Article
Fisheries
Jose Carlos A. Campos-Sanchez, Francisco A. Guardiola, Mara Angeles Esteban
Summary: This study investigated the effects of cantharidin on the inflammatory response in gilthead seabream head-kidney leucocytes (HKLs). The results showed that cantharidin induced apoptosis in HKLs, decreased respiratory burst and phagocytic activities, and increased peroxidase activity. Furthermore, cantharidin also affected the expression of different genes in HKLs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jose Carlos Campos-Sanchez, Francisco A. Guardiola, Maria Angeles Esteban
Summary: This study investigated the effects of lambda-carrageenin on gilthead seabream head-kidney leucocytes (HKLs). The results showed that lambda-carrageenin had no negative effects on cell viability but increased respiratory burst activity and phagocytic ability of HKLs. It also affected the expression of inflammation-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Nora Albaladejo-Riad, Alberto Cuesta, M. Angeles Esteban
Summary: The aim of this study was to induce and characterize extracellular traps (ETs) produced by gilthead seabream (Sparus aurata L.) head-kidney leucocytes. After stimulating the leucocytes with various substances, ETs structures were observed and quantified, revealing different morphological properties depending on the stimulant used. This research provides important insights into the immune response of fish and its potential application in mitigating microbial infections in fish farms.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Rocio Leiva-Rebollo, Dolores Castro, Patricia Moreno, Juan J. Borrego, Alejandro M. Labella
Summary: The study aimed to develop a DNA vaccine for the lymphocystis disease in gilthead seabream. The results showed that the vaccine triggered an immune response and produced specific anti-viral antibodies, providing protection against the disease.
Article
Engineering, Environmental
I. Brandts, C. Barria, M. A. Martins, L. Franco-Martinez, A. Barreto, A. Tvarijonaviciute, L. Tort, M. Oliveira, M. Teles
Summary: This study evaluated the short-term exposure of 45 nm PMMANPs on gilthead seabream by analyzing biomarkers in liver and plasma. The results showed an up-regulation of genes associated with lipid metabolism, an increase in cholesterol and triglycerides in plasma, and activation of antioxidant defenses. After 96 hours, the antioxidant capacity decreased and genotoxicity in blood cells was observed.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Fisheries
Jose Carlos Campos-Sanchez, Javier Mayor-Lafuente, Daniel Gonzalez-Silvera, Francisco A. Guardiola, Maria Angeles Esteban
Summary: The study evaluated the expression of inflammation-related genes in fish skin injected with carrageenin, finding it to be a good stimulator for studying skin inflammation in gilthead seabream. This method could potentially be used to study the modulation of fish inflammatory processes caused by internal or external factors.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Lourenco Ramos-Pinto, Marina Machado, Josep Calduch-Giner, Jaume Perez-Sanchez, Jorge Dias, Luis E. C. Conceicao, Tome S. Silva, Benjamin Costas
Summary: The study aimed to evaluate the effects of histidine, taurine, and threonine supplementation on the immune status of gilthead seabream. The results showed that dietary supplementation with these amino acids had mild immune-modulation effects on gilthead seabream, particularly noteworthy for further study under disease challenge conditions.
Article
Fisheries
Catarina Basto-Silva, Paula Enes, Aires Oliva-Teles, Encarnacion Capilla, Ines Guerreiro
Summary: This study evaluated the effects of dietary protein/carbohydrate ratio and feeding frequency on the growth, metabolism, and economic efficiency of gilthead seabream juveniles. The results showed that feeding a diet with a protein/carbohydrate ratio of 40%/20% twice a day was the most suitable strategy, as it improved fish growth and economic efficiency.
Article
Biochemistry & Molecular Biology
Paulo Santos, Diogo Peixoto, Ines Ferreira, Ricardo Passos, Pedro Pires, Marco Simoes, Pedro Pousao-Ferreira, Teresa Baptista, Benjamin Costas
Summary: Photobacteriosis is a bacterial disease that affects various marine species globally, leading to significant economic losses. Despite studies on pathogen virulence and resistance, information on host defense mechanisms post-infection remains limited. This study focused on innate immune responses of farmed juvenile gilthead seabream after infection with Photobacterium damselae subsp. piscicida, revealing marked changes in blood parameters and gene expression related to inflammation and phagocytic activity. Further research on different fish species and pathogens could help identify health biomarkers for disease prevention in aquaculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Jhon A. Serna-Duque, Alberto Cuesta, M. Angeles Esteban
Summary: Host defense peptides, particularly hepcidins, play a crucial role in the innate immune system of vertebrates. The study on hepcidins in gilthead seabream revealed a massive duplication of hamp genes on chromosome 17, suggesting high adaptation and diversity within this species. Additionally, the research indicated different immune roles for hepcidins in seabream and showcased specific-tissue expression profiles in response to bacterial challenge, potentially leading to a deeper understanding of innate immunity in fish and vertebrates.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Oguz Tasbozan, Celal Erbas, Mehtap Bayir, Erdal Ozdemir, Goekhan Arslan, Abdulkadir Bayir
Summary: The molecular characteristics and tissue disruption of fabp genes in gilthead seabream were investigated, and their expression levels were found in the fish fed diets with different vegetable oil sources. The study provides further evidence that dietary fatty acids affect fabp mRNA expressions in S. aurata. This may be useful in the nutritional control of fabp genes in marine fish fed VO-based diets at low water temperatures.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Immunology
Pablo Castejon, Isabel Cabas, Victoria Gomez, Elena Chaves-Pozo, Isabel Cerezo-Ortega, Miguel Angel Morinigo, Eduardo Martinez-Manzanares, Jorge Galindo-Villegas, Alfonsa Garcia-Ayala
Summary: Our study revealed the intrinsic relationship among estrogens, their associated receptors, and immunization in the ubiquitous fish immune regulation and the subtle but significant crosstalk with the gut endobolome.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Rocio Leiva-Rebollo, Juan Gemez-Mata, Dolores Castro, Juan J. Borrego, Alejandro M. Labella
Summary: The DNA vaccine based on the major capsid protein of Lymphocystis disease virus 3 has been found to induce changes in the expression of immune-related genes in vaccinated gilthead seabream. The vaccine can effectively decrease viral replication and modulate the inflammatory and interferon response, leading to control of the infection progression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Wei Wang, Taja Lozar, Athena E. Golfinos, Denis Lee, Ellery Gronski, Ella Ward-Shaw, Mitchell Hayes, Justine Y. Bruce, Randall J. Kimple, Rong Hu, Paul M. Harari, Jin Xu, Aysenur Keske, Paul M. Sondel, Megan B. Fitzpatrick, Huy Q. Dinh, Paul F. Lambert
Summary: This study investigates the association between high expression of stress keratin 17 (K17) and poor survival and resistance to immunotherapy in human head and neck squamous cell carcinoma (HNSCC). Through the use of a mouse model and patients with HNSCC, it is found that K17 expression plays an important role in regulating the tumor microenvironment and immune responsiveness. Knocking out K17 results in slower tumor growth, increased infiltration of immune cells, and elimination of tumors upon immune-checkpoint blockade treatment. High K17 expression is associated with poor response to pembrolizumab treatment in HNSCC patients.
CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jose Carlos Campos-Sanchez, Javier Mayor-Lafuente, Francisco A. Guardiola, Maria angeles Esteban
Summary: In this study, the conservation of inflammation-related proteins in gilthead seabream was explored through computational analysis, with the highest identity found in proteins of the large yellow croaker. Gene expression analysis revealed that the mechanisms required to terminate the inflammatory response and restore skin homeostasis were activated between 12 and 24 hours after carrageenin injection in gilthead seabream specimens. This research contributes to identifying the main mechanisms of acute inflammatory response and validates the use of carrageenin as an inflammation model in fish.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Fisheries
Jose Carlos Campos-Sanchez, Enrica Vitarelli, Francisco A. Guardiola, Diana Ceballos-Francisco, Jose Maria Garcia Beltran, Antonio Ieni, Maria Angeles Esteban
Summary: This study evaluated local inflammation mechanisms in the skin of gilthead seabream induced by carrageenin, finding an increase in skin mucus-secreting cells and acidophilic granulocytes after injection. Gene expression of various markers and proinflammatory genes was also up-regulated post-injection. Results indicate the chronological involvement of important immune cells in resolving inflammation in the fish skin.
JOURNAL OF FISH DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
G. Rodriguez-Valdez, R. Romero-Geraldo, G. Medina-Basulto, M. Reyes-Becerril, C. Angulo
Summary: Excessive use of antibiotics has led to an increase in antibiotic-resistant pathogenic bacteria. Hypersaline and hyperthermal environments promote the development of microorganisms with immunostimulatory activity. This study identified marine bacteria with immunostimulatory activity and positive effects on mouse leukocytes, potentially offering a new approach to fighting infectious diseases.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Review
Fisheries
Jesus Antonio Lopez-Carvallo, Jose Manuel Mazon-Suastegui, Guadalupe Fabiola Arcos-Ortega, Miguel Angel Hernandez-Onate, Dariel Tovar-Ramirez, Fernando Abasolo-Pacheco, Milagro Garcia-Bernal
Summary: Highly diluted bioactive compounds and immunomodulatory compounds show potential in improving marine organism production efficiency and immune system response, thus reducing the use of chemotherapy and antibiotics.
REVIEWS IN AQUACULTURE
(2022)
Article
Fisheries
Jose Manuel Mazon-Suastegui, Guadalupe Fabiola Arcos-Ortega, Cesar Lodeiros, Dariel Tovar-Ramirez, Jesus Antonio Lopez-Carvallo
Summary: This study evaluates the growth and survival of the Catarina scallop in different stocking densities. The results show that lower stocking densities can promote growth and survival, and reduce the time required to reach the pediveliger stage.
AQUACULTURE INTERNATIONAL
(2022)
Article
Chemistry, Inorganic & Nuclear
Gurjaspreet Singh, Diksha, Mohit, Suman, Sushma, Anita Devi, Sofia Gupta, Cristobal Espinosa-Ruiz, Maria Angeles Esteban
Summary: In this study, Schiff base derived organosilatranes with pyridine moiety were successfully synthesized using a simple method. The structure of the compounds was determined through various spectroscopic studies. The chemosensor exhibited high selectivity and sensitivity towards Zn2+ ions, as evidenced by significant changes in UV-vis and fluorometric spectra. Immobilizing the organosilatrane on silica nanoparticles surface enhanced its binding capacity for Zn2+ ions and showed potential for water treatment applications. Additionally, the synthesized organosilatranes showed promising pharmacokinetic profiles and cytotoxicity in biological field applications.
INORGANICA CHIMICA ACTA
(2022)
Article
Chemistry, Inorganic & Nuclear
Gurjaspreet Singh, Suman, Diksha, Mohit, Sofia Gupta, Yamini Thakur, D. Gonzalez-Silvera, Cristobal Espinosa-Ruiz, Maria Angeles Esteban
Summary: In this study, a chemosensor for the detection of Al3+ ions was successfully synthesized, which showed excellent detection sensitivity and interference resistance. The synthesized compounds also exhibited good antioxidant properties.
INORGANICA CHIMICA ACTA
(2022)
Article
Fisheries
Andressa Teles, Carlos Alfonso Alvarez-Gonzalez, Raul Llera-Herrera, Enric Gisbert, Joan Salas-Leiva, Maria Del Carmen Rodriguez-Jaramillo, Dariel Tovar-Ramirez
Summary: This study aimed to investigate the effect of Debaryomyces hansenii yeast on the growth, development, and skeletal deformities of longfin yellowtail larvae. The results showed that larvae fed with D. hansenii yeast had higher survival and growth rates, increased bone mineralization, reduced skeletal deformities, enhanced intestinal function and digestive enzyme activity.
Article
Agriculture, Dairy & Animal Science
Marcos Acosta, Eduardo Quiroz, Dariel Tovar-Ramirez, Vania Palma Roberto, Jorge Dias, Paulo J. Gavaia, Ignacio Fernandez
Summary: This study evaluated the best storing conditions for aquafeeds supplemented with VK1 and the effect of VK1 supplementation on the gut microbiota of Senegalese sole juveniles. The results showed that high levels of VK1 required storage at low temperatures to ensure stability, and VK1 supplementation improved the balance of gut bacterial communities, leading to better fish health.
Article
Biochemistry & Molecular Biology
Aurora Araiza-Campos, Guillermo Herrera-Perez, Joan S. Salas-Leiva, Margarita Chavez-Beltran de Rio, Alejandro Aguirre-Hernandez, Antonia Luna-Velasco, Dayana Salas-Leiva, Eduardo Campos-Chavez, Dariel Tovar-Ramirez, Alejandro Romo-Chacon, Erasmo Orrantia-Borunda, Francisco Paraguay-Delgado
Summary: The current study compares the antibacterial activity of zinc oxide nanostructures. The quasi-diamond platelet-shaped nanostructures showed enhanced antibacterial activity compared to the quasi-round agglomerated particles. The morphology plays a crucial role in the antimicrobial activity, while the enrichment of oxygen vacancies does not have a significant effect.
Article
Microbiology
Luis Daniel Moreno-Figueroa, Eduardo Quiroz-Guzman, Dariel Tovar-Ramirez, Gabriela Palestino, Candida A. Cisneros-Covarrubias, Luis Hernandez-Adame
Summary: Phage therapy is a promising alternative to control bacterial diseases. This research evaluated the viability of lyophilized vibrio phage vB_Pd_PDCC-1 using trehalose as a preservative excipient. The results showed that trehalose is beneficial in maintaining the viability of post-lyophilization phages, even at room temperature. The use of lyophilized phage PDCC-1 increased brine shrimp survival and reduced Vibrio concentrations.
CURRENT MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ignasi Sanahuja, Alberto Ruiz, Joana P. P. Firmino, Felipe E. E. Reyes-Lopez, Juan B. B. Ortiz-Delgado, Eva Vallejos-Vidal, Lluis Tort, Dariel Tovar-Ramirez, Isabel M. M. Cerezo, Miguel A. A. Morinigo, Carmen Sarasquete, Enric Gisbert
Summary: The supplementation of Debaryomyces hansenii in the diet of gilthead seabream improved growth and feed efficiency, while enhancing intestinal condition and modulating gut microbiota. Transcriptomic analysis revealed that this probiotic yeast promoted metabolic pathways, antioxidant mechanisms, and immune processes, contributing to the defensive capacity and homeostasis of the intestine.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Immunology
Ignasi Sanahuja, Laura Fernandez-Alacid, Silvia Torrecillas, Alberto Ruiz, Eva Vallejos-Vidal, Joana P. Firmino, Felipe E. Reyes-Lopez, Lluis Tort, Dariel Tovar-Ramirez, Antoni Ibarz, Enric Gisbert
Summary: This study examines the effects of supplementing Debaryomyces hansenii as a probiotic in a low fish meal-based diet on the skin mucosal tissue in Sparus aurata. The results show that the probiotic supplementation improves fish growth and feed conversion ratio, and modulates immune and metabolic processes in the skin.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Norma Flores-Holguin, Joan S. Salas-Leiva, Erick J. Nunez-Vazquez, Dariel Tovar-Ramirez, Daniel Glossman-Mitnik
Summary: Marine toxins pose significant risks to marine ecosystems and human health. Understanding their structures and properties is crucial for mitigating risks and exploring their potential as therapeutic agents. This study compares four marine toxins, examining their reactivity properties and bioavailability. By investigating their similarities, valuable insights for new drug development can be gained. Exploring the lipophilic and hydrophilic properties in drug design is important for drug distribution and interaction. By elucidating shared molecular properties, this research aims to guide future drug discovery efforts and contribute to the field of molecular toxinology. The findings expand knowledge on toxins, deepen understanding of their bioactivities, and unlock new therapeutic possibilities.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Colleen Guinle, Erick Julian Nunez-Vazquez, Leyberth Jose Fernandez-Herrera, Daniela Alejandra Corona-Rojas, Dariel Tovar-Ramirez
Summary: Harmful algal blooms can have noxious effects on marine organisms. This study found that saxitoxin had toxicological effects on the embryonic and early larval stages of Seriola rivoliana. The toxin reduced hatching percentage, increased enzyme activity, and induced overexpression of genes. The effects were more pronounced in young larvae than in embryos.
Article
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
Summary: This study provides the first report on the digestive tract pH of the pygmy octopus. The pH of the digestive tract regions was found to be lower than 7.0 and did not differ significantly between wild and captive octopuses. The digestive gland had the lowest pH values, but they were still above 5.0.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)