Article
Medicine, General & Internal
Amr Moubasher, Tarek Abdel-Raheem, Hossam Ahmed, Ahmed Salem, Alpesh Doshi, Amr Abdel Raheem
Summary: This study compared the outcomes of intracytoplasmic morphologically selected sperm injection (IMSI) and intracytoplasmic sperm injection (ICSI) in vitro fertilization (IVF) procedures, and found that ICSI had statistically better results in terms of chemical pregnancy, clinical pregnancy, live birth, and fertilization rates compared to IMSI. Although there were no significant differences in miscarriage rates, ICSI showed significant advantages in certain subgroups regarding chemical and clinical pregnancy rates.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Genetics & Heredity
Jiaxiong Wang, Hui Tang, Qinyan Zou, Aiyan Zheng, Hong Li, Shenmin Yang, Jingjing Xiang
Summary: This study identified a homozygous pathogenic CATSPER3 mutation in a male infertility patient from a consanguineous family, revealing its impact on assisted reproductive technology outcome. The mutation caused failure of sperm acrosome reaction without affecting routine semen parameters. Successful treatment was achieved through ICSI.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2021)
Review
Medicine, General & Internal
Sandrine Chamayou, Filippo Giacone, Rossella Cannarella, Antonino Guglielmino
Summary: The intracytoplasmic sperm injection (ICSI) technique was invented to address severe male infertility caused by altered sperm parameters. It is now widely used worldwide for couple infertility treatment. ICSI can be performed using any available spermatozoon, regardless of motility, morphology, or quantity. Published data shows that ICSI accelerates zygote kinetics and leads to higher fertilization rates and more blastocysts for clinical use compared to conventional IVF. Various sperm and spermatozoa characteristics, such as parameters, morphology, vitality, DNA content, RNA content, epigenetics, and recovery site, have different impacts on fertilization, blastocyst rates, and embryo kinetics. While ICSI is the most common solution for couple infertility, understanding the causes of male infertility is crucial for producing competent spermatozoa that contribute to normal embryonic development and healthy offspring.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Reproductive Biology
Luisa Ramirez-Agamez, Camilo Hernandez-Aviles, Dickson D. Varner, Charles C. Love
Summary: This study explores stallion-associated factors in intracytoplasmic sperm injection (ICSI) using frozen/thawed sperm. The results indicate that the composition of freezing extender affects the developmental competence of sperm and in vitro-matured equine oocytes after ICSI. Furthermore, there were no significant differences observed when using ejaculated or epididymal sperm, or sperm with different morphological features in ICSI outcomes.
Article
Medicine, General & Internal
Krzysztof Lukaszuk, Grzegorz Jakiel, Izabela Woclawek Potocka, Jolanta Kiewisz, Jolanta Olszewska, Wlodzimierz Sieg, Amira Podolak, Ewa Pastuszek, Artur Wdowiak
Summary: ICSI is a commonly used treatment for oocyte fertilization, and the quality of sperm selection depends on accurate visualization of morphology. We emphasize the importance of optical system's resolution in observing sperm and provide specific microscope system setup recommendations. Furthermore, a lack of standardization in microscopy techniques for both ICSI and IMSI may contribute to mixed research results.
Article
Obstetrics & Gynecology
Ahmad Aboukhshaba, Nahid Punjani, Sofia Doukakis, Nikica Zaninovic, Gianpiero Palermo, Peter N. Schlegel
Summary: The study aimed to evaluate the impact of sperm characteristics on intracytoplasmic sperm injection (ICSI) outcomes in men with nonobstructive azoospermia (NOA) following microdissection testicular sperm extraction (mTESE). The results showed that specific sperm defects did not completely preclude the chance of clinical pregnancy. This study is the first comprehensive evaluation of the importance of these characteristics in ICSI outcomes.
FERTILITY AND STERILITY
(2022)
Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Sara Ruiz-Diaz, Rosane Mazzarella, Paula Navarrete-Lopez, Raul Fernandez-Gonzalez, Celia de Frutos, Maria Maroto, Claudia Cucala, Paula Beltran-Brena, Marta Lombo, Dimitrios Rizos, Alfonso Gutierrez-Adan
Summary: This study aimed to analyze if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. The results showed that thermotactic sperm selection led to higher DNA integrity, small and elongated head size parameters, and a different sperm subpopulation structure.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Review
Obstetrics & Gynecology
Nami Morishita, Masanori Ochi, Toshitaka Horiuchi
Summary: This study discussed the advantages of using Syrian hamsters in reproductive biology and developmental biology research, as well as the challenges of in vitro embryo manipulations due to their sensitivity to environmental factors. The authors summarized key points for hamster ICSI, highlighting the importance of controlled environmental conditions, timing of sperm injection, and optimal culture conditions. Despite low success rates, further research is needed to improve manipulation techniques for hamster ICSI and to address experimental challenges.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2021)
Review
Andrology
Kareim Khalafalla, Ahmad Majzoub, Haitham Elbardisi, Ashni Bhathella, Arth Chaudhari, Ashok Agarwal, Ralf Henkel, Thoraya AlMarzooki, Hasan Burjaq, Mohamed Arafa
Summary: This study aimed to assess the impact of different levels of sperm DNA fragmentation on the outcome of clinical intracytoplasmic sperm injection. Results showed that in cases where sperm DNA fragmentation exceeded 30%, favorable females with older age or lower anti-Mullerian hormone levels had significantly higher clinical pregnancy and live birth rates compared to unfavorable females.
Article
Andrology
Ping Ping, Zhong Zheng, Yi Ma, Sha-Sha Zou, Xiang-Feng Chen
Summary: This study evaluated the impact of different spermatogenic conditions on the outcomes of intracytoplasmic sperm injection (ICSI) and the risk of major birth defects. The results showed that nonobstructive azoospermia (NOA) had a negative effect on fertilization rate, but did not affect other embryological and clinical outcomes. Spermatogenic failure and sperm origin did not affect the clinical outcomes of ICSI treatment.
ASIAN JOURNAL OF ANDROLOGY
(2022)
Review
Medicine, General & Internal
Lauren Lacey, Sibte Hassan, Sebastian Franik, Mourad W. Seif, M. Ahsan Akhtar
Summary: The effects of assisted hatching (AH) on live birth rates are uncertain and may increase the risk of multiple pregnancies. Although AH may offer a slightly increased chance of achieving a clinical pregnancy, the quality of data is low, and it is uncertain whether it influences miscarriage rates.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Genetics & Heredity
Meghan B. Smith, Jacqueline R. Ho, Victoria Cortessis, Irene Jiyao Chen, Kristin A. Bendikson, Richard J. Paulson, Lynda K. McGinnis, Ali Ahmady
Summary: In good prognosis couples, there was no difference in fertilization or blastulation rates based on timing of ICSI within the currently accepted 2- to 6-hour window post-retrieval based on a 34-hour trigger. The oocyte has a physiological tolerance for fertilization during this time window, and variations in ICSI timing within this window are unlikely to impact cycle outcome.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Adva Aizer, Alon Lazarovich, Olga Dratviman-Storobinsky, Meirav Noach-Hirsh, Jigal Haas, Avi Jacob, Gil Raviv, Raoul Orvieto
Summary: The study found that in ICSI cycles, using motile spermatozoa obtained from TESA or TESE resulted in higher pregnancy and live birth rates compared to using immotile spermatozoa. While fertilization rates and embryo quality decreased with the use of immotile spermatozoa, ICSI remained a valid option for couples.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Review
Genetics & Heredity
Veena Unnikrishnan, John Kastelic, Jacob Thundathil
Summary: In cattle, the efficiency of ICSI is hindered by failed oocyte activation post sperm injection. Research focusing on proteins like Na/K-ATPase and PLC zeta is key to improving the success rate. Enhancing the efficiency of bovine ICSI has significant implications for the cattle breeding industry.
