Article
Cell Biology
Han-Yu Wang, Yi-Ru Shen, Yung-Chieh Tsai, Shang-Rung Wu, Chia-Yih Wang, Pao-Lin Kuo
Summary: SEPT12 and SEPT4 proteins are involved in maintaining the sperm annulus and regulating sperm capacitation. The phosphorylation status of SEPT12 and the activity of SEPT4 together control the expression and localization of sAC protein, which is crucial for sperm activation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jana Rychtarova, Alena Langerova, Helena Fulka, Pasqualino Loi, Michal Benc, Josef Fulka
Summary: The long-term storage of biological material is crucial for various fields, and a simple approach for evaluating sperm DNA damage level has been proposed to address the issue of limited frozen biological material quantities.
Article
Environmental Sciences
Wafaa H. Mohamed, Marwa F. Ali, Doha Yahia, Hassan A. Hussein
Summary: This study evaluated the potential reproductive toxicity of sulfoxaflor (SFX) insecticide in male Sprague Dawley rats. The results showed that SFX exposure led to abnormal sperm morphology, sperm DNA damage, and significant pathological changes in the testes, epididymis, and seminal vesicles of the rats.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Joel R. Drevet, Jorge Hallak, Mohammad-Hossein Nasr-Esfahani, Robert J. Aitken
Summary: This review explores the relationship between spermatozoa and reactive oxygen species (ROS), highlighting how spermatozoa rely on ROS for maturity but are highly susceptible to oxidative damage. Recent studies have shown the significant impact of oxidative damage on sperm structures and functions, particularly in terms of fertilization and embryonic development. Future research aims to investigate whether pro-oxidative situations can lead to epigenetic changes in spermatozoa.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Entomology
Hiroki Sakai, Takeshi Yokoyama, Shuichiro Tomita
Summary: New tissue observation methods have enabled researchers to capture morphological and histological features that have not been previously observed in silkworm eggs. The study emphasized the effectiveness of the CUBIC method in making silkworm eggs transparent and observing the behavior of various nuclei during fertilization.
JOURNAL OF INSECT PHYSIOLOGY
(2022)
Article
Obstetrics & Gynecology
Weijian Zhu, Lei Jiang, Chengshuang Pan, Junhui Sun, Xuefeng Huang, Wuhua Ni
Summary: The study evaluated DNA methylation patterns in sperm from men with differential levels of sperm DNA fragmentation index (DFI). The results showed that high DFI in male sperm was associated with significantly altered methylation in specific CpG fragments corresponding to nine genes. This suggests that sperm DNA damage may influence DNA methylation patterns in human sperm.
FERTILITY AND STERILITY
(2021)
Review
Agriculture, Dairy & Animal Science
Mohsen Sharafi, Seyyed Mohsen Borghei-Rad, Maryam Hezavehei, Abdolhossein Shahverdi, James D. Benson
Summary: This comprehensive review examines the mechanisms of damage caused by cryopreservation and highlights classical and novel strategies to reduce this damage and improve sperm survival. Cryopreservation is an important method for preserving germplasm in various fields, but the current methods only yield about 50% viable spermatozoa after cryopreservation. Various sperm components, including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, and microRNAs, can be damaged during the process. Conventional strategies using chemical additives have been employed, but they are not completely effective in preventing damage.
Article
Cell Biology
Judith Mueller, Monique Furlan, David Settele, Benjamin Grupp, Nils Johnsson
Summary: Muller et al. have discovered the role of septin sumoylation in cell separation. Sumoylated septins recruit the Fir1-Skt5 complex, which stimulates chitin synthase III for septum formation. This study sheds light on the significance of septin sumoylation in cell separation, which was previously unclear.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Fisheries
Victor Mauricio Medina-Robles, Leydy Yasmin Sandoval-Vargas, Roger Oswaldo Suarez-Martinez, Edwin Gomez-Ramirez, Diana Nataly Guaje-Ramirez, Pablo Emilio Cruz-Casallas
Summary: Little attention has been given to the effects of long-term cryopreservation on sperm quality in Piaractus orinoquensis. This study examined the effects of cryopreservation for different periods of time on various sperm parameters in this species. The results showed a decrease in sperm motility, viability, DNA integrity, and ATP content after 6 months of cryopreservation. The total antioxidant capacity did not significantly differ between fresh and cryopreserved sperm. Cryopreserved samples also exhibited an increase in sperm abnormalities compared to fresh sperm, with short tail being the most common defect. Ultrastructural analysis revealed damage to the plasma membrane, mitochondria, nucleus, and mid-region of post-thaw spermatozoa.
AQUACULTURE REPORTS
(2023)
Review
Cell Biology
Giulia Russo, Michael Krauss
Summary: Cytokinesis involves the final separation of a mother cell into two daughter cells, with septins playing a crucial role in this process. Septins are recruited to the cleavage furrow early on and undergo constant rearrangement during cytokinetic progression, ultimately concentrating within the intercellular bridge. This concentration of septins at specific sites may support cytokinetic progression by interacting with actin, microtubules, and associated motors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nutrition & Dietetics
Jorge Briseno-Bugarin, Isabel Hernandez-Ochoa, Xelha Araujo-Padilla, Maria Angelica Mojica-Villegas, Ricardo Ivan Montano-Gonzalez, Gabriela Gutierrez-Salmean, German Chamorro-Cevallos
Summary: Our study demonstrated that PBP crude extract at 200 mg/kg mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP, indicating the potential of PBP extract as a protective agent against CP toxicity.
Editorial Material
Obstetrics & Gynecology
Sheena E. M. Lewis
Summary: Varicocele is a common cause of male infertility, but it can be treated. Varicoceles can harm semen and sperm DNA quality, leading to lower pregnancy and live birth rates. Urologists are playing a more prominent role in infertility treatment, and men are becoming more aware of their own fertility and seeking equal care. There is a better understanding of the mechanisms of varicocele-induced damage, and repair surgery can improve semen and sperm DNA quality, increasing pregnancy and live birth rates.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Review
Endocrinology & Metabolism
Massimo Romano, Federico Cirillo, Daria Spadaro, Andrea Busnelli, Stefano Castellano, Elena Albani, Paolo Emanuele Levi-Setti
Summary: Infertility affects 10% to 15% of couples worldwide, with male factor being responsible for 50% of cases. Oral supplementation with antioxidants may improve sperm quality by reducing oxidative damage. The effectiveness of using testicular sperm in infertile men with high DFI is still inconclusive, and further large-scale studies are needed.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jordi Ribas-Maynou, Ariadna Delgado-Bermudez, Yentel Mateo-Otero, Estel Vinolas, Carlos O. Hidalgo, W. Steven Ward, Marc Yeste
Summary: This study analyzed cryopreserved bovine sperm after thawing and found that DNA damage and sperm degradation are influenced by oxidative stress. Evaluating chromatin integrity can help predict bull fertility.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Lilli Bittner-Schwerda, Carolina Herrera, Sarah Wyck, Eleni Malama, Christine Wrenzycki, Heinrich Bollwein
Summary: Oxidatively stressed sperm can negatively impact embryo development when fertilizing oocytes, and the oocyte's ability to handle this damage plays a significant role in the outcome. By using Brilliant Cresyl Blue (BCB) staining to differentiate oocytes of different qualities, this study found that BCB-negative oocytes had lower embryo development rates and lower blastocyst quality when fertilized with oxidatively stressed sperm. These findings can help in selecting high-quality oocytes for assisted reproduction involving damaged sperm, while also highlighting the importance of oocyte repair and antioxidative capacity in limiting oxidative damage to the progeny.
Article
Biochemistry & Molecular Biology
Ya-Yun Wang, Han-Sun Chiang, Chiao-Yin Cheng, Yi-No Wu, Yung-Chih Lin, Hsuan-Che Liu, Wei-Kung Tsai, Yen-Lin Chen, Ying-Hung Lin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Urology & Nephrology
Yi-No Wu, Kuo-Chiang Chen, Chun-Hou Liao, Han-Sun Chiang
Article
Clinical Neurology
H. -W. Kan, H. Chiang, W. -M. Lin, I. -S. Yu, S. -W. Lin, S. -T. Hsieh
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2018)
Article
Plant Sciences
Sung Hsin-Ching, Liu Chen-Wei, Hsiao Chien-Yu, Lin Shu-Rung, Yu I-Shing, Lin Shu-Wha, Chiang Ming-Hsien, Liang Chan-Jung, Pu Chi-Ming, Chen Yu-Chen, Lin Ming-Shian, Chen Yuh-Lien
Article
Multidisciplinary Sciences
Chun-Kai Chang, Xiu-Ru Lin, Yen-Lin Lin, Woei-Horng Fang, Shu-Wha Lin, Sui-Yuan Chang, Jau-Tsuen Kao
Article
Biochemistry & Molecular Biology
Chia-Yen Huang, Ya-Yun Wang, Ying-Liang Chen, Mei-Feng Chen, Han-Sun Chiang, Pao-Lin Kuo, Ying-Hung Lin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Chung-Hsin Yeh, Ya-Yun Wang, Shi-Kae Wee, Mei-Feng Chen, Han-Sun Chiang, Pao-Lin Kuo, Ying-Hung Lin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biotechnology & Applied Microbiology
Yi-No Wu, Kuo-Chiang Chen, Chien-Chih Wu, Ying-Hung Lin, Han-Sun Chiang
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Medicine, General & Internal
Ya-Yun Wang, Tsung-Hsuan Lai, Mei-Feng Chen, Hui-Ling Lee, Pao-Lin Kuo, Ying-Hung Lin
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Genetics & Heredity
Ya-Yun Wang, Chih-Chun Ke, Yen-Lin Chen, Yu-Hua Lin, I-Shing Yu, Wei-Chi Ku, Moira K. O'Bryan, Ying-Hung Lin
Article
Biochemistry & Molecular Biology
Yu-Hua Lin, Chia-Yen Huang, Chih-Chun Ke, Ya-Yun Wang, Tsung-Hsuan Lai, Hsuan-Che Liu, Wei-Chi Ku, Chying-Chyuan Chan, Ying-Hung Lin
Article
Biochemistry & Molecular Biology
Yu-Hsiang Lin, Chi-Chung Wang, Ying-Hung Lin, Bing-Huei Chen
Summary: Catechin nanoemulsions prepared from Oolong tea leaf waste showed high stability and anti-cancer activity on prostate cancer cells DU-145 and DU-145-induced tumors in mice, with significant inhibitory effects on cell growth and induction of apoptosis, as well as reduction of tumor volume and weight through a decrease in EGF and VEGF levels in serum.
Article
Biochemistry & Molecular Biology
Ya-Yun Wang, Ying-Hung Lin, Vin-Cent Wu, Yu-Hua Lin, Chia-Yen Huang, Wei-Chi Ku, Chiao-Yin Sun
Summary: Oxidative stress is a major cause of male infertility, and the protein Klotho is involved in oxidative regulation and male fertility. This study shows that Klotho deficiency accelerates damage to sperm quality and spermatogenesis, and affects the expression of GST proteins which are important for antioxidant defense.
Article
Medicine, General & Internal
Yieh-Loong Tsai, Tsung-Hsuan Lai, Hsuan-Che Liu, Ya-Yun Wang, Yu-Hua Lin, Chih-Chun Ke, Ming-Ting Chung, Chying-Chyuan Chan, Ying-Hung Lin
Summary: This study found that RAB3C is more abundant than RAB3A, 3B, and 3D in human testicular tissues. During human spermatogenesis, RAB3C protein is mainly enriched in elongated spermatids, and in mature human spermatozoa, it is concentrated in the postacrosomal region, neck, and midpiece. The delocalization of RAB3C signals was observed in human spermatozoa with SEPT14 mutation, along with defective head and tail.
MEDICINA-LITHUANIA
(2022)
Meeting Abstract
Urology & Nephrology
ChunHou Liao, YiNo Wu, HanSun Chiang
JOURNAL OF UROLOGY
(2018)
