Review
Medicine, General & Internal
Eric Han, David B. Seifer
Summary: Oocyte cryopreservation (OC) is a process of stimulating ovarian follicles, retrieving follicular fluid, isolating and vitrifying mature oocytes. It has been increasingly used as an option for future biologic children for patients undergoing gonadotoxic therapies. Planned OC, also known as elective OC, is growing in popularity to overcome age-related fertility decline. This review discusses medically indicated and planned OC, focusing on the physiology, technique, timing, financial considerations, and outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Ghina Ghazeeri, Christine Beyrouthy, Lina El-taha, May Abiad, Duaa Fahs
Summary: This study aims to assess Lebanese women's awareness and acceptance of egg freezing and found that factors such as age, marital status, and fertility affect women's attitudes towards the technology. Medical egg freezing is widely accepted and the main indication for social egg freezing is to assist women who struggle to find a suitable partner during their peak fertile years. Concerns about safety and future fertility are among the factors that limit women's decision to undergo the procedure.
Article
Obstetrics & Gynecology
Marta Venturas, Xingbo Yang, Kishlay Kumar, Dagan Wells, Catherine Racowsky, Daniel J. Needleman
Summary: Fluorescence lifetime imaging microscopy (FLIM) can accurately measure the metabolic state of cumulus cell clusters, with parameters significantly correlated with patient age and AMH levels but not with BMI. This technique may have potential value in identifying superior oocytes.
FERTILITY AND STERILITY
(2021)
Article
Reproductive Biology
Monica De los Reyes, Jaime Palomino, Allison Villagra, George Ramirez, Oscar A. Peralta, Victor H. Parraguez, Karla Aspee
Summary: This study aimed to evaluate the effect of progesterone on meiotic development related to the gene expression of connexin 37 and connexin 43 in canine cumulus oocyte complexes. The results showed that progesterone increased meiotic development in canine oocytes but had no effect on the gene expression of connexin 37 and connexin 43.
Article
Reproductive Biology
Maria Florencia Suqueli Garcia, Nadia Gabbanelli, Glenda Laura Rios, Jorgelina Buschiazzo
Summary: This study found that progesterone (P4) can induce acrosome exocytosis in sperm without compromising sperm viability or activating sperm caspases. Furthermore, using lower concentrations of exogenous P4 during in vitro fertilization (IVF) can enhance the developmental competence of partially cumulus-denuded oocytes.
Article
Nanoscience & Nanotechnology
Akalabya Bissoyi, Ruben M. F. Tomas, Yanan Gao, Qiongyu Guo, Matthew I. Gibson
Summary: This study successfully cryopreserved hepatocyte spheroids using macromolecular cryoprotectants added to DMSO solutions, which significantly improved post-thaw recovery and minimized cryo-injury related membrane damage. Adding polyampholytes also rescued reduced actin polymerization, potentially linked to intracellular ice formation. This research may facilitate access to off-the-shelf and ready-to-use frozen spheroids, reducing the need for in-house culturing and the number of in vivo experiments.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Obstetrics & Gynecology
A. Tsafrir, H. Holzer, T. Miron-Shatz, T. Eldar-Geva, M. Gal, I Ben-ami, N. Dekel, A. Weintruab, D. Goldberg, O. Schonberger, N. Srebnik, J. Hyman
Summary: Most women who underwent planned oocyte cryopreservation did so because they didn't want to become pregnant without a partner. While the majority of those who attempted to conceive were successful, some eventually chose to use donor spermatozoa for conception.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Review
Chemistry, Analytical
Yu Fang, Ruige Wu, Joo Mong Lee, Ling Hou Melinda Chan, Kok Yen Jerry Chan
Summary: The field of assisted reproductive technology (ART) has been constantly improving, and microfluidic technology has the potential to play a transformative role in optimizing embryo development. Microfluidics can be integrated into various stages of embryo production, enabling miniaturization, integration, and automation. Progress in 3D printing technology, organ-on-a-chip, alternative materials, and integrated microfluidic operational units will drive the development of this technology. An ideal microfluidic system would incorporate oocyte processing, sperm processing, and embryo culture into a single compact device. This article reviews the recent applications of microfluidic technologies in IVF procedures and discusses the challenges and potentials for their application in ART.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Euihyun Lee, Carlos R. Baiz
Summary: This study characterizes the interactions between DMSO and water, showing that DMSO disrupts the hydrogen bond networks and this disruption increases with decreasing temperature.
Article
Obstetrics & Gynecology
Jennifer K. Blakemore, James A. Grifo, Shannon M. DeVore, Brooke Hodes-Wertz, Alan S. Berkeley
Summary: This study evaluated the outcomes of patients undergoing planned oocyte cryopreservation, finding that 38.1% of patients took action with their cryopreserved oocytes, while 58.9% did not use them. Further research with larger cohorts and epidemiological comparisons to current egg freezing patients are needed.
FERTILITY AND STERILITY
(2021)
Article
Agriculture, Dairy & Animal Science
Ishani Dinisri, Sandarenu Kodikara, Madhusha Prasadani, Indunil Pathirana, Chathura Rathnayake, Basil Alexander, Kai-Fai Lee, Suranga Pradeep Kodithuwakku
Summary: Mancozeb negatively impacts caprine oocytes and granulosa cells, potentially impairing fertilization ability and developmental potential of the oocytes, and suppressing ovulation. It suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation in oocytes, while also inducing progesterone secretion from granulosa cells.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Daniel Angel-Velez, Tine De Coster, Nima Azari-Dolatabad, Andrea Fernandez-Montoro, Camilla Benedetti, Osvaldo Bogado Pascottini, Henri Woelders, Ann Van Soom, Katrien Smits
Summary: This study optimized a vitrification protocol for equine oocytes by comparing different cryoprotective agents. A promising combination of cryoprotectants consisting of propylene glycol, ethylene glycol, and galactose was identified, showing blastocyst rates similar to fresh oocytes. Further optimization is needed for clinical efficiency in equine oocyte vitrification.
Article
Obstetrics & Gynecology
Eva M. E. Balkenende, Taghride Dahhan, Catharina C. M. Beerendonk, Kathrin Fleischer, Dominic Stoop, Annelies M. E. Bos, Cornelis B. Lambalk, Roel Schats, Jesper M. J. Smeenk, Leonie A. Louwe, Astrid E. P. Cantineau, Jan Peter de Bruin, Sabine C. Linn, Fulco van der Veen, Madelon van Wely, Mariette Goddijn
Summary: Alternative ovarian stimulation protocols with tamoxifen or letrozole did not affect the number of cumulus-oocyte complexes (COCs) retrieved at follicle aspiration in women with breast cancer.
HUMAN REPRODUCTION
(2022)
Article
Developmental Biology
Kaitlin R. Karl, Peter Z. Schall, Zaramasina L. Clark, Meghan L. Ruebel, Jose Cibelli, Robert J. Tempelman, Keith E. Latham, James J. Ireland
Summary: Excessive FSH doses during ovarian stimulation in heifers with small ovarian reserve can cause disruptions in cumulus cell and oocyte functions, leading to premature cumulus expansion and follicular hyperstimulation dysgenesis. These effects can impair the quality of cumulus-oocyte complexes and hinder successful fertilization.
MOLECULAR HUMAN REPRODUCTION
(2023)