecules of biotechnological interest with applications in cosmetics, food, and pharmaceuticals. Enzymatic approaches and immobilization techniques play a crucial role in obtaining AHs efficiently.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Fady Abd El-malek, Alexander Steinbuchel
Summary: Biodegradable polymers, such as polyhydroxyalkanoates (PHA), have various industrial and biological uses. However, their processability and mechanical properties are not ideal, limiting their application range. Enzymes and chemicals can be used to modify and improve polymer performance, enhancing stability and production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Jair A. Esquivel Guzman, Hu Zhang, Ernesto Rivera, Marc Lavertu, Xiao-Xia Zhu
Summary: Porphyrins and their derivatives exhibit useful properties in catalysis, photovoltaics, and biomedicine. By synthesizing porphyrin-containing polymers, the applications of porphyrins can be expanded. An enzymatic two-step method involving ring-closure and ring-opening reactions was used to successfully synthesize polyesters with porphyrin moieties, demonstrating good thermal stability and optical properties similar to their precursor porphyrins.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Entomology
Hui-Hui Zhang, Bao-Jun Yang, Yong Wu, Hao-Li Gao, Xu-Min Lin, Jian-Zheng Zou, Ze-Wen Liu
Summary: Lipid metabolism and lipases are crucial for the development and reproduction of insects. In this study, we investigated the activity of triacylglycerol hydrolysis in different tissues of N. lugens and identified 43 lipases. Among them, 23 lipases belonged to neutral lipases and were selected for further experiments. The presence of complete motifs was essential for the activity of neutral lipases in triacylglycerol hydrolysis. Silencing a highly expressed lipase gene in the ovary decreased egg production and hatchability, partially due to a decline in lipid metabolism. Our findings suggest that incomplete motifs in neutral lipases expressed in the intestine may explain the lower lipase activity in this tissue, and ovary-specific neutral lipases are important for N. lugens reproduction.
Article
Biotechnology & Applied Microbiology
Kristian Ray Angelo Are, Shusuke Ohshima, Yusuke Koike, Yoshihisa Asanuma, Shogo Kashikura, Mayumi Tamura, Tomoko Matsuda
Summary: The study achieved carboxylation through enzymatic reaction under specific conditions, resulting in increased yield.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agriculture, Multidisciplinary
Wenyi Chen, Lihong He, Wenzhu Song, Jianrong Huang, Nanjing Zhong
Summary: Most of the encapsulated lipases exhibited moderate and considerable glycerolysis activity. In addition, AOL@GMP/Tb, TLL@GMP/Tb, RML@GMP/Tb, and CALA@GMP/Tb exhibited good reusability in glycerolysis reactions and potential in practical applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Review
Food Science & Technology
Muhammad Talha Afraz, Xindong Xu, Muhammad Adil, Muhammad Faisal Manzoor, Xin-An Zeng, Zhong Han, Rana Muhammad Aadil
Summary: This review article discusses the application of subcritical and supercritical fluid technologies in the valorization of industrial fruit and vegetable waste. It highlights the potential benefits of these advanced extraction techniques for the recovery of bioactive compounds and unconventional oils from waste materials. The article also emphasizes the importance of collaboration between academia, industry, and policymakers to enable the widespread adoption of these promising technologies, contributing to a more sustainable and circular economy.
Review
Biotechnology & Applied Microbiology
Priscila Esteves Faria, Aline M. Castro, Denise Maria Guimaraes Freire, Rafael D. Mesquita
Summary: This article elucidates the details of isomer production of 2,3-butanediol and acetoin, non-oxidative metabolism products of microorganisms, through bioinformatics analysis and compares the stereo-triads of other enzymes. These results are of great importance for understanding the biosynthesis and industrial production of BD/AC.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Engineering, Chemical
Stefanni S. Everton, Isabelly Sousa, Luciana da Silva Dutra, Eliane Pereira Cipolatti, Erika Cristina G. Aguieiras, Evelin Andrade Manoel, Jaqueline Greco-Duarte, Martina C. C. Pinto, Denise Maria Guimaraes Freire, Jose Carlos Pinto
Summary: The research on enzymatic production of biodiesel in Brazil has made progress, with most studies conducted in the southeast region, while biodiesel production is concentrated in the middle western states. The use of biomass as feedstock or raw material for biocatalysts can make the production of biodiesel more sustainable and economically viable.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Review
Agriculture, Multidisciplinary
Renato Dias Matosinhos, Karina Cesca, Bruno Augusto Mattar Carciofi, Debora de Oliveira, Cristiano Jose de Andrade
Summary: This article critically discusses the current status and future trends of mannosylerythritol lipids as green pesticides and biostimulants for seed germination and plant growth. It highlights their potential to partially replace synthetic pesticides, stimulate plant growth, and reduce phytopathogen infections.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Fernanda Raquel Wust Schmitz, Karina Cesca, Alexsandra Valerio, Debora de Oliveira, Dachamir Hotza
Summary: Novel rapid methodologies involving colorimetric biosensors and gold nanoparticles have been developed for the detection of bacteria, specifically Pseudomonas aeruginosa. The biosensor relies on the color change of gold nanoparticles induced by NaCl after incubation with bacteria and aptamer binding. The optimized conditions for detection were found to be 5 μM aptamer concentration and 200 mM of NaCl. The detection limit for P. aeruginosa was 10(5) CFU mL(-1) by the naked eye and 10(4) CFU mL(-1) by UV-Vis spectrometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mirian Cristina Feiten, Iasmin Morigi, Marco Di Luccio, Jose Vladimir Oliveira
Summary: The aim of this study was to investigate the treatment of Candida antarctica Lipase B (CalB) in supercritical carbon dioxide (SC-CO2) and liquefied petroleum gas (LPG), as well as its activity after high-pressure treatment. The results showed that CalB treated with SC-CO2 at 35 ℃, 75 bar for 1 hour and LPG at 65 ℃, 30 bar for 1 hour had residual specific hydrolytic activities of 132% and 142%, respectively. The enzyme treated at high pressure still maintained an activity above 100% after 20 days of storage at low temperatures. There was no significant difference in the residual activity loss of CalB treated with LPG and stored at different temperatures over time. However, a greater difference was observed between CalB treated with CO2 and flash-frozen in liquid nitrogen (-196 ℃) followed by storage in a freezer (-10 ℃) compared to CalB stored in a freezer at -10 ℃. These findings suggest the need for further studies on CalB and other enzymes under different pressurized fluids for industrial applications.
BIOTECHNOLOGY LETTERS
(2023)
Article
Engineering, Chemical
Gabriela N. Pereira, Karina Cesca, Maria Angelica F. Pereira, Carla Roana Monteiro Rudke, Otilia Monica Alves Borges, Anelise Leal Vieira Cubas, Eduardo Zanella, Boris U. Stambuk, Patricia Poletto, Debora de Oliveira
Summary: This study explored the use of non-thermal plasma as an alternative pretreatment technique for different lignocellulosic residues. The results showed that non-thermal plasma can effectively delignify the residues within a short period of time, with minimal impact on the morphology and chemical characteristics of the residues.
JOURNAL OF FOOD PROCESS ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Suzana F. F. Bazoti, Charline Bonatto, Thamarys Scapini, Aline Frumi Camargo, Helen Treichel, Debora de Oliveira
Summary: Over the years, numerous studies have focused on exploring lignocellulosic biomass to achieve high yields of various bioproducts and develop economically viable industrial processes. Levulinic acid (LA) has emerged as a top platform product derived from biomass due to its growing market potential, which attracts investor attention. LA is considered a versatile building block with applications in various industries such as fuels, pharmaceuticals, cosmetics, food additives, and solvents. The primary route for LA synthesis is the acid-catalyzed degradation of cellulose. Sustainable production of LA from lignocellulosic biomass has been extensively researched, along with challenges in the synthesis process and the utilization of residual biomass. This comprehensive review proposes strategies to optimize LA production, such as process intensification and the use of fruit residues.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2023)
Article
Polymer Science
Yago Araujo Vieira, Rosana Oliveira Henriques, Danyelle Gurgel, Diana Hartmann, Ricardo Antonio Francisco Machado, Debora de Oliveira, Bruno Francisco Oechsler, Agenor Furigo Jr
Summary: This study evaluates the potential of polyacrylonitrile particles (PAN) as a support for laccase immobilization. The immobilization yield of laccase was significantly improved using chemically modified PAN particles. The PAN/laccase derivative showed higher activity and stability at low pH and appropriate temperature, indicating the potential of PAN as a new support for laccase immobilization.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Patricia Viera de Oliveira, Otavio Sanaiotto, Ketelin Zamin Kuhn, Amanda Oltramari, Adailton J. Bortoluzzi, Marcelo Lanza, Gean Pablo S. Aguiar, Anna Maria Siebel, Liz Girardi Muller, Vladimir Oliveira
Summary: Microparticles of naringenin were successfully obtained using the gas antisolvent technique, and their potential for treating positive symptoms of schizophrenia was evaluated using a mouse model. The results showed that micronized naringenin exhibited a higher dissolution rate and bioavailability, indicating its potential as an antipsychotic agent.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bruna P. Soares, Ana M. Ferreira, Marina Justi, Luiz Gustavo Goncalves Rodrigues, J. Vladimir Oliveira, Simao P. Pinho, Joao A. P. Coutinho
Summary: Anthocyanins from jucara fruits were extracted using biobased solvents, 1,2-alkanediols and glycerol ethers, through either pressurized liquid extraction (PLE) or ultrasound-assisted extraction (UAE). The optimized PLE conditions resulted in 23.1 mg(anthocyanins)center dot g(dry biomass)(-1), while the UAE conditions with 1,2-propanediol yielded 50 mg(anthocyanins)center dot g(dry biomass)(-1). The biobased solvent solutions exhibited higher anthocyanin yields and antioxidant capacity compared to water and ethanolic extracts.
Article
Environmental Sciences
Ruan F. F. Vieira, Eduardo B. B. Sydney, Karina G. G. Fiametti, Joao H. C. Wancura, J. Vladimir Oliveira
Summary: This study investigated the production of volatile fatty acids (VFAs) by a consortium of microorganisms obtained from whey. The effectiveness of chemical hydrolysis and supplementation with yeast extract and peptone was evaluated to determine the process that provides the highest VFAs production. The results showed that chemical hydrolysis did not significantly affect the production of compounds, while supplementation played a crucial role in increasing VFAs production. Butyric acid was the compound with the highest amount produced, and it had better results with supplemented hydrolyzed whey.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Suzana F. Bazoti, Aline Frumi Camargo, Charline Bonatto, Simone Kubeneck, Helen Treichel, Debora de Oliveira
Summary: This study proposes the synthesis of levulinic acid from watermelon residues, adding value to a residual biomass. The experiments were optimized using acid hydrolysis and a central composite design, with microwave-assisted synthesis showing higher yields. This study contributes to an energy-saving strategy and a circular carbon economy based on agricultural waste.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2023)
Review
Energy & Fuels
Eulalia L. S. Barros, Evertan A. Rebelatto, Diego A. Mayer, Joao H. C. Wancura, J. Vladimir Oliveira
Summary: Sugar esters (SE) are amphiphilic compounds with environmentally friendly features, including non-toxicity, odorless, tasteless, biodegradability, and various functions. The use of lipases as biocatalysts for SE production offers advantages such as reduced energy consumption, higher selectivity, and less use of harmful solvents. Supercritical fluids, especially carbon dioxide, are attractive solvents for SE production due to their unique properties. However, there are challenges in terms of operating conditions and costs. Future research should focus on developing cost-effective bioreactors and enhancing lipases' stability. Rating: 8
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2023)
Article
Biotechnology & Applied Microbiology
Gabriela C. Breda, Priscila E. Faria, Yuri S. Rodrigues, Priscila B. Pinheiro, Maria Clara R. Nucci, Pau Ferrer, Denise M. G. Freire, Rodrigo V. Almeida, Rafael D. Mesquita
Summary: ProspectBIO is a new software for bioprospecting that can efficiently find non-annotated enzyme sequences by searching for homologs in genomes. Using this software, a novel lipase-encoding gene from Ustilago hordei was discovered and successfully expressed in yeast, resulting in an active enzyme.
