Article
Genetics & Heredity
Yodo Sugishita, Enes Taylan, Tai Kawahara, Bunyad Shahmurzada, Nao Suzuki, Kutluk Oktay
Summary: The study revealed that slow freezing and vitrification with open or closed devices showed no significant differences in the survival of primordial follicles, DNA damage, and apoptosis rates in ovarian tissues after cryopreservation and thawing/warming.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Katerina Chatzimeletiou, Antonia Sioga, Nikos Petrogiannis, Yannis Panagiotidis, Marialena Prapa, Antonios Patrikiou, Basil C. Tarlatzis, Grigoris Grimbizis
Summary: The study found that vitrified embryos had higher cell damage compared to fresh embryos, regardless of whether they were vitrified in open or closed systems. However, both Day 5 and Day 3 biopsied embryos showed no adverse effects on viability and ultrastructure following vitrification in open and closed systems.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Endocrinology & Metabolism
Hyun Sun Kong, Yeon Hee Hong, Jaewang Lee, Hye Won Youm, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Summary: The use of antifreeze protein treatment during the warming process has been shown to improve the quality of bovine ovarian tissue after xenotransplantation, with increased morphological follicle normality and decreased apoptotic rates.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Dalin Wang, Menghui Geng, Dongying Gan, Gege Han, Gao Gao, Aying Xing, Yugui Cui, Yanqiu Hu
Summary: Resveratrol could improve the quality of cryopreserved mouse ovarian tissue after transplantation and the embryo outcome, through anti-inflammatory and antioxidative mechanisms, as shown by increased number of retrieved oocytes and improved fertilization and cleavage in the resveratrol group compared to the saline group.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
H. Ali Hassan, P. Banchi, R. Chayaa, O. B. Pascottini, L. Maniscalco, S. Iussich, K. Smits, A. Van Soom
Summary: In order to achieve optimal vitrification, the challenges lie in tissue structure and fragment size which affect cooling velocity. Thin ovarian tissue fragments with a larger surface area and higher solute penetration are desired. Cryoprotectant concentration is another critical factor, as high concentrations can lead to toxicity and local apoptosis. Two experiments were conducted to address these challenges. The results demonstrated that smaller sized fragments vitrified in a solution supplemented with sucrose had higher percent of intact follicles after vitrification. The optimized carrier medium HTS did not show additional protective effect on follicular morphology or apoptotic rate.
Article
Agriculture, Dairy & Animal Science
Daniela Bebbere, Amir Arav, Stefano Mario Nieddu, Giovanni Pietro Burrai, Sara Succu, Pasquale Patrizio, Sergio Ledda
Summary: Freeze-drying is a method for preserving cells and tissues, offering cost savings and improved stability; sheep ovarian tissue survived the process, maintaining its structure and responding to specific genes.
Article
Reproductive Biology
Fei Wang, Yuan Tian, Liwen Huang, Tian Qin, Wenye Ma, Chengbin Pei, Bo Xu, Hang Han, Xinrui Liu, Pengge Pan, Xiaoli Yu, Qin Chang, Yanrong Wang, Shuya Zhang, Xiuying Pei
Summary: This study aims to investigate the protective effects and underlying mechanisms of follicle-stimulating hormone (FSH) and Sphingosine-1-phosphate (S1P) on vitrified and post-transplantation ovaries. The results showed that co-administration of FSH and S1P can preserve the primordial follicle pool, inhibit follicular atresia, promote angiogenesis, and restore ovarian endocrine function.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Obstetrics & Gynecology
Jennifer Hajek, Rebecca Baron, Nathallie Sandi-Monroy, Stefanie Schansker, Beate Schoepper, Marion Depenbusch, Askan Schultze-Mosgau, Kay Neumann, Friedrich Gagsteiger, Soeren von Otte, Georg Griesinger
Summary: The study showed that a semi-automated closed vitrification method has similar clinical outcomes for survival rate of 2PN oocytes compared to a manual open system, but has higher procedure duration and less staff convenience.
HUMAN REPRODUCTION
(2021)
Review
Obstetrics & Gynecology
L. Cacciottola, J. Donnez, M. M. Dolmans
Summary: Gonadotoxic treatments like chemotherapy or radiotherapy and ovarian surgery can lead to premature ovarian insufficiency. Cryopreservation of oocytes and ovarian tissue shows promising results in terms of pregnancy and live birth rates. Ovarian tissue transplantation is a potential future option for complete restoration of ovarian function.
BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY
(2022)
Article
Obstetrics & Gynecology
Yodo Sugishita, Lingbo Meng, Yuki Suzuki-Takahashi, Sandy Nishimura, Sayako Furuyama, Atsushi Uekawa, Akiko Tozawa-Ono, Junko Migitaka-Igarashi, Tomoe Koizumi, Hibiki Seino, Yasunori Natsuki, Manabu Kubota, Junki Koike, Keisuke Edashige, Nao Suzuki
Summary: After thawing/warming, at least 60 minutes of diffusion washing in media was necessary to significantly reduce the residual cryoprotectants in ovarian tissues frozen by slow freezing or vitrification.
HUMAN REPRODUCTION
(2022)
Article
Plant Sciences
Cassandra D. Downey, Gregory Golenia, Ekaterina A. Boudko, Andrew Maxwell P. Jones
Summary: This study developed a functional cryopreservation system for long-term germplasm preservation in Cannabis sativa, successfully tested across 13 genotypes. Plants grown from cryopreserved samples were morphologically and chemically similar to control plants for most major traits, but some differences were observed in the minor cannabinoid and terpene profiles.
Review
Obstetrics & Gynecology
Marisa Kometas, Gregory M. Christman, Joseph Kramer, Alice Rhoton-Vlasak
Summary: The effectiveness of vitrification compared to slow freezing for preserving ovarian tissue remains controversial, with limited clinical data and inconsistent findings from biochemical studies. While early clinical outcomes suggest favorability towards vitrification, further evaluation is needed to determine its long-term efficacy. Despite potential lack of difference in outcomes between the two methods, the cost-effectiveness and efficiency of vitrification make it a more accessible option.
REPRODUCTIVE SCIENCES
(2021)
Article
Reproductive Biology
Lingbo Meng, Yodo Sugishita, Sandy Nishimura, Atsushi Uekawa, Yuki Suzuki-Takahashi, Nao Suzuki
Summary: This study aimed to investigate the optimal culture time and viability of postwarmed ovarian tissues before transplantation. The findings suggest that a culture time of 1-2 hours is optimal for vitrified-warmed tissues.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Xia Zhang, Xin Liu, Xiao-Li Liu, Dan-Ya Wu, Kai Zhou, Zhi-Sheng Yu, Cheng-Li Dou, Tian Xu, Mei Yu, Yi-Liang Miao
Summary: In this study, a quick and simple method for cryopreserving porcine ear tissue was explored. The tissues were cut into strips and frozen using direct cover vitrification (DCV) with a cryoprotectant solution containing 15% EG, 15% DMSO, and 0.1 M trehalose. Histological and ultrastructural evaluation confirmed the normal tissue structure of thawed tissues. Viable fibroblasts could be derived from these tissues even after 6 months of cryopreservation, with no cell apoptosis and normal karyotypes, making them suitable for nuclear transfer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Marie-Madeleine Dolmans, Camille Hossay, Thu Yen Thi Nguyen, Catherine Poirot
Summary: Chemotherapy, pelvic radiotherapy, and ovarian surgery can have harmful effects on the reproductive system, leading to early depletion of the ovarian reserve and increased risk of future fertility problems. Factors such as ovarian reserve, type of treatment, dose, and patient age determine the extent of this risk.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Selcuk Erkilinc, Enes Taylan, Varol Gulseren, Gamze Erkilinc, Tugba Karadeniz, Mustafa Bagci, Orhan Temel, Ulas Solmaz, Mehmet Gokcu, Muzaffer Sanci
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2018)
Editorial Material
Obstetrics & Gynecology
Kutluk Oktay, Enes Taylan, Tai Kawahara, Giulia M. Cillo
FERTILITY AND STERILITY
(2019)
Article
Obstetrics & Gynecology
Selcuk Erkilinc, Enes Taylan, Volkan Karatasli, Ipek Uzaldi, Tugba Karadeniz, Mehmet Gokcu, Muzaffer Sanci
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2019)
Review
Oncology
Enes Taylan, Kutluk Oktay
GYNECOLOGIC ONCOLOGY
(2019)
Article
Obstetrics & Gynecology
Ahmet Mete Ergenoglu, Ismet Hortu, Enes Taylan, Ahmet Ozgur Yeniel, Ali Akdemir, Cagdas Sahin, Nedim Karadadas
JOURNAL OF GYNECOLOGY OBSTETRICS AND HUMAN REPRODUCTION
(2020)
Article
Obstetrics & Gynecology
Kutluk H. Oktay, Giuliano Bedoschi, Shari B. Goldfarb, Enes Taylan, Shiny Titus, Glenn E. Palomaki, Tessa Cigler, Mark Robson, Maura N. Dickler
FERTILITY AND STERILITY
(2020)
Article
Oncology
Enes Taylan, Fouzia Zayou, Ramachandran Murali, Beth Y. Karlan, Stephen J. Pandol, Mouad Edderkaoui, Sandra Orsulic
GYNECOLOGIC ONCOLOGY
(2020)
Article
Cell Biology
Ye Hu, Barbie Taylor-Harding, Yael Raz, Marcela Haro, Maria Sol Recouvreux, Enes Taylan, Jenny Lester, Joshua Millstein, Ann E. Walts, Beth Y. Karlan, Sandra Orsulic
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Shari B. Goldfarb, Volkan Turan, Giuliano Bedoschi, Enes Taylan, Nadia Abdo, Tessa Cigler, Heejung Bang, Sujita Patil, Maura N. Dickler, Kutluk H. Oktay
Summary: This study found that both AC-based and CMF chemotherapy regimens significantly compromise ovarian reserve in women with breast cancer, with no significant recovery beyond 12 months post-chemotherapy. In contrast, tamoxifen-only treatment does not seem to alter ovarian reserve.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Meeting Abstract
Obstetrics & Gynecology
Kutluk H. Oktay, Volkan Turan, Giuliano Bedoschi, Enes Taylan, Heejung Bang, Maura Dickler, Shari Goldfarb
FERTILITY AND STERILITY
(2020)
Meeting Abstract
Obstetrics & Gynecology
K. H. Oktay, G. Bedoschi, T. Kawahara, L. Marin, Y. Sugisihita, E. Taylan, L. Ursillo
HUMAN REPRODUCTION
(2019)
Meeting Abstract
Oncology
K. Oktay, G. Bedoschi, S. B. Goldfarb, E. Taylan, S. Titus, G. E. Palomaki, T. Cigler, M. Robson, M. N. Dickler
Meeting Abstract
Oncology
S. Erkilinc, E. Taylan, K. Volkan, I. Uzaldi, B. Can, C. Ilker, O. Temel, T. Karadeniz, M. Gokcu, M. Sanci
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2018)
Article
Medicine, General & Internal
Selcuk Erkilinc, Enes Taylan, Orhan Temel, Tugba Karadeniz, Mehmet Gokcu, Muzaffer Sanci
CUKUROVA MEDICAL JOURNAL
(2018)
Meeting Abstract
Oncology
Kutluk Oktay, Enes Taylan, Yodo Sugishita, Tai Kawahara, Nao Suzuki
