Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.
Article
Biochemistry & Molecular Biology
Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, Pouya Dini
Summary: This study aimed to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. The results showed an association between oocyte maturation and pathways relating to nuclear maturation in oocytes and GTPase activity, extracellular matrix organization, and collagen trimers in cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yue Han, Xinglin Qu, Xuan Chen, Yanqiu Lv, Yuyang Zhang, Yi Jin
Summary: This study investigates the effect of porcine follicular fluid exosomes on cumulus expansion and oocyte maturation. The results suggest that porcine FF-Exo play a role in oocyte nuclear maturation but do not significantly affect cumulus expansion or mitochondrial membrane potential.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Seon Ah Ryu, Song Baek, Keun Cheon Kim, Eun Song Lee, Seung Tae Lee
Summary: The presence of cumulus cells is not essential for the completion of cytoplasmic maturation in oocytes after nuclear maturation. Removal of cumulus cells from porcine medium antral follicles (MAFs)-derived cumulus-oocyte complexes (COCs) after nuclear maturation results in significant improvements in cytoplasmic maturation and preimplantation development. The cumulus cells do not play a relevant role in cytoplasmic maturation of immature oocytes.
Article
Obstetrics & Gynecology
Robert B. Hood, Donghai Liang, Youran Tan, Jennifer B. Ford, Irene Souter, Jorge E. Chavarro, Dean P. Jones, Russ Hauser, Audrey J. Gaskins
Summary: This study identified the metabolic pathways and metabolites in the serum and follicular fluid that are associated with peak estradiol levels and the number of mature oocytes. The serum metabolome mainly involves fatty acid and amino acid pathways, while the follicular fluid metabolome mainly involves lipid, vitamin, and hormone pathways. These findings provide insights into the biological mechanisms underlying follicular and oocyte development.
HUMAN REPRODUCTION
(2023)
Article
Endocrinology & Metabolism
Chong Wang, Xiaoyang Fei, Hongyan Zhang, Wenjing Zhou, Zhaojun Cheng, Ying Feng
Summary: This study identified differentially expressed proteins in mature and immature oocytes using iTRAQ labelling and 2D LC-MS/MS. ACPP and CD5L were found to be differentially expressed between MII and Gv oocytes. Further measurements revealed significantly lower level of ACPP in the ovarian follicular fluid and cumulus cells of DOR patients, indicating poor oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biology
Jihong Yang, Yangbai Li, Suying Li, Yan Zhang, Ruizhi Feng, Rui Huang, Minjian Chen, Yun Qian
Summary: A two-stage metabolomic analysis reveals the characteristics of human follicular fluid and predictors of follicular development, with lysophosphatidylcholine (LPC) proposed as a biomarker for follicular development. This study provides valuable insights into metabolic regulation during follicular development and offers potential detection and therapeutic targets for improving human follicle and oocyte health.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anne-Sophie Neyroud, Regina Maria Chiechio, Gregory Moulin, Solene Ducarre, Claire Heichette, Aurelien Dupont, Mathieu Budzynski, Pascale Even-Hernandez, Maria Jose Lo Faro, Marina Yefimova, Valerie Marchi, Celia Ravel
Summary: The morphology and quantification of extracellular vesicles (EVs) in follicular fluid (FF) of healthy individuals were studied in this research. EVs play important roles in the development of female gametes, but limited knowledge exists regarding their morphology. The findings of this study provide a comprehensive morphological description and quantification of FF-derived EVs, serving as a reference for further research on EVs in ovarian diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Euihyun Kim, Kihae Ra, Myung-Shin Lee, Geon A. Kim
Summary: Exosomes derived from porcine follicular fluid can improve the morphology and functionality of pig oocytes when added to the maturation medium. Therefore, it is suggested to use FF-derived exosomes in controlled experiments in embryology to enhance the reliability of experimental data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
A. C. A. Ferreira, N. A. R. Sa, R. F. Silva, F. G. C. Sousa, J. C. Anjos, J. Cadenas, B. G. Alves, L. F. Lima, J. J. H. Celestino, M. O. Gastal, A. P. R. Rodrigues, E. L. Gastal, J. R. Figueiredo
Summary: This study compared the morphological features, estradiol production, and gene expression between in vitro-derived and in vivo-derived early antral caprine follicles. The results showed that in vitro-derived antral follicles cultured in a specific medium had comparable oocyte maturation rates to in vivo-derived early antral follicles.
Article
Genetics & Heredity
S. E. Pors, D. Nikiforov, J. Cadenas, Z. Ghezelayagh, Y. Wakimoto, L. A. Z. Jara, J. Cheng, M. Dueholm, K. T. Macklon, E. M. Flachs, L. S. Mamsen, S. G. Kristensen, C. Yding Andersen
Summary: This study aimed to investigate the impact of oocyte diameter and cumulus cell mass on the potential for final maturation of immature human oocytes in vitro. The results showed that the oocyte diameter was positively associated with a higher incidence of MII, and the size of the cumulus cell mass was significantly associated with a larger oocyte diameter and the MII stage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Fuhua Xu, Maralee S. Lawson, Yukie Bean, Alison Y. Ting, Tanja Pejovic, Koen De Geest, Melissa Moffitt, Shoukhrat M. Mitalipov, Jing Xu
Summary: Stage-specific AMH modulation in group culture promotes coordinated growth and function of human follicles, leading to mature oocytes. This method has been previously successful in nonhuman primates.
HUMAN REPRODUCTION
(2021)
Review
Endocrinology & Metabolism
Mausumi Das, Weon-Young Son
Summary: In vitro maturation (IVM) is a viable option for specific patients, including those at risk of ovarian hyperstimulation syndrome (OHSS), in need of urgent fertility preservation, or when controlled ovarian stimulation is not possible. The clinical applications of IVM are expanding rapidly and it is now classified as a non-experimental technique. IVM offers advantages over conventional IVF, such as reduced stimulation and risk of OHSS, shorter treatment times, and lower costs. However, challenges remain in terms of technical expertise and lower success rates compared to traditional IVF. Ongoing research aims to refine protocols and improve the efficiency and effectiveness of IVM.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Chang Liu, Meng Wang, Haixia Yao, Mengge Cui, Xueqi Gong, Lan Wang, Cong Sui, Hanwang Zhang
Summary: The study found that follicular extracellular vesicles (EVs) of polycystic ovary syndrome (PCOS) patients interfere with the quality of oocytes, leading to abnormal development and maturation. PCOS-EVs cause abnormal distribution of mitochondria and spindles in oocytes, inhibiting oocyte maturation. Moreover, oxidative stress induced by PCOS-EVs may be a potential cause.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Di Zhang, Jia Lv, Rongxin Tang, Ying Feng, Yongfeng Zhao, Xiaoyang Fei, Richeng Chian, Qigui Xie
Summary: The study investigated exosomal microRNAs in human ovarian follicular fluid to explore their potential association with oocyte quality. Analysis revealed that several exosomal miRNAs were critical for maintaining oocyte quality, suggesting they may be potential biomarkers for evaluating oocyte quality, which is important in clinical settings.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)