Journal
FOOD ANALYTICAL METHODS
Volume 1, Issue 2, Pages 136-143Publisher
SPRINGER
DOI: 10.1007/s12161-008-9023-6
Keywords
Cashew Nut; Real-time PCR; Validation
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Funding
- Bavarian State Ministry for the Environment, Health and Consumer Protection
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Appropriate detection methods have to be provided to assure the compliance with the recently established regulatory provisions concerning the labeling of allergens in food. Therefore, a novel real-time polymerase chain reaction (PCR) system for the specific and sensitive detection of cashew nut (Anacardium occidentale) was developed. Specificity was checked against DNA from 56 plant and animal species to avoid cross-reactivity to phylogenetically related and other food-relevant organisms. The absolute limit of detection (LOD) was determined to be 0.5 pg genomic cashew DNA and 10 copies, respectively, and the practical LOD examined exemplarily for pesto Genovese was 2 mg/kg. In addition, analysis of different retail samples was performed to demonstrate the suitability of the new assay for manifold applications.
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