Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Microbiology
Hiroki Tanno, Jean-Marc Chatel, Rebeca Martin, Denis Mariat, Mitsuo Sakamoto, Masao Yamazaki, Seppo Salminen, Miguel Gueimonde, Akihito Endo
Summary: Faecalibacterium prausnitzii is a promising biomarker of a healthy human microbiota, but previous studies lacked specificity and failed to provide information on the heterogeneity within the species. In this study, a new gene marker rpoA was developed to quantify different groups of F. prausnitzii-related taxa, revealing marked differences in abundance and prevalence among the groups in stool samples from healthy adults.
FEMS MICROBIOLOGY ECOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sabrina Flutsch, Fabian Wiestner, Lisa Butticaz, Dominik Moor, Kai N. Stolting
Summary: In this study, a quantitative NGS-based method was developed to accurately measure Vibrio sp. at the limit of quantification through DNA standards and digital PCR. The method represents an advancement in quantitative genomics and supports the development of statistical approaches for measurement uncertainties in NGS. It provides a useful tool for future metagenomic studies aiming to quantify microbial DNA in an absolute manner.
Review
Microbiology
Kyle D. Brumfield, Moiz Usmani, Kristine M. Chen, Mayank Gangwar, Antarpreet S. Jutla, Anwar Huq, Rita R. Colwell
Summary: Vibrio spp. are bacteria that thrive in warm water and moderate salinity, some of which are pathogenic to humans, such as Vibrio cholerae. The global incidence of Vibrio infections is on the rise, closely linked to factors like climate change.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Zhiwei Zheng, Lianwei Ye, Ruichao Li, Sheng Chen
Summary: This study investigated the prevalence and genetic contexts of the bla(CTX-M-14) gene in foodborne Vibrio isolates in China. The results showed that this gene was widely distributed in Vibrio species and could be transferred horizontally between strains, contributing to the rapid increase in prevalence of bla(CTX-M-14)-bearing bacterial pathogens worldwide.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Geosciences, Multidisciplinary
Lars Moeller, Bernd Kreikemeyer, Guennar Gerdts, Gunter Jost, Matthias Labrenz
Summary: This study identified fish, especially in the southern Baltic Sea, as potential winter reservoirs for Vibrio spp., including pathogenic species. The invasive species round goby has the capacity to carry a comparable number of Vibrio spp. cells like water and sediment, making it a potential hot spot for pathogenic Vibrio species.
JOURNAL OF MARINE SYSTEMS
(2021)
Article
Microbiology
Guiomar Denisse Posada-Izquierdo, Antonio Valero, Francisco Noe Arroyo-Lopez, Miriam Gonzalez-Serrano, Alfonso M. Ramos-Benitez, Antonio Benitez-Cabello, Francisco Rodriguez-Gomez, Rufino Jimenez-Diaz, Rosa M. Garcia-Gimeno
Summary: Recent studies have confirmed the presence of Vibrio species in table olive fermentations using molecular biology techniques. Despite no reports of foodborne outbreaks caused by Vibrio from consuming table olives, their role and survival conditions in this environment have yet to be fully understood. Laboratory studies and challenge testing showed that the inoculated Vibrio cocktail did not survive in olive brines during fermentation, but exhibited optimal growth in a laboratory medium at pH 9 and 2% salt. The effect of salt concentration was found to be more significant than pH on the growth parameters, and no growth of the Vibrio cocktail was observed in lye-treated olive fermentations, suggesting the presence of natural antimicrobial compounds in olives.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Igori Balta, Mark Linton, Laurette Pinkerton, Carmel Kelly, Lavinia Stef, Ioan Pet, Ducu Stef, Adriana Criste, Ozan Gundogdu, Nicolae Corcionivoschi
Summary: The increasing antibiotic resistance in Campylobacter species highlights the need for novel antimicrobials that can inhibit virulence without causing resistance or host toxicity. Understanding the antibacterial potency and biological mechanisms of natural antimicrobials is crucial for combating bacterial virulence factors. Further research is necessary to evaluate the effectiveness of plant, animal, bacteria, and marine-derived antimicrobials as promising alternatives to antibiotics.
Article
Microbiology
Wenbin Wang, Jianxin Liu, Shanshan Guo, Lei Liu, Qianyun Yuan, Lei Guo, Saikun Pan
Summary: The study identified 101 outer membrane proteins (OMPs) from V. parahaemolyticus as potential candidates for multivalent vaccines and found 19 OMPs relatively specific to V. parahaemolyticus. Surface proteomic study showed that some OMPs have better availability on the cell surface.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Brady Hirshfeld, Kurtis Lavelle, Katie Yen Lee, Edward Robert Atwill, David Kiang, Bakytzhan Bolkenov, Megan Gaa, Zhirong Li, Alice Yu, Xunde Li, Xiang Yang
Summary: This study investigated the prevalence and distribution of antimicrobial resistance (AMR) in Vibrio spp. and Enterococcus spp. from retail shrimp in California. The results showed that Vibrio spp. and Enterococcus spp. had high levels of resistance to certain antimicrobial drugs. Whole genome sequencing revealed the presence of multiple antimicrobial resistance genes in Vibrio isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Md. Saiful Islam, Anamika Paul, Mithun Talukder, Krishna Roy, Md. Abdus Sobur, Samina Ievy, Md. Mehedi Hasan Nayeem, Saifur Rahman, K. H. M. Nazmul Hussain Nazir, Muhammad Tofazzal Hossain, Md. Tanvir Rahman
Summary: The study identified multi-drug resistant Enterococcus spp., Salmonella spp., and Vibrio spp. from migratory birds, suggesting these birds have the potential to carry and spread antibiotic resistant bacteria, posing potential risks to public health.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Prasanga Madhushani Kumarage, Liyana Arachchilage Dinithi Sandunika De Silva, Gang-Joon Heo
Summary: This article summarizes the antimicrobial resistance properties of Vibrio species isolated from aquatic environments to raise awareness about potential health risks related to Vibrio infections in aquaculture and public health.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Ezhaveni Sathiyamoorthi, Olajide Sunday Faleye, Jin-Hyung Lee, Jintae Lee
Summary: This study investigated the antibacterial, antibiofilm, and antivirulence activities of hydroquinone (HQ) and its derivatives against V. parahaemolyticus and V. harveyi. The compounds 2,3-DMHQ and DBHQ showed significant antibacterial and antibiofilm effects, indicating their potential for controlling V. parahaemolyticus in food production facilities.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Fisheries
Corinne Audemard, Kimberly S. Reece, Robert J. Latour, Lydia M. Bienlien, Ryan B. Carnegie
Summary: This study investigated the factors influencing the wide range of concentrations of human-pathogenic Vibrio bacteria in oysters. The results suggest that oyster genetics and parasitism may affect the presence of Vibrio bacteria. The study highlights the importance of further investigating the influence of oyster genetic background and the multiple interacting factors driving the dynamics of human-pathogenic Vibrio bacteria in oysters.
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Environmental Sciences
Guy Roussel Takuissu, Sebastien Kenmoe, Jean Thierry Ebogo-Belobo, Cyprien Kengne-Nde, Donatien Serge Mbaga, Arnol Bowo-Ngandji, Juliette Laure Ndzie Ondigui, Raoul Kenfack-Momo, Serges Tchatchouang, Josiane Kenfack-Zanguim, Robertine Lontuo Fogang, Elisabeth Zeuko'o Menkem, Ginette Irma Kame-Ngasse, Jeannette Nina Magoudjou-Pekam, Carolina Veneri, Pamela Mancini, Giusy Bonanno Ferraro, Marcello Iaconelli, Lidia Orlandi, Claudia Del Giudice, Elisabetta Suffredini, Giuseppina La Rosa
Summary: This systematic review summarizes the overall prevalence of Hepatitis A virus (HAV) in different water matrices. HAV was found in untreated and treated wastewater, surface water, groundwater, drinking water, and other matrices. The results suggest that water matrices could be an important route of HAV transmission, even in industrialized countries with lower prevalence.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Virology
Loredana Cozzi, Teresa Vicenza, Roberta Battistini, Chiara Masotti, Elisabetta Suffredini, Simona Di Pasquale, Fauconnier Marie-Laure, Carlo Ercolini, Laura Serracca
Summary: The use of natural substances with antiviral properties can reduce foodborne viral diseases. The virucidal effect of Citrus limon and Thymus serpyllum essential oils (EOs) and of Citrus Limon, Thymus serpyllum, and Thymus vulgaris hydrolates on murine norovirus (MNV) was evaluated in this study. The results showed that these natural substances can reduce the viral infectivity, with T. serpyllum EOs and hydrolates causing an immediate reduction of about 2 log and C. limon EOs and hydrolates causing an immediate reduction of about 1.3 log in viral infectivity.
Article
Microbiology
Carmelo Massimo Maida, Fabio Tramuto, Giovanni Maurizio Giammanco, Roberta Palermo, Walter Priano, Simona De Grazia, Giuseppa Purpari, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Mario Palermo, Walter Pollina Addario, Giorgio Graziano, Palmira Immordino, Francesco Vitale, Walter SARI Collaboration Grp, Walter Mazzucco
Summary: Wastewater-based epidemiology is a reliable method for real-time detection and monitoring of enteric pathogens and illegal drugs. In this study conducted in Sicily, a significant correlation was found between the load of SARS-CoV-2 RNA in wastewater and the reported prevalence of COVID-19 cases. The study also identified the impact of SARS-CoV-2 variants and subvariants on the increase in infections. The findings confirm the effectiveness of wastewater monitoring as a powerful tool for surveillance and tracking viral variant spread.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Panagiotis Skandamis, Elisabetta Suffredini, Michael W. Miller, Atle Mysterud, Maria Noremark, Marion Simmons, Michael A. Tranulis, Gabriele Vaccari, Hildegunn Viljugrein, Angel Ortiz-Pelaez, Giuseppe Ru
Summary: The European Commission requested an analysis of the Chronic Wasting Disease (CWD) monitoring programme in multiple European countries. The disease was detected in reindeer, moose, and red deer, with two different phenotypes observed. Finland, Sweden, and other areas of Norway reported the first detections of CWD. The data suggests the need for revised surveillance strategies and criteria for assessing the probability of CWD presence.
Article
Veterinary Sciences
Farzad Beikpour, Francesco Pellegrini, Gianvito Lanave, Michele Camero, Cristiana Catella, Barbara Di Martino, Federica Di Profio, Chiara Masotti, Roberta Battistini, Laura Serracca, Giuseppina La Rosa, Vito Martella, Elisabetta Suffredini
Summary: This study demonstrated the potential of using a commonly used primer set and a nanopore sequencing platform to generate astroviromic data in complex matrices. The study found that astroviruses are widely present in mammals, birds, lower vertebrates, and invertebrates, and are associated with enteric and extra-intestinal diseases. The study also identified avian-origin astrovirus sequences as the predominant type, likely due to contamination of surface water.
VETERINARY SCIENCES
(2023)
Article
Infectious Diseases
Farzad Beikpour, Monica Borghi, Eleonora Scoccia, Teresa Vicenza, Andrea Valiani, Simona Di Pasquale, Silvia Bozza, Barbara Camilloni, Loredana Cozzi, Piero Macellari, Vito Martella, Elisabetta Suffredini, Silvana Farneti
Summary: A survey of hepatitis E virus (HEV) circulation in wild boar in Umbria, Italy during the 2021-2022 hunting seasons revealed a 43.6% prevalence of HEV in liver samples, with viral load ranging from 1.47 to 7.35 log genome copies/g. Age was found to be a factor influencing viral load, with younger animals showing higher viral loads. Sequence analysis identified similarities between wild boar and human HEV strains in Central Italy.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Environmental Sciences
Francesco Triggiano, Osvalda De Giglio, Francesca Apollonio, Silvia Brigida, Fabrizio Fasano, Pamela Mancini, Giusy Bonanno Ferraro, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Luca Lucentini, Nicola Ungaro, Giuseppe Di Vittorio, Onofrio Mongelli, Nelhudoff Albano, Maria Teresa Montagna
Summary: Wastewater monitoring in Italy was used to detect SARS-CoV-2 and its variants during the COVID-19 pandemic. The study evaluated the concentration of the virus in raw wastewater, the correlation with COVID-19 cases, and the impact of temperature and rainfall on viral spread. The findings revealed an inverse relationship between atmospheric temperature and viral load in wastewater, while no correlation was found with precipitation. The predictive model suggested that at least 11 cases/100,000 inhabitants would occur in the 15 days following the detection of the virus in wastewater.
FOOD AND ENVIRONMENTAL VIROLOGY
(2023)
Article
Virology
Gianfranco La Bella, Maria Grazia Basanisi, Gaia Nobili, Rosa Coppola, Annita Maria Damato, Adelia Donatiello, Gilda Occhiochiuso, Antonella Cristina Romano, Mariateresa Toce, Lucia Palazzo, Francesco Pellegrini, Angela Fanelli, Barbara Di Martino, Elisabetta Suffredini, Gianvito Lanave, Vito Martella, Giovanna La Salandra
Summary: Hepatitis E virus (HEV) is widely circulating among wild boars in Southern Italy, with different subtypes identified. Understanding the circulating HEV strains in different geographical areas is crucial for tracking the origin of HEV outbreaks and evaluating the epidemiological role of wild boars as potential virus reservoirs.
Article
Virology
Annalisa Lombardi, Antonia Voli, Andrea Mancusi, Santa Girardi, Yolande Therese Rose Proroga, Biancamaria Pierri, Renato Olivares, Luigi Cossentino, Elisabetta Suffredini, Giuseppina La Rosa, Giovanna Fusco, Antonio Pizzolante, Amalia Porta, Pietro Campiglia, Ida Torre, Francesca Pennino, Alessandra Tosco
Summary: This study monitored the presence of SARS-CoV-2 RNA in raw wastewater samples from six wastewater treatment plants and mollusk samples from eight harvesting sites in Campania, Italy. The results showed a high rate of positive wastewater samples and mollusk samples, indicating the potential use of both sample types as sentinels for detecting SARS-CoV-2 in the environment. The lower positivity rate in mollusks compared to sewages was attributed to the dilution effect of seawater.
Article
Microbiology
Andrea Palombieri, Federica Di Profio, Vittorio Sarchese, Paola Fruci, Elisabetta Suffredini, Vito Martella, Carolina Veneri, Giusy Bonanno Ferraro, Pamela Mancini, Giuseppina La Rosa, Barbara Di Martino
Summary: This study detected the presence of rat hepevirus in sewage samples collected from wastewater treatment plants in Abruzzo, Italy, and found a clear geographic/Wastewater Treatment Plant (WWTP)-related pattern.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Michele Equestre, Cinzia Marcantonio, Nadia Marascio, Federica Centofanti, Antonio Martina, Matteo Simeoni, Elisabetta Suffredini, Giuseppina La Rosa, Giusy Bonanno Ferraro, Pamela Mancini, Carolina Veneri, Giovanni Matera, Angela Quirino, Angela Costantino, Stefania Taffon, Elena Tritarelli, Carmelo Campanella, Giulio Pisani, Roberto Nisini, Enea Spada, Paola Verde, Anna Rita Ciccaglione, Roberto Bruni
Summary: This study investigated SARS-CoV-2 variants circulating among military and civilian personnel at an Air Force airport in Italy from November 2020 to March 2022. The viral isolates were classified and their evolutionary relationships were traced using sequencing methods. Several variants were identified, including B.1.258, B.1.177, Alpha, Gamma, and Delta in 2020 and 2021, and only Omicron and its sub-lineage variants in 2022. Screening of the isolates revealed natural resistance and unusual substitutions in genomic regions. The study highlights the importance of continuous genomic surveillance to monitor the evolution of SARS-CoV-2 in both the general population and restricted communities.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana EFSA Panel Biological Hazards BIOHAZ, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Romolo Nonno, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Juan Evaristo Suarez, Estefania Noriega Fernandez, Frederique Istace, Jaime Aguillera, Rosella Brozzi, Ernesto Liebana, Beatriz Guerra, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach is a generic pre-evaluation method used to assess the safety of microorganisms intended for use in the food or feed chains. This approach involves confirming safety concerns at the species/strain or product level, and assessing qualifications related to acquired antimicrobial resistance (AMR) genes. The presence of AMR genes conferring resistance to clinically relevant antimicrobials is considered a hazard and requires further assessment.
Article
Public, Environmental & Occupational Health
Aparna Keshaviah, Megan B. Diamond, Matthew J. Wade, Samuel V. Scarpino
Summary: We surveyed wastewater monitoring programmes in 43 countries to inform the development of global monitoring systems. Most programmes focused on urban populations. High-income countries primarily used composite sampling at treatment plants, while low-income and middle-income countries often used grab sampling from surface waters, drains, and latrines. Though most programmes analyzed samples domestically, there was a longer processing time in low-income and middle-income countries. While SARS-CoV-2 variant monitoring was common in high-income countries, it was less frequent in low-income and middle-income countries. Most programmes shared data internally, but not publicly. These findings emphasize the importance of integrating and expanding wastewater initiatives for global disease surveillance.
LANCET GLOBAL HEALTH
(2023)
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Alessandra de Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) is a generic pre-assessment method that supports the work of EFSA's Scientific Panels by evaluating the safety of microorganisms in the food or feed chains. The evaluation is based on the body of knowledge and safety concerns of the microorganisms. Some strains, such as filamentous fungi, bacteriophages, oomycetes, and certain bacteria, are excluded from the QPS assessment due to their potentially harmful traits. The QPS list is regularly reviewed and updated, and new TUs can receive QPS status if they meet the necessary qualifications.
