Article
Agriculture, Dairy & Animal Science
Jana Rychtarova, Alena Langerova, Helena Fulka, Pasqualino Loi, Michal Benc, Josef Fulka
Summary: The long-term storage of biological material is crucial for various fields, and a simple approach for evaluating sperm DNA damage level has been proposed to address the issue of limited frozen biological material quantities.
Article
Reproductive Biology
Luisa Ramirez-Agamez, Camilo Hernandez-Aviles, Dickson D. Varner, Charles C. Love
Summary: This study explores stallion-associated factors in intracytoplasmic sperm injection (ICSI) using frozen/thawed sperm. The results indicate that the composition of freezing extender affects the developmental competence of sperm and in vitro-matured equine oocytes after ICSI. Furthermore, there were no significant differences observed when using ejaculated or epididymal sperm, or sperm with different morphological features in ICSI outcomes.
Article
Agriculture, Dairy & Animal Science
Asmarani Kusumawati, Faisal Amri Satrio, Rhesti Indriastuti, Zulfi Nur Amrina Rosyada, Berlin Pandapotan Pardede, Muhammad Agil, Bambang Purwantara
Summary: This study analyzed the association between various alterations in sperm morphology and DNA damage level, PRM deficiency, and the abundance of PRM1 gene and protein in sperm. The results showed that each parameter is correlated with one another, indicating the essential role of gene expression and PRM1 protein abundance in sperm chromatin stability, especially regarding DNA damage and sperm head morphology alterations. Additionally, the study demonstrated the effectiveness of CMA3 as an alternative test for detecting PRM content in sperm, without the need for molecular-level assessments.
Article
Reproductive Biology
Jaime Gosalvez, Javier Bartolome-Nebreda, Eva Tvrda, Carmen Lopez-Fernandez, Jose Luis Fernandez, Stephen Johnston
Summary: The study aimed to investigate the relationship between DNase activity associated with bacterial contamination of incubated bovine frozen-thawed spermatozoa and elevated sperm DNA fragmentation. The experiments showed that incubation of plasmid with the supernatant of infected bovine semen straws resulted in clear evidence of DNase activity compared to non-infected supernatant. The presence of bacteria in semen straws led to an exponential increase in bacterial growth and sperm DNA fragmentation, highlighting the importance of hygiene practices during semen processing.
Article
Medicine, General & Internal
Liang Li, Shi bin Zhao
Summary: This study evaluated the clinical outcomes of testicular sperm versus ejaculated sperm in male patients with severe oligozoospermia undergoing intracytoplasmic sperm injection (ICSI). The results demonstrated that using testicular sperm for ICSI can improve biologic outcomes, especially for individuals with previous ICSI failures and male-partner oligozoospermia. This challenges the notion that ejaculated sperm is the preferred source for ICSI.
Article
Obstetrics & Gynecology
Sandro C. Esteve
Summary: The value of assessing subfertile males with oligozoospermia is controversial, but offering indiscriminate ICSI is not the best choice. Oligozoospermia is associated with poor male reproductive health, sperm damage, and adverse health consequences for offspring. Many testicular conditions can cause oligozoospermia, and therapeutic interventions can improve sperm quality and male health. Fertility clinics should collaborate with male infertility specialists to diagnose and treat oligozoospermia for better patient health, pregnancy prospects, and offspring well-being.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biochemistry & Molecular Biology
Daria Noto, Giulia Collodel, Daniela Cerretani, Cinzia Signorini, Laura Gambera, Andrea Menchiari, Elena Moretti
Summary: The study found that chlorogenic acid has antioxidant properties on human sperm, reducing damage caused by oxidative stress. Adding chlorogenic acid during handling and cryopreservation processes can improve sperm motility and DNA integrity.
Article
Agriculture, Dairy & Animal Science
Aya M. Fadl, Abdel Raouf M. Ghallab, Mostafa M. Abou-Ahmed, Adel R. Moawad
Summary: Supplementation of semen extender with 1.0 mM melatonin improved the quality of cooled and frozen/thawed rabbit spermatozoa, preserved DNA integrity, and enhanced fertility after artificial insemination.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Cell Biology
Sofia Makieva, Celine Staehli, Min Xie, Ana Velasco Gil, Maike Katja Sachs, Brigitte Leeners
Summary: This study aimed to determine the association between the time interval from insemination to vitrification and pregnancy rate. The findings suggest that the latest timing of vitrification is significantly associated with a higher pregnancy rate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rosetta Ponchia, Annunziata Bruno, Asia Renzi, Claudia Landi, Enxhi Shaba, Francesca Paola Luongo, Alesandro Haxhiu, Paolo Giovanni Artini, Alice Luddi, Laura Governini, Paola Piomboni
Summary: Despite the widespread use of sperm cryopreservation, it can cause detrimental alterations in sperm function, including decreased viability, motility, and DNA fragmentation. Oxidative stress appears to play a prominent role in the mechanisms of human sperm cryodamage. Antioxidants such as myo-inositol have been shown to minimize the detrimental effects of reactive oxygen species (ROS) and improve sperm quality and motility. This study demonstrated that post-thaw addition of myo-inositol significantly improved sperm parameters, increased oxygen consumption, and decreased carbonyl groups, indicating reduced oxidative stress. These findings suggest that the developed sperm cryopreservation procedure may improve current in vitro techniques used for sperm cryostorage in assisted reproductive technology (ART) laboratories.
Article
Agriculture, Dairy & Animal Science
Lingwei Sun, Mengqian He, Jiehuan Xu, Caifeng Wu, Shushan Zhang, Defu Zhang, Jianjun Dai, Jun Gao
Summary: The study aimed to evaluate the benefits of MitoQ in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes. The results showed that MitoQ at a concentration of 150 nM improved post-thawed sperm quality and motility by restoring ATP levels and preventing membrane damage. It also improved redox balance and exhibited antiapoptotic activity. These findings suggest that MitoQ can be an effective additive in rooster semen cryopreservation technology.
Review
Biology
Jordi Ribas-Maynou, Marc Yeste, Nerea Becerra-Tomas, Kenneth Aston, Emma R. James, Albert Salas-Huetos
Summary: A systematic review and meta-analysis of studies on IVF and ICSI treatments revealed a significant negative impact of sperm DNA damage on implantation and pregnancy rates. Routine sperm DNA testing is justified to improve IVF outcomes and assist in treatment selection for couples. Further studies are needed to draw more precise conclusions, especially in the context of ICSI treatments.
BIOLOGICAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Pingyu Sun, Guoyu Zhang, Ming Xian, Guangzhi Zhang, Fei Wen, Zhangtao Hu, Jianhong Hu
Summary: This study utilized 4D-DIA quantitative proteomics analysis to identify differentially expressed proteins (DEPs) in frozen-thawed dairy goat spermatozoa, which may influence the sperm's freezing ability. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed the association of these DEPs with the regulation of biological processes, metabolic processes, response to stress, and cellular component biogenesis. These findings provide insights into the underlying processes of proteome alterations and sperm freezing resistance, contributing to further research on sperm cryopreservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Brenda Florencia Gimeno, Maria Victoria Bariani, Lucia Laiz-Quiroga, Eduardo Martinez-Leon, Micaela Von-Meyeren, Osvaldo Rey, Adrian Angel Mutto, Claudia Elena Osycka-Salut
Summary: This study improved the yield of fertilization-capable sperm from equine frozen sperm by optimizing manipulation conditions and establishing a primary culture model of oviductal cells. The in vitro model demonstrated the potential fertilizing capacity of sperm released from oviductal cells, suggesting advancements in handling and selection of cryopreserved sperm for improved efficiencies in assisted reproductive techniques.
Article
Agriculture, Dairy & Animal Science
Kampon Kaeoket, Panida Chanapiwat
Summary: Resveratrol at an optimal concentration enhances the quality of cryopreserved boar sperm, but a high concentration has detrimental effects.
Article
Biology
Akshatha Daddangadi, Shubhashree Uppangala, Guruprasad Kalthur, Riccardo Talevi, Satish Kumar Adiga
Article
Environmental Sciences
Arpitha Rao, Guruprasad Nayak, Sandhya Kumari, Sneha Guruprasad Kalthur, Sadhana P. Mutalik, Srinivas Mutalik, Satish Kumar Adiga, Guruprasad Kalthur
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2020)
Article
Genetics & Heredity
Guruprasad Nayak, Sujith Raj Salian, Pooja Agarwal, Pooja Suresh Poojary, Arpitha Rao, Sandhya Kumari, Sneha Guruprasad Kalthur, Ajjappla B. Shreya, Srinivas Mutalik, Satish Kumar Adiga, Guruprasad Kalthur
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Shweta Hegde, Keerthana Karunakar Poojary, Rhea Rasquinha, Daphne Norma Crasta, Divya Gopalan, Srinivas Mutalik, Sazada Siddiqui, Satish Kumar Adiga, Guruprasad Kalthur
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2020)
Article
Reproductive Biology
Shubhashree Uppangala, Gail Fernandes, Sujith Raj Salian, Pratap Kumar, Riccardo Talevi, Guruprasad Kalthur, Satish Kumar Adiga
REPRODUCTIVE BIOLOGY
(2020)
Article
Oncology
Prathima Tholeti, Shubhashree Uppangala, Vasudeva Bhat, Karthik S. Udupa, Vijay Kumar, Shobhana Patted, Pandiyan Natarajan, Norah Spears, Guruprasad Kalthur, Teresa K. Woodruff, Satish Kumar Adiga
Summary: This study aimed to understand the knowledge, attitudes, and barriers of health care providers in oncofertility across India. Results showed that oncologists and gynecologists had varying levels of awareness of ASCO FP guidelines and different practices in oncofertility. Effective collaboration between these two groups is essential to overcome barriers and establish successful fertility preservation programs.
JOURNAL OF ADOLESCENT AND YOUNG ADULT ONCOLOGY
(2021)
Article
Genetics & Heredity
M. Salama, L. Ataman-Millhouse, M. Braham, K. Berjeb, M. Khrouf, J. K. Rodrigues, F. M. Reis, T. Cury-Silva, F. Sanchez, S. Romero, J. Smitz, L. Vasquez, M. Vega, F. Sobral, G. Terrado, M. G. Lombardi, A. Scarella, M. T. Bourlon, H. Verduzco-Aguirre, A. M. Sanchez, S. K. Adiga, P. Tholeti, K. S. Udupa, N. Mahajan, M. Patil, R. Dalvi, C. Venter, G. Demetriou, J. Geel, R. Quintana, G. Rodriguez, T. Quintana, L. Viale, M. Fraguglia, M. Coirini, Y. A. Remolina-Bonilla, J. A. R. Noguera, J. C. Velasquez, A. Suarez, G. D. Arango, J. I. D. Pineda, M. D. C. Aldecoa, M. Javed, H. Al Sufyan, N. Daniels, B. C. Oranye, A. A. Ogunmokun, K. I. Onwuzurigbo, C. J. Okereke, T. C. Whesu, T. K. Woodruff
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2020)
Article
Multidisciplinary Sciences
Sujith Raj Salian, Shubhashree Uppangala, Aswathi Cheredath, Fiona D'Souza, Guruprasad Kalthur, Vinod C. Nayak, Richard A. Anderson, Satish Kumar Adiga
Article
Plant Sciences
Guruprasad Nayak, Arpitha Rao, Prashansha Mullick, Srinivas Mutalik, Sneha Guruprasad Kalthur, Satish Kumar Adiga, Guruprasad Kalthur
JOURNAL OF ETHNOPHARMACOLOGY
(2020)
Meeting Abstract
Obstetrics & Gynecology
R. Talevi, G. Catapano, M. Di Nardo, V. Costanzo, V. Barbato, M. Ruggiero, A. Ferraro, S. Adiga, R. Gualtieri
HUMAN REPRODUCTION
(2019)
Article
Oncology
Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C. M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Claudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sanchez, Guillermo Terrado, Jhenifer K. Rodrigues, Joao Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Ligia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, Maria del Mar Andres, Maria T. Bourlon, Mario Vega, Mauricio Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)
Article
Oncology
Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C. M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Claudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sanchez, Guillermo Terrado, Jhenifer K. Rodrigues, Joao Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Ligia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, Maria del Mar Andres, Maria T. Bourlon, Mario Vega, Mauricio Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)
Article
Oncology
Mahmoud Salama, Lauren Ataman-Millhouse, Fabio Sobral, Guillermo Terrado, Anibal Scarella, Maria T. Bourlon, Satish Kumar Adiga, Karthik S. Udupa, Nalini Mahajan, Madhuri Patil, Chris Venter, Georgia Demetriou, Ramiro Quintana, Gabriela Rodriguez, Tomas Quintana, Luz Viale, Yuly Andrea Remolina Bonilla, July Andrea Russi Noguera, Juan Carlos Velasquez Velasquez, Jennifer Ivonne Dominguez Pineda, Mario Daniel Castro Aldecoa, Murid Javed, Hamad Al Sufyan, Nonso Daniels, Adegbite A. Ogunmokun, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)
Article
Oncology
Lauren M. Ataman, Jhenifer K. Rodrigues, Ricardo M. Marinho, Joao P. J. Caetano, Mauricio B. Chehin, Eduardo L. Alves da Motta, Paulo Serafini, Nao Suzuki, Tatsuro Furui, Seido Takae, Yodo Sugishita, Ken-Ichiro Morishige, Teresa Almeida-Santos, Claudia Melo, Karen Buzaglo, Kate Irwin, W. Hamish Wallace, Richard A. Anderson, Roderick T. Mitchell, Evelyn E. Telfer, Satish K. Adiga, Antoinette Anazodo, Catharyn Stern, Elizabeth Sullivan, Yasmin Jayasinghe, Lisa Orme, Richard Cohn, Rob McLachlan, Rebecca Deans, Franca Agresta, Brigitte Gerstl, William L. Ledger, Rebecca L. Robker, Joao M. de Meneses e Silva, Ligia H. F. Melo e Silva, Franciele O. Lunardi, Jung R. Lee, Chang S. Suh, Michael De Vos, Ellen Van Moer, Dominic Stoop, Veerle Vloeberghs, Johan Smitz, Herman Tournaye, Ludwig Wildt, Katharina Winkler-Crepaz, Claus Y. Andersen, Brigid M. Smith, Kristin Smith, Teresa K. Woodruff
JCO GLOBAL ONCOLOGY
(2020)
