Article
Cell Biology
Lisa Donker, Ronja Houtekamer, Marjolein Vliem, Francois Sipieter, Helena Canever, Manuel Gomez-Gonzalez, Miquel Bosch-Padros, Willem-Jan Pannekoek, Xavier Trepat, Nicolas Borghi, Martijn Gloerich
Summary: Epithelial cells adapt their rate of cell division by sensing changes in cell number through mechanosensitive E-cadherin adhesions, which control G2/M cell-cycle progression.
Article
Multidisciplinary Sciences
Sahla Syed, Henry Wilky, Joao Raimundo, Bomyi Lim, Amanda A. Amodeo
Summary: The research found that the N/C ratio affects the number of cell divisions and the timing of zygotic genome activation, influencing transcription through multiple modes of action.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Kathryn E. Neville, Tara M. Finegan, Nicholas Lowe, Philip M. Bellomio, Daxiang Na, Dan T. Bergstralh
Summary: In addition to Pins localization, interaction between Pins and Discs large is also required for spindle orientation in some cells.
Article
Biochemistry & Molecular Biology
Zejun Fang, Min Lin, Shenghui Chen, Hong Liu, Minjing Zhu, Yanyan Hu, Shanshan Han, Yizhang Wang, Long Sun, Fengjiao Zhu, Chengfu Xu, Chaoju Gong
Summary: E2F1 promotes the cell cycle of CRC by enhancing spindle assembly through the transactivation of stathmin1 and TACC3. PKA-mediated phosphorylation of stathmin1 at Ser16 is essential for the phosphorylation of TACC3 at Ser558, facilitating the assembly of TACC3/clathrin/alpha-tubulin complexes. Dysregulation of stathmin1 and TACC3 leads to abnormal spindle pole formation, including disorganized and multipolar spindles. Overexpression of wild-type stathmin1 promotes cell proliferation and tumor growth, and high levels of E2F1, stathmin1, or TACC3 are associated with poor survival in CRC patients.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Alfonsa Diaz-Torres, Alicia E. Rosales-Nieves, John R. Pearson, Carmen Santa-Cruz Mateos, Miriam Marin-Menguiano, Owen J. Marshall, Andrea H. Brand, Acaimo Gonzalez-Reyes
Summary: Stem cells reside in specialized microenvironments known as niches, where the extracellular matrix (ECM) plays a crucial role in controlling niche homeostasis and providing physical support and signals. Perlecans, a type of secreted proteoglycan, are found to have structural and signaling functions in stem cell niches. Studies on the Drosophila ovary have shown that Perlecan is essential for niche architecture and maintenance of a normal pool of stem cells.
Article
Clinical Neurology
Hunki Kwon, Katherine G. Walsh, Erin D. Berja, Dara S. Manoach, Uri T. Eden, Mark A. Kramer, Catherine J. Chu
Summary: This study aims to establish normative values for sleep spindles across development by analyzing EEG data from developmentally normal children. The results show that sleep spindle activity varies with age and brain regions, and provide age- and region-specific normative values. These findings can accelerate the discovery and diagnosis of neurodevelopmental disorders by using sleep spindles as a noninvasive biomarker for cognitive function.
Article
Multidisciplinary Sciences
Natalie Vaughan, Nico Scholz, Catherine Lindon, Julien D. F. Licchesi
Summary: The cell cycle is regulated by protein phosphorylation and ubiquitylation. This study reveals a novel potential role for HECTD1 during mitosis, as its depletion leads to decreased cell number, increased proportion of cells with aligned chromosomes, and prolonged mitosis.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Hang Zhao, Lin Shi, Zhengran Li, Ruiyan Kong, Xuejing Ren, Rui Ma, Lemei Jia, Meifang Ma, Shan Lu, Ran Xu, Richard Binari, Jian-Hua Wang, Meng-qiu Dong, Norbert Perrimon, Zhouhua Li
Summary: This study identifies Yun as a factor required for the proliferation of normal and transformed intestinal stem cells in adult Drosophila. Yun acts as a scaffold to stabilize the PHB complex and is regulated by EGFR/MAPK signaling. The Yun/PHB complex interacts with and positively affects the levels of E2F1 to regulate stem cell proliferation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Developmental Biology
Olga Zaytseva, Naomi C. Mitchell, Damien Muckle, Caroline Delandre, Zuqin Nie, Janis K. Werner, John T. Lis, Eduardo Eyras, Ross D. Hannan, David L. Levens, Owen J. Marshall, Leonie M. Quinn
Summary: FUBP1 and Psi, members of the FUSE Binding Protein family, regulate gene transcription by binding to single-stranded DNA and RNA, promoting cell growth and division. Psi activates Myc and stg to promote cell proliferation, while repressing the transcription of tok, a growth inhibitor.
Article
Cell Biology
Anna Frappaolo, Roberto Piergentili, Maria Grazia Giansanti
Summary: Drosophila dividing spermatocytes provide a suitable cell system to study the reorganization of microtubule and actin cytoskeleton systems during cell division. The unique characteristics of Drosophila male meiotic cells make them amenable to investigate the molecular pathways regulating spindle microtubules and cytokinesis, with important implications for cancer and other diseases.
Article
Cell Biology
Diego Sainz de la Maza, Silvana Hof-Michel, Lee Phillimore, Christian Boekel, Marc Amoyel
Summary: This study reveals that the Drosophila testis niche supports germline stem cells and somatic cyst stem cells. While cell-cycle progression is required for cyst stem cell self-renewal, the E2f1/Dp transcription factor coordinates cell cycle progression with stem cell identity by regulating the metabolic state of the cells.
Article
Cell Biology
Rafael Senos Demarco, Brian J. Stack, Alexander M. Tang, Justin Voog, Sharsti L. Sandall, Tony D. Southall, Andrea H. Brand, D. Leanne Jones
Summary: The text discusses the role of Escargot in regulating the maintenance of somatic cyst stem cells in the Drosophila testis by attenuating the activity of the pro-differentiation insulin receptor pathway. The study shows that Escargot positively regulates the expression of an antagonist of insulin signaling and is required to suppress stem cell loss in response to starvation. Understanding how Escargot regulates cell-fate decisions during homeostasis and nutrient availability decline may provide insight into the metabolic regulation of stem cell behavior in other tissues and organisms.
Article
Biochemistry & Molecular Biology
Aiguo Tian, Virginia Morejon, Sarah Kohoutek, Yi-Chun Huang, Wu-Min Deng, Jin Jiang
Summary: In the Drosophila intestine, infection with pathogenic bacteria induces enteroblasts (ISC progenies) to enter the mitotic cycle through upregulation of the epidermal growth factor receptor (EGFR)-Ras signaling pathway. The regenerative ISCs are produced through mitosis of enteroblasts, which do not gain ISC identity before dividing.
Article
Engineering, Environmental
Jiyeon Leem, Seul Kim, Jae-Sung Kim, Jeong Su Oh
Summary: In this study, the effects of exposure to 9,10-phenanthrenequinone (9,10-PQ) were investigated using mouse oocytes as a model system. It was found that 9,10-PQ compromised meiotic maturation by impairing the assembly of the microtubule organizing center and subsequent spindle formation. Additionally, 9,10-PQ exposure inhibited cell cycle progression by disrupting the accumulation of cyclin B1 and the activation of Cdk1. Importantly, the meiotic defects induced by 9,10-PQ were not rescued by decreasing reactive oxygen species (ROS) levels, indicating that 9,10-PQ has ROS-independent activity in regulating cell cycle progression and spindle assembly.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Wanli Yang, Xingyong Chen, Zhengquan Liu, Yutong Zhao, Yufei Chen, Zhaoyu Geng
Summary: The cell cycle plays a crucial role in the regulation of follicular atresia, and sodium outflow and high expression of MMP3 and MMP9 may be responsible for the structural destruction and apoptosis of follicular cells.
