Journal
FOOD ANALYTICAL METHODS
Volume 8, Issue 3, Pages 685-695Publisher
SPRINGER
DOI: 10.1007/s12161-014-9943-2
Keywords
Biogenic amines; Fluorescence labeling; Ultrasound-assisted dispersive liquid-liquid microextraction; Foodstuff samples; Box-Behnken design
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Funding
- National Natural Science Foundation of China [31301595, 21275089]
- Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety [GDPKLAPPS1401]
- Qufu Normal University [bsqd 2012017]
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A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.
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