Article
Multidisciplinary Sciences
Satoshi Imamura, Koki Yoshimoto, Shiho Terada, Kaho Takamuro, Ken-ichiro Kamei
Summary: Applying physiological liver temperatures during hepatic differentiation can enhance hepatic functions of hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) and minimize cell damage.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Vemula Harshini, Nitin Shukla, Ishan Raval, Sujit Kumar, Vivek Shrivastava, Amrutlal K. Patel, Chaitanya G. Joshi
Summary: The increasing salinization of freshwater resources due to global warming has raised concerns among freshwater aquaculturists. This study investigated the adaptability of economically important freshwater fish, L. rohita, to varying levels of salinity. The researchers performed RNA-seq analysis on kidney tissue samples from L. rohita maintained at different salinity concentrations and studied differentially expressed genes involved in various pathways. The study identified several genes associated with ion and glucose transportation, as well as pathways related to energy metabolism, calcium ion regulation, immune response, structural reorganization, and apoptosis. The findings provide a better understanding of osmoregulation and adaptation to salinity changes in L. rohita.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
B. S. Yashwanth, Nevil Pinto, A. Sathiyanarayanan, Aparna Chaudhari, Kiran D. Rasal, Mukunda Goswami
Summary: In this study, LRM cell line was developed from muscle tissue of Indian aquaculture species Labeo rohita, and was used for in vitro applications. The LRM cells showed fibroblastic-like morphology and were authenticated by sequencing mitochondrial gene 16S rRNA. This cell line serves as a functional tool for toxicological and biotechnological studies.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Environmental Sciences
Sunil Sharma, Owias Iqbal Dar, Kirpal Singh, Arvinder Kaur, Caterina Faggio
Summary: The study revealed significant effects of Triclosan on biochemical parameters and gene expression in Labeo rohita larvae, with certain biomarkers like glucose, triglycerides, urea, uric acid, and alkaline phosphatase showing significant declines after exposure. Some parameters showed opposite trends during the recovery period, suggesting their importance in identifying the health status of fish under TCS stress.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Mohamed Gatie, Tyler T. Cooper, Reza Khazaee, Gilles A. Lajoie, Gregory M. Kelly
Summary: Metabolism plays a crucial role in embryonic development and cell differentiation. Research has found that XEN cells are sensitive to lactate and utilize lactate accumulation to promote differentiation. These findings are significant for improving the efficiency of cell reprogramming in regenerative medicine.
Article
Fisheries
Nevil Pinto, Mehar Un Nissa, Mujahidkhan A. Pathan, B. S. Yashwanth, M. G. Pratapa, Sanjeeva Srivastava, Mukunda Goswami
Summary: Histopathological and proteomic analysis of liver tissue in fish infected with Edwardsiella tarda revealed changes in tissue structure and protein expression. Differential proteomic analysis identified 257 differentially expressed proteins between the infected and control groups, which were involved in various metabolic, immune, and apoptotic pathways. Targeted proteomic validation further elucidated the pathogenic effects of Edwardsiella tarda infection and the immune response in fish liver.
Article
Multidisciplinary Sciences
Naheed Bano, Amir Nadeem, Sadia Maalik, Sajida Mushtaq, Naeem Iqbal, Ahmad Kamran Khan, Asghar Shabbir, Muhammad Imran, Safia Niaz, Muhammad Yasin, Mirza Abdul Qayyum, Bashir Ahmad, Milan Skalicky, Sulaiman Ali Alharbi, Saleh Alfarraj
Summary: Single cell gel electrophoresis was used to observe the genotoxic effect of bifenthrin and chlorpyrifos on Labeo rohita. The study found that DNA damage increased with time at sub-lethal concentration and slightly decreased after a certain period. The method may be implemented in eco-friendly toxicity evaluation courses.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Environmental Sciences
Sunil Sharma, Owias Iqbal Dar, Kirpal Singh, Sharad Thakur, Anup Kumar Kesavan, Arvinder Kaur
Summary: This study found that Triclosan has significant toxicity to hatchlings of Labeo rohita, inducing alterations in antioxidant/detoxification, metabolic, and neurological genes as well as DNA damage. Principal component analysis identified OTM, TL, TM, TDNA, SOD, and GR as biomarkers for Triclosan toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Medicinal
Sathisaran Umamaheswari, Palanisamy Karthika, Kanagaraj Suvenitha, Krishna Kadirvelu, Mathan Ramesh
Summary: The study revealed that exposure to TPhP led to significant changes in oxidative stress, apoptosis, genotoxicity, and subsequently pathological alterations in tissues of L. rohita fingerlings. The observed effects were dose-dependent, indicating that the toxicity risks of TPhP are associated with exposure concentrations.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Bernardo Oldak, Emilie Wildschutz, Vladyslav Bondarenko, Mehmet-Yunus Comar, Cheng Zhao, Alejandro Aguilera-Castrejon, Shadi Tarazi, Sergey Viukov, Thi Xuan Ai Pham, Shahd Ashouokhi, Dmitry Lokshtanov, Francesco Roncato, Eitan Ariel, Max Rose, Nir Livnat, Tom Shani, Carine Joubran, Roni Cohen, Yoseph Addadi, Muriel Chemla, Merav Kedmi, Hadas Keren-Shaul, Vincent Pasque, Sophie Petropoulos, Fredrik Lanner, Noa Novershtern, Jacob H. Hanna
Summary: The ability to study human post-implantation development is limited. Mouse naive embryonic stem cells have been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured embryo models. This study extends those findings to humans using genetically unmodified human naive embryonic stem cells and successfully recapitulates the organization of post-implantation human embryos.
Article
Fisheries
Mohammad Musharraf, Mukhtar A. Khan
Summary: The optimal dietary copper requirement of fingerling Labeo rohita was determined to be in the range of 4.52-4.75 mg kg(-1) diet through feeding experimental diets with different copper concentrations.
Article
Biotechnology & Applied Microbiology
Vemula Harshini, Nitin Shukla, Ishan Raval, Sujit Kumar, Vivek Shrivastava, Aparna Chaudhari, Amrutlal K. Patel, Chaitanya G. Joshi
Summary: This study comprehensively analyzed the transcriptomic response of Labeo rohita under hypersalinity environment. They identified differentially expressed long non-coding RNAs, microRNAs, and mRNAs, and revealed the regulatory network between these RNAs. The findings showed the upregulation of pathways related to ion transportation, osmolyte production, signal transduction, energy metabolism, and innate immune response.
Article
Fisheries
Shaista Bibi, Rabea Ejaz, Muhammad Amjad Awan, Javeria Arshad, Bushra Allah Rakha, Muhammad Sajjad Ansari, Sana Urooj, Muhammad Zubair Anjum, Shamim Akhter
Summary: The study aimed to determine the physico-chemical characteristics of Labeo rohita milt and find the best extender for its refrigerated preservation. The results showed that diluting the milt in modified Kurokura extender (KE) can increase sperm viability and motility of Labeo rohita.
AQUACULTURE RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Shamna Naseemashahul, Narottam P. Sahu, Parimal Sardar, Femi J. Fawole
Summary: Feeding a vitamin-mineral based nutraceutical conglomerate improved the overall growth of Labeo rohita fingerlings in both stress exposed and non-exposed groups. Hepatic antioxidant genes and enzymes showed significantly higher values in the stress-exposed group, while reduced glutathione levels decreased at the end of the experiment.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
M. G. Hamilton, W. Mekkawy, Md. Badrul Alam, John A. H. Benzie
Summary: The study found that early selection of G1 individuals can enhance genetic gain in harvest weight in a family-based rohu genetic improvement program. This suggests that early visual identification and non-random sampling of larger individuals can be considered in carp genetic improvement programs.
