Article
Fisheries
Long Zhang, Yanhe Li, Minglin Wu, Haofeng Ouyang, Ruixue Shi
Summary: The study explored the expression pattern of prophenoloxidase (proPO) upon white spot syndrome virus (WSSV) infection in red swamp crayfish, and obtained and analyzed the proPO genomic DNA sequence for the first time. It was found that proPO mRNA levels were significantly upregulated in various tissues after infection, and predicted features in the intron region of proPO were identified. Additionally, three single nucleotide polymorphisms (SNPs) in intron regions were associated with anti-WSSV traits, and recombinant proPO showed potential in reducing WSSV copy numbers and mortality in crayfish.
Article
Fisheries
Lei Zhu, Gaoliang Yuan, Xinyu Jiang, Jie Zhang, Xianliang Zhao, Chao Pei, Li Li, Xianghui Kong
Summary: The Rab5 and Rab6 genes from crayfish Procambarus clarkii were cloned, and their involvement in bacterial and viral infections in the immune response of P. clarkii was revealed. The genes showed wide tissue distribution and increased expression levels after infection with pathogens. Silencing of PcRab5 and PcRab6 affected the mortality rate of crayfish challenged by pathogens, indicating their significant roles in the immune response.
Article
Fisheries
Guihong Fu, Jian Wang, Minglang Cai, Junzhi Zhang, Haibo Hu, Chaozhou Dai, Xin Zheng, Yi Hu, Kaijian Chen
Summary: This study investigated the effects of dietary oleanolic acid (OA) on the growth performance, antioxidant activity, innate immunity, and intestinal microbiota structure of red swamp crayfish. The results showed that OA supplementation improved growth performance, enhanced antioxidant capacity, and did not significantly alter the structure of the intestinal microbiota.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Ying Huang, Ying Jiang, Miao-Miao Wang, Min-Yi Chen, Huan-Gen Chen, Heng-Yuan Chen, Wen-Jie Liu, Xiao-Rui Li, Xiao-Lei Han
Summary: This study cloned and characterized a mannose-binding lectin (MBL) called PcMBL from the crayfish Procambarus clarkii. PcMBL plays important roles in nonself recognition and clearance of invading microorganisms. It was found that PcMBL can influence the transcription of antimicrobial peptide genes and has the ability to bind to various microorganisms and carbohydrates. Furthermore, recombinant PcMBL can facilitate bacterial clearance, inhibit biofilm formation and growth, and reduce mortality in infected crayfish.
AQUACULTURE REPORTS
(2023)
Article
Microbiology
Cheng Chen, Chang-Shuai Liang, Tao Wang, Jing-Lei Shen, Fei Ling, Hai-Feng Jiang, Peng-Fei Li, Gao-Xue Wang
Summary: This study identified rhein as a potent inhibitor of WSSV infection in red swamp crayfish. Rhein inhibited viral transcription, reduced viral load, and improved the survival rate of infected crayfish. It activated JAK/STAT and NF-kappa B pathways, decreased the expression of crustin-1 and BAF, and increased the expression of C-type lectin and proPO to arrest WSSV replication. Additionally, rhein attenuated oxidative and inflammatory stresses and enhanced innate immunity. This research provides great potential for the development of prophylaxis and therapy agents for controlling WSSV infection in crustacean aquaculture.
MICROBIOLOGY SPECTRUM
(2023)
Article
Fisheries
Fanshuang Kong, Yanhong Zhu, Haojie Yu, Xiaoyu Wang, Fatma Ragab Abouel Azm, Junfa Yuan, Qingsong Tan
Summary: The study found that adding an appropriate amount of vitamin C to the diet of red swamp crayfish can improve their growth performance, immunity, and antioxidant capacity. The optimal dietary VC requirement for P. clarkii was estimated to be 265.67 mg/kg diet.
Article
Fisheries
Chen-Yang Lin, Wen-Bin Xu, Bang-Ze Li, Miao-An Shu, Yan-Mei Zhang
Summary: A homolog of cPLA2, PccPLA2, was identified and characterized in red swamp crayfish and was found to play a role in both cellular and humoral immune responses, influencing the expression of antimicrobial peptides and the phagocytic ability of cells.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yan-Mei Zhang, Chen-Yang Lin, Bang-Ze Li, Wei-Ren Dong, Miao-An Shu
Summary: This study identified and characterized Smad3 and Smad4 in the Red Swamp Crayfish. The results indicate their involvement in immune response and important regulatory roles in innate immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xin Ren, Xiaoyu Liu, Xi Zhu, Lijing Xiong, Xufeng Bai
Summary: The presence of trained immunity in red swamp crayfish is important for enhancing their disease resistance and immune capability, which can have significant implications for the crayfish farming industry.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yinan Jiang, Yuting Wang, Yingying Zhang, Hui Yang
Summary: A novel scavenger receptor B (PcSRB) was identified in the Chinese mitten crab, which plays an important role in immune defense, particularly in the recognition and binding of pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Zoology
Yan-Mei Zhang, Chen-Yang Lin, Bang-Ze Li, Wen-Bin Xu, Wei-Ren Dong, Miao-An Shu
Summary: The homologs of FGFR4 were identified from the red swamp crayfish for the first time. pcFGFR4 were highly expressed in muscle and hemocyte and activated in response to bacterial and viral infections, indicating its involvement in the crustacean innate immune responses. Functional analysis showed that pcFGFR4 regulated crayfish's innate immunity by modulating NF-xB signaling pathway.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Fisheries
Xiujuan Zhou, Jing Gong, Yue Zhuang, Fei Zhu
Summary: The study found that coumarin can enhance the immunity of Cherax quadricarinatus, reduce the number of WSSV infections, and delay the infection process. Coumarin also has a positive effect on important immune parameters such as THC, ACP activity, SOD activity, lysozyme activity, and PO activity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Lei Zhu, Xiaoyu Wang, Libo Hou, Xinyu Jiang, Chen Li, Jie Zhang, Chao Pei, Xianliang Zhao, Li Li, Xianghui Kong
Summary: This study investigated the immune response of P. clarkii to A. veronii infection, revealing significant tissue damage and impaired immune system in infected crayfish. Moreover, the activities of immune-related enzymes and the expression levels of immune-related genes were found to be altered in response to A. veronii infection, indicating a comprehensive innate immune response in P. clarkii.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Tianyu Guan, Jianbin Feng, Qianqian Zhu, Long Wang, Peng Xie, Hui Wang, Jiale Li
Summary: This study aimed to explore the defense mechanism of Procambarus clarkii to abamectin. The results showed that abamectin caused oxidative damage to P. clarkii, resulting in damage to the defense system, suppression of nonspecific immunity and antioxidation, and promotion of apoptosis. This study provided a theoretical basis for the healthy cultivation of P. clarkii and further research on the defense mechanism of aquatic animals to pesticides.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Entomology
Zhihao Ming, Zhiqiang Chen, Hao Tong, Xia Zhou, Tingting Feng, Dai Jianfeng
Summary: C-type lectins (CTLs) are a family of proteins that play important roles in innate immune defenses against microorganisms. They bind to a broad range of ligands and mediate immune responses to clear pathogens. Recent studies have shown that CTLs are essential for arthropods' immune responses to arthropod-borne pathogens. This article reviews the current understanding of CTLs derived from important medical arthropods.
Article
Genetics & Heredity
Xin-Le Kang, Jun-Ying Zhang, Di Wang, Yu-Meng Zhao, Xiao-Lin Han, Jin-Xing Wang, Xiao-Fan Zhao
Article
Fisheries
Li-Jie Huo, Ming-Chong Yang, Jin-Xing Wang, Xiu-Zhen Shi
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Cell Biology
Yu-Qin Di, Xiao-Lin Han, Xin-Le Kang, Di Wang, Cai-Hua Chen, Jin-Xing Wang, Xiao-Fan Zhao
Summary: CTSD has dual functions in promoting cell proliferation and apoptosis, with its secretion determined by N233 glycosylation and expression promoted by 20E hormone. Autophagy triggers its maturation and localization inside cells, leading to activation of CASP3 and apoptosis. Its differential expression in tissues plays a role in determining cell fates during lepidopteran metamorphosis.
Review
Fisheries
Zhe Lin, Jia-Lin Wang, Yang Cheng, Jin-Xing Wang, Zhen Zou
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Editorial Material
Fisheries
Jin-Xing Wang, Gerardo R. Vasta
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Review
Fisheries
Xian-Wei Wang, Gerardo R. Vasta, Jin-Xing Wang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Article
Developmental Biology
Xin-Le Kang, Yan-Xue Li, Yan-Li Li, Jin-Xing Wang, Xiao-Fan Zhao
Summary: Animal steroid hormones can initiate signaling through G protein-coupled receptors. A newly discovered ecdysone-responsive GPCR, ErGPCR-3, plays a crucial role in the lepidopteran insect Helicoverpa armigera. Under 20E induction, ErGPCR-3 forms a homotetramer to facilitate 20E entry into cells, transmitting the 20E signal and promoting metamorphosis.
Article
Immunology
Cang Li, Ming-Chong Yang, Pan-Pan Hong, Xiao-Fan Zhao, Jin-Xing Wang
Summary: The study revealed that after infection with White spot syndrome virus (WSSV), the content of linoleic acid significantly increased in shrimp, suggesting its involvement in the shrimp's antiviral response. Further experiments demonstrated that oral administration of linoleic acid reduced WSSV proliferation in shrimp and increased shrimp survival rate.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Cang Li, Pan-Pan Hong, Ming-Chong Yang, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study identified the involvement of FOXO in systemic and cellular immunity regulation in invertebrates, specifically in shrimp. FOXO plays a critical role in maintaining hemolymph and intestinal microbiota homeostasis by promoting the expression of antimicrobial peptides and enhancing phagocytosis of hemocytes against pathogens. The study revealed the different functions of FOXO in mucosal and systemic antibacterial immunity of invertebrates, providing insights for disease prevention and control in aquaculture.
Article
Genetics & Heredity
Xiao-Pei Wang, Zhen Huang, Yan-Li Li, Ke-Yan Jin, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao
Summary: The regulation of glycometabolism homeostasis is crucial for maintaining health and development. This study used insects as a model and found that the steroid hormone 20-hydroxyecdysone (20E) upregulated the expression of the transcription factor Klf15 to promote autophagy, apoptosis, and gluconeogenesis, thus maintaining glucose homeostasis.
Article
Virology
Peng-Yuan Lu, Guo-Juan Niu, Pan-Pan Hong, Jin-Xing Wang
Summary: In shrimp, the LOXL protein plays a crucial role in antiviral and antibacterial innate immunity by enhancing the expression of antimicrobial peptides.
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study reveals the crucial role of the mTORC1 signaling pathway in the replication of white spot syndrome virus (WSSV) in Marsupenaeus japonicus. WSSV activates calmodulin, leading to the activation of mTORC1 and its downstream effector S6Ks for viral protein translation. This finding suggests that mTORC1 may serve as a potential therapeutic target for controlling WSSV in shrimp aquaculture.
Article
Multidisciplinary Sciences
Xiu-Zhen Shi, Ming-Chong Yang, Xin-Le Kang, Yan-Xue Li, Pan-Pan Hong, Xiao-Fan Zhao, Gerardo R. Vasta, Jin-Xing Wang
Summary: The activation of the IMD pathway, critical for host immunity, is mediated by the receptor SRB2 in crustaceans. Depletion of SRB2 leads to increased bacterial proliferation, shrimp mortality, and decreased expression of antimicrobial peptides. This previously unrecognized SRB2-IMD axis provides insight into the innate immune response in crustaceans and has implications for prevention and treatment strategies against shrimp diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Peng Zhang, Hai-Jing Fu, Li-Xia Lv, Chen-Fei Liu, Chang Han, Xiao-Fan Zhao, Jin-Xing Wang
Summary: AMPK is found to play a significant role in the response of shrimp to white spot syndrome virus (WSSV). The study demonstrates that WSSV infection leads to upregulation of AMPK expression and phosphorylation. The findings suggest that WSSV exploits the host CaMKK-AMPK-mTORC2 pathway for its proliferation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: Previous studies have shown that the mTORC1 signaling pathway plays a role in antiviral functions or benefits viral replication, but the detailed mechanisms by which mTORC1 enhances viral infection are unclear. Our study reveals that WSSV utilizes the mTORC1 pathway for replication in shrimp, suggesting a potential clinical target for controlling WSSV in shrimp aquaculture.