Review
Endocrinology & Metabolism
Mausumi Das, Weon-Young Son
Summary: In vitro maturation (IVM) is a viable option for specific patients, including those at risk of ovarian hyperstimulation syndrome (OHSS), in need of urgent fertility preservation, or when controlled ovarian stimulation is not possible. The clinical applications of IVM are expanding rapidly and it is now classified as a non-experimental technique. IVM offers advantages over conventional IVF, such as reduced stimulation and risk of OHSS, shorter treatment times, and lower costs. However, challenges remain in terms of technical expertise and lower success rates compared to traditional IVF. Ongoing research aims to refine protocols and improve the efficiency and effectiveness of IVM.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Evelyn E. Telfer, Claus Yding Andersen
Summary: The cryopreservation of ovarian tissue is widely used to preserve fertility in high-risk females. Besides transplantation, in vitro maturation of oocytes from ovarian tissue can also be used to restore fertility safely in cases where transplantation is contraindicated.
FERTILITY AND STERILITY
(2021)
Review
Medicine, General & Internal
Chloe De Roo, Kelly Tilleman
Summary: In vitro maturation of ovarian tissue oocytes is an effective assisted reproductive treatment, but further validation and research are needed for its effectiveness. The introduction of biphasic IVM (CAPA-IVM) has shown significant improvement in the maturation protocol for oocytes.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ahmed Atef Mesalam, Marwa El-Sheikh, Myeong-Don Joo, Atif Ali Khan Khalil, Ayman Mesalam, Mi-Jeong Ahn, Il-Keun Kong
Summary: Exposing bovine oocytes to juglone at concentrations of 12.5-50.0 μM significantly impairs their development by inducing ROS accumulation, apoptosis, and mitochondrial dysfunction. This study provides insights into the adverse effects of juglone treatment on oocyte maturation and embryo development, highlighting the importance of understanding the impact of traditional medicinal components on reproductive processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Desmond A. R. Tutt, Gizem Guven-Ates, Wing Yee Kwong, Rob Simmons, Fei Sang, Giuseppe Silvestri, Carla Canedo-Ribeiro, Alan H. Handyside, Remi Labrecque, Marc-Andre Sirard, Richard D. Emes, Darren K. Griffin, Kevin D. Sinclair
Summary: Removing complex proteins during bovine IVM affects the metabolism of cumulus cells. The addition of melatonin is beneficial in the presence of complex proteins but detrimental in their absence. These findings lay the foundation for developing chemically defined IVM systems for the generation of cytogenetically and epigenetically normal embryos.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Gemma Fabozzi, Laura Albricci, Danilo Cimadomo, Maria Giulia Amendola, Federica Sanges, Roberta Maggiulli, Filippo Maria Ubaldi, Laura Rienzi
Summary: A study on sibling oocytes from women aged 42 years or younger undergoing ICSI cycles with ejaculated spermatozoa compared blastulation rates of a novel Geri-medium and a continuous single culture medium (CSCM), showing similar rates but differences in blastocyst morphology and developmental timings. Euploidy and ongoing implantation rates were similar between the two media, suggesting further research on the observed differences is needed.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Biochemistry & Molecular Biology
Nunzia Limatola, Jong Tai Chun, Kazuyoshi Chiba, Luigia Santella
Summary: Immature starfish oocytes are susceptible to polyspermy but can be activated by one sperm after the resumption of the meiotic cycle. DTT treatment can induce oocyte maturation but impairs the sperm-induced Ca2+ response.
Article
Agriculture, Dairy & Animal Science
Jose Felipe Warmling Spricigo, Ana Luiza Silva Guimaraes, Andrielle Thainar Mendes Cunha, Ligiane de Oliveira Leme, Marcos Coura Carneiro, Mauricio Machaim Franco, Margot Alves Nunes Dode
Summary: This study aimed to evaluate the potential to predict the quality of oocytes by measuring the transcript abundance of a panel of candidate genes in immature and/or mature cumulus cells. The results showed that none of the selected genes can accurately predict the potential of oocytes to develop into embryos. Additionally, cumulus cell biopsy negatively affects embryo development, blastocyst cell number, and apoptotic cell ratio.
Review
Obstetrics & Gynecology
Ri-Cheng Chian, Jian-Hua Li, Jin-Ho Lim, Hiroaki Yoshida
Summary: This article summarizes the clinical application of in vitro maturation (IVM) of immature oocytes based on published literature. Successful pregnancy rates with IVM technology are correlated with the number of immature oocytes obtained. Improvement in IVM medium itself has not achieved significant breakthroughs, but the clinical application of IVM technology continues to evolve.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, Pouya Dini
Summary: This study aimed to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. The results showed an association between oocyte maturation and pathways relating to nuclear maturation in oocytes and GTPase activity, extracellular matrix organization, and collagen trimers in cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Seon Ah Ryu, Song Baek, Keun Cheon Kim, Eun Song Lee, Seung Tae Lee
Summary: The presence of cumulus cells is not essential for the completion of cytoplasmic maturation in oocytes after nuclear maturation. Removal of cumulus cells from porcine medium antral follicles (MAFs)-derived cumulus-oocyte complexes (COCs) after nuclear maturation results in significant improvements in cytoplasmic maturation and preimplantation development. The cumulus cells do not play a relevant role in cytoplasmic maturation of immature oocytes.
Article
Obstetrics & Gynecology
Molly M. Quinn, Philip Marsh, Salustiano Ribeiro, Rhodel K. Simbulan, Cristina Hickman, Jorgen Berntsen, Mitchell P. Rosen
Summary: Embryo quality, morphokinetic parameters, and aneuploidy rates were found to be similar in sibling embryos cultured in distinct media systems from the time of gamete isolation.
HUMAN REPRODUCTION
(2022)
Article
Medicine, General & Internal
Xia Hao, Jessie Phoon, Lina Barbunopulos, Mona Sheikhi, Arturo Reyes Palomares, Kenny A. Rodriguez-Wallberg
Summary: The study investigated the developmental potential of mature metaphase II (MII) human oocytes obtained from germinal vesicle oocytes (GVs) after rescue in vitro maturation (rIVM) and the impact of vitrification at different timepoints. Results showed that the likelihood of obtaining MII oocytes was slightly higher in the fresh rIVM group compared to the vitrification-warming-rIVM group. However, the fertilization and developmental rates were reduced in both groups that underwent vitrification either before or after rIVM.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, Research & Experimental
Jianhua Li, Jing Chen, Tiecheng Sun, Shuiwen Zhang, Tingting Jiao, Ri-Cheng Chian, Youzhu Li, Ye Xu
Summary: In vitro oocyte maturation (IVM) does not affect the quality of embryos and the aneuploidy rate of embryos. The aneuploidy rate of embryos increases with maternal age, especially over 35 years old, whether in IVF or IVM group. Embryos with poor quality, especially grade III embryos, are recommended to be tested by PGS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Genetics & Heredity
S. E. Pors, D. Nikiforov, J. Cadenas, Z. Ghezelayagh, Y. Wakimoto, L. A. Z. Jara, J. Cheng, M. Dueholm, K. T. Macklon, E. M. Flachs, L. S. Mamsen, S. G. Kristensen, C. Yding Andersen
Summary: This study aimed to investigate the impact of oocyte diameter and cumulus cell mass on the potential for final maturation of immature human oocytes in vitro. The results showed that the oocyte diameter was positively associated with a higher incidence of MII, and the size of the cumulus cell mass was significantly associated with a larger oocyte diameter and the MII stage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)