Article
Fisheries
Guo-Juan Niu, Xiao-Xu Zhu, Peng-Yuan Lu, Ming-Chong Yang, Wen-Jie Yuan, Jin-Xing Wang
Summary: This study identified a dyslexia-associated protein, MjKIAA0319L, in shrimps, which recognizes white spot syndrome virus (WSSV) via its extracellular domain and induces endocytosis of WSSV through its intracellular domain. The MjKIAA0319L-AP2-clathrin pathway is involved in the phagocytosis of WSSV to restrict viral infection in shrimps.
Article
Biochemistry & Molecular Biology
Jinbin Zheng, Mengqi Ni, Yong Mao, Yongquan Su
Summary: The study produced and purified recombinant proteins of four CRDs from Marsupenaeus japonicus, demonstrating their varying bacterial binding and antibacterial activities. Additionally, the CRDs showed different capacities to regulate immune effector genes, with His-CRD2-3 displaying broader and stronger inductive effects.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Fisheries
Sen Xu, Ming Jing, De-Min Kong, Ya-Ru Wang, Quan Zhou, Wen-Ying Liu, Fei Jiao, You-Jie Li, Shu-Yang Xie
Summary: The peritrophic membrane serves as a vital physical barrier allowing shrimp to resist pathogen invasion, primarily composed of chitin and proteins. The study identified a chitin-binding protein, MjCBP, in Marsupenaeus japonicus, which was effective in agglutinating bacteria and binding to lipopolysaccharides and peptidoglycans to combat both Gram-positive and Gram-negative bacteria. Dressing MjCBP and CBD1 led to enhanced clearance of V. anguillarum in vivo, demonstrating their role as opsonins or pattern recognition receptors in the shrimp's antibacterial immune response.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Kenji Toyota, Miyuki Mekuchi, Hiroshi Akashi, Shinichi Miyagawa, Tsuyoshi Ohira
Summary: In this study, neuropeptides were identified in the transcriptome of the eyestalk of kuruma prawn. Two sex-determination genes were also identified, one of which showed male-biased expression in the eyestalk ganglion with no sex-specific splicing, unlike insect species. Therefore, this study will advance the research of neuropeptides and sex determination in the kuruma prawn.
Article
Biotechnology & Applied Microbiology
Panpan Wang, Yong Mao, Yongquan Su, Jun Wang
Summary: This study analyzed the codon usage bias, gene expression, and selection pressures of CDSs for two Kuruma shrimp species, Marsupenaeus japonicus and Marsupenaeus pulchricaudatus. The results showed a negative correlation between gene expression level and A/T3s, different optimal codons and usage frequencies of codon pairs for the two species, and the influence of mutations and selection on codon usage patterns. The genetic evolution of these species was mainly driven by mutations and selection pressure.
Article
Biochemistry & Molecular Biology
Xianyun Ren, Xueqiong Bian, Huixin Shao, Shaoting Jia, Zhenxing Yu, Ping Liu, Jian Li, Jitao Li, Alberto Cuesta
Summary: This study identified and characterized the D1 DAR gene in Marsupenaeus japonicus (MjDAD1), showing that its expression was upregulated after low temperature stress. Silencing MjDAD1 resulted in altered levels of various signaling molecules and increased cell apoptosis rate. These findings suggest an important role for MjDAD1 in the environmental adaptation of M. japonicus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Naoaki Tsutsui, Fumihiro Yamane, Makoto Kakinuma, Takao Yoshimatsu
Summary: Molecules related to the insulin signaling pathway are widely conserved in the animal kingdom and play a role in regulating various biological functions. This study identified two new insulin-like peptides in the male gonad of the kuruma prawn, suggesting their involvement in regulating male-specific functions.
Article
Environmental Sciences
Xianyun Ren, Yao Xu, Zhenxing Yu, Cuimin Mu, Ping Liu, Jian Li
Summary: The study revealed the role of Nrf2 in Marsupenaeus japonicas in response to cadmium exposure, with Nrf2 silence leading to increased oxidative stress and apoptosis, while SFN treatment reduced oxidative stress and apoptosis. These findings indicate the significant involvement of the Nrf2 pathway in Cd-induced cellular oxidative damage in M. japonicus.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Jie Zhou, Qiuyu Han, Tomoyuki Koyama, Shoichiro Ishizaki
Summary: After enzymatic hydrolysis, protein hydrolysates from shrimp heads exhibited significant antibacterial and ACE inhibitory activities. Two peptides, VTVP and ARL/I, were successfully isolated from the active fractions. These bioactive peptides have potential applications in the food and pharmaceutical industries.
Article
Fisheries
Xin Zhang, Lefei Jiao, Ming Li, Fangtin Ren, Xinyue Tao, Min Jin, Lu Zhang, Weijian Liu, Qicun Zhou
Summary: Four diets with different carbohydrate-to-protein ratios were used to study the effects on growth performance, antioxidant capacity, and energy metabolism in Marsupenaeus japonicus. The results showed that although increased carbohydrate-to-protein ratios decreased growth performance, there was no significant overall difference. The activity of SOD and GSH decreased significantly with increased carbohydrate-to-protein ratios. The antioxidant and energy metabolism genes showed tissue-specific responses to the dietary ratios.
AQUACULTURE REPORTS
(2023)
Article
Infectious Diseases
Haipeng Cao, Ying Gu, Jing Diao, La Xu, Tianyue Xu, Tongtong Jin, Chunlei Gai
Summary: This study characterized a pathogenic strain of Providencia rettgeri isolated from kuruma shrimp infected with red leg disease. The isolate showed high virulence and multiple antibiotic resistances, and its genome contained potential virulence and antibiotic resistance genes.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Review
Biochemistry & Molecular Biology
Nurul H. Sulimai, Jason Brown, David Lominadze
Summary: This review discusses the role of fibrinogen and its derivatives in inflammatory diseases and their effects on immunomodulation.
Article
Multidisciplinary Sciences
Jirayu Boonyakida, Jian Xu, Jun Satoh, Takafumi Nakanishi, Tohru Mekata, Tatsuya Kato, Enoch Y. Park
Summary: In this study, the truncated VP15 protein VP15((26-57)) showed significant improvement in shrimp survival rate, and the synthesized SR11 peptide also demonstrated enhanced shrimp survival rate. Additionally, the multiligand binding protein MjgC1qR was found to bind with VP15, VP15((26-57)), and SR11.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Heqian Zhang, Jinbin Zheng, Wenzhi Cheng, Yong Mao, Xiangyong Yu
Summary: The anionic peptide MjALF-D displays modest antibacterial activity against certain bacteria and can bind to specific bacteria, providing insights into the function and role of ALF in shrimp immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Shaoqing Zang, Li-Xia Lv, Chen-Fei Liu, Peng Zhang, Cang Li, Jin-Xing Wang
Summary: The mechanisms of trained immunity in shrimp, a phenomenon identified in invertebrates, were investigated in this study. Metabolomic analysis revealed significant changes in metabolism, including enrichment of glycolysis, pentose phosphate pathway, and AMPK signaling pathway, in shrimp trained with UV-inactivated white spot syndrome virus (UV-WSSV). Histone monomethylation and trimethylation were found to be involved in trained immunity. These findings provide insights for WSSV control in shrimp aquaculture.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Xin-Le Kang, Jun-Ying Zhang, Di Wang, Yu-Meng Zhao, Xiao-Lin Han, Jin-Xing Wang, Xiao-Fan Zhao
Article
Fisheries
Li-Jie Huo, Ming-Chong Yang, Jin-Xing Wang, Xiu-Zhen Shi
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Cell Biology
Yu-Qin Di, Xiao-Lin Han, Xin-Le Kang, Di Wang, Cai-Hua Chen, Jin-Xing Wang, Xiao-Fan Zhao
Summary: CTSD has dual functions in promoting cell proliferation and apoptosis, with its secretion determined by N233 glycosylation and expression promoted by 20E hormone. Autophagy triggers its maturation and localization inside cells, leading to activation of CASP3 and apoptosis. Its differential expression in tissues plays a role in determining cell fates during lepidopteran metamorphosis.
Review
Fisheries
Zhe Lin, Jia-Lin Wang, Yang Cheng, Jin-Xing Wang, Zhen Zou
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Editorial Material
Fisheries
Jin-Xing Wang, Gerardo R. Vasta
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Review
Fisheries
Xian-Wei Wang, Gerardo R. Vasta, Jin-Xing Wang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Article
Developmental Biology
Xin-Le Kang, Yan-Xue Li, Yan-Li Li, Jin-Xing Wang, Xiao-Fan Zhao
Summary: Animal steroid hormones can initiate signaling through G protein-coupled receptors. A newly discovered ecdysone-responsive GPCR, ErGPCR-3, plays a crucial role in the lepidopteran insect Helicoverpa armigera. Under 20E induction, ErGPCR-3 forms a homotetramer to facilitate 20E entry into cells, transmitting the 20E signal and promoting metamorphosis.
Article
Immunology
Cang Li, Ming-Chong Yang, Pan-Pan Hong, Xiao-Fan Zhao, Jin-Xing Wang
Summary: The study revealed that after infection with White spot syndrome virus (WSSV), the content of linoleic acid significantly increased in shrimp, suggesting its involvement in the shrimp's antiviral response. Further experiments demonstrated that oral administration of linoleic acid reduced WSSV proliferation in shrimp and increased shrimp survival rate.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Cang Li, Pan-Pan Hong, Ming-Chong Yang, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study identified the involvement of FOXO in systemic and cellular immunity regulation in invertebrates, specifically in shrimp. FOXO plays a critical role in maintaining hemolymph and intestinal microbiota homeostasis by promoting the expression of antimicrobial peptides and enhancing phagocytosis of hemocytes against pathogens. The study revealed the different functions of FOXO in mucosal and systemic antibacterial immunity of invertebrates, providing insights for disease prevention and control in aquaculture.
Article
Genetics & Heredity
Xiao-Pei Wang, Zhen Huang, Yan-Li Li, Ke-Yan Jin, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao
Summary: The regulation of glycometabolism homeostasis is crucial for maintaining health and development. This study used insects as a model and found that the steroid hormone 20-hydroxyecdysone (20E) upregulated the expression of the transcription factor Klf15 to promote autophagy, apoptosis, and gluconeogenesis, thus maintaining glucose homeostasis.
Article
Virology
Peng-Yuan Lu, Guo-Juan Niu, Pan-Pan Hong, Jin-Xing Wang
Summary: In shrimp, the LOXL protein plays a crucial role in antiviral and antibacterial innate immunity by enhancing the expression of antimicrobial peptides.
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study reveals the crucial role of the mTORC1 signaling pathway in the replication of white spot syndrome virus (WSSV) in Marsupenaeus japonicus. WSSV activates calmodulin, leading to the activation of mTORC1 and its downstream effector S6Ks for viral protein translation. This finding suggests that mTORC1 may serve as a potential therapeutic target for controlling WSSV in shrimp aquaculture.
Article
Multidisciplinary Sciences
Xiu-Zhen Shi, Ming-Chong Yang, Xin-Le Kang, Yan-Xue Li, Pan-Pan Hong, Xiao-Fan Zhao, Gerardo R. Vasta, Jin-Xing Wang
Summary: The activation of the IMD pathway, critical for host immunity, is mediated by the receptor SRB2 in crustaceans. Depletion of SRB2 leads to increased bacterial proliferation, shrimp mortality, and decreased expression of antimicrobial peptides. This previously unrecognized SRB2-IMD axis provides insight into the innate immune response in crustaceans and has implications for prevention and treatment strategies against shrimp diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Peng Zhang, Hai-Jing Fu, Li-Xia Lv, Chen-Fei Liu, Chang Han, Xiao-Fan Zhao, Jin-Xing Wang
Summary: AMPK is found to play a significant role in the response of shrimp to white spot syndrome virus (WSSV). The study demonstrates that WSSV infection leads to upregulation of AMPK expression and phosphorylation. The findings suggest that WSSV exploits the host CaMKK-AMPK-mTORC2 pathway for its proliferation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: Previous studies have shown that the mTORC1 signaling pathway plays a role in antiviral functions or benefits viral replication, but the detailed mechanisms by which mTORC1 enhances viral infection are unclear. Our study reveals that WSSV utilizes the mTORC1 pathway for replication in shrimp, suggesting a potential clinical target for controlling WSSV in shrimp aquaculture.
