Article
Immunology
Justin S. Bae, Fei Da, Ryan Liu, Lei He, Huiying Lv, Emilie L. Fisher, Govindarajan Rajagopalan, Min Li, Gordon Y. C. Cheung, Michael Otto
Summary: The study found that Staphylococcal enterotoxin B (SEB) contributes significantly to the pathogenicity of Staphylococcus aureus, suggesting the value of including SEB as a target in multipronged antistaphylococcal drug development strategies. Furthermore, it indicates that SEB contributes to fatal exacerbation of community-associated methicillin-resistant S. aureus infection.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Shakilur Rahman, Kasturi Sarkar, Amit Kumar Das
Summary: In this study, an in-silico approach was used to design a stable, safe and immunogenic vaccine against Staphylococcus aureus. Three important pathogenic toxins of S. aureus were selected and their epitopes were predicted and combined to form a multi-epitope vaccine. The final vaccine was found to be antigenic and non-allergenic, and showed potential immune-stimulating properties along with cloning feasibility.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Multidisciplinary Sciences
Juliana Karla Garcia Ribeiro Freitas, Cristiane Fernandes de Assis, Thailla Raquel Moura de Oliveira, Claudio Marcio de Medeiros Maia, Bruno Jonatan de Sousa, Gidyenne Christiane Bandeira Silva de Medeiros, Larissa Mont'Alverne Juca Seabra, Karla Suzanne Florentino da Silva Chaves Damasceno
Summary: This study describes a systematic review protocol with meta-analysis on the prevalence and types of staphylococcal enterotoxins in food, and the profile of contaminated foods.
Article
Biotechnology & Applied Microbiology
Rohini Krishna Kota, Harish Babu Kolla, Prakash Narayana Reddy, Naveen Kumar Kalagatur, Siva Kumar Samudrala
Summary: A novel multi-antigen subunit vaccine was designed, incorporating three protective antigens and validated through immunoinformatics analysis and computational approaches. The recombinant HAB protein (r-HAB) showed high immunogenicity in mice, inducing strong antibody responses and excellent protection against native toxins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Respiratory System
Ha-Kyeong Won, Youngsang Yoo, Jiwon Lee, Noeul Kang, Ji-Hyang Lee, Jun-Pyo Choi, Tae-Bum Kim, Sang-Heon Cho, Woo-Jung Song
Summary: This prospective observational study investigates whether Staphylococcus aureus nasal colonization and staphylococcal enterotoxin-specific IgE sensitization synergistically influence the clinical outcomes of adults with late-onset asthma. The study suggests that SA nasal colonization and SE-IgE sensitization may have a synergistic effect on disease severity in late-onset asthmatics.
Article
Microbiology
Ulrica Thunberg, Svante Hugosson, Ralf Ehricht, Stefan Monecke, Elke Mueller, Yang Cao, Marc Stegger, Bo Soderquist
Summary: This study analyzed Staphylococcus aureus diversity, genetic factors, and humoral immune responses in chronic rhinosinusitis (CRS) patients through long-term follow-up. The results showed that some patients retained S. aureus isolates from the same genetic lineage over a decade, but the overall changes in antibody responses to staphylococcal antigens were highly variable, with no correlation found between antigen-encoding genes and corresponding antibody levels in serum.
Article
Biotechnology & Applied Microbiology
L. Schwendimann, D. Merda, T. Berger, S. Denayer, C. Feraudet Tarisse, A. J. Klaui, S. Messio, M. Y. Mistou, Y. Nia, J. A. Hennekinne, H. U. Graber
Summary: This study investigated the relationship between the vSa beta type, clonal complex (CC), and the production of enterotoxins SEG and SEI by Staphylococcus aureus strains. It was found that the amount of SEG and SEI produced correlated with the vSa beta type and CC of a strain, and the whole-genome sequencing data could be used to predict enterotoxin production and source of an outbreak strain. These results show promising indications for better understanding the role SEG and SEI play in foodborne outbreaks caused by S. aureus.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Cell Biology
Zahra Chegini, Mojtaba Didehdar, Amin Khoshbayan, Jafar Karami, Milad Yousefimashouf, Aref Shariati
Summary: Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a multifactorial disease, and bacterial superantigens such as Staphylococcus aureus are known to play a role in its development. Specifically, Staphylococcus aureus nasal colonization and IgE response to Staphylococcus aureus enterotoxins have been found in CRSwNP patients. Among the enterotoxins, enterotoxin B (SEB) has been identified as a key player in the pathogenesis of CRSwNP by disrupting mucosal integrity and triggering inflammatory responses. SEB activates Toll Like Receptor 2, leading to the production of pro-inflammatory cytokines, and induces cellular stress and oxidative damage. It also promotes eosinophil degranulation and localized eosinophilic inflammation. Furthermore, SEB may be involved in the regulation of immune cells and maintenance of sinonasal mucosal inflammation. While in vitro and animal studies have provided valuable insights, further research is needed to fully understand the role of SEB in CRSwNP.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Chemistry, Analytical
Beibei Lu, Jialei Bai, Jingyang Zhang, Huijing Shen, Minglin Wang, Yujing Lian, Zhixian Gao, Yuan Peng
Summary: A novel iridescent polymeric film is reported, which can respond to different concentrations of Staphylococcus aureus enterotoxin B (SEB) in the visible range. The film is fabricated with a polymerization process using methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA), and gold nanoparticles (AuNPs) are incorporated into the polymer structure. This film exhibits a LOD of 4.29 fg mL-1 for SEB, making it the best performing colorimetric material to date.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Immunology
Fengqing Hou, Lianci Peng, Jiali Jiang, Tingting Chen, Dongyi Xu, Qingyuan Huang, Chao Ye, Yuanyi Peng, Dong-Liang Hu, Rendong Fang
Summary: Staphylococcus aureus is an important zoonotic food-borne pathogen that can cause severe infections. A study found that the enterotoxin O of S. aureus can induce interleukin-1 beta secretion in mouse neutrophils, which is dependent on the activation of TLR4/JNK/NLRP3 inflammasome signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Dermatology
Osamu Yamasaki, Satoru Sugihara, Ai Kajita, Emi Yokoyama, Tomoko Miyake, Yoji Hirai, Shin Morizane
Summary: Toxic shock syndrome (TSS) is caused by toxins produced by Staphylococcus aureus, with lipopolysaccharide (LPS) also playing a significant role in sepsis development. Staphylococcal superantigens and LPS work together to induce cytokine release and lethal shock.
JOURNAL OF DERMATOLOGY
(2021)
Article
Microbiology
Yuko Shimamura, Rina Noaki, Yukino Oura, Kenya Ichikawa, Toshiyuki Kan, Shuichi Masuda
Summary: Patients with diabetes are more prone to Staphylococcus aureus infections. The study evaluated the impact of staphylococcal enterotoxin A (SEA) on immune responses of diabetic mice spleen cells and the effects of polyphenols on inflammation-related gene expression. (-)-Epigallocatechin gallate (EGCG) interacted with SEA, while nobiletin did not. Exposure to SEA enhanced the expression of certain genes in spleen cells of diabetic mice, and both EGCG and nobiletin showed potential in inhibiting inflammation by affecting gene expression.
Article
Food Science & Technology
Junyan Liu, Tengyi Huang, Thanapop Soteyome, Yuzhu Mao, Yuting Luo, Lei Yuan, Qin Ma, Gongliang Liu, Chunyun Qu, Gamini Seneviratne, Birthe Kjellerup, Zhenbo Xu
Summary: Food safety issues caused by Staphylococcus aureus contamination during low-temperature food storage have been studied. The results showed that S. aureus did not enter the VBNC state during low temperature storage and the enterotoxin gene seb was continuously expressed in most samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Immunology
Jin Yuan, Ping Tong, Xuanyi Meng, Yong Wu, Xin Li, Jinyan Gao, Hongbing Chen
Summary: This study investigated the effects of co-exposure to Staphylococcus aureus enterotoxin B (SEB) and Ovalbumin (OVA) on the development of food allergy. The results suggested that this co-exposure could impair the intestinal barrier, disrupt the intestinal Th immune balance, and decrease the diversity of intestinal flora in OVA-sensitized mice. Additionally, it enhanced the transport of OVA in the Caco-2 cell monolayer, promoted the uptake and presentation of OVA in bone marrow dendritic cells (BMDCs), and enhanced Th cell differentiation. These findings suggest that SEB in allergens should be considered a risk factor for food allergy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Patrick M. Schlievert
Summary: In this study, vaccine toxoids of staphylococcal enterotoxins B and C were prepared by site-specific mutagenesis, resulting in the loss of biological toxicity but the ability to vaccinate rabbits against lethal toxic shock syndrome.
MICROBIOLOGY SPECTRUM
(2023)
Article
Respiratory System
Yves Gillet, Anne Tristan, Jean-Philippe Rasigade, Mitra Saadatian-Elahi, Coralie Bouchiat, Michele Bes, Oana Dumitrescu, Marie Leloire, Celine Dupieux, Frederic Laurent, Gerard Lina, Jerome Etienne, Philippe Vanhems, Laurent Argaud, Francois Vandenesch
Summary: This study found that severe pneumonia caused by Staphylococcus aureus was associated with PVL toxin in toddlers and was a risk factor for mortality in older patients. Additionally, methicillin resistance and other virulence factors were also associated with mortality.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Review
Microbiology
Mariane Pivard, Karen Moreau, Francois Vandenesch
Summary: Staphylococcus aureus, a bacterium that can be part of the normal human flora or cause infections, has a wide range of virulence factors that contribute to its ability to cause severe infections, including community-acquired pneumonia. These virulence factors help S. aureus survive in the lower respiratory tract, cross the pulmonary epithelial barrier, evade host defenses, and ultimately lead to severe tissue damage and adverse clinical outcomes. Researchers continue to study these factors and their implications for future research.
Article
Microbiology
Charlotte Genestet, Elisabeth Hodille, Alexia Barbry, Jean-Luc Berland, Jonathan Hoffmann, Emilie Westeel, Fabiola Bastian, Michel Guichardant, Samuel Venner, Gerard Lina, Christophe Ginevra, Florence Ader, Sylvain Goutelle, Oana Dumitrescu
Summary: The study highlights the importance of identifying specific sub-populations of Mycobacterium tuberculosis that may tolerate anti-TB drugs, potentially leading to treatment failure. Identifying these sub-populations could help improve personalized TB management and prevent the emergence of drug resistance in TB patients.
Article
Infectious Diseases
Tiphaine Gaillard, Celine Dupieux-Chabert, Marine Butin, Oana Dumitrescu, Oilida Naceur, Caroline Bouveyron, Annie Martra, Michele Bes, Anne Tristan, Francois Vandenesch, Gerard Lina, Frederic Laurent, Jean-Philippe Rasigade
Summary: Comparing the development of vancomycin resistance under in vitro exposure, it was found that VSSA strains develop vancomycin resistance faster than hVISA strains.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Emma Desgranges, Laura Barrientos, Lucas Herrgott, Stefano Marzi, Alejandro Toledo-Arana, Karen Moreau, Francois Vandenesch, Pascale Romby, Isabelle Caldelari
Summary: The study focused on the sRNA RsaG derived from Staphylococcus aureus and its induction with uhpT when bacteria are internalized into host cells or in the presence of mucus-secreting cells. RsaG was found to control redox homeostasis and adjust metabolism by stabilizing, degrading, or repressing the translation of mRNA targets.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Elisabeth Hodille, Laetitia Beraud, Severine Perian, Valentine Berti, Michele Bes, Anne Tristan, Emilie Blond, Gerard Lina, Oana Dumitrescu
Summary: Staphylococcus aureus produces virulence factors that are affected differently by subinhibitory concentrations of antibiotics, with oxacillin inhibiting the production of PSM alpha 1 and Hld, while the effects of other antibiotics vary depending on the strain or clone.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Andrea Cara, Tristan Ferry, Frederic Laurent, Jerome Josse
Summary: Gram-negative bacilli can cause prosthetic joint infection, and the treatment becomes challenging due to increased antibiotic resistance and biofilm formation. Loading bone cement with antibiotics can help reduce the risk of infection during arthroplasties with cemented prosthesis. The study compared the efficacy of various antibiotic-loaded bone cements in preventing biofilm formation by different strains of Gram-negative bacteria.
Article
Microbiology
Vincent Le Moigne, Anne-Laure Roux, Helene Mahoudo, Gaetan Christien, Agnes Ferroni, Oana Dumitrescu, Gerard Lina, Jean-Philippe Bouchara, Patrick Plesiat, Jean-Louis Gaillard, Stephane Canaan, Genevieve Hery-Arnaud, Jean-Louis Herrmann
Summary: IgA serological assay is found to be clinically useful in the diagnosis of NTM infections, particularly Mycobacterium abscessus, in patients with cystic fibrosis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Charlotte Genestet, Elisabeth Hodille, Albin Bernard, Maxime Vallee, Gerard Lina, Adrien Le Meur, Guislaine Refregier, Oana Dumitrescua
Summary: This study compared membrane-based spoligotyping with whole-genome sequencing (WGS)-based spoligotyping and found good overall agreement between the two methods. In silico spoligotyping could be used as a smooth transition for tuberculosis (TB) diagnosis and epidemiological survey.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
R. A. N. C. Anne-Gaelle, S. E. N. T. I. S. Celia, C. O. U. T. U. R. I. E. R. Jeanne, B. A. R. B. U. T. Frederic, T. R. I. S. T. A. N. Anne, B. U. I. S. Coralie, S. A. N. T. O. S. Kevin, F. A. R. R. A. H. Sonia, D. U. R. A. F. F. O. U. R. G. Pascaline, V. A. N. D. E. N. E. S. C. H. Francois, D. A. U. W. A. L. D. E. R. Olivier, L. A. U. R. E. N. T. Frederic
Summary: Toxigenic Clostridioides difficile infection is a growing concern in both hospital and community settings. The prevalence of toxigenic C. difficile was estimated to be 5.3% using the routine algorithm and 7.4% using the BDM screening method. The overall agreement between BDM and the routine algorithm was 97.4%, which increased to 99.4% after resolving discrepancies through additional testing.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Alexandre Bourles, Anne Tristan, Francois Vandenesch, Michele Bes, Frederic Laurent, Anne-Gaelle Ranc, Malia Kainiu, Ann-Claire Gourinat, Antoine Biron, Cecile Cazarola, Cyrille Goarant, Julien Colot
Summary: This study investigated the epidemiology of MRSA in New Caledonia and identified a low diversity with the dominance of a clonal complex not reported previously. The recommendation to stop the topical use of fusidic acid was made to control the emergence of this severe MRSA clone.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Medicine, General & Internal
Vasileios Giovanoulis, Angelo V. Vasiliadis, Christos Koutserimpas, George Samonis, Cecile Batailler, Tristan Ferry, Sebastien Lustig
Summary: This study reports an extremely rare fungal infection caused by Candida lusitaniae after revised total knee replacement. The patient was successfully treated with multiple strategies, highlighting the importance of a multidisciplinary approach for managing severe infections.
Article
Infectious Diseases
Benjamin Youenou, Patricia Martins Simoes, Anne Tristan, Eric Farfour, Clemence Beauruelle, Camille Kolenda, Anne-Gaelle Ranc, Francois Vandenesch, Frederic Laurent, Celine Dupieux
Summary: This study describes the presence of linezolid-resistant MRSA strains carrying the cfr(B) gene in the French National Reference Centre for staphylococci. The strains showed resistance to linezolid but susceptibility to tedizolid, and carried the cfr(B) gene on the same chromosomal transposon. This finding highlights the risk of horizontal transfer of oxazolidinone resistance genes in Staphylococcus aureus.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Ines Rodrigues Lopes, Laura Maria Alcantara, Ricardo Jorge Silva, Jerome Josse, Elena Pedrero Vega, Ana Marina Cabrerizo, Melanie Bonhomme, Daniel Lopez, Frederic Laurent, Francois Vandenesch, Miguel Mano, Ana Eulalio
Summary: This study investigates the interaction of 191 clinical isolates of Staphylococcus aureus with host cells, revealing the prevalence of intracellular replication and persistence. The study highlights the impact of phenotypic variation on infection characteristics and suggests potential implications for the treatment of staphylococcal infections.
NATURE COMMUNICATIONS
(2022)
Article
Food Science & Technology
Marie Courcon, Cedric Badiou, Mathilde Louwagie, Sibyle Etievant, Michel Jaquinod, Gerard Lina, Virginie Brun
Summary: This study presents a mass-spectrometry-based proteomics workflow for the analysis of Staphylococcus aureus superantigenic toxins in menstrual fluid. The toxins were detected in varying concentrations in samples from patients with mTSS and healthy women.