Article
Agriculture, Dairy & Animal Science
Zhu Qiao, Jiaxin Chen, Qiaqia Zhou, Xin Wang, Yuanyuan Shan, Yanglei Yi, Bianfang Liu, Yuan Zhou, Xin Lu
Summary: Bacteriocin BM173 was successfully expressed and purified, showing broad-spectrum antibacterial activity, high thermal and pH stability, as well as efficacy in disrupting cell membrane integrity and inhibiting biofilm formation. These findings suggest that BM173 has promising potential as a preservative in the dairy industry.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemical Research Methods
Tong Xin, Yanan Lv, Haoran Chen, Linlin Li, Lijun Shen, Guangcun Shan, Xi Chen, Hua Han
Summary: This article proposes a new method for serial section registration using an unsupervised optical flow network to measure feature similarity and eliminate nonlinear deformation. The optical flow network is also used to estimate and compensate for cumulative registration error, enabling the reconstruction of biological tissue structures. Experimental results demonstrate that this method effectively improves the spatial continuity of serial sections and enhances the accuracy of registration and reconstruction for biological tissue structures.
Article
Multidisciplinary Sciences
Joana D'Arc S. Trindade, Celio Geraldo Freire-de-Lima, Suzana Corte-Real, Debora Decote-Ricardo, Marco Edilson Freire de Lima
Summary: The study examined the in vitro trypanocidal effects of the non-steroidal anti-inflammatory drug nimesulide on Chagas disease, highlighting its potential as an alternative treatment option.
Article
Chemistry, Physical
Junjie Zhang, Yulin Liu, Yabei Li, Tong Zhu, Jinbin Qiu, Feng Xu, Hua Zhang, Fei Li
Summary: Understanding the oxidative state and damage of neurons under heat stress is crucial for studying heat-related neuronal injury. In this study, a temperature-controlled scanning electrochemical microscopy platform was developed for in situ and quantitative monitoring of neuronal oxygen consumption, extracellular hydrogen peroxide levels, and cell membrane permeability under thermal microenvironment. The results contribute to a better understanding of the heat-related cellular injury mechanism.
Article
Biotechnology & Applied Microbiology
Yuanyuan Zhu, Qingqing Zhou, Ping Li, Qing Gu
Summary: This study focused on the purification, characterization, and mode of action of the bacteriocin Paracin 54 produced by Lactobacillus paracasei ZFM54. Paracin 54 exhibited broad-spectrum inhibitory activity, with a strong inhibitory effect on Staphylococci and stability in acidic pH range. The mode of action results showed that Paracin 54 damages the cell membrane and cell wall of Staphylococcus aureus, leading to bacterial death.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Ewelina Kluza, Thijs J. Beldman, Annelie Shami, Edwin R. Scholl, Tsveta S. Malinova, Anita E. Grootemaat, Nicole N. van der Wel, Isabel Goncalves, Stephan Huveneers, Willem J. M. Mulder, Esther Lutgens
Summary: The study reveals a spectrum of endothelial abnormalities associated with atherosclerosis, with early lesions and the shoulder region of advanced plaques showing more severe abnormalities while the endothelium in the advanced plaque body has normalized. Similar endothelial features were observed in human atherosclerotic plaques, accompanied by thrombotic areas.
Article
Biochemistry & Molecular Biology
Yasmin Neves Vieira Sabino, Katialaine Correa de Araujo Domingues, Harsh Mathur, Laura G. Gomez-Mascaraque, Gaetan Drouin, Antonio Martinez-Abad, Marcos Rogerio Totola, Lucas Magalhaes Abreu, Paul D. Cotter, Hilario Cuquetto Mantovani
Summary: This study demonstrates the potential of exopolysaccharides (EPS) produced by Bacillus spp. as anti-biofilm compounds against Staphylococcus aureus associated with bovine mastitis, offering potential applications in the treatment of this costly disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Li Wang, Changshuo Li, Jintao Li, Xiaofei Zhang, Xiaochen Li, Yiran Cui, Yang Xia, Yinqi Zhang, Shengcheng Mao, Yuan Ji, Wang Sheng, Xiaodong Han
Summary: Liquid-phase electron microscopy allows high-resolution observation of biological samples in their native liquid state, offering unique applications for studying cells and membrane proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Vivek K. Bajpai, InWha Park, Imran Khan, Fanar Hamad Alshammari, Pradeep Kumar, Lei Chen, MinKyun Na, Yun Suk Huh, Young-Kyu Han, Shruti Shukla
Summary: The protoberberine alkaloid THBA showed significant antioxidant potential and antimicrobial activity against Bacillus cereus, disrupting the membrane integrity and inhibiting biofilm formation. It also reduced ROS levels in fibroblast cells and decreased CFU counts in raw rice, suggesting its potential as a natural antioxidant and antimicrobial agent.
Article
Biochemical Research Methods
Chisato Takahashi, Mari Sato, Chikara Sato
Summary: Staphylococcus epidermidis, a gram-positive bacterium, plays an important role in biofilm formation and microvesicle secretion. This study successfully imaged the microvesicles secreted by Staphylococcus epidermidis and their integration into biofilms using atmospheric scanning electron microscopy (ASEM). The information obtained is expected to help elucidate the biological mechanism of biofilm formation and develop medicines against biofilms and associated infections.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Yawei Li, Li Wang, Yufeng He, Wei Zheng, Langhong Lou, Jian Zhang
Summary: The study found that as the temperature increases, the spacing of dislocation networks in the gamma/gamma' interface decreases, while the minimum creep rate increases non-linearly. This is due to the enhanced dislocation-climb in the interface and gamma'-cutting by a <010> superdislocations.
SCRIPTA MATERIALIA
(2022)
Article
Multidisciplinary Sciences
Marie Vancova, Radim Skoupy, Eva Durinova, Tomas Bily, Jana Nebesarova, Vladislav Krzyzanek, Ales Kolouch, Petr Horodysky
Summary: This study reports the first use of two live-cell imaging agents as cathodoluminescent probes. The approach allows for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope.
SCIENTIFIC REPORTS
(2022)
Article
Dermatology
D. Mirakovski, K. Damevska, V Simeonovski, S. Nikolovska, B. Boev, A. Petrov, T. Sijakova Ivanova, A. Zendelska, M. Hadzi-Nikolova, I Boev, G. Dimov, R. Darlenski, J. Kazandjieva, S. Damevska, M. Situm
Summary: This study investigated the particles adhered to human skin exposed to urban air pollution using SEM-EDX analysis. Various types of particles were found on the skin, including chlorides, organic particles, silicates, carbonates, and metal-rich particles. The results provide new insights into the link between air pollution and skin damage.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Chemistry, Analytical
Zuzanna Brozek-Mucha, Jolanta Was-Gubala
Summary: Shooting distance assessment is crucial in forensic investigation. This study examined the effects of contact and close-range shooting on various types of cotton textiles using a 9 mm Luger pistol. The findings show that both the appearance of the shooting pattern and the distribution of gunshot residue are influenced by the properties of the textiles.
Article
Physics, Applied
Carles Bosch, Joerg Lindenau, Alexandra Pacureanu, Christopher J. Peddie, Marta Majkut, Andrew C. Douglas, Raffaella Carzaniga, Alexander Rack, Lucy Collinson, Andreas T. Schaefer, Heiko Stegmann
Summary: Correlative multimodal imaging is a valuable method for investigating complex structural relations in life sciences. It requires establishing sample preparation workflows that are compatible with multiple imaging techniques. This can involve imaging a fluorescently labeled region of interest in a biological soft tissue sample with light microscopy before staining the specimen with heavy metals for higher resolution structural imaging.
APPLIED PHYSICS LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Patricia A. Howe, Randy Worobo, Gavin L. Sacks
AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE
(2018)
Article
Chemistry, Applied
N. Estiarte, A. Crespo-Sempere, S. Marin, A. J. Ramos, R. W. Worobo
Article
Food Science & Technology
Joshua E. Herskovitz, Randy W. Worobo, Julie M. Goddard
JOURNAL OF FOOD SCIENCE
(2019)
Review
Microbiology
Yifan Cheng, Guoping Feng, Carmen I. Moraru
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
G. Feng, Y. Cheng, R. W. Worobo, D. A. Borca-Tasciuc, C. Moraru
LETTERS IN APPLIED MICROBIOLOGY
(2019)
Article
Microbiology
Magdalena Pajor, Zirui Ray Xiong, Randy W. Worobo, Piotr Szweda
Article
Biotechnology & Applied Microbiology
Jonathan H. Sogin, Gabriela Lopez Velasco, Burcu Yordem, Cari K. Lingle, John M. David, Mario Cobo, Randy W. Worobo
Summary: The study implemented ATP testing and microbiological enumeration in a tofu production facility to improve cleaning practices and overall plant hygiene. The results showed a statistically lower proportion of swabs failing sanitary requirements for ATP, aerobic microorganisms, and lactic acid bacteria after targeted cleaning, but not for yeasts and molds. ATP swabs and microbiological enumeration had a 75.1% agreement on site hygiene, and product data indicated a decrease in microbial load post-implementation of practices.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Karolina Pelka, Olga Otlowska, Randy W. Worobo, Piotr Szweda
Summary: This study investigated the antimicrobial potential of ethanolic extracts of bee bread and bee pollen, finding that bee bread extracts showed higher inhibitory potential against certain strains of Staphylococcus aureus. The study also observed that there was no correlation between phenolic content and antimicrobial activity.
Article
Infectious Diseases
Karolina Pelka, Randy W. Worobo, Justyna Walkusz, Piotr Szweda
Summary: The study aimed to isolate and identify bacteria present in mature bee bread and dried bee pollen, with findings indicating they may be potential sources of bacteria producing antimicrobial agents and/or enzymes of particular industrial importance. Further research is needed to verify this hypothesis, but preliminary results are promising.
Article
Microbiology
Katerina Roth, Yadwinder Singh Rana, Devin Daeschel, Jasna Kovac, Randy Worobo, Abigail B. Snyder
Summary: Six thermo-acidophilic, spore-forming strains were isolated from juice products, representing three novel species of Alicyclobacillus. These strains can grow aerobically in acidic and moderate thermal conditions, with only two of them capable of producing guaiacol.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yupawadee Galasong, Jonathan H. Sogin, Randy W. Worobo
Summary: The demand for clean-label products has increased due to public health concerns regarding artificial preservatives in food and beverage. Natural glycolipids (NG), derived from Dacryopinax spathularia, have been shown to be a highly effective preservative against spoilage yeasts, Gram-positive bacteria, and molds. In this study, NG was added to acidified beverages and its inhibitory effects against major spoilage microorganisms were compared to sodium benzoate (SB) over a 12-week period. The results demonstrated that NG can effectively protect beverages from microbial spoilage, especially against lactic acid bacteria and yeasts, either alone or in combination with dimethyl dicarbonate (DMDC).
Article
Biotechnology & Applied Microbiology
Ahmer Bin Hafeez, Karolina Pelka, Kamila Buzun, Randy Worobo, Piotr Szweda
Summary: This study aimed to analyze the whole genome and assess the antimicrobial potential of bacterial isolates from honey in northern Poland. A total of 132 strains were obtained from three honey samples, and the antimicrobial activity of CFAM was used to select strains for detailed genomic investigation. Two isolates were classified as Bacillus paralicheniformis and one isolate as Bacillus subtilis, and all three isolates showed potential as producers of antimicrobial compounds.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Isaya Kijpatanasilp, Khursheed Ahmad Shiekh, Saeid Jafari, Randy W. Worobo, Kitipong Assatarakul
Summary: This study evaluated the efficacy of UV radiation and nisin, alone and in combination, in comparison with thermal pasteurization on the microbial inhibition kinetics and physicochemical properties of tangerine juice. UV-149.76 J/cm(2) + nisin (200 ppm) combination showed the highest reduction in spoilage and pathogenic microbes. UV and nisin had no significant effect on pH, TSS, and TA values, but showed notable differences in color analysis and antioxidant activity. UV radiation and nisin combination could be a viable alternative to heat pasteurization during cold storage of fruit juice.
Article
Chemistry, Applied
Rebecca M. Cheng, Jessie Usaga, Randy W. Worobo
Summary: The effects of juice pH, type of acidulant, and post-treatment refrigeration on the high-pressure processing (HPP) inactivation of Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in acid beverages were evaluated. The results showed that HPP can effectively inactivate Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in acid beverages, and the pH of the juice and post-treatment refrigeration have significant effects on the effectiveness of HPP.
FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
(2023)
Review
Microbiology
Abigail B. Snyder, Randy W. Worobo