Article
Environmental Sciences
Gyulim Park, Kwang Min Lee, Young Seok Lee, Yedam Kim, Chae Min Jeon, O-Mi Lee, Yu-Jin Kim, Hong-Joo Son
Summary: In this study, a rapid and eco-friendly method for the degradation and valorization of feather waste using keratinase-producing Pseudomonas geniculata H10 was investigated. The results revealed that strain H10 could completely degrade chicken feathers within 48 hours and produce high levels of amino acids. The keratinase exhibited optimal temperature and pH conditions, as well as stability and degradation capability for various insoluble keratins.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Microbiology
Nagarajan Siddharthan, Ramasamy Balagurunathan, Natarajan Hemalatha
Summary: In this study, a novel feather-degrading Geobacillus thermodenitrificans PS41 bacterial isolate was identified from poultry farm soil. The isolated bacterial isolates showed significant feather degradation with keratinase enzyme production, indicating their potential in biodegradation of feather waste.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yong Meng, Yin Tang, Xiuhong Zhang, Jin Wang, Zhengfu Zhou
Summary: Keratinase can degrade keratin and has potential applications. In this study, a keratinase DgoKerA was identified in Deinococcus gobiensis and its enzyme activity was improved through molecular modification.
APPLIED SCIENCES-BASEL
(2022)
Article
Environmental Sciences
Mouna Ben Elhoul, Nadia Zarai Jaouadi, Khelifa Bouacem, Fawzi Allala, Hatem Rekik, Sondes Mechri, Haifa Khemir Ezzine, Neila Miled, Bassem Jaouadi
Summary: The keratin-degrading bacterium Actinomadura viridilutea DZ50 secretes a keratinase (KERDZ) with potential industrial interest. Recombinant enzyme rKERDZ has been shown to completely degrade chicken feather and remove wool/hair from various hides, while preserving collagen structure. The enzymatic process reduces biological and chemical oxygen demands in leather processing and offers a greener alternative to traditional dehairing methods.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Yin Tang, Leizhou Guo, Mingming Zhao, Yuan Gui, Jiahui Han, Wei Lu, Qilin Dai, Shijie Jiang, Min Lin, Zhengfu Zhou, Jin Wang
Summary: A thermostable keratinase DgeKer was identified from a slightly thermophilic species, D. geothermalis, showing high activity and stability at 70 degrees C and pH 9. It has great efficiency in degrading feathers and could have potential applications in various biotechnological industries.
APPLIED SCIENCES-BASEL
(2021)
Article
Microbiology
Shuaiying Peng, Hanguang Li, Shuaiwen Zhang, Rong Zhang, Xin Cheng, Kuntai Li
Summary: A highly efficient feather-degrading bacterium, named JY-23, was isolated and found to degrade 92.95% of chicken feathers in 72 hours. The degradation mechanism was identified as a synergistic action of sulfitolysis and proteolysis. Additionally, a new keratinase (KerJY-23) belonging to the M4 metalloprotease family was identified in this study.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Isha Sharma, Kumar Pranaw, Hemant Soni, Hemant Kumar Rawat, Naveen Kango
Summary: This study evaluated the production of keratinase from a newly isolated B. velezensis NCIM 5802 and improved the production and feather degradation through optimization. The study also explored the biochemical properties of the keratinase and the keratin degradation mechanism through various analyses, confirming its effectiveness and multiple forms.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Doaa A. Goda, Ahmad R. Bassiouny, Nihad M. Abdel Monem, Nadia A. Soliman, Yasser R. Abdel-Fattah
Summary: The optimized parameters for incubating protein lysate from waste feathers using keratinase produced by Laceyella sacchari strain YNDH led to a significant increase in total protein content. The partially purified enzyme from this strain showed activity at high temperatures and pH levels, with characteristics suggesting a mixed serine-metallo enzyme type. The research also revealed the presence of essential and rare amino acids in the produced Feather Protein Lysate (FPL).
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Ruben Javier-Lopez, Edoardo Mandolini, Munavvara Dzhuraeva, Khursheda Bobodzhanova, Nils-Kare Birkeland
Summary: A new subspecies of Fervidobacterium pennivorans, named T, was discovered in a high-altitude hot spring in Tajikistan. Strain T is an obligate anaerobic rod that can degrade feather keratin at high temperatures, similar to other members of the Fervidobacterium genus. This study provides insights into the physiological and genomic properties of strain T, highlighting its unique keratinolytic ability.
Article
Biochemistry & Molecular Biology
Isha Sharma, Naveen Kango
Summary: This study successfully increased the efficiency of keratinase secretion by Ochrobactrum intermedium through optimizing process parameters, revealing that the enzyme exhibited high activity and stability under specific conditions. Additionally, calcium ions were found to enhance keratinase activity, while PMSF and EDTA were able to inhibit its activity, demonstrating high efficiency in degrading poultry feathers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Naikun Shen, Mengying Yang, Chenjie Xie, Jiangxin Pan, Kunrong Pang, Hongyan Zhang, Yibing Wang, Mingguo Jiang
Summary: A novel feather-degrading bacteria, Bacillus tropicus strain Gxun-17, was isolated from the soil sample of a marine duck farm in Beibu Gulf, Guangxi, China. The optimum fermentation conditions were determined as feather concentration of 15 g/L, maltose concentration of 10.0 g/L, MgSO4 concentration of 0.1 g/L, initial pH of 7.0 and temperature of 32.5°C. The strain completely degraded feathers within 48 h and exhibited a keratinase activity of 112.57 U/mL, which was 3.18-fold higher than that obtained with the basic medium. The keratinase activity showed optimal performance at pH 7.0 and a temperature of 60°C. The keratinase demonstrated high specificity towards the casein substrate, with Km and Vmax values of 15.24 mg/mL and 0.01 mg/(mL center dot min), respectively. Mn2+ and β-mercaptoethanol enhanced keratinase activity, while Mg2+, Ca2+, K+, Co2+, Al3+, phenylmethylsulphonyl fluoride and isopropanol inhibited it.
Article
Environmental Sciences
Zhongtao Sun, Xianyao Li, Kexin Liu, Xiaoli Chi, Liying Liu
Summary: This study isolated Bacillus pumilusJYL bacteria from soil sample, which can degrade feathers and promote plant growth, providing a potential method for utilizing feather waste as nitrogen fertilizer.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Microbiology
Nagwa M. Abd El-Aziz, Bigad E. Khalil, Hayam Fouad Ibrahim
Summary: In this study, actinomyces isolates from poultry farms were tested for their keratinase activity. The strain with the highest activity was identified as Streptomyces werraensis KN23 and mutants were generated through mutagenesis. The best mutant, SA-27, showed a keratinase activity of 106.92 U/ml. Optimization using response surface methodology resulted in a maximum activity of 129.60 U/ml. The genetic diversity of wild type and mutants was studied using ISSR, revealing two clusters with sub-clusters and sub-sub clusters.
Article
Biochemistry & Molecular Biology
Kuntai Li, Ganghui Li, Shuaiying Peng, Minghui Tan
Summary: This study focused on the overexpression of a keratinase gene in Bacillus subtilis to enhance the degradation of chicken feathers. The recombinant strain showed increased activity of disulfide reductase and keratinase, resulting in the rapid degradation of feathers and accumulation of free amino acids. Structural characterizations confirmed the breakdown of feather microstructure and polypeptide bonds by the recombinant strain. This study provides new insights into the mechanism of keratin degradation and offers a potential recombinant strain for the valorization of keratin wastes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Dairy & Animal Science
Kai-Lin Xu, Guo-Xin Gong, Miao Liu, Lu Yang, Ze-Jing Xu, Si Gao, Meng-Yi Xiao, Tao Ren, Bing-Ji Zhao, Mahmoud M. Khalil, Ling Zhao, Lv-Hui Sun
Summary: This study found that dietary supplementation of keratinase at 200,000 U/kg can improve the performance, meat quality, apparent total tract digestibility of nutrients, and redox status of broiler chickens fed a diet containing feather meal.