The role of temperature in post-dispersal embryo growth and dormancy break in seeds of Aconitum lycoctonum L.

This research was performed to resolve temperature requirement for embryo growth, dormancy break and seed germination of Aconitum lycoctonum, an Eurasian perennial herb growing in deciduous forests. The dormancy strategy of A. lycoctonum was compared with that of other Ranunculaceae species growing in the temperate deciduous forest habitat. Seeds of A. lycoctonum germinate immediately after embryo growth is completed during winter and seedlings subsequently emerge in early spring. Experiments in controlled conditions revealed that (1) embryo growth and germination only occurred at low temperatures (< 10 degrees C), (2) a high-temperature pre-treatment was not required for germination, and (3) application of gibberellic acid did not overcome the chilling requirement. Based on these results, seeds of A. lycoctonum can be classified as having deep complex morphophysiological dormancy. Dormancy breaking requirements of A. lycoctonum are very similar to related species studied before, suggesting stasis in seed dormancy traits has occurred in the Aconitum-Delphinium clade. (C) 2008 Elsevier GmbH. All rights reserved.