Article
Agriculture, Dairy & Animal Science
Paul K. Hershberger, Theodore R. Meyers, Jacob L. Gregg, Maya L. Groner, Sophie A. Hall, Hiruni T. Jayasekera, Ashley H. MacKenzie, Abigail S. Neat, Ella N. Piatt, Kyle A. Garver
Summary: Pathogen surveillances in wild marine fish populations should be carefully designed to address specific research or management objectives. In Pacific herring populations, the potential impacts of viral hemorrhagic septicemia may be underestimated, with disease hot spots recurring annually in certain locations and times.
Article
Fisheries
Najib Abdellaoui, Do-Eun Kim, Yoon Hwan Jeon, Min Sun Kim
Summary: This study investigated the antiviral role of miR-155 in EPC cells infected with VHSV. The results showed that the cells transfected with miR-155 mimic did not exhibit CPE formation or increase in viral titers after VHSV infection, while the expression of immune-related genes Mx1 and ISG15 was upregulated. Therefore, miR-155 may possess antiviral effects against VHSV.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Eun Jin Baek, Min Jae Kim, Kwang Il Kim
Summary: This study evaluated the antiviral activity of three compounds against VHSV infection in olive flounder. The results showed that the compounds significantly suppressed the expression of VHSV genes and maintained cell morphology and viability. Treatment with the compounds improved survival rate and reduced viral shedding in VHSV-infected fish, indicating their potential as antiviral agents.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yin Weili, Huang Wei, Liu Xiaojing, Ni Yangfan, Yang Bai, Shi Qing, Zhao Weiduo, Sun Tao
Summary: The newly developed recombinase polymerase amplification (RPA) assay for the rapid detection of viral hemorrhagic septicemia virus (VHSV) is fast, simple, specific, and sensitive, with a detection limit of 8.3 copies/μL. It has been successfully validated on a large number of fish samples, showing consistent results with traditional RT-PCR and meeting requirements for aquatic animal disease detection.
Article
Fisheries
Jun-Young Song, Shin-Ichi Kitamura, Myung-Joo Oh, Kei Nakayama
Summary: The combined treatment of heavy oil exposure and virus infection in Japanese flounder led to increased mortality. Heavy oil exposure may promote virus replication, leading to higher mortality in the co-treated group. Gene expression profiling showed lower expression of antiviral activity-related genes in the co-treated group. These results help explain the high virus titers in fish treated with both stressors.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Sujin Lee, Ahran Kim, Dahye Yoon, Seonghye Kim, Seohee Ma, Wonho Lee, Do-Hyung Kim, Heui-Soo Kim, Hee-Jae Cha, Yung Hyun Choi, Suhkmann Kim
Summary: This study investigated the metabolic responses in rock bream infected with RBIV using HR-MAS NMR spectroscopy, revealing significant metabolic pathways related to energy metabolism in the liver of heavily infected fish. Lightly infected fish may have survived from heavy infection after 3 weeks. RBIV induced rapid and high energy production in the spleen, while disturbances in metabolism related to the immunological response were observed in the kidney.
Article
Biotechnology & Applied Microbiology
Jun Soung Kwak, Ki Hong Kim
Summary: The study showed that attenuating virus growth can be achieved by inserting specific amiR sequences into the viral genome, and enhancing replication of attenuated virus can be achieved by knocking out the Dicer gene in host cells. This suggests that regulating both viral and host cell genes can impact the virus infectivity.
MARINE BIOTECHNOLOGY
(2021)
Article
Fisheries
Ghazal Moattari, Zahra Izadi, Mostafa Shakhsi-Niaei
Summary: An electrochemical DNA genosensor was fabricated for detecting the VHSV Glycoprotein gene, showing high sensitivity and fast detection. Characterization of the modified electrode and analysis of real samples confirmed the genosensor's acceptable selectivity, stability, regeneration, and reproducibility.
Article
Genetics & Heredity
Kiran Nigar, Sehrish Kakakhel, Asifullah Khan, Hizbullah Khan, Komal Zaib, Shaoqing Wen
Summary: This study aimed to infer the genetic diversity and evolutionary dynamics of global Viral hemorrhagic septicemia virus isolates based on G-gene sequences. The results revealed that the virus is stratified into five genetically distinct subpopulations, and the genotype Ia samples may be stratified into subpopulations based on spatiotemporal distribution.
FRONTIERS IN GENETICS
(2022)
Article
Fisheries
Dinakaran Thirumalai, Woo Ryung Kim, Hyeyoung Joo, Thenmozhi Rajarathinam, Eun Gyung Park, Hyun-jong Paik, Jaewon Lee, Heui-Soo Kim, Seung-Cheol Chang
Summary: We developed a miRNA biosensor for early detection of viral infections in fish farms, specifically for VHSV infection. The biosensor showed a wide linear response range and low detection limit, providing an effective tool for monitoring and preventing the spread of infections in aquaculture.
Article
Agriculture, Dairy & Animal Science
Na-Gyeong Ryu, Eun-Jin Baek, Min-Jae Kim, Kwang-Il Kim
Summary: The iron flocculation method was used to concentrate viruses in water, and the suitability of oxalic and ascorbic acid re-suspension buffers was evaluated. It was found that the oxalic acid buffer was more effective in preserving viral infectivity than the ascorbic acid buffer. This study suggests that the iron flocculation method using an oxalic acid buffer can be used to evaluate viral transmission and predict disease occurrence in the environment.
Article
Fisheries
Se-Young Cho, Seok Ryel Kim, Bipin Vaidya, Joseph Kwon, Duwoon Kim
Summary: This study highlights that defective GLUT4 translocation-dependent glucose uptake increases the mortality of VHSV-infected olive flounders by inhibiting MPO activity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yo-Seb Jang, Soo-Jin Kim, Su-Young Yoon, Rahul Krishnan, Myung-Joo Oh
Summary: This study aimed to investigate the optimal rearing period required for immunizing olive flounder against viral hemorrhagic septicemia virus (VHSV) at suboptimal temperatures. Juvenile olive flounder reared for 5 days at 17 degrees Celsius exhibited 100% survival rate against VHSV infection, while those reared for 30 days showed delayed virus gene expression. These findings provide valuable insights into the immune response of fish to VHSV under suboptimal temperature conditions.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Yo-Seb Jang, Soo-Jin Kim, Su-Young Yoon, Rahul Krishnan, Myung-Joo Oh
Summary: This study demonstrated the effectiveness of a 10-day live VHSV vaccine immunization period at low temperature (17 degrees C). Viral mRNA genes were detected in vaccinated juvenile olive flounder, with lower copy numbers in the internal organs compared to non-vaccinated flounder.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Kyoung-Hui Kong, Ha-Na Jeong, K. U. Shyam, Myung-Joo Oh, Choon-Sup Kim, Hyoung Jun Kim, Wi-Sik Kim
Summary: The LFIA technology developed with seven anti-VHSV antibodies allows for rapid and specific detection of VHSV in olive flounders, with a high sensitivity and specificity. The field validation of the assay showed a diagnostic effectiveness of 97.3% with a specificity of 100% and sensitivity of 93.9%.
Article
Fisheries
Rahul Krishnan, Yo-Seb Jang, Myung-Joo Oh
Summary: In this study, the researchers investigated the effect of beta-glucan on the immune response against nervous necrosis virus (NNV). They found that beta-glucan enhanced the macrophages' ability to combat NNV infection and increased survival rates in infected fish. The findings suggest that beta-glucan has potential applications in vaccination strategies.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jun-Young Song, Shin-Ichi Kitamura, Myung-Joo Oh, Kei Nakayama
Summary: The combined treatment of heavy oil exposure and virus infection in Japanese flounder led to increased mortality. Heavy oil exposure may promote virus replication, leading to higher mortality in the co-treated group. Gene expression profiling showed lower expression of antiviral activity-related genes in the co-treated group. These results help explain the high virus titers in fish treated with both stressors.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Songhak Kim, Bipin Vaidya, Se-Young Cho, Joseph Kwon, Duwoon Kim
Summary: This study successfully replicated HuNoV GII.4 and evaluated virus gene expression in infected zebrafish. The results confirmed the replication of HuNoV in juvenile zebrafish.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Yong Bae Seo, Jiyoung Shin, Ji-Young Yang, Gun-Do Kim
Summary: This study investigated the mitochondrial DNA composition and gene coding of Eopsetta grigorjewi, as well as conducted evolutionary analysis to reveal its close relationship with other species from the same family. The findings are valuable for future research on molecular evolution and the creation of biomarker databases.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Fisheries
Rahul Krishnan, Rahul Rajendran, Yo-Seb Jang, Jong-Oh Kim, Su-Young Yoon, Myung-Joo Oh
Summary: NLRC3, a unique regulatory NLR, is involved in the modulation of cellular processes and inflammatory responses. In this study, the function of NLRC3 in the context of nervous necrosis virus (NNV) infection in seven band grouper was characterized. NLRC3 was found to enhance NNV replication and attenuate interferon responses, while positively regulating inflammasome response and pro-inflammatory gene expression. NLRC3 also negatively regulates the PI3K-mTOR axis and activates the cellular autophagic response. These findings provide important insights into the regulatory mechanism of NLRC3 in the immune response following NNV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Yong Bae Seo, Jiyoung Shin, Ji-Young Yang, Gun-Do Kim
Summary: This study describes the mitochondrial genome structure and gene content of Scorpaena neglecta, providing support for the study of evolution and phylogenetic relationships among Scorpaenidae species.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Yong Bae Seo, Jiyoung Shin, Ji-Young Yang, Gun-Do Kim
Summary: This study analyzed the mitochondrial DNA of Petrale sole Eopsetta jordani and found significant differences with other species in the same family. The results provide valuable data for studying genetic diversity and evolution.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Kyunghoi Kim, Young-Ryun Kim, Seokjin Yoon
Summary: Polyopes affinis, a red alga in the order Halymeniales, has a distinct mitochondrial genome compared to other species in the same order and shows a close relationship with P. lancifolius in phylogenetic analysis.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Review
Fisheries
K. U. Shyam, Hwi-Jin Kim, Sajal Kole, Myung-Joo Oh, Choon-Sup Kim, Do-Hyung Kim, Wi-Sik Kim
Summary: Lateral flow immunoassays (LFIAs) are user-friendly and cost-effective point-of-care detection systems widely used in aquaculture for pathogen detection. This review article summarizes 22 LFIA detection devices used in aquaculture, providing valuable baseline data for the development of new LFIA devices targeting significant pathogens.
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Kyunghoi Kim, Young-Ryun Kim
Summary: This study describes the mitochondrial DNA of Grateloupia elliptica. The circular mtDNA is 28,503 bp in length and encodes 49 genes, including tRNA, rRNA, and protein-coding genes. Phylogenetic analysis reveals that G. elliptica is closely related to G. angusta. The complete mitochondrial sequence of G. elliptica will contribute to the mitochondrial genome database and provide valuable resources for population genetics and evolution analysis.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Yu-Jin Lee, Yong Bae Seo, Jin-Koo Kim, Gun-Do Kim
Summary: The complete mitochondrial genome of Chaetodon modestus was determined for the first time in this study, revealing its gene length, number of protein-coding genes, and base composition. A phylogenetic tree was also constructed to study the relationship within Chaetodontidae.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Environmental Sciences
M. P. Patil, H. -E Woo, I. -C Lee, J. -O. Kim, K. Kim
Summary: In this study, Granulated Coal Ash (GCA) was used as a covering material for sediment remediation. The results showed that GCA can increase the pH and oxidation-reduction potential, and decrease the concentrations of phosphate and hydrogen sulfide in the sediment and overlying water. The microbial community analysis revealed the presence of Proteobacteria, Bacteroidetes, and Firmicutes as the dominant phyla.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Maheshkumar Prakash Patil, Jong-Oh Kim, Yu-Jin Lee, Yong Bae Seo, Jin-Koo Kim, Gun-Do Kim
Summary: This study investigated the mitochondrial genome of the Japanese butterflyfish (Chaetodon nippon). The genome was found to be 16,507 bp in length with 55.4% A + T content, and contained 37 genes. Phylogenetic analysis revealed a close relationship between C. nippon and six other species. These findings provide valuable genetic information for phylogenetic and taxonomic classifications of the Chaetodontidae family.
MITOCHONDRIAL DNA PART B-RESOURCES
(2023)
Article
Fisheries
Maheshkumar Prakash Patil, Jong-Oh Kim, Seung Hyun Yoo, Yong Bae Seo, Yu-Jin Lee, Jin-Koo Kim, Shin-Ichi Kitamura, Gun-Do Kim
Summary: This study provides the first comprehensive mitogenome analysis of Alcichthys elongatus, revealing its genomic features and phylogenetic placement. The mitogenome of A. elongatus is 16,712 bp long, contains thirteen protein-coding genes, twenty-two tRNAs, two rRNAs, and a control region. Phylogenetic analysis based on the protein-coding genes places A. elongatus in the same clade as Icelus spatula. These genomic data are valuable for species identification, phylogenetic analysis, and population genetics.
Article
Entomology
Se-Young Cho, Bipin Vaidya, Hyuk-Jung Kwon, Eunsun Kim, Youngsoon Kim, Ji Yeong Choi, Hyomin Seo, Joseph Kwon, Duwoon Kim
Summary: In this study, proteomic biomarkers and an optimal culture condition for the replication of Oryctes rhinoceros nudivirus (OrNV) were identified using comparative proteomic analysis. Additionally, a machine learning-derived digital imaging analysis was developed to predict OrNV infection in larvae.
ENTOMOLOGICAL RESEARCH
(2023)