Article
Chemistry, Applied
Qian Hong, Yu-Lei Chen, Duanquan Lin, Ru-Qing Yang, Kai-Yuan Cao, Ling-Jing Zhang, Yi-Ming Liu, Le-Chang Sun, Min-Jie Cao
Summary: In this study, the PPO gene from Litopenaeus vannamei (Lv-PPO) was cloned, expressed, and purified. The recombinant Lv-PPO (rLv-PPO) was shown to be biologically active and influenced by metal ions Cu2+ and Zn2+. A specific antibody against rLv-PPO was prepared, and the levels of PPO were found to be highest in the hemolymph of shrimp.
Article
Fisheries
Chenxi Zhu, Guoxing Liu, Anisah Lee Binti Abdullah, Mingming Han, Qichen Jiang, Yiming Li
Summary: This study investigated the effects of nanoplastics on Litopenaeus vannamei. The results showed that nanoplastics exposure activated the lysosome pathway and affected the PI3K-Akt signaling pathway. In addition, nanoplastics disrupted hepatic lipid metabolism, caused oxidative stress, and damaged tissue structure in L. vannamei.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jiamin Feng, Yongxiong Huang, Meiling Huang, Junliang Luo, Liwen Que, Shiping Yang, Jichang Jian
Summary: In this study, a novel C-type lectin (CTL) called perlucin-like protein (PLP) was identified in Litopenaeus vannamei. PLP could be activated in response to Vibrio harveyi infection and could bind and agglutinate various bacteria. PLP was involved in stabilizing the expression of immune-related and apoptosis genes and reducing bacterial load in the hepatopancreas.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Hongtao Liu, Shengtao Guo, Rong Wang, Yugui He, Qiong Shi, Zhaobin Song, Mingqiu Yang
Summary: The study used proteomic sequencing of hepatopancreas in Vibrio harveyi-infected whiteleg shrimp and found a significant number of DEPs, specifically showing changes in the expression levels of C-type lectins after infection. Different members of the CTL family exhibit varied responses to Vibrio harveyi infection, suggesting potential differential functions and providing theoretical guidance for further research on immune responses and pathogen infections in shrimps.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Acoustics
Tong Zhang, Jie Wang, Jiaqi Feng, Yaqiong Liu, Ran Suo, Jingyu Jin, Wenxiu Wang
Summary: This study investigated the effects of different ultrasonic pretreatments on the gel properties of shrimp surimi. The results showed that proper ultrasound treatment could improve the gel properties by expanding the protein and exposing more functional groups. The study provides a new technical means to improve the gel properties of shrimp surimi.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Xiaoxi Chang, Tuo Zhang, Jiachen Zang, Chenyan Lv, Guanghua Zhao
Summary: This study successfully isolated and characterized natural LvEnolase from L. vannamei, and conducted comprehensive analyses on its amino acid sequence, protein structure, and enzyme activity. These findings contribute to a better understanding of the enolase family and provide valuable support for further investigations into the functions and characteristics of LvEnolase.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Immunology
Xianliang Zhao, Jie Qiao, Pei Zhang, Zehui Zhang, Jude Juventus Aweya, Xiaohan Chen, Yongzhen Zhao, Yueling Zhang
Summary: In this study, the proteomics approach was used to characterize the hemocyanin expression profiles from Litopenaeus vannamei, revealing molecular diversity at the protein level. The research demonstrated that the glycan modification may contribute to the diversity and multiple immune activities of hemocyanin, which plays a crucial role in recognizing different pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Yafei Duan, Shimin Zeng, Zijun Lu, Xueming Dan, Zequan Mo, Yifu Xing, Jiasong Zhang, Yanwei Li
Summary: In this study, the effects of Microcystin-LR (MC-LR) on lipid metabolism and lipidomic responses in Litopenaeus vannamei hepatopancreas were investigated. The results showed that MC-LR exposure caused tissue damage and decreased the activities of several lipase isoenzymes in the hepatopancreas. Additionally, the relative gene expression levels of key genes involved in lipolysis, lipogenesis, and fatty acid beta-oxidation were increased. Lipidomic analysis revealed that MC-LR exposure resulted in increased levels of glycerophospholipids, sphingolipids, and cholesteryl ester, while the levels of phosphatidylinositol and triglyceride were decreased. These findings provide important insights into the effects of MC-LR on lipid metabolism and lipidomic homeostasis in shrimp hepatopancreas.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Fisheries
Junliang Luo, Yanghui Chen, Yongxiong Huang, Jiamin Feng, Yunhao Yuan, Jichang Jian, Shuanghu Cai, Shiping Yang
Summary: In this study, a novel CTL of Litopenaeus vannamei, LvCTL7, was successfully cloned and shown to have microbial agglutination and immunoregulatory activity. LvCTL7 was mainly expressed in hepatopancreas, muscle, gill, and eyestalk, and it could bind to Gram-positive and Gram-negative bacteria. LvCTL7 was involved in the innate immune response against Vibrio infection in L. vannamei.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Mirasha Hasanthi, Kyeong-Jun Lee
Summary: This study evaluated the dietary requirement of niacin and its effects on the growth, feed utilization, immunity, and disease resistance of Pacific white shrimp. The results showed that increasing niacin levels in the diet improved the growth performance, feed utilization efficiency, and disease resistance of the shrimp. The optimum niacin requirement for Pacific white shrimp was estimated to be around 130 mg/kg diet.
Article
Biochemistry & Molecular Biology
Ying Pang, Xiaojun Zhang, Jianbo Yuan, Xiaoxi Zhang, Jianhai Xiang, Fuhua Li
Summary: The insulin signaling pathway is crucial in various physiological aspects, with insulin-like growth factor binding proteins (IGFBPs) playing a key role. This study identified and analyzed three IGFBP genes in the Pacific white shrimp genome, showing that LvIGFBP1 has multiple domains and higher expression levels compared to LvIGFBP2 and LvIGFBP3. RNA interference of LvIGFBP1 resulted in a significant decrease in shrimp body weight increment, indicating its importance in growth regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Shuqing Si, Xiaojun Zhang, Yang Yu, Xiaoxi Zhang, Xiaoyun Zhong, Jianbo Yuan, Song Yang, Fuhua Li
Summary: Considering the important role of Monocyte to macrophage differentiation factor 2 gene (Mmd2) in growth and development, we analyzed the gene structure, expression profiles, and RNA interference of LvMmd2 in L. vannamei. LvMmd2 gene sequence was found to be highly conserved in transmembrane regions and widely expressed in different tissues, with the highest expression level in the eye stalk. Knockdown of LvMmd2 significantly promoted body length and body weight gain, indicating its role as a growth suppressor. Transcriptome analysis revealed significant changes in growth, metabolism, and immune-related signal pathways. These results enhance our understanding of the conservative structure and function of LvMmd2 gene and have potential applications in genetics and breeding.
FRONTIERS IN GENETICS
(2023)
Article
Fisheries
Kokila Sanjeewani, Kyeong-Jun Lee
Summary: This study aimed to determine the dietary riboflavin requirement and its effects on the growth performance, feed utilization, innate immunity, and diet digestibility of Litopenaeus vannamei. The results showed that riboflavin significantly improved the growth performance, non-specific immunity, and intestinal morphology of the shrimp, with an optimal riboflavin requirement of approximately 40.9 mg/kg diet.
AQUACULTURE NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Hao Yang, Xiaoli Chen, Zhi Li, Xugan Wu, Mingyu Zhou, Xin Zhang, Yujie Liu, Yuying Sun, Chunhua Zhu, Qiuhui Guo, Ting Chen, Jiquan Zhang
Summary: This study provides basic knowledge about the synthesis and inactivation pathway of prostaglandins in crustaceans, which is more complete compared to insects and mollusks. The expression patterns of different genes in various tissues and developmental processes were analyzed, revealing their specific roles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Roushon Jamal
Summary: The commercial cultivation of Litopenaeus vannamei has greatly benefited the global shrimp industry in the past decade. However, there are no academic sources supporting its commercial farming in Bangladesh, which is an important player in the global shrimp industry. The declining shrimp industry in Bangladesh, caused by the low production of traditionally cultivated black tiger shrimp and its susceptibility to diseases, calls for immediate policy measures to introduce high-yielding Litopenaeus vannamei species.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Hui Yang, Shihao Li, Fuhua Li, Xinjia Lv, Jianhai Xiang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2015)
Article
Fisheries
Hui Yang, Shihao Li, Fuhua Li, Rong Wen, Jianhai Xiang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Jiquan Zhang, Tianshu Gui, Jing Wang, Jianhai Xiang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2015)
Article
Biochemistry & Molecular Biology
Jing Wang, Jiquan Zhang, Fengge Song, Tianshu Gui, Jianhai Xiang
Article
Multidisciplinary Sciences
Yingli Shi, Marcus Soderlund, Jianhai Xiang, Yuanan Lu
Article
Multidisciplinary Sciences
Yang Yu, Xiaojun Zhang, Jianbo Yuan, Fuhua Li, Xiaohan Chen, Yongzhen Zhao, Long Huang, Hongkun Zheng, Jianhai Xiang
SCIENTIFIC REPORTS
(2015)
Article
Limnology
Zhang Chengsong, Li Fuhua, Xiang Jianhai
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2015)
Article
Fisheries
Zhiwei Wang, Shihao Li, Yang Yu, Kuijie Yu, Xiaojun Zhang, Jianhai Xiang, Fuhua Li
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Environmental Sciences
Fei Liu, Shihao Li, Yang Yu, Mingzhe Sun, Jianhai Xiang, Fuhua Li
Article
Biochemistry & Molecular Biology
Ying Pang, Xiaojun Zhang, Jianbo Yuan, Xiaoxi Zhang, Jianhai Xiang, Fuhua Li
Summary: The insulin signaling pathway is crucial in various physiological aspects, with insulin-like growth factor binding proteins (IGFBPs) playing a key role. This study identified and analyzed three IGFBP genes in the Pacific white shrimp genome, showing that LvIGFBP1 has multiple domains and higher expression levels compared to LvIGFBP2 and LvIGFBP3. RNA interference of LvIGFBP1 resulted in a significant decrease in shrimp body weight increment, indicating its importance in growth regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Medicinal
Xiaojun Zhang, Jianbo Yuan, Fuhua Li, Jianhai Xiang
Summary: Chitin synthesis genes typically occur in single or two copies in crustaceans, while chitin degradation genes are all multiple copies. Chitinase genes are significantly expanded in most crustacean genomes. The gene structure and expression pattern of these genes are similar to those of insects, albeit with some specific characteristics.
Article
Chemistry, Medicinal
Mingzhe Sun, Shihao Li, Xinjia Lv, Jianhai Xiang, Yuanan Lu, Fuhua Li
Summary: By studying the antimicrobial peptides in shrimp, differences in disease resistance among different shrimp species were discovered, providing evidence for understanding the distinct disease resistance among shrimp species and laying out new research ground for the testing and development of antimicrobial peptides for shrimp disease control.
Article
Engineering, Marine
Kangxuan Chen, Shihao Li, Jianhai Xiang, Amir Sagi, Fuhua Li
Summary: A comparative transcriptome analysis of the androgenic gland in L. vannamei revealed that 20-hydroxyecdysone could dose-dependently inhibit the expression of Lv-IAG at different molting stages, providing new insights into the regulatory mechanism of ecdysteroids on Lv-IAG expression and the endocrine axis between eyestalk and AG in male crustaceans.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Chengzhang Liu, Jianbo Yuan, Xiaojun Zhang, Songjun Jin, Fuhua Li, Jianhai Xiang
Summary: Research suggests that genomic tRNA content influences protein synthesis efficiency in sea cucumbers, while codon usage optimization is mostly constrained by a gene's amino acid sequence. The positive correlation between genomic tRNA content and amino acid content in natural food particles of sea cucumbers enhances their efficiency in protein synthesis.
Article
Biology
Jianbo Yuan, Xiaojun Zhang, Qi Kou, Yamin Sun, Chengzhang Liu, Shihao Li, Yang Yu, Chengsong Zhang, Songjun Jin, Jianhai Xiang, Xinzheng Li, Fuhua Li
Summary: The study reveals that the deep-sea giant isopod Bathynomus jamesi has a large genome size primarily due to transposable elements, with expanded gene families related to thyroid and insulin hormone signaling contributing to its large body size. Transcriptomic analysis suggests specific gene families related to glycolysis and vesicular transport are expressed in its digestive organs. Comparative genomics and gene expression analyses show that B. jamesi has inefficient lipid degradation, low basal metabolic rate, and bulk food storage, indicating that giant isopods have evolved efficient mechanisms for nutrient absorption, storage, and utilization to sustain their large body size in deep-sea environments.