Journal
FOOD ANALYTICAL METHODS
Volume 7, Issue 2, Pages 409-416Publisher
SPRINGER
DOI: 10.1007/s12161-013-9640-6
Keywords
Antioxidant capacity; Optimization; ORAC; Validation; Accuracy profiles; Food matrices
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Funding
- Walloon Ministry
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It is widely accepted that ORAC is a useful method for assessing food extracts that contain various antioxidants. The principal aim of this study was to validate the ORAC assay. We first identified parameters that can interfere with the ORAC assay and we optimized it. Then, experiments were conducted to determine the limits of linearity and response function, to determine the accuracy profiles to circumvent some of the drawbacks of traditional validation procedures. Trueness, selectivity and limits of quantification of the method were also determined. Our objective of ORAC method validation is thus to give guarantees that most of the results generated during use of this method will be close enough to unknown true value of antioxidant capacity of food matrices. The validation results indicate that the described method will give accurate and reliable results for Trolox equivalent values ranging from 50 to 200 mu mol/L.
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