Journal
FEMS MICROBIOLOGY LETTERS
Volume 300, Issue 1, Pages 90-96Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01770.x
Keywords
microcin; oxygen consumption; antibiotic; antimicrobial peptide; respiratory chain; superoxide
Categories
Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [PICT 2107, PAE 22642, PICTO 843]
- CONICET
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Escherichia coli microcin J25 (MccJ25) is a lasso-peptide antibiotic comprising 21 l-amino acid residues (G1-G-A-G-H5-V-P-E-Y-F10-V-G-I-G-T15-P-I-S-F-Y20-G). MccJ25 has two independent substrates: RNA-polymerase (RNAP) and the membrane respiratory chain. The latter is mediated by oxygen consumption inhibition together with an increase of superoxide production. In the present paper, the antibiotic MccJ25 was engineered by substituting Tyr9 or Tyr20 with phenylalanine. Both mutants were well transported into the cells and remained active on RNAP. Only the Y9F mutant lost the ability to overproduce superoxide and inhibit oxygen consumption. The last results confirm that the Tyr9, and not Tyr20, is involved in the MccJ25 action on the respiratory chain target.
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