Article
Fisheries
Xuangang Wang, Xiangfu Kong, Xiaobing Liu, Xubo Wang, Zhigang Wang, Jinxiang Liu, Quanqi Zhang, Haiyang Yu
Summary: Pyroptosis is a caspase-1-dependent form of programmed cell death characterized by cell lysis and inflammatory cytokine release. This study demonstrates that E. tarda infection triggers pyroptosis in macrophages of Japanese flounder, playing a vital role in the immune response. Treatment with a caspase-1 inhibitor significantly reduces pyroptosis, indicating the association of caspase-1 with pyroptosis.
Article
Fisheries
Jun-Young Song, Shin-Ichi Kitamura, Myung-Joo Oh, Kei Nakayama
Summary: The combined treatment of heavy oil exposure and virus infection in Japanese flounder led to increased mortality. Heavy oil exposure may promote virus replication, leading to higher mortality in the co-treated group. Gene expression profiling showed lower expression of antiviral activity-related genes in the co-treated group. These results help explain the high virus titers in fish treated with both stressors.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Hongxiang Wang, Ming Guo, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The study showed that flounder IFN-gamma could act as a promising adjuvant candidate for fish vaccination, significantly enhancing immune responses and protective effects against E. tarda. Co-vaccination with IFN-gamma could boost specific antibody levels and immune-related gene expressions, leading to higher survival rates in vaccinated fish.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Xiaoyan Wu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study demonstrates that the inactivated vaccine against Edwardsiella tarda can induce a protective immune response in flounder, which is mediated through the immediate recognition of the pathogen and subsequent activation of cellular immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Yuanyuan Ding, Shun Zhou, Huimin Guo, Ying Li, Ning Ding, Yunji Xiu
Summary: Galectins are a family of lectins that play crucial roles in the innate immune systems of vertebrates and invertebrates by binding to β-galactoside sugars through their conserved CRDs. This study identified and characterized a PoGRP cDNA from Paralichthys olivaceus, showing it to be widely expressed in various tissues and significantly upregulated in response to bacterial challenge. The recombinant PoGRP protein exhibited binding to various PAMPs and bacteria, suggesting a vital role in protecting against bacterial infection in fish.
Article
Fisheries
Ruoxin Wang, Ping Han, Xiumei Liu, Xubo Wang
Summary: In this study, the role of TRAF genes in the immune response of Japanese flounder was investigated. Six TRAF genes were identified and analyzed, and their expression patterns were studied in different developmental stages and tissues. It was found that some of these genes showed differential expression under temperature stress and pathogen challenge, suggesting their involvement in the immune response. These findings provide insights into the molecular roles of TRAF genes in the regulation of immune and inflammatory responses in Japanese flounder.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Saisai Liu, Haofei Song, Zeyu Liu, Wei Lu, Quanqi Zhang, Jie Cheng
Summary: This study identified suitable reference miRNAs for quantitative expression analysis of miRNAs in Japanese flounder (Paralichthys olivaceus). Through miRNA-Seq analysis and literature search, 10 candidates were selected and their expression stability was evaluated using qRT-PCR and multiple methods. The results showed that miR-22-3p and miR-23a-3p were suitable reference miRNAs for both normal tissues and livers infected with E. tarda in P. olivaceus. These findings provide valuable information for future miRNA expression analysis in P. olivaceus.
Article
Fisheries
Dong-Gyun Kim, Su-Jeong Lee, Jong Min Lee, Eun-Woo Lee, Won Je Jang
Summary: The composition of gut microbiota in flounder changed following infection with pathogenic bacteria. Edwardsiella tarda infection decreased Verrucomicrobia abundance and increased Proteobacteria abundance. Streptococcus iniae infection reduced Verrucomicrobia abundance. Vibrio harveyi infection decreased Firmicutes and Verrucomicrobia abundance and increased Proteobacteria. Infection with all three pathogens increased the relative abundance of Ralstonia and Sphingomonas species and decreased Rubritalea, Saccharimonas, and Bacillus species. Therefore, regulating the gut microbiota composition by reducing Ralstonia and Sphingomonas abundance and increasing Rubritalea, Saccharimonas, and Bacillus abundance might help maintain a healthy gut microbiota balance in flounder. This research could be useful for future studies on improving flounder health through gut microbiota regulation.
Article
Fisheries
Ping Han, Ruoxin Wang, Tingyan Yao, Xiumei Liu, Xubo Wang
Summary: In this study, 13 suppressors of cytokine signaling (SOCS) genes in olive flounder were identified and characterized. The results showed that there were more duplication events in fish than in higher vertebrates, and SOCS genes were highly conserved. The expression profiles and RNA-Seq analysis indicated that SOCS members in olive flounder had specificity in temporality and spatiality and were involved in inflammatory response.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Yunji Xiu, Yingrui Li, Xiaofei Liu, Lin Su, Shun Zhou, Chao Li
Summary: Long non-coding RNAs (lncRNAs) were identified and evaluated for differential expression profiles in the intestine of olive flounder during Edwardsiella tarda infection, providing valuable insights into the regulatory function of lncRNAs in the mucosal immune responses of olive flounders.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Rui Li, Jiangbo Qu, Hengshun Li, Quanqi Zhang
Summary: This study identified 19 scavenger receptor (SRs) family members in Japanese flounder using genome and transcriptome data. Phylogenetic and Ka/Ks analysis revealed five classes within the SRs genes and evidences of purified selection pressures. The study also found that SRs genes are severely conservative within different subfamilies based on gene structure, functional domain, and motif distribution analyses. Additionally, the study demonstrated the important roles of SRs genes in embryogenesis and innate immune response.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
D. S. Liyanage, Sukkyoung Lee, Hyerim Yang, Chaehyeon Lim, W. K. M. Omeka, W. M. Gayashani Sandamalika, H. M. V. Udayantha, Gaeun Kim, Subothini Ganeshalingam, Taehyug Jeong, Seong-Rip Oh, Seung-Hwan Won, Hyoung-Bum Koh, Mun-Kwan Kim, David B. Jones, Cecile Massault, Dean R. Jerry, Jehee Lee
Summary: Selective breeding plays a vital role in the development of disease-resistant traits and high growth rates in flounder aquaculture. This study used a genome-wide association study (GWAS) to analyze viral hemorrhagic septicemia virus (VHSV) resistance in olive flounder. Significant single nucleotide polymorphisms (SNPs) and associated genes were identified, providing valuable information for breeding applications.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Tingyan Yao, Ruoxin Wang, Ping Han, Xiumei Liu, Xubo Wang
Summary: In this study, 11 Toll-like receptor (TLR) genes were identified and classified from the olive flounder genome. Phylogenetic analysis showed high conservation of these genes in the species. Gene structure and motif prediction analysis indicated high sequence similarity among TLRs. Expression patterns in different tissues and developmental stages were spatially and temporally specific. RNA-Seq analysis revealed the involvement of TLR members in inflammatory responses, with two specific genes showing significant differences in response to temperature stress and bacterial infection. This study highlights the important roles of TLR genes in the innate immune response of olive flounder and provides a basis for further functional studies.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyan Wu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: In this study, researchers investigated the protective immune responses induced by an inactivated Edwardsiella tarda vaccine in flounder. The results showed that the vaccine significantly increased the proportions of CD4+ T and CD8+ T lymphocytes, as well as IgM+ B lymphocytes, in the vaccinated group. The vaccine also upregulated the expression of immune-related genes and increased the levels of immune enzymes. Moreover, the vaccine conferred a relative percent survival (RPS) of 54.76%, and reduced the bacterial load in the spleen and head kidney of the vaccinated fish. These findings demonstrate the strong protective efficacy of the vaccine against E. tarda infection in flounder.
MOLECULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xue-Peng Li, Jian Zhang
Summary: This study utilized RNA-Seq technology to investigate the molecular mechanism of fish immune response following TXhfq vaccination, revealing a large number of differentially expressed genes in different tissues, with implications in six immune-related categories. A protein-protein interaction network highlighted the significance of the top six hub genes regulated by TXhfq in immune processes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Fisheries
Tomomasa Matsuyama, Takayuki Minami, Yutaka Fukuda, Natsumi Sano, Takamitsu Sakai, Tomokazu Takano, Chihaya Nakayasu
Article
Fisheries
T. Matsuyama, Y. Fukuda, T. Sakai, N. Tanimoto, M. Nakanishi, Y. Nakamura, T. Takano, C. Nakayasu
JOURNAL OF FISH DISEASES
(2017)
Article
Fisheries
Tomomasa Matsuyama, Yuta Matsuura, Mari Inada, Tomokazu Takano, Chihaya Nakayasu, Takamitsu Sakai, Sachiko Terashima, Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Yasushi Tsuchihashi, Kazushi Odawara, Syunsuke Iwanaga, Tetsuji Masaoka
Article
Immunology
Tomomasa Matsuyama, Natsumi Sano, Tomokazu Takano, Takamitsu Sakai, Motoshige Yasuike, Atushi Fujiwara, Yasuhiko Kawato, Jun Kurita, Kazunori Yoshida, Yukinori Shimada, Chihaya Nakayasu
Article
Multidisciplinary Sciences
Tomokazu Takano, Akatsuki Nawata, Takamitsu Sakai, Tomomasa Matsuyama, Takafumi Ito, Jun Kurita, Sachiko Terashima, Motoshige Yasuike, Yoji Nakamura, Atushi Fujiwara, Akira Kumagai, Chihaya Nakayasu
Article
Multidisciplinary Sciences
Tomomasa Matsuyama, Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Tomokazu Takano, Takeshi Takeuchi, Noriyuki Satoh, Yoshikazu Adachi, Yasushi Tsuchihashi, Hideo Aoki, Kazushi Odawara, Shunsuke Iwanaga, Jun Kurita, Takashi Kamaishi, Chihaya Nakayasu
Article
Immunology
Tomomasa Matsuyama, Yutaka Fukuda, Tomokazu Takano, Takamitsu Sakai, Chihaya Nakayasu
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2018)
Article
Fisheries
Akira Kumagai, Tomokazu Takano, Tomomasa Matsuyama, Takamitsu Sakai, Yuki Nochiri, Ryo Honda, Takumi Kikuta, Miho Honjo
Article
Fisheries
Tomomasa Matsuyama, Tomokazu Takano, Chihaya Nakayasu, Kazushi Odawara, Yasushi Tsuchihashi, Shinji Tanaka, Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Tetsuji Masaoka
Article
Fisheries
Yuta Matsuura, Sachiko Terashima, Tomokazu Takano, Tomomasa Matsuyama
FISH & SHELLFISH IMMUNOLOGY
(2019)
Correction
Multidisciplinary Sciences
Tomomasa Matsuyama, Tomokazu Takano, Issei Nishiki, Atushi Fujiwara, Ikunari Kiryu, Mari Iinada, Takamitsu Sakai, Sachiko Terashima, Yuta Matsuura, Kiyoshi Isowa, Chihaya Nakayasu
SCIENTIFIC REPORTS
(2020)
Article
Fisheries
Yuta Matsuura, Toyohiro Nishioka, Jun Satoh, Yoshiko Shimahara, Tomomasa Matsuyama, Tomokazu Takano, Ikunari Kiryu, Yasuhiko Kawato, Sachiko Terashima, Shukei Masuma, Chihaya Nakayasu
Summary: The market for Pacific bluefin tuna is expanding due to global consumption increases, leading to overfishing and depletion. Aquaculture is being promoted as an alternative, but infectious diseases are a common problem. Research shows that oral infection is effective for reproducing red seabream iridoviral disease in Pacific bluefin tuna.
Article
Fisheries
Tomomasa Matsuyama, Ikunari Kiryu, Mari Inada, Chihaya Nakayasu
Summary: The study revealed that the abalone asfa-like virus (AbALV) causing abalone amyotrophia was present in various tissues of affected abalone, with particularly high quantities in body surface mucus. Both destructive and non-destructive tests were successful in detecting the virus in different tissues and body surface mucus.
Article
Microbiology
Sachiko Terashima, Motoo Suzuki, Tomokazu Takano, Motoshige Yasuike, Tomomasa Matsuyama, Yuta Matsuura, Chihaya Nakayasu
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Microbiology
Tomokazu Takano, Yoji Nakamura, Tomomasa Matsuyama, Takamitsu Sakai, Yuya Shigenobu, Takuma Sugaya, Motoshige Yasuike, Atushi Fujiwara, Hidehiro Kondo, Ikuo Hirono, Yutaka Fukuda, Chihaya Nakayasu
GENOME ANNOUNCEMENTS
(2017)
