Article
Cell Biology
Yeon Sun Kim, Seung Chel Yang, Mira Park, Youngsok Choi, Francesco J. DeMayo, John P. Lydon, Hye-Ryun Kim, Hyunjung Jade Lim, Haengseok Song
Summary: This study analyzed the phenotypes of Dgcr8 conditional knockout mice in the female reproductive tract, revealing distinct reproductive defects in Dgcr8(td/td) mice. The deficiency of Dgcr8 with different Cre systems induced overlapping but distinct phenotypes and altered the profiles of microRNAs and their target mRNAs, highlighting the importance of selecting appropriate Cre drivers for gene investigation.
CELL PROLIFERATION
(2021)
Article
Immunology
Guan Wang, Cunling Zhang, Hiroto Kambara, Cheryl Dambrot, Xuemei Xie, Li Zhao, Rong Xu, Andrea Oneglia, Fei Liu, Hongbo R. Luo
Summary: This study used TLA sequencing to determine the integration site and flanking sequences of the MRP8-Cre-ires/EGFP transgene, revealing its integration into chromosome 5 of the host mouse genome, leading to a deletion of host genomic sequence. The researchers designed a new genotyping protocol, but found that crossing heterozygous mice did not produce homozygous mice, likely due to disrupted Ap1s1 gene expression resulting in prenatal lethality.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Prashanth Anamthathmakula, Philemon D. Shallie, Neha Nayak, Sabita Dhal, Jay L. Vivian, Gil Mor, Michael J. Soares, Nihar R. Nayak
Summary: The Cyp19-Cre transgenic mouse model was used for placenta-specific gene inactivation, but unexpected phenotypes were observed. Further analysis revealed heterogeneous recombinase activity in the placenta, which was influenced by parental inheritance. Therefore, extensive characterization and careful interpretation of placental phenotypes are necessary when using the Cyp19-Cre model for placenta-specific mutagenesis studies.
Article
Biochemistry & Molecular Biology
Irena Krueger, Friedrich Reusswig, Kim Juergen Krott, Celina Fabienne Lersch, Martina Spelleken, Margitta Elvers
Summary: The study demonstrates that the mT/mG Cre reporter mouse is a suitable model for real-time visualization of platelets, analysis of cell morphology, and identification of non-recombined platelets. However, a small proportion of leukocytes exhibit mG fluorescence in PF4-Cre positive mice. Therefore, careful evaluation of platelet analysis beyond hemostasis and thrombosis should be conducted when increasing recombination of immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Y. Kitase, M. Prideaux
Summary: This article summarizes the main methods for studying the functions of bone cells, including gene knockout and cell tracing. However, there are concerns about the specificity of the promoters used and the off-target effects on cells within and outside of the bone. A thorough understanding of when and where these promoters are activated will improve the quality of study design and the confidence in data interpretation.
Article
Biochemistry & Molecular Biology
Alexander A. Dolskiy, Andrey S. Gudymo, Oleg S. Taranov, Irina V. Grishchenko, Ekaterina M. Shitik, Dmitry Yu Prokopov, Vladislav O. Soldatov, Elvira V. Sobolevskaya, Sergey A. Bodnev, Natalia V. Danilchenko, Anastasia A. Moiseeva, Polina Y. Torzhkova, Yulia A. Bulanovich, Galina S. Onhonova, Elena K. Ivleva, Marina V. Kubekina, Andrey E. Belykh, Tatiana V. Tregubchak, Alexander B. Ryzhikov, Elena V. Gavrilova, Rinat A. Maksyutov, Alexey V. Deykin, Dmitry V. Yudkin
Summary: COVID-19 has become a socially significant infection and the researchers have developed a new transgenic mouse model with human ACE2 gene expression for studying SARS-CoV-2 infection. The study found that the virus had the highest viral load in the lungs, brain, heart, and intestine. This model provides a useful tool for COVID-19 research.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Caroline Moser, Leon G. Straub, Yael Rachamin, Christian Wolfrum
Summary: This protocol outlines a method to assess adipocyte numbers within a specific depot based on inducible genomic labeling, allowing for quantification of white, brown, and brite/beige adipocytes in a size-independent manner. The system is highly sensitive and spatially unbiased, providing detailed information for research purposes.
Article
Genetics & Heredity
Kara Corps, Monica Stanwick, Juliann Rectenwald, Andrew Kruggel, Sarah B. Peters
Summary: The loss of TGF beta receptor II in mice resulted in bone and teeth defects, as well as osteochondral dysplasia throughout the skeleton, potentially leading to breathing difficulties and malnutrition. These abnormalities were primarily caused by skeletal deformities, affecting the mice's ability to sense and absorb food.
Article
Neurosciences
Xinzhu Yu, Stefanie L. Moye, Baljit S. Khakh
Summary: By attenuating astrocyte calcium signaling in vivo using specific mouse models, researchers observed significant behavioral alterations in adult mice. Additionally, this attenuation led to cellular and molecular changes, particularly in the cerebellum. These findings provide valuable insights into the role of astrocytes in neural circuits and behavior.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Plant Sciences
Xudong Ye, Zarir Vaghchhipawala, Edward J. Williams, Changlin Fu, Jinyuan Liu, Fengming Lu, Erin L. Hall, Shirley X. Guo, LaRee Frank, Larry A. Gilbertson
Summary: Efficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre recombinase expression. Different crops require diverse promoters to drive Cre expression, and the efficiency of marker-free progeny generation varies among different crops.
PLANT CELL REPORTS
(2023)
Article
Cell Biology
Yi-Chao Hsu, Yu-Fen Chung, Mei-Shu Chen, Chi-Kuang Wang, Si-Tse Jiang, Ing-Ming Chiu
Summary: The F1A-CreER(T2) mouse line, driven by the Fgf1A promoter, can be used for time-dependent and lineage tracing of Fgf1A-expressing cells in vivo.
Article
Biochemistry & Molecular Biology
Sumiyo Morita, Takuro Horii, Mika Kimura, Ryosuke Kobayashi, Hiroki Tanaka, Hidetaka Akita, Izuho Hatada
Summary: Knockout mice are important tools for studying the normal function of genes. Liver-specific conditional knockout mice can be generated using lipid nanoparticles, which offers a more convenient method compared to traditional approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Blanca Jimenez-Gomez, Patricia Ortega-Saenz, Lin Gao, Patricia Gonzalez-Rodriguez, Paula Garcia-Flores, Navdeep Chandel, Jose Lopez-Barneo
Summary: The hypoxic ventilatory response is triggered by chemoreceptor glomus cells in the carotid body that are connected to the respiratory center in the brainstem. The function of mitochondrial complex I (MCI) in acute oxygen sensing by glomus cells is still unclear. This study shows that transgenic expression of the yeast enzyme NDI1, which does not directly contribute to proton pumping, can fully restore the hypoxic ventilatory response and sensitivity to hypoxia in MCI-deficient mice. These findings highlight the necessity of maintaining mitochondrial NADH dehydrogenase activity for oxygen-dependent regulation of breathing and demonstrate the potential of gene therapy for functional recovery.
NATURE COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Robert L. Kruse, Mercedes Barzi, Xavier Legras, Francis P. Pankowicz, Nika Furey, Lan Liao, Janming Xu, Beatrice Bissig-Choisat, Betty L. Slagle, Karl-Dimiter Bissig
Summary: This study developed a novel transgenic mouse model that allows for excision of HBV from mouse chromosomes to generate a recombinant circular DNA molecule. The model showed regulation of HBV expression and demonstrated the potential for HBV clearance.
Article
Geriatrics & Gerontology
Laurie K. Bale, Sally A. West, Cheryl A. Conover
Summary: The study examined the effects of brain-specific PAPP-A KO in mice on metabolism and lifespan, revealing improvements in lifespan markers in non-specific PAPP-A KO mice with thymic involution. However, brain-specific PAPP-A KO mice showed no significant differences compared to the control group. Thorough characterization of tissue-specific mouse models and consideration of potential germline recombination are crucial for accurate data interpretation.
EXPERIMENTAL GERONTOLOGY
(2021)
Letter
Biophysics
Seiichiro Katagiri, Tomohiro Umezu, Kenko Azuma, Chiaki Kobayashi, Daigo Akahane, Tamiko Suguro, Nahoko Furuya, Hiroaki Fujimoto, Naoya Nakamura, Junko H. Ohyashiki, Kazuma Ohyashiki
BONE MARROW TRANSPLANTATION
(2019)
Article
Oncology
Yoshikazu Ito, Tomohiro Umezu, Kenichi Tadokoro, Yuu Saito, Seiichiro Katagiri, Tamiko Suguro, Michiyo Asano, Seiichiro Yoshizawa, Daigo Akahane, Yuko Tanaka, Hiroaki Fujimoto, Seiichi Okabe, Moritaka Gotoh, Tetsuzo Tauchi, Chiaki Kawana, Junko H. Ohyashiki, Naoya Nakamura, Kazuma Ohyashiki
LEUKEMIA & LYMPHOMA
(2019)
Article
Multidisciplinary Sciences
Sho Kubota, Kenji Tokunaga, Tomohiro Umezu, Takako Yokomizo-Nakano, Yuqi Sun, Motohiko Oshima, Kar Ton Tan, Henry Yang, Akinori Kanai, Eisaku Iwanaga, Norio Asou, Takahiro Maeda, Naomi Nakagata, Atsushi Iwama, Kazuma Ohyashiki, Motomi Osato, Goro Sashida
NATURE COMMUNICATIONS
(2019)
Letter
Hematology
Seiichiro Katagiri, Hideki Makishima, Kenko Azuma, Yasuhito Nannya, Yuu Saitoh, Seiichiro Yoshizawa, Daigo Akahane, Hiroaki Fujimoto, Yoshikazu Ito, Ravi Velaga, Tomohiro Umezu, Junko H. Ohyashiki, Seishi Ogawa, Kazuma Ohyashiki
Article
Neurosciences
Leonie S. Brebner, Joseph J. Ziminski, Gabriella Margetts-Smith, Meike C. Sieburg, Hayley M. Reeve, Thomas Nowotny, Johannes Hirrlinger, Tristan G. Heintz, Leon Lagnado, Shigeki Kato, Kazuto Kobayashi, Leslie A. Ramsey, Catherine N. Hall, Hans S. Crombag, Eisuke Koya
JOURNAL OF NEUROSCIENCE
(2020)
Article
Oncology
Yuu Saitoh, Tomohiro Umezu, Satoshi Imanishi, Michiyo Asano, Seiichiro Yoshizawa, Seiichiro Katagiri, Tamiko Suguro, Hiroaki Fujimoto, Daigo Akahane, Chiaki Kobayashi-Kawana, Junko H. Ohyashiki, Kazuma Ohyashiki
Article
Cell Biology
Nina Gladkova, Tomohiro Umezu, Satoshi Imanishi, Chiaki Kawana, Junko H. Ohyashiki, Kazuma Ohyashiki
Article
Neurosciences
Ryoji Fukabori, Yoshio Iguchi, Shigeki Kato, Kazumi Takahashi, Satoshi Eifuku, Shingo Tsuji, Akihiro Hazama, Motokazu Uchigashima, Masahiko Watanabe, Hiroshi Mizuma, Yilong Cui, Hirotaka Onoe, Keigo Hikishima, Yasunobu Yasoshima, Makoto Osanai, Ryo Inagaki, Kohji Fukunaga, Takuma Nishijo, Toshihiko Momiyama, Richard Benton, Kazuto Kobayashi
JOURNAL OF NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Tomohiro Umezu, Koichi Tsuneyama, Kohsuke Kanekura, Michiyo Hayakawa, Toshihito Tanahashi, Mitsuoki Kawano, Y-h Taguchi, Hidenori Toyoda, Akihiro Tamori, Masahiko Kuroda, Yoshiki Murakami
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Hiroo Takahashi, Ryo Asahina, Masayuki Fujioka, Takeshi K. Matsui, Shigeki Kato, Eiichiro Mori, Hiroyuki Hioki, Tohru Yamamoto, Kazuto Kobayashi, Akio Tsuboi
Summary: The study demonstrates that the collaborative action of activity-dependent transcription factor Npas4 and Gem gene can promote neuroprotection, reduce neuronal cell death after stroke, and serve as a potential new drug target.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Yukiko Otsuka, Hitomi Tsuge, Shiori Uezono, Soshi Tanabe, Maki Fujiwara, Miki Miwa, Shigeki Kato, Katsuki Nakamura, Kazuto Kobayashi, Ken-ichi Inoue, Masahiko Takada
Summary: The NeuRet vector generally exhibits higher retrograde gene transfer efficiency compared to the HiRet vector, but its retrograde transgene expression in macaque brains is unexpectedly low. However, the NeuRet vector demonstrates higher neuron specificity and causes minimal inflammatory/immune responses in primate brains. The efficiency of retrograde gene transfer with the NeuRet vector varies depending on the species and input projections.
Article
Oncology
Kuno Suzuki, Hideyoshi Igata, Motoki Abe, Yusuke Yamamoto
Summary: Liquid biopsy is a promising cancer screening method due to its low invasiveness and ability to detect multiple types of cancer. Small RNAs can be used for cancer type classification in samples from a single facility, but interfacility biases can affect the classification of samples from multiple facilities.
Article
Multidisciplinary Sciences
Makoto Takemoto, Shigeki Kato, Kazuto Kobayashi, Wen-Jie Song
Summary: This study reveals that there are two distinct neuronal subpopulations in layer 5 of the mouse dysgranular insula, namely L5a and L5b. L5a cells project bilaterally to the lateral and capsular divisions of the central amygdala, while L5b cells project ipsilaterally to the parasubthalamic nucleus and the medial division of the central amygdala. Activation of L5a and L5b cells leads to suppressed and facilitated water drinking behavior in thirsty mice, respectively.
Article
Medicine, General & Internal
Masaki Asakage, Yoshihiko Usui, Naoya Nezu, Hiroyuki Shimizu, Kinya Tsubota, Kazuhiko Umazume, Naoyuki Yamakawa, Tomohiro Umezu, Hirotsugu Suwanai, Masahiko Kuroda, Hiroshi Goto
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Hematology
Tomohiro Umezu, Satoshi Imanishi, Seiichiro Yoshizawa, Chiaki Kawana, Junko H. Ohyashiki, Kazuma Ohyashiki
