Journal
FEMS MICROBIOLOGY LETTERS
Volume 292, Issue 2, Pages 231-239Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01487.x
Keywords
Dyella ginsengisoli; 2; 3-dihydroxybiphenyl 1; 2-dioxygenase; kinetic parameters; enzyme-substrate complex
Categories
Funding
- National Natural Science Foundation of China [50608011]
Ask authors/readers for more resources
A bphC gene (915 bp) encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC) was amplified by PCR from Dyella ginsengisoli LA-4, which was heterologously expressed in Escherichia coli. The purified His-Tag BphC was able to catalyze the meta-cleavage reaction of the dihydroxylated aromatic rings. According to the specificity constant (K-cat/K-m) of BphC_LA-4, the specificity of BphC_LA-4 was determined in the following order: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-chlorocatechol > 4-methylcatechol. The experimental data were consistent with the prediction of enzyme-substrate complexes. The highest specific activity of BphC_LA-4 was 118.3 U mg(-1) for 2,3-dihydroxybiphenyl.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available