Article
Immunology
Rosanna Leuzzi, Margherita Bodini, Isaac P. Thomsen, Elisabetta Soldaini, Erika Bartolini, Alessandro Muzzi, Bruna Clemente, Bruno Galletti, Andrea Guido Oreste Manetti, Cinzia Giovani, Stefano Censini, Sonia Budroni, Fabiana Spensieri, Erica Borgogni, Silvia Rossi Paccani, Immaculada Margarit, Fabio Bagnoli, Giuseppe Del Giudice, Clarence B. Creech
Summary: The study identified specific cytokine and functional antibody signatures in patients with different primary invasive diseases caused by Staphylococcus aureus. These data provide insights into human responses to invasive staphylococcal infections and are important for guiding the identification of novel preventive and therapeutic interventions against S. aureus. Our findings showed distinct differences in inflammatory responses and antibody levels between patients with invasive S. aureus disease and healthy donors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Tomasz K. Prajsnar, Justyna J. Serba, Bernice M. Dekker, Josie F. Gibson, Samrah Masud, Angeleen Fleming, Simon A. Johnston, Stephen A. Renshaw, Annemarie H. Meijer
Summary: The study reveals that intracellular autophagy in neutrophils may have both beneficial and detrimental effects on hosts infected with Staphylococcus aureus, with different pathways leading to conflicting outcomes.
Article
Microbiology
Paroma Roy, Alexander R. Horswill, Paul D. Fey
Summary: The adherence of S. epidermidis to corneocytes is dependent on the accumulation-associated protein Aap, which interacts through the A and B domains. Adherence to corneocytes relies on the lectin subdomain within the A domain, with the A repeat domain playing a key role in Aap stability.
Article
Immunology
Elena Boero, Iris Brinkman, Thessely Juliet, Eline van Yperen, Jos A. G. van Strijp, Suzan H. M. Rooijakkers, Kok P. M. van Kessel
Summary: Neutrophils play a crucial role in the immune response to Staphylococcus aureus infections by rapidly migrating to the infection site to engulf and destroy bacteria. A high-throughput flow cytometry assay was described to quantify phagocytosis of S. aureus by human neutrophils, showing that opsonization greatly enhances bacterial uptake. This rapid assay can be used to study neutrophil deficiencies and bacterial evasion, as well as to assess the opsonic capacity of antibodies in natural immune responses or therapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Manuel Salzmann, Harald Platzer, Marion Mussbacher, Martina Derler, Max Lenz, Patrick Haider, Mira Brekalo, Julia B. Kral-Pointner, Stefan Kastl, Walter S. Speidl, Klaus T. Preissner, Uwe Schubert, Markus Bischoff, Pavel Uhrin, Johann Wojta, Philipp J. Hohensinner
Summary: Atherosclerosis is a chronic, inflammatory disease of the vessel wall characterized by the interaction between immune cells and inflamed endothelium. Extracellular adherence protein (Eap) from Staphylococcus aureus can inhibit the interaction between leukocytes and activated endothelial cells. In a study using ApoE-deficient mice on a high-fat diet, Eap treatment reduced platelet-neutrophil aggregates, T cell and neutrophil infiltration, and neutrophil extracellular trap formation in developing atherosclerosis. However, Eap did not significantly change plaque size. In established plaques, Eap intervention did not cause cellular or morphological changes, but increased T cell infiltration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Microbiology
Ming-Shan Chen, Yi-Wei Tung, Chia-Lin Hu, Hui-Ju Chang, Wen-Chun Lin, Shew-Meei Sheu
Summary: The study found that three different lipid emulsions reduced reactive oxygen species production and bacterial phagocytosis, leading to increased bacterial survival.
Article
Biochemistry & Molecular Biology
Kimberly J. Dunham-Snary, Bas G. J. Surewaard, Jeffrey D. Mewburn, Rachel E. T. Bentley, Ashley Y. Martin, Oliver Jones, Ruaa Al-Qazazi, Patricia A. D. Lima, Paul Kubes, Stephen L. Archer
Summary: Mitochondria in human neutrophils play a critical role in the killing of Staphylococcus aureus. They contribute to bacterial clearance through mechanisms such as phagocytosis and vital NETosis.
Article
Dermatology
Ching Yang, Juan de Dios Ruiz-Rosado, Frank H. Robledo-Avila, Zhaotao Li, Ryan N. Jennings, Santiago Partida-Sanchez, Christopher P. Montgomery
Summary: The study demonstrates the protective effect of antibodies against alpha-hemolysin in skin and soft tissue infection. Antibodies lead to robust neutrophilic inflammation and compartmentalization of bacteria into discrete abscesses, reducing dermonecrosis and enhancing bacterial clearance.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Microbiology
Marleen Leidecker, Anne Bertling, Muzaffar Hussain, Markus Bischoff, Johannes A. Eble, Anke C. Fender, Kerstin Jurk, Christine Rumpf, Mathias Herrmann, Beate E. Kehrel, Silke Niemann
Summary: Invasion of host cells by Staphylococcus aureus is facilitated by fibronectin bridges and phagocytosis. The secreted extracellular adherence protein (Eap) promotes this cellular uptake pathway, including bacteria poorly taken up by host cells. The role of protein disulfide isomerase (PDI) in this process is highlighted.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Seo-Young Kim, Minjun Kim, Tae-Jong Kim
Summary: This study reveals a mechanism for regulating biofilm formation in Staphylococcus aureus through strain-specific physiological changes induced by a plant steroid called diosgenin. Diosgenin was found to either reduce or increase biofilm formation in different strains of S. aureus by affecting the expression of regulatory genes associated with physiological changes. The study provides insights into the signal transduction mechanisms that regulate biofilm formation in S. aureus based on physiological and gene expression changes.
Article
Immunology
Zhaotao Li, Pavani Beesetty, George Gerges, Maureen Kleinhenz, Melissa Moore-Clingenpeel, Ching Yang, Luul B. Ahmed, Josey Hensley, Lisa Steele, Anita S. Chong, Christopher P. Montgomery
Summary: The study found that S. aureus-specific antibody levels and T-cell responses increase with age, indicating a coordinated development of anti-staphylococcal immunity. Young infected children had higher levels of certain antibodies compared to healthy children, but these differences disappeared with age. Both global and S. aureus-specific T-cell function were impaired in children with invasive and noninvasive infections.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biology
Mara D. Saenz-de-Juano, Giulia Silvestrelli, Andres Weber, Christian Rohrig, Mathias Schmelcher, Susanne E. Ulbrich
Summary: This study investigates the impact of Staphylococcus aureus extracellular vesicles on mammary epithelial cells, providing insights into how the bacteria evades host defense mechanisms and colonizes the mammary gland effectively.
Article
Immunology
Kristina D. D. Hinman, Sonia S. S. Laforce-Nesbitt, Joshua T. T. Cohen, Miles Mundy, Joseph M. M. Bliss, Alexander R. R. Horswill, Craig T. T. Lefort
Summary: Techniques for studying bacterial infection clearance are critical for understanding diseases, immune cell functions, and antimicrobial therapeutics. This study develops a bi-fluorescent assay to monitor Staphylococcus aureus killing by neutrophils in vivo. The use of a stable secondary fluorescent marker allows for the separation of infected cell samples and evaluation of neutrophil function in a murine model of chronic granulomatous disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Pietro Speziale, Giampiero Pietrocola
Summary: NETs are essential components of the innate immune system during bacterial infections. Staphylococcus aureus can evade NET-mediated killing through various mechanisms, such as degrading NETs and inhibiting their formation. Understanding the virulence factors involved in NET formation/neutralization could lead to potential therapeutic interventions against this pathogen.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Food Science & Technology
Xiaogang Wang, Paul F. Koffi, Olivia F. English, Jean C. Lee
Summary: Staphylococcus aureus generates and releases extracellular vesicles (EVs) containing toxins and other virulence factors that can affect host cells, with production influenced by various environmental stressors. Temperature and culture conditions have an impact on the production of EVs by S. aureus.
Article
Cell Biology
Mohamed Ibrahem Elhawy, Sylvaine Huc-Brandt, Linda Patzold, Laila Gannoun-Zaki, Ahmed Mohamed Mostafa Abdrabou, Markus Bischoff, Virginie Molle
Summary: Staphylococcus aureus remains a significant public health threat, especially in hospital settings. Studying the molecular and cellular mechanisms involved in pathogenesis, host adaptation, and virulence is crucial for developing effective treatment strategies. The modulation of phosphatases-mediated regulation plays a critical role in various host-pathogen interactions, including oxidative stress adaptation during infection.
Article
Biochemistry & Molecular Biology
Divyendu Goud Thalla, Philipp Jung, Markus Bischoff, Franziska Lautenschlaeger
Summary: Extracellular vimentin plays a role in inflammation regulation, cell migration, and cancer cell proliferation. It enhances cell permeability, but this effect is altered by the SARS-CoV-2 receptor binding domain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Philipp Jung, Xiangda Zhou, Sandra Iden, Markus Bischoff, Bin Qu
Summary: The study found that T cells are stiffer in the lamellipodia than in the cell body when forming immunological synapse. Additionally, upon formation of the immunological synapse, T cell stiffness is enhanced both at the lamellipodia and on the cell body.
Article
Microbiology
Linda Paetzold, Alexandra Stark, Felix Ritzmann, Carola Meier, Thomas Tschernig, Joerg Reichrath, Robert Bals, Markus Bischoff, Christoph Beisswenger
Summary: The IL-17C/IL-17RE axis plays a role in wound closure in infected wounds, but does not affect the elimination of S. aureus.
Article
Biochemistry & Molecular Biology
Christian Spengler, Friederike Nolle, Nicolas Thewes, Ben Wieland, Philipp Jung, Markus Bischoff, Karin Jacobs
Summary: This study investigates the adhesion of Staphylococcus aureus to abiotic surfaces of different wettability through numerous single-cell force spectroscopy measurements. The results reveal that the pathogen utilizes different cell wall molecules and interaction mechanisms when binding to hydrophobic and hydrophilic surfaces. Additionally, the interplay of electrostatic effects of charges and protein composition on bacterial surfaces plays a predominant role on hydrophilic surfaces, while the influence of the high number of binding proteins is highlighted on hydrophobic surfaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ahmad Aljohmani, Bastian Opitz, Markus Bischoff, Daniela Yildiz
Summary: This study found that infection with Pseudomonas aeruginosa and Streptococcus pneumoniae stimulates the activation of ADAM10 in epithelial cells, leading to inflammatory cell recruitment and loss of barrier integrity. The activation is based on the toxin repertoire rather than the bacterial particle itself.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Walter Hofer, Emilia Oueis, Antoine Abou Fayad, Felix Deschner, Anastasia Andreas, Lais Pessanha de Carvalho, Stephan Huettel, Steffen Bernecker, Linda Paetzold, Bernd Morgenstern, Nestor Zaburannyi, Markus Bischoff, Marc Stadler, Jana Held, Jennifer Herrmann, Rolf Mueller
Summary: The rise of antimicrobial resistance poses a severe threat to public health. Chlorotonil, a new antibiotic targeting multidrug resistant pathogens, was found to have promising activities. However, its pharmaceutical properties needed improvement. By semisynthesis, derivatives with enhanced solubility were obtained, and their stability and activity were verified in vivo.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Hannah Benthien, Beate Fresenborg, Linda Paetzold, Mohamed Ibrahem Elhawy, Sylvaine Huc-Brandt, Christoph Beisswenger, Gabriela Krasteva-Christ, Soren L. Becker, Virginie Molle, Johannes K. Knobloch, Markus Bischoff
Summary: This study reveals the essential role of SpoVG in the biofilm formation of Staphylococcus epidermidis on artificial surfaces. SpoVG promotes biofilm formation by modulating the expression of ica genes. However, SpoVG is largely dispensable for biofilm formation of this skin commensal under in vivo conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minh-Thu Nguyen, Leonhard Hubert Schellerhoff, Silke Niemann, Frieder Schaumburg, Mathias Herrmann
Summary: This article introduces an improved protocol for preparing monocytes, which allows the assessment of cytokine release upon external stimulation. By adding a quiescence step, the protocol provides a stable and activatable pure monocyte population.
Review
Microbiology
Chantal Akoua-Koffi, Adele Kacou N'Douba, Joseph Allico Djaman, Mathias Herrmann, Frieder Schaumburg, Silke Niemann
Summary: The Staphylococcus aureus-related complex is composed of multiple species, with S. schweitzeri mainly found in African wildlife and rarely causing infections in humans. However, misidentification may lead to underreporting of S. schweitzeri in humans. Despite the low number of isolates in humans, in vitro assays suggest that S. schweitzeri might be as virulent as S. aureus. Antimicrobial resistance studies show that S. schweitzeri is susceptible to tested antibiotics.
Review
Biology
Esther Olunoiki, Jacqueline Rehner, Markus Bischoff, Elena Koshel, Thomas Vogt, Jorg Reichrath, Soren L. Becker
Summary: This article provides an overview of the relationship between skin microbiota and health, as well as the association between various skin diseases and microbial dysbiosis. It also discusses how recent research approaches in skin microbiome studies may lead to improved diagnostic and therapeutic methods in the foreseeable future.
Article
Microbiology
Charlotte Huber, Silver A. Wolf, Wilma Ziebuhr, Mark A. Holmes, Julia Assmann, Antina Luebke-Becker, Andrea Thuermer, Torsten Semmler, Julian Brombach, Astrid Bethe, Markus Bischoff, Lothar H. Wieler, Lennard Epping, Birgit Walther
Summary: Previous research found limited differences between MSSA and MRSA isolated from pig farming environments, mainly related to the absence of SCCmec and some single nucleotide polymorphisms. LA-MRSA were shown to discard replicative SCCmec elements through homologous recombination to save energy from an evolutionary perspective. MRSA exhibited differential global transcriptomic responses when exposed to specific pig-farm associated conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Ahmed Mohamed Mostafa Abdrabou, Issa Sy, Markus Bischoff, Manuel J. Arroyo, Soeren L. Becker, Alexander Mellmann, Lutz von Mueller, Barbara Gaertner, Fabian K. Berger
Summary: This study evaluated the feasibility of using MALDI-TOF technology combined with machine learning models to distinguish between hypervirulent and non-hypervirulent strains of Clostridioides difficile. The results showed that certain machine learning models could accurately differentiate between hypervirulent and non-hypervirulent strains with a high accuracy rate and further sub-cluster the hypervirulent strains into three subgroups. The conclusion is that MALDI-TOF combined with machine learning is a reliable tool for rapid identification of major European hypervirulent strains.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
Nadhuma Youssouf, Marianne Martin, Markus Bischoff, Philippe Soubeyran, Laila Gannoun-Zaki, Virginie Molle
Summary: Staphylococcus aureus inhibits the host SUMOylation response to enhance its survival and persistence within the host.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Monika Hollenhorst, Rajender Nandigama, Saskia B. Evers, Igor Gamayun, Noran Abdel Wadood, Alaa Salah, Mario Pieper, Amanda Wyatt, Alexey Stukalov, Anna Gebhardt, Wiebke Nadolni, Wera Burow, Christian Herr, Christoph Beisswenger, Soumya Kusumakshi, Fabien Ectors, Tatjana Kichko, Lisa Huebner, Peter Reeh, Antje Munder, Sandra-Maria Wienhold, Martin Witzenrath, Robert Bals, Veit Flockerzi, Thomas Gudermann, Markus Bischoff, Peter Lipp, Susanna Zierler, Vladimir Chubanov, Andreas Pichlmair, Peter Koenig, Ulrich Boehm, Gabriela Krasteva-Christ
Summary: This study reveals that tracheal brush cells express functional taste receptors and that bitter taste signaling induces neurogenic inflammation, which mediates secretion of immune mediators and defense against bacterial infections.
JOURNAL OF CLINICAL INVESTIGATION
(2022)