Article
Multidisciplinary Sciences
Leah M. Thornton Hampton, Miranda G. Finch, Christopher J. Martyniuk, Barney J. Venables, Marlo K. Sellin Jeffries
Summary: Evidence suggests that early life stage thyroid hormone suppression in fish can have long-term impacts on immune function; exposure to PTU hormone suppressant led to decreased immune cell activity and different transcriptomic responses post infection; laying the foundation for further research on thyroid-immune crosstalk in fish, highlighting potential lasting effects of environmental disruption on immune function in adulthood.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Megumi Matsumoto, Uwe Fischer, Motohiko Sano, Goshi Kato
Summary: The study shows that rainbow trout lacking CD1 can elicit cell-mediated immune responses against Mycobacterioides salmoniphilum mycolic acids, indicating their potential as vaccine candidates to activate CD8α+ T cells.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biology
Pedro Perdiguero, Esther Morel, Carolina Tafalla
Summary: The study utilized the newly developed 10x Genomics single-cell RNA sequencing technology to analyze the transcriptional patterns of individual B cells in the peripheral blood of teleost fish, revealing different subsets and maturation stages. The results offer valuable insights into the biology of teleost B cells and provide potential markers for differentiating trout B cell subsets in the future.
Article
Fisheries
Guro Lokka, Anusha K. S. Dhanasiri, Ashild Krogdahl, Trond M. Kortner
Summary: The increased cultivation of fish and decreased supply of marine ingredients has led to a higher demand for new nutrient sources. This study investigates the effects of adding bile salts, specifically taurocholate, to the culture media of fish gut epithelial cells. The results show that bile salts play important roles in the metabolism, barrier function, and immune response of intestinal epithelial cells.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Henrike Seibel, Ksenia Krassilnikova, Finn-Thorbjorn Fichtner-Grabowski, Alexander Rebl, Carsten Schulz, Stephanie Celine Hornburg
Summary: Substituting fishmeal in fish feed is important for sustainability. This study investigates the effects of a plant-protein based diet on the immune markers in rainbow trout. The results show significant expression differences between diets under handling stress, highlighting the importance of monitoring fish performance under challenging conditions.
AQUACULTURE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Di Wang, Simeng Sun, Shaowu Li, Tongyan Lu, Dongfang Shi
Summary: This study conducted transcriptomic profiling of spleen samples from rainbow trout at 24 h post-Y. ruckeri infection and identified 2498 differentially expressed genes related to immune response pathways. The findings provide insight into the molecular mechanisms of rainbow trout immune responses to Y. ruckeri infection.
Article
Environmental Sciences
Josef Vaclavik, Pavla Sehonova, Jana Blahova, Denisa Medkova, Eva Postulkova, Ondrej Maly, Michaela Charvatova, Kamil Stastny, Jiri Lenz, Jan Mares, Ales Franc, Zdenka Svobodova
Summary: The aim of this study was to investigate the effects of foodborne fluoxetine on juvenile rainbow trout. The results showed significant changes in hematological profile, liver and kidney detoxifying enzyme activity, and damage to the proximal tubules of the caudal kidney, confirming the importance of its impact on immune response.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fabio Sarais, Sophia Kummerow, Ruth Montero, Henrike Rebl, Bernd Koellner, Tom Goldammer, Bertrand Collet, Alexander Rebl
Summary: The study revealed the existence of eight pias genes in the rainbow trout genome, each showing differential expression in tissues and cells. Three structurally different Pias protein variants were found to suppress the activity of the NF-kappa B promoter in a dose-dependent manner, with potential different immune gene regulation in ifn γ-expressing cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Evgeniya V. Pushchina, Anatoly A. Varaksin, Dmitry K. Obukhov
Summary: In this study, the distribution of different cell types and molecular markers in the brain of rainbow trout was investigated. Radial glial cells and neural stem cells were found in the telencephalon and tectum, indicating their importance in adult neurogenesis in rainbow trout.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
F. Docando, N. Nunez-Ortiz, C. R. Serra, P. Arense, P. Enes, A. Oliva-Teles, P. Diaz-Rosales, C. Tafalla
Summary: This study investigates the effects of Bacillus subtilis on the immune response of rainbow trout. The results show that the ABP1 strain has the potential to enhance the immune response of trout and can be used in designing novel oral vaccination strategies for aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Lu Zhao, Jinqiang Huang, Yongjuan Li, Shenji Wu, Yujun Kang
Summary: Rainbow trout is an economically important fish cultivated worldwide, but the outbreak of infectious hematopoietic necrosis virus (IHNV) hampers its farming industry and causes economic losses. This study identified numerous immune-related genes in the skin of rainbow trout infected with IHNV, providing insights into the innate immune system and laying a foundation for further research on disease resistance breeding.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Noeliton Teixeira de Araujo Jr, Patricia Ianella, Tulio Teruo Yoshinaga, Arno Juliano Butzge, Alexandre Rodrigues Caetano
Summary: This study analyzed the diversity and genetic structure of commercially farmed rainbow trout in Brazil using SNP markers. The results showed significant genetic differences between imported strains from Canada and the United States and the other studied groups, and highlighted the importance of proper management practices in avoiding unwanted admixture. Advanced analyzes with low-density SNP data can be used to improve farm management practices.
AQUACULTURE REPORTS
(2023)
Article
Immunology
Diana Martin, M. Camino Ordas, Ines Carvalho, Patricia Diaz-Rosales, Noelia Nunez-Ortiz, Samuel Vicente-Gil, Aitor Arrogante, Carlos Zarza, Marina Machado, Benjamin Costas, Carolina Tafalla
Summary: This study investigates the effects of methionine, tryptophan, and lysine on the functionality of rainbow trout IgM(+) B cells. The results show that methionine supplementation significantly increases cell proliferation and IgM secretion, while tryptophan and lysine have minor or even negative effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Nicole Verdile, Rolando Pasquariello, Gloriana Cardinaletti, Emilio Tibaldi, Tiziana A. L. Brevini, Fulvio Gandolfi
Summary: Aquaculture is expanding due to high demand for fish consumption, prompting efforts to find sustainable alternative feeds and understand intestinal morphology and physiology. Research on intestinal stem-cell niches in fish species and the role of telocytes in intestinal homeostasis has potential to improve sustainability and growth performance in trout farming.
Article
Biochemistry & Molecular Biology
Zhi-Shuai Hou, Meng-Qun Liu, Hai-Shen Wen, Qin-Feng Gao, Zhao Li, Xiao-Dong Yang, Kai-Wen Xiang, Qian Yang, Xin Hu, Meng-Zhi Qian, Ji-Fang Li
Summary: The serotonin system plays a role in regulating physiological functions and behavioral traits in trout. In this study, we identified 39 serotonin receptor genes in rainbow trout and found that some subtypes are associated with immunomodulation and osmoregulation. This study suggests that duplicated trout serotonin receptors may have new physiological functions and potentially have pleiotropic effects in regulating immunomodulation and osmoregulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Nanoscience & Nanotechnology
Nina Jeliazkova, Margarita D. Apostolova, Cristina Andreoli, Flavia Barone, Andrew Barrick, Chiara Battistelli, Cecilia Bossa, Alina Botea-Petcu, Amelie Chatel, Isabella De Angelis, Maria Dusinska, Naouale El Yamani, Daniela Gheorghe, Anna Giusti, Paloma Gomez-Fernandez, Roland Grafstrom, Maciej Gromelski, Nicklas Raun Jacobsen, Vedrin Jeliazkov, Keld Alstrup Jensen, Nikolay Kochev, Pekka Kohonen, Nicolas Manier, Espen Mariussen, Agnieszka Mech, Jose Maria Navas, Vesselina Paskaleva, Aurica Precupas, Tomasz Puzyn, Kirsten Rasmussen, Peter Ritchie, Isabel Rodriguez Llopis, Elise Runden-Pran, Romica Sandu, Neeraj Shandilya, Speranta Tanasescu, Andrea Haase, Penny Nymark
Summary: The proposal of a FAIR-aligned Nanosafety Data Interface aims to improve findability, accessibility, and interoperability of nanosafety data, providing a path towards standardized and optimized use of existing data to promote safe and sustainable nanotechnology.
NATURE NANOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Wilson Corredor-Santamaria, Diego A. Mora-Solarte, Ziv Arbeli, Jose M. Navas, Yohana M. Velasco-Santamaria
Summary: The study utilized Aequidens metae as a sentinel species to investigate the effects of industrial effluents on the Acacias River in Colombia. Results indicated that during the dry season, the highest induction in EROD activity and the most severe histological alterations in fish liver occurred at the discharge site and downstream.
Article
Marine & Freshwater Biology
D. Hernandez-Moreno, M. Blazquez, J. M. Navas, M. L. Fernandez-Cruz
Summary: This study provides new data on the toxicity of biocidal substances and their metabolites to fish, and evaluates the potential use of fish cell lines for assessing acute toxicity. Results show a high positive correlation between in vitro and in vivo data, supporting the use of two fish cell lines and three cytotoxicity endpoints for screening. Overall, fish demonstrate greater sensitivity, but the combined use of cell lines appears to be a valid approach for toxicity assessment.
AQUATIC TOXICOLOGY
(2022)
Article
Environmental Sciences
Judit Kalman, Fernando Torrent, Jose M. Navas
Summary: The study found that in the presence of fetal bovine serum, graphene oxide had a greater impact on the metabolic activity and cell membrane integrity of fish hepatocytes than carbon nanofiber; while in the absence of serum, cell viability in GRM-exposed cells further decreased. Results indicate that primary hepatocytes from rainbow trout are a suitable model to screen for cytotoxicity and to reveal any interaction with GRM.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Polymer Science
David Ibarra, Raquel Martin-Sampedro, Bernd Wicklein, Antonio M. Borrero-Lopez, Concepcion Valencia, Ana Valdehita, Jose M. Navas, Maria E. Eugenio
Summary: The sustainable potential of fast-growing poplar wood as a source for producing cellulose nanofibers (CNFs) was evaluated. The resulting poplar CNFs exhibited properties comparable to eucalypt CNFs, making them a promising alternative. Furthermore, the pH-sensitive hydrogel application of poplar CNFs was identified.
Article
Environmental Sciences
Mona Connolly, David Hernandez-Moreno, Estefania Conde, Alicia Garnica, Jose M. Navas, Fernando Torrent, Isabel Rucandio, Maria L. Fernandez-Cruz
Summary: The study investigated the bioaccumulation potential of TiO2 and CeO2 nanoparticles in fish with different surface coatings. Results showed that the coatings influenced the uptake and distribution of the nanoparticles in fish tissues. Despite differences in depuration kinetics, there was no bioaccumulation potential for both tested metal oxide nanoparticles in fish regardless of coatings according to BMF values < 1.
ENVIRONMENTAL SCIENCES EUROPE
(2022)
Article
Environmental Sciences
Azucena Bermejo-Nogales, Jose A. Rodriguez Martin, Julio Coll, Jose M. Navas
Summary: Rodents are major pests in agriculture and public health. Anticoagulant rodenticides (ARs) are commonly used for rodent control, but increasing resistance is a concern. This study monitored rodent populations in Spain and identified genetic variations that may contribute to resistance. Computational analysis revealed the impact of these variations on the binding affinity of different ARs.
Article
Marine & Freshwater Biology
Elena Badetti, Andrea Brunelli, Eleonora Faraggiana, Judit Kalman, Cinzia Bettiol, Francesca Caterina Izzo, Jose Maria Navas, Antonio Marcomini
Summary: The potential toxicity of manufactured nanoparticles (NPs) and their interaction with chemicals is a growing concern for the environment and human health. This study investigated the combined effects of catecholate type ligands with TiO2 and Fe2O3 NPs in aquatic environments. The results showed that the binding of catechols on the NPs' surface inhibited their cytotoxicity, suggesting that these NPs may reduce the detrimental effects of contaminants.
AQUATIC TOXICOLOGY
(2022)
Article
Environmental Sciences
D. Hernandez-Moreno, J. M. Navas, M. L. Fernandez-Cruz
Summary: In this study, the applicability of the RTgill-W1 cell line as a model to predict the acute and long-term toxicity of nanobiomaterials (NBMs) in fish was assessed. Concentration-dependent effects were observed after long-term exposures to NBMs, with different cytotoxicity mechanisms associated with different NBMs. The RTgill-W1 cell line was found to be a promising model for evaluating the potential toxicity of NBMs in fish.
Article
Environmental Sciences
M. Connolly, G. Moles, F. Candotto Carniel, M. Tretiach, G. Caorsi, E. Flahaut, B. Soula, E. Pinelli, L. Gauthier, F. Mouchet, J. M. Navas
Summary: Tests using algae and/or cyanobacteria, invertebrates (crustaceans) and fish are crucial for assessing the ecotoxicity of substances and classifying them as hazardous or not to the aquatic environment. However, testing two dimensional (2D) graphene nanoforms requires additional attention and adaptations due to their unique attributes. This review critically analyzes existing studies and approaches to testing, providing important insights for future assessments and regulatory requirements.
Article
Medicine, Legal
Eric A. J. Bleeker, Elmer Swart, Hedwig Braakhuis, Maria Luisa Fernandez Cruz, Steffi Friedrichs, Ilse Gosens, Frank Herzberg, Keld Alstrup Jensen, Frank von der Kammer, Jolinde A. B. Kettelarij, Jose Maria Navas, Kirsten Rasmussen, Kathrin Schwirn, Maaike Visser
Summary: This paper provides an overview of the development and adaptation of EU chemicals legislation, guidance, and test guidelines for nanomaterials to promote their safe use. Eleven action needs were identified by a group of experts, covering twenty-two specific information requirements relevant to multiple regulatory areas. These needs focused on resolving issues related to dispersion stability and dosing in toxicity testing, degradation and transformation of organic nanomaterials, and cellular reactivity of nanomaterials. Addressing these issues will lead to better test methods for regulatory compliance and ensure the validity of hazard and risk assessments of nanomaterials. The study emphasizes the importance of a structured process for identifying information needs and generating knowledge, preferably as part of risk governance and connected to technological innovation policy.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Environmental Sciences
W. Corredor-Santamaria, I. C. Calderon-Delgado, Z. Arbeli, J. M. Navas, Y. M. Velasco-Santamaria
Summary: This study evaluated the response of detritivorous fish, Aequidens metae, exposed to polycyclic aromatic hydrocarbons in terms of morphological, biochemical, and genotoxic changes. It found that Aequidens metae is a suitable bioindicator for polycyclic aromatic hydrocarbons monitoring and that phenanthrene exhibits greater genotoxic effects than benzo[a]pyrene.
GLOBAL JOURNAL OF ENVIRONMENTAL SCIENCE AND MANAGEMENT-GJESM
(2023)
Meeting Abstract
Toxicology
A. Rybinska-Fryca, M. Gromelski, M. Vijver, W. J. Peijnenburg, A. Chatel, A. Barrick, N. Manier, J. Kalman, J. M. Navas, K. Jagiello, T. Puzyn
TOXICOLOGY LETTERS
(2021)
Article
Multidisciplinary Sciences
Azucena Bermejo-Nogales, Isabel Rucandio, Mona Connolly, Maria Luisa Fernandez-Cruz, Jose Maria Navas
Summary: The study introduces an easy and cost-effective method for preparing metal oxide nanoparticle-spiked feed to provide the required dietary exposure concentration to fish. By dispersing metal oxide NPs in oil and soaking fish feed pellets, the study achieves stable concentrations of nanomaterials in fish feed for bioaccumulation testing through the dietary route.
Correction
Environmental Sciences
Anna Giusti, Rambabu Atluri, Rositsa Tsekovska, Agnieszka Gajewicz, Margarita D. Apostolova, Chiara L. Battistelli, Eric A. J. Bleeker, Cecilia Bossa, Jacques Bouillard, Maria Dusinska, Paloma Gomez-Fernandez, Roland Grafstrom, Maciej Gromelski, Yordan Handzhiyski, Nicklas Raun Jacobsen, Paula Jantunen, Keld Alstrup Jensen, Agnieszka Mech, Jose Maria Navas, Penny Nymark, Agnes G. Oomen, Tomasz Puzyn, Kirsten Rasmussen, Christian Riebeling, Isabel Rodriguez-Llopis, Stefania Sabella, Juan Riego Sintes, Blanca Suarez-Merino, Speranta Tanasescu, Hakan Wallin, Andrea Haase
