Article
Biology
Santosh Kumar, Chandramohan Chitraju, Robert V. Farese, Tobias C. Walther, Christopher G. Burd
Summary: Phosphatidylserine decarboxylase plays a crucial role in lipid droplet biogenesis and neutral lipid storage, with different forms being regulated by nutritional state. The enzyme can localize to either lipid droplets or mitochondria based on growth conditions, affecting triacylglycerol synthesis when cells are challenged with free fatty acid.
Article
Cell Biology
Filomena Broeskamp, Elizabeth S. M. Edrich, Oskar Knittelfelder, Lisa Neuhaus, Thorsten Meyer, Jonas Heyden, Lukas Habernig, Florian Kreppel, Campbell W. Gourlay, Patrick Rockenfeller
Summary: This study investigated the impact of mitochondrial outer membrane protein Por1 on yeast autophagy, showing that POR1 deficiency reduces autophagic capacity and alters organelle and lipid homeostasis. It was found that the reduced autophagy caused by POR1 deficiency could be circumvented by overexpression of Psd1, suggesting a role for Por1 in Psd1-mediated autophagy regulation.
Article
Biotechnology & Applied Microbiology
Keiko Takagi, Akari Kikkawa, Ryo Iwama, Ryouichi Fukuda, Hiroyuki Horiuchi
Summary: Phosphatidylserine decarboxylases (PSDs) play a crucial role in maintaining phosphatidylethanolamine (PE) levels in fungi, with type II PSDs being unique to fungi and plants and important for fungal growth and morphogenesis. Deletion of psdB in Aspergillus nidulans resulted in severe growth defects, reduced conidiation efficiency, and abnormal conidiophore structures, highlighting the significant role of type II PSDs in phospholipid homeostasis and fungal development.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biology
Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai
Summary: In this study, highly glycosylated IsPETase enzyme was obtained by controlling the glycosylation modification of PETase in Pichia pastoris, improving the enzyme's specific activity and thermostability. The partially deglycosylated IsPETase still maintained high thermostability and could completely degrade PET flakes at 50 degrees C. This study provides a strategy to modulate the thermostable IsPETase through glycosylation engineering, promoting PET biodegradation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fidan Erden-Karaoglan, Mert Karaoglan
Summary: This study investigated the applicability of heterologous yeast promoters in Pichia pastoris, and found inducible and constitutive regulation modes as well as the optimal carbon sources for these promoters. The study also determined the DNA region with the highest activity for all promoters through promoter deletion analysis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Zuzana Pakanova, Marek Nemcovic, Peter Barath, Giovanni Musci
Summary: Ceruloplasmin is crucial for iron metabolism in vertebrates, and the production of high-quality recombinant human ceruloplasmin represents a significant advancement. Further improvement in productivity and glycoengineering is necessary for potential therapeutic use in aceruloplasminemia patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Shuai Wang, Yuanyuan Wang, Qingyan Yuan, Liu Yang, Fengguang Zhao, Ying Lin, Shuangyan Han
Summary: This study successfully obtained high methanol-tolerant Pichia pastoris chassis strains using iterative adaptive laboratory evolution (microbial microdroplet culture (MMC) and shake flask culture (SFC)), and clarified the mechanism of tolerance. The evolved strain exhibited good stress homeostasis at high methanol concentrations, which is largely dependent on regulating membrane lipid metabolism. The increased level of phosphatidylcholine and overexpression of the phosphatidylethanolamine N-methyltransferase 2 gene were found to play important roles in enhancing methanol tolerance.
Article
Biochemistry & Molecular Biology
Tianpeng Chen, Shimeng Wang, Huanqing Niu, Guanjia Yang, Sinan Wang, Yuqi Wang, Chaowei Zhou, Bin Yu, Pengpeng Yang, Wenjun Sun, Dong Liu, Hanjie Ying, Yong Chen
Summary: A biofilm-based fermentation system was constructed in P. pastoris by combining the surface display of beta-galactosidase and biofilm formation. The results showed a catalytic conversion rate of 50.3% for GOS with a maximum enzyme activity of 5125 U/g. This study provides a basis for developing biofilm-based surface display methods in P. pastoris.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Nai-Xin Lin, Rui-Zhen He, Yan Xu, Xiao-Wei Yu
Summary: The robust P. pastoris mutant demonstrates higher expression levels of not only lipase but also other heterogeneous proteins under methanol induction conditions. The mutant shows lower intracellular ROS levels compared to the WT. RNA-seq analysis revealed upregulation of key pathways related to antioxidant, MAPK pathway, ergosterol synthesis pathway, transcription factors, and the peroxisome pathway in the robust mutant, contributing to its enhanced oxidative stress tolerance and high heterologous protein expression efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Lina Heistinger, Juliane C. Dohm, Barbara G. Paes, Daniel Koizar, Christina Troyer, Oezge Ata, Teresa Steininger-Mairinger, Diethard Mattanovich
Summary: By characterizing 25 natural isolates from all seven described Komagataella species, this study identified strains with differences in tolerance to high pH, high temperature, and growth on xylose. The genotypic and phenotypic diversity within this genus provides a basis for utilizing neglected Komagataella strains with interesting characteristics and understanding genetic determinants for improved growth and stress tolerance.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Yasmin Nabilah Binti Mohd Fauzee, Yuki Yoshida, Yukio Kimata
Summary: In eukaryotic species, dysfunction of the endoplasmic reticulum (ER) triggers a cellular protective response called the unfolded protein response (UPR). This response is initiated by ER stress sensors, including Ire1, which splices and matures mRNA encoding the transcription factor Hac1. Through analyzing the methylotrophic yeast Pichia pastoris, researchers discovered a previously unknown function of Ire1. They found that Ire1 not only regulates UPR but also controls protein aggregation and the heat shock response, conferring heat stress resistance to the cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Yoshiki Eto, Narcis Saubi, Pau Ferrer, Joan Joseph-Munne
Summary: This study established a method for producing chimeric HPV-HIV L1P18 protein in Pichia pastoris, achieving VLPs with a purity of 96% using a combination of purification techniques. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV.
Article
Biochemistry & Molecular Biology
Mirelle Flores-Villegas, Corinna Rebnegger, Viktoria Kowarz, Roland Prielhofer, Diethard Mattanovich, Brigitte Gasser
Summary: The promoter of the high-affinity glucose transporter Gth1 (PGTH1) was improved through random and rational engineering, resulting in stronger induction in glucose-limitation and enabling methanol-free production processes. Single point mutations and key promoter segments were identified, leading to a library of novel promoter variants with significantly higher activity. The study also discovered nucleotide positions and corresponding TFBS that distinguish between glycerol- and glucose-mediated repression of the native promoter.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Khadijah A. Altammar, Jonathan Guyang Ling, Hussein M. Al-Bajalan, Iuan-Sheau Chin, Mukram Mohamed Mackeen, Nor Muhammad Mahadi, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
Summary: Cutinases are hydrolytic enzymes that can degrade plant cutin. A new cutinase homolog, AnCUT3, has been identified and characterized in this study. The recombinant AnCUT3 exhibited optimal temperature and pH, as well as high catalytic efficiency. It also showed potential for degrading and modifying the surface of certain polymers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Microbiology
Lukas Bernauer, Astrid Radkohl, Leonie Gabriela Katharina Lehmayer, Anita Emmerstorfer-Augustin
Summary: K. phaffii is an important model organism for studying eukaryotic molecular cell biology, with the intriguing property of being able to efficiently assimilate methanol. Research topics on this yeast range from studying methanol assimilation, peroxisome biogenesis and pexophagy to other cellular processes such as protein secretion, lipid biosynthesis and cell wall biogenesis. Comparing data obtained from K. phaffii with other yeasts can elucidate major differences and highlight its big potential in fundamental research.
FRONTIERS IN MICROBIOLOGY
(2021)