Variants of the WNT7A gene in Chinese patients with mullerian duct abnormalities

Objective: To search for WNT7A gene mutations in a cohort of 191 Chinese Han patients with mullerian duct abnormalities (MDAs). Design: Phenotypic and mutational study. Setting: University hospital. Patient(s): A total of 191 Chinese Han patients with MDAs and 192 healthy control individuals. Intervention(s): Genomic DNA extracted from blood samples, all coding regions amplified by polymerase chain reaction (PCR) then directly sequenced to screen variants. Main Outcome Measure(s): Not applicable. Result(s): The sequence analysis revealed one novel synonymous variant and three known single-nucleotide polymorphisms (SNPs). Conclusion(s): The results indicate that mutations in the coding sequence of WNT7A are not responsible for mullerian duct abnormalities in the Chinese population. (Fertil Steril(R) 2012;97:391-4. (C) 2012 by American Society for Reproductive Medicine.)