Article
Multidisciplinary Sciences
Meng Wang, Tingting Ren, Prince Marowa, Haina Du, Zongchang Xu
Summary: This study identified stable reference genes PP2A and TUA5 in S. glauca under different salt concentrations and tissues, providing a reliable method for normalizing gene expression. These reference genes were validated in germinating seeds of S. glauca, highlighting their utility in accurate gene expression profiling.
SCIENTIFIC REPORTS
(2021)
Article
Agronomy
Khalil M. Saad-Allah, Afaf A. Nessem, Mohsen K. H. Ebrahim, Dina Gad
Summary: This study investigated the metabolic and physiological adjustments, as well as gene expression patterns, in five maize genotypes with varying drought tolerance. The results showed that water stress reduced plant growth and yield characteristics, but also triggered adaptive mechanisms such as increased concentration of antioxidants and metabolic activities. The expression of drought-responsive genes was associated with the severity of water stress and varied among genotypes, with G10 and G123 showing the highest drought resistance.
Article
Fisheries
Huadong Yi, Xiaoli Chen, Shuang Liu, Linqiang Han, Jianhui Liang, Yuqing Su, Han Lai, Sheng Bi, Xuange Liu, Yong Zhang, Xiaopin Zhao, Guifeng Li
Summary: The 40-day experiment explored the effects of different salinities on the growth and physiological responses of juvenile largemouth bass. The results suggest that salinities above 10 ppt are not suitable for largemouth bass culture, with 5 ppt being better than 0 ppt.
AQUACULTURE REPORTS
(2021)
Article
Environmental Sciences
Chia-Hao Lin, Po-Ling Yeh, Yu-Chun Wang, Tsung-Han Lee
Summary: This study examined the dynamic regulation of ions and amino acids in the gills and mantle of the Asian hard clam under hyperosmotic conditions, showing significant increases in osmolality, Na+ and Cl- concentrations, as well as specific activities of Na+/K+-ATPase and contents of free amino acids during acclimation to seawater. The study also highlighted the role of taurine, a predominant free amino acid, in the gills and mantles of Asian hard clams under hyperosmotic stress, with taurine transporter expression being significantly and transiently increased in the mantle.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Agronomy
Sabeeha Bashir, Riffat John
Summary: In this study, the response of tomato to chilling stress was evaluated after the application of brassinosteroid (Br) and silicon (Si). The results demonstrated that both Br and Si helped alleviate the growth inhibition, decrease in chlorophyll content, and photosynthetic rate caused by chilling stress. However, Si was found to be more effective in helping tomato tolerate chilling stress than Br.
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Biochemistry & Molecular Biology
Matilde Calderoni, Maddalena Altare, Luca Mastracci, Federica Grillo, Laura Cornara, Aldo Pagano
Summary: This study demonstrates a method for qualitative identification of Peganum harmala seeds using optical and electron microscopy, as well as the development of a real-time qPCR test that can detect Peganum harmala DNA in quantities as low as less than 1 pg. In addition to species specificity and high sensitivity, this method accurately quantifies the presence of Peganum harmala seeds or seed parts in complex herbal mixtures, providing an indication of the danger level of the product.
Article
Environmental Sciences
Shoushuai Feng, Yongkang Qiu, Zhuangzhuang Huang, Yijun Yin, Hailing Zhang, Deqiang Zhu, Yanjun Tong, Hailin Yang
Summary: This study systematically investigated the physiological and metabolic changes of Acidithiobacillus caldus CCTCC M 2018054 under extreme acid stress, revealing that the organism utilizes various strategies like amino acid regulation, membrane fluidity maintenance, and differential gene expression to resist acidic conditions. The upregulation of specific genes like kdpB and the increased levels of certain amino acids play crucial roles in intracellular pH homeostasis and energy supply for combating acid stress, demonstrating the potential application of acidophiles in industrial bioleaching.
ENVIRONMENTAL RESEARCH
(2021)
Article
Microbiology
D. M. Hollenstein, J. Veis, N. Romanov, G. Gerecova, E. Ogris, M. Hartl, G. Ammerer, W. Reiter
Summary: A protein phosphatase PP2A(Rts1) plays a crucial role in the stress response of Saccharomyces cerevisiae, affecting the phosphorylation status of putative substrate proteins and antagonizing Rck2 signaling, which is regulated by the MAPKAP kinase.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Oncology
Aileen I. Fernandez, Niki Gavrielatou, Leena McCann, Saba Shafi, Myrto K. Moutafi, Sandra Martinez-Morilla, Ioannis A. Vathiotis, Thazin Nwe Aung, Vesal Yaghoobi, Yalai Bai, Yvonne G. Chan, Jodi Weidler, Roy Herbst, Michael Bates, David L. Rimm
Summary: This study investigates the use of PD-L1 mRNA level as a predictive biomarker for nonresponse to immune checkpoint inhibitors (ICIs) in lung cancer patients. The results show that high levels of PD-L1 and PD-L2 mRNA are associated with improved long-term benefit and overall survival, while low PD-L1 mRNA level has a high negative predictive value for absence of long-term benefit.
JOURNAL OF THORACIC ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Yushen Jiang, Shanming Zhang, Hong Qin, Shuai Meng, Xuyi Deng, He Lin, Xiaoliang Xin, Yuxin Liang, Bowen Chen, Yan Cui, Yiheng Su, Pei Liang, Guangzhi Zhou, Hongbo Hu
Summary: A quantitative RT-PCR method for detecting the COVID-19 virus was developed in this study, with acceptable performance and reliable evaluation of viral loads with low detection limits.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2021)
Editorial Material
Biochemistry & Molecular Biology
Daniela Stoehr, Markus Rehm
Summary: Researchers demonstrate that hypertonic environments neutralize Mcl-1 by upregulating its antagonist Noxa in HNSCC cells, leading to cell reliance on Bcl-xL for survival and apoptosis upon Bcl-xL inhibition. These findings suggest a common and conserved mechanistic link between hyperosmotic stress and cellular sensitization to apoptosis.
Article
Biochemistry & Molecular Biology
Ameya P. Jalihal, Andreas Schmidt, Guoming Gao, Saffron R. Little, Sethuramasundaram Pitchiaya, Nils G. Walter
Summary: Biological liquid-liquid phase separation is a driving force for the assembly of membraneless organelles. Proteins undergo rapid, reversible condensation in response to environmental fluctuations, with molecular crowding playing an important role. Current methods are emerging to visualize, quantify, and modulate intracellular condensates in live cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Jose Stenio Aragao Reboucas, Fernando Pablo Silva Oliveira, Alan Carvalho de Sousa Araujo, Helena Leao Gouveia, Juliana Machado Latorres, Vilasia Guimaraes Martins, Carlos Prentice Hernandez, Marcelo Borges Tesser
Summary: The global increase in aquatic organism production has resulted in high volumes of waste, which contains bioactive compounds of significant commercial value.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Immunology
Reginaldo G. Bastos, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M. Cooke, Odir A. Dellagostin, Raul G. Barletta, Carlos E. Suarez
Summary: This article discusses the potential use of BCG TI as a strategy to control babesiosis infection more efficiently, supported by experimental evidence and the possibility of vaccine development.
Article
Fisheries
Thamyres Vanessa N. da Silva, Camila F. dos Santos, Jessica M. L. dos Santos, Marcos J. Schmitz, Juan R. B. Ramirez, Marcelo F. Torres, Luis Andre L. Barbas, Luis A. Sampaio, Pablo E. Verde, Marcelo B. Tesser, Jose M. Monserrat
Summary: In this study, the effects of lyophilized Euterpe oleracea as a dietary antioxidant additive were assessed on tambaqui. The results showed that an appropriate level of lyophilized Euterpe oleracea can promote tambaqui growth, enhance skin coloration, improve muscle properties, and increase antioxidant capacity.
AQUACULTURE INTERNATIONAL
(2023)
Article
Chemistry, Applied
Wesclen Vilar Nogueira, Francisco Javier Moyano, Marcelo Borges Tesser, Jaqueline Garda-Buffon
Summary: This study evaluated the potential of peroxidase from soybean meal to mitigate aflatoxin B-1 in fish feed. The results showed that a mitigation efficiency of 90% could be achieved when the enzyme activity was 0.035U/g, temperature was 32 degrees C, time was 6 hours, and humidity was 70%. This suggests that the application of peroxidase in the feed industry could be a promising tool for reducing the risk of aflatoxin contamination in fish farming.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Biology
Ednara Ronise Lima De Araujo, Marcelo Ferreira Torres, Brenda Maria Pereira Alho Da Costa, Moises Hamoy, Luis Andre Sampaio, Luis Andre Luz Barbas
Summary: The aim of this study was to evaluate the level of neuronal depression in juvenile tambaqui fish exposed to plant-derived natural compounds geraniol (GRL) and citronellol (CTL) as anaesthetic alternatives. The results showed that fish exposed to geraniol had an appropriate anaesthetic effect with depression of the central nervous system and gradual recovery of brain activity. In contrast, fish exposed to citronellol exhibited altered electroencephalographic response during induction, which was incompatible with an appropriate anaesthetic effect and smooth recovery.
Correction
Fisheries
Thamyres Vanessa N. da Silva, Camila F. dos Santos, Jessica M. L. dos Santos, Marcos J. Schmitz, Juan R. B. Ramirez, Marcelo F. Torres, Luis Andre L. Barbas, Luis A. Sampaio, Pablo E. Verde, Marcelo B. Tesser, Jose M. Monserrat
AQUACULTURE INTERNATIONAL
(2023)
Article
Microbiology
Raphael R. C. Nascimento, Matheus R. R. Moreno, Raiza S. Azevedo, Jorge A. V. Costa, Luis F. F. Marins, Lucielen O. O. Santos
Summary: This study analyzed the effect of magnetic field (MF) application on the metabolism of Synechococcus elongatus PCC 7942. It was found that MF application significantly increased protein, C-phycocyanin, and allophycocyanin contents in the culture. The biosynthetic route of allophycocyanin was investigated, but there were no statistical differences in gene expression compared to the control culture, suggesting gene induction may occur soon after MF application and stabilize over time. MF application shows promise as a cost-effective method to increase production of commercially valuable compounds by cyanobacteria.
CURRENT MICROBIOLOGY
(2023)
Article
Microbiology
Caroline G. Castro, Marcela G. Meirelles, Raiza Azevedo, Jeferson V. Ramos, Tony L. R. da Silveira, Bruna F. Nornberg, Luis F. Marins
Summary: This study evaluated the effect of the potential probiotic Bacillus subtilis KM0 on the gut microbiota profile and transcription of genes related to intestinal transport in zebrafish. The results showed that exposure to B. subtilis KM0 changed the gut microbiota profile, decreased the number of potentially pathogenic bacteria genera, and downregulated the transcription of transcellular transport genes. Therefore, B. subtilis KM0 exhibited probiotic effects by interfering with pathogenic bacteria proliferation and regulating genes involved in pathogen invasion.
CURRENT MICROBIOLOGY
(2023)
Article
Zoology
Selio Souza da Rocha, Rachel Costa Sabry, Rafael dos Santos Rocha, Rodrigo Maggioni, Bruno Vinicius Silva de Araujo, Renata Julia dos Santos Silva, Joao Marcelo Azevedo de Paula Antunes
Summary: This study detects the presence of P. marinus in oysters for the first time in the estuaries of the Potengi River and the Guarairas lagoon in Brazil using qPCR as a diagnostic tool.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Cell Biology
Maiara Bernardes Marques, Filipe Reis Teodoro Andrade, Estela Fernandes e Silva, Bruno Rodrigues Oliveira, Daniela Volcan Almeida, Ana Paula de Souza Votto, Luis Fernando Marins
Summary: In this study, we found that different chemotherapeutic drugs can selectively choose cells with different antioxidant capacities. Specifically, the selection by daunorubicin (DNR) appears to generate higher energy demands compared to vincristine (VCR). The cells selected by DNR show a greater ability to express major transcription factors related to the antioxidant defense system and the main extrusion pump related to multidrug resistance (MDR) phenotype.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Laura Junqueira de Camargo, Mara Andrade Colares Maia, Rafael dos Santos Woloski, Caroline Rizzi, Gustavo Marcal Schmidt Garcia Moreira, Claus Troger Pich, Luciano da Silva Pinto
Summary: A novel lectin, rBTL, was generated by modifying the amino acid sequence of BanLec. It maintained the structure and specificity of the carbohydrate-binding site, exhibited biological activity, and showed cytotoxicity against human melanoma cells.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Neurosciences
Thiago Angelo Smaniotto, Angela Maria Casaril, Darling de Andrade Lourenco, Fernanda S. Sousa, Fabiana K. Seixas, Tiago Collares, Rafael Woloski, Luciano da Silva Pinto, Diego Alves, Lucielli Savegnago
Summary: Our study investigated the effects of intranasal administration of interleukin-4 (IL-4) on mice with depressive-like behavior induced by chronic unpredictable mild stress (CUMS). We found that IL-4 reversed the depressive behavior and regulated oxidative stress markers in the brain structures, suggesting its potential as an innovative strategy for major depression (MD) treatment.
PSYCHOPHARMACOLOGY
(2023)
Editorial Material
Environmental Sciences
Marcelo O. Soares, Pedro H. C. Pereira, Caroline Feitosa, Rodrigo Maggioni, Rafael S. Rocha, Luis Ernesto Arruda Bezerra, Oscar S. Duarte, Sandra Paiva, Eurico Noleto-Filho, Maiara Queiroz M. Silva, Mayra Csapo-Thomaz, Tatiane M. Garcia, Jose Pedro Vieira Arruda Junior, Kelly Ferreira Cottens, Bruno Vinicius, Ricardo Araujo, Clara Buck do Eirado, Lucas Penna Soares Santos, Tainah Correa Seabra Guimaraes, Carlos Henrique Targino, Jose Amorim-Reis Filho, Wagner Cesar Rosa dos Santos, Alex Garcia Cavalleiro de Macedo Klautau, Livio Moreira de Gurjao, Daniel Accioly Nogueira Machado, Rafaela Camargo Maia, Emanuel Soares Santos, Rachel Sabry, Nils Asp, Pedro B. M. Carneiro, Emanuelle F. Rabelo, Tallita C. L. Tavares, Gislaine Vanessa de Lima, Claudio L. S. Sampaio, Luiz A. Rocha, Carlos E. L. Ferreira, Tommaso Giarrizzo
Summary: This article discusses the recently identified invasion of lionfish in Brazil and proposes a roadmap for urgent mitigation, as well as research and management strategies. The invasion in Brazil is already consolidated, with 352 individuals recorded along the coastline, including juveniles, adults, and egg-bearing females. The lionfish invasion in Brazil has covered a wide range of habitats and regions, indicating a rapid and successful invasion process. Concerns are raised regarding the potential ecological impacts on native species that are vulnerable to lionfish predation. An urgent integrated approach with multiple stakeholders, solution-based ecological research, and a national plan are called for to decrease the impact of lionfish invasion. The experience from the Caribbean and Mediterranean invasions will aid in setting and prioritizing goals for Brazil.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Agriculture, Dairy & Animal Science
Grecica Mariana Colombo, Robson Matheus Marreiro Gomes, Sonia Astrid Munoz Buitrago, Juan Rafael Buitrago Ramirez, Alan Carvalho de Sousa Araujo, Fernando Pablo Silva Oliveira, Virginia Fonseca Pedrosa, Luis Alberto Romano, Marcelo Tesser, Wilson Wasielesky, Jose Maria Monserrat
Summary: The study shows that bioflocs were able to assimilate antioxidants from acai and transfer them to juvenile shrimp. The administration of 20 mg acai L-1 resulted in improved intestinal health and lipid damage mitigation. However, high daily doses of acai (80 mg L-1) led to increased mortality and muscle damage in shrimp.
Article
Biodiversity Conservation
Ueslei S. Leao, Luice G. Bueno, Aline B. Negreiros, Geice R. Silva, Rodrigo Maggioni, Fabio B. Britto, Jose L. R. Sarmento, Diego B. Galvani, Fabio M. Diniz
Summary: The first set of SSR markers for Urochloa mosambicensis is described, derived from a genomic library with 3491 contigs obtained by high-throughput sequencing. Primers were designed for twenty loci, successfully amplified in 39 genotypes, with an average of 4.95 alleles per locus. The markers showed potential transferability to other Urochloa species and could be used for fingerprinting and characterizing genetic diversity.
CONSERVATION GENETICS RESOURCES
(2023)
