Review
Biochemistry & Molecular Biology
Adrien Biessy, Martin Filion
Summary: Plant-beneficial Pseudomonas spp. aggressively colonize the rhizosphere and produce secondary metabolites like DAPG, which has broad-spectrum antimicrobial activity and contributes to disease suppression. This review discusses the biosynthesis, regulation, and role of phloroglucinol derivatives in Pseudomonas, including their mode of action as antibiotics, signaling molecules, and potential pathogenicity factors. The genetic and genomic diversity of DAPG-producing Pseudomonas spp. and its importance for enhancing biocontrol of plant pathogens are also explored.
Article
Biochemistry & Molecular Biology
Manthos Panou, Spyros Gkelis
Summary: This study isolated and characterized 81 strains of cyanobacteria from different environments, resulting in the description of five new genera and four new species. The widespread production of HCN among different taxa and habitats was reported for the first time, showcasing novel cyanobacteria diversity.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Optics
Georg Ch Mellau, Vladimir Yu Makhnev, Iouli E. Gordon, Nikolay F. Zobov, Jonathan Tennyson, Oleg L. Polyansky
Summary: A newly developed hydrogen cyanide line list with higher accuracy and completeness for the HITRAN spectroscopic database is presented. By combining experimental results and new variational calculation methods, accurate and complete line lists can be obtained. Through validation against experimental results, the accuracy of the line list is confirmed.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Chemistry, Organic
Caroline Grundke, Caleb Kong, Christopher J. Kampf, B. Frank Gupton, D. Tyler McQuade, Till Opatz
Summary: An efficient, inexpensive, and reliable synthesis of diaminomaleonitrile (DAMN) starting from acetone cyanohydrin as the source of hydrogen cyanide (HCN) is described. The formation mechanism of DAMN through organosulfur catalysis was investigated using electrospray ionization mass spectrometry (ESI-MS) with newly synthesized cationic marker molecules, confirming the proposed thiocyanate-mediated synthesis of the HCN tetramer DAMN starting from organic disulfides.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Environmental Sciences
Tiange Li, Zelong Wang, Bin Yuan, Chenshuo Ye, Yi Lin, Sihang Wang, Qing'e Sha, Zibing Yuan, Junyu Zheng, Min Shao
Summary: The study found that emission factors of carboxylic acids are significantly higher in diesel vehicles compared to gasoline vehicles, with formic acid being a major component. The emission factors of toxic species HCN and HNCO were also quantified. Primary emissions from vehicles were shown to contribute significantly to organic acids in urban environments.
ATMOSPHERIC ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Nicholas J. Green, David A. Russell, Sasha H. Tanner, John D. Sutherland
Summary: Amino acids and their derivatives play a crucial role in the transition from prebiotic chemistry to early biology. This study focuses on the formation and reactions of aminonitriles and their formylated derivatives in formamide. The findings suggest that N-formylaminonitriles can be formed from aldehydes and cyanide in formamide, even without added ammonia, providing a potential prebiotic source of amino acid derivatives. The alkaline processing of N-formylaminonitriles results in a selective hydration at the nitrile group, protecting the aminonitrile derivatives and yielding mixtures of N-formylated and unformylated amino acid derivatives. Additionally, the synthesis of N-formyldehydroalanine nitrile from glycolaldehyde and cyanide in formamide is observed, highlighting its potential role as a precursor for prebiotic peptide synthesis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Plant Sciences
Tingting Ji, Si Ma, Meiting Liang, Xueyun Wang, Lihong Gao, Yongqiang Tian
Summary: This study identified the optimal reference genes for cucumber under M. incognita stress and Pseudomonas treatment, providing potential insights into the interaction between M. incognita or Pseudomonas soil rhizosphere microbe and cucumber.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Perumal Suresh, Murukesan Rekha, Subramanian Gomathinayagam, Vellaisamy Ramamoorthy, Mahaveer P. Sharma, Perumal Sakthivel, Karuppannan Sekar, Mariadhas Valan Arasu, Vellasamy Shanmugaiah
Summary: Microbial bio-products, such as 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas fluorescens, have potential as an alternative to chemical pesticides for managing crop diseases. In this study, the inhibitory efficacy of DAPG against Ralstonia solanacearum was assessed. The study also revealed the beneficial characteristics of Pseudomonas fluorescens, such as the production of antimicrobial substances and the ability to solubilize phosphate. Additionally, the application of Pseudomonas fluorescens and DAPG reduced bacterial wilt incidence and promoted the growth of tomato seedlings.
Article
Forestry
Xenia Hao-Yi Yeoh, Blessing Durodola, Kathrin Blumenstein, Eeva Terhonen
Summary: The major threats to sustainable supply of forest tree products are adverse climate, pests, and diseases. Norway spruce is highly vulnerable to climate change, with pathogens potentially benefiting from under drought stress. Research shows that water availability disturbances can negatively impact tree hosts and enhance pathogen infection ability, especially under drought conditions.
Article
Plant Sciences
Mohsin Nawaz, Liao Li, Farrukh Azeem, Samina Shabbir, Ali Zohaib, Umair Ashraf, Hubiao Yang, Zhiyong Wang
Summary: In this study, differentially expressed genes (DEGs) in Axonopus compressus under drought stress were identified and compared with the control group. The findings suggest the presence of a strong mechanism of drought tolerance in Axonopus compressus, providing a framework for further investigations into the specific roles of these genes in response to drought.
Article
Chemistry, Analytical
Zhongqing Liu, Hongzhou He, Yangui Chen, Jieqing Zheng, Huanghuang Zhuang
Summary: This study uses computational fluid dynamics to investigate the distribution of carbon monoxide (CO) and hydrogen cyanide (HCN) released from the combustion of flexible polyurethane foam (FPUF) in fires. Experimental results determine the yields of CO and HCN under different ventilation conditions, and a pyrolysis and combustion model of FPUF is constructed to simulate the reactions. The study finds that the yields of CO and HCN increase with limited ventilation, particularly in under-ventilated conditions.
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
(2023)
Article
Fisheries
Xiao-wen Wang, Shu-sheng Xiao, Rong Zhang, Lili Liu, Hua Zhu
Summary: Acute hypoxia had significant effects on the physiological responses and mRNA expression of Hypoxia-inducible factors (HIFs) in Siberian sturgeon. The study revealed changes in serum biochemical parameters, immune related enzyme activity, and the expression levels of hif-alpha genes in response to hypoxia stress. Sturgeons exhibited enhanced antioxidant capacity and upregulation of Abhif-1 alpha and Abhif-3 alpha transcription in heart, gill, and brain under acute hypoxia exposure.
Article
Microbiology
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu
Summary: This study tested the stability of ten classic housekeeping genes under the pressure of eight commonly used antibiotics in the laboratory. Results showed that the stability of gene expression was indeed affected by the types of antibiotics added, and the best reference gene set varied for different antibiotics. This study provides a comprehensive summary of the effects of laboratory antibiotics on the stability of housekeeping genes in P. aeruginosa, highlighting the necessity to select housekeeping genes according to the type of antibiotics used in the initial stage of experiment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Perumal Suresh, Govintharaj Varathraju, Vellasamy Shanmugaiah, Khalid S. Almaary, Yahya B. Elbadawi, Ayman Mubarak
Summary: The study demonstrated the antimicrobial potential of partially purified DAPG extracted from Pseudomonas fluorescens VSMKU3054 against Ralstonia solanacearum and fungal pathogens. The isolated strain and DAPG showed significant control against wilt disease of tomato, especially in inhibiting the growth of R. solanacearum. These findings suggest that P. fluorescens VSMKU3054 and DAPG could be utilized as a potential bioinoculant for managing bacterial wilt disease in tomato plants.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Microbiology
Sylvie Letoffe, Yongzheng Wu, Sophie E. Darch, Christophe Beloin, Marvin Whiteley, Lhousseine Touqui, Jean-Marc Ghigo
Summary: This study demonstrates that hydrogen cyanide (HCN) released by Pseudomonas aeruginosa inhibits the growth of Staphylococcus aureus, particularly in low-oxygen environments. These findings are significant for understanding infectious diseases like cystic fibrosis in polymicrobial communities.
Article
Biotechnology & Applied Microbiology
J. A. Jimenez, A. Novinscak, M. Filion
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Plant Sciences
Tanya Arseneault, Roxane Roquigny, Amy Novinscak, Claudia Goyer, Martin Filion
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2020)
Article
Microbiology
Dominique Comeau, Amy Novinscak, David L. Joly, Martin Filion
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Plant Sciences
Cindy Hudec, Amy Novinscak, Martin Filion
Summary: This study characterized the genetic and phenotypic diversity of pathogenic Streptomyces spp. causing Common scab (CS) in Prince Edward Island, Canada. Analysis revealed 14 distinct genetic groups, with varying levels of virulence. The most virulent genetic groups seemed to dominate the pathogenic Streptomyces spp. populations in each field, highlighting the importance of understanding population dynamics for managing this potato disease efficiently.
Article
Microbiology
Jesus A. Jimenez, Amy Novinscak, Martin Filion
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Plant Sciences
Carole Balthazar, Gabrielle Cantin, Amy Novinscak, David L. Joly, Martin Filion
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biodiversity Conservation
Tony Yang, Newton Lupwayi, St-Arnaud Marc, Kadambot H. M. Siddique, Luke D. Bainard
Summary: Anthropogenic interventions have significant impacts on soil biology and health, including promoting crop diversification, agronomic practices, and fertilizer use which may have positive or negative effects on soil. Establishing a rational balance is crucial for maintaining agroecosystem productivity and soil health.
GLOBAL ECOLOGY AND CONSERVATION
(2021)
Review
Biochemistry & Molecular Biology
Adrien Biessy, Martin Filion
Summary: Plant-beneficial Pseudomonas spp. aggressively colonize the rhizosphere and produce secondary metabolites like DAPG, which has broad-spectrum antimicrobial activity and contributes to disease suppression. This review discusses the biosynthesis, regulation, and role of phloroglucinol derivatives in Pseudomonas, including their mode of action as antibiotics, signaling molecules, and potential pathogenicity factors. The genetic and genomic diversity of DAPG-producing Pseudomonas spp. and its importance for enhancing biocontrol of plant pathogens are also explored.
Article
Plant Sciences
Fahad Alotaibi, Soon-Jae Lee, Marc St-Arnaud, Mohamed Hijri
Summary: In this study, bacteria were isolated from the rhizosphere and surrounding soil of Salix purpurea and Eleocharis obusta from a former petrochemical plant site in Canada. A total of 438 morphologically different bacterial isolates were obtained, with approximately 32% capable of utilizing all five different hydrocarbons compounds. Furthermore, 5% of tested isolates possessed all five of the tested plant growth-promoting functional traits.
Article
Microbiology
Antoine Zboralski, Adrien Biessy, Martin Filion
Summary: The distribution and functions of Type III secretion systems (T3SSs) in two important bacterial groups, nitrogen-fixing rhizobia and plant-beneficial Pseudomonas spp., are discussed. T3SSs and their effectors play important roles in legume-rhizobia symbiosis and in plant-beneficial Pseudomonas spp. The diversity of T3SSs in plant-beneficial bacteria suggests their significant roles in interkingdom interactions in the rhizosphere.
Article
Microbiology
Andrew J. C. Blakney, Luke D. Bainard, Marc St-Arnaud, Mohamed Hijri
Summary: Soil history plays a crucial role in shaping the structure and biodiversity of soil bacterial communities. This study evaluated the impact of different soil histories on the bacterial communities associated with five Brassicaceae host plants. The results showed that the Brassicaceae hosts consistently influenced the bacterial community structure, while the previous year's soil history only had a significant effect when the host plant feedback was weakened. Understanding soil history is essential for utilizing microbiome technologies to improve agricultural systems.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Andrew J. C. Blakney, Luke D. Bainard, Marc St-Arnaud, Mohamed Hijri
Summary: Oomycetes play a critical role in soil microbial communities and have significant impacts on agriculture. However, they are understudied compared to bacteria and fungi, and our understanding of their biodiversity and community structure in soil over time is limited. This study investigated the influence of soil history on Oomycetes communities in the roots and rhizosphere of different Brassicaceae crops. The results showed that soil history and chemistry had a significant impact on the structure and biodiversity of Oomycetes communities, highlighting the importance of agricultural practices in shaping future microbial communities.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Microbiology
Martin Filion
Article
Food Science & Technology
Amy Novinscak, Martin Filion
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2020)
Review
Biochemistry & Molecular Biology
Antoine Zboralski, Martin Filion
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2020)
