Journal
FOLIA MICROBIOLOGICA
Volume 55, Issue 3, Pages 258-264Publisher
SPRINGER
DOI: 10.1007/s12223-010-0038-1
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Funding
- National Basic Research and Development Program of China [2009CB119200]
- National High Technology Research and Development [2006AA10Z432]
- National Key Technologies Research and Development [2006 BAD08A14]
- Special Fund of the Monitoring and Control of Plant Diseases and Insect Pests of the Chinese Ministry of Agriculture [(2130108)]
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Dwarf bunt of wheat, caused by Tilletia controversa Kuhn, is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 mu g of teliospores in a 25-mu L PCR reaction mixture.
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