Article
Genetics & Heredity
Jie Wei, Huan Zhang, Xiaoya Ma, Yujie Li, Wenqian Zhou, Jinping Guo, Tianbo Jin, Mingjun Hu
Summary: This study investigated the association between single nucleotide polymorphisms (SNPs) of the OR51E1 gene and glioma susceptibility in the Chinese Han population. The results showed that polymorphisms rs10768148, rs7102992, and rs10500608 were associated with glioma risk in the overall sample. Stratified analyses based on gender and age revealed specific SNP-glioma associations. Additionally, synergistic and redundant relationships between certain SNPs were identified. Overall, this study provides a basis for assessing glioma risk-associated variants in the Chinese Han population.
Article
Infectious Diseases
Felipe Dominguez Machado, Mirela Gehlen, Vitoria Schmidt Caron, Gabriel Tassi Mousquer, Graziele Lima Bello, Camila Anton, Rafaela Manzoni Bernardi, Alana Ambos Freitas, Gisela Unis, Elis Regina Dalla Costa, Maria Lucia Rosa Rossetti, Denise Rossato Silva
Summary: The genotype 5/5 and allele 5 of MIF-794 CATT (5-8) were more common among TB patients compared to controls. There was no difference in the prevalence of genotypes 7/X+8/X between cases and controls.
Article
Biophysics
Wei-Ting Chen, Ping-Yeh Chiu, Chien-Fu Chen
Summary: In recent years, there has been an increasing demand for rapid, sensitive, and simple methods for diagnosing DNA due to the rise in infectious disease variations. This study introduces a flash signal amplification method combined with electrochemical detection for PCR-free tuberculosis molecular diagnosis. By utilizing the properties of butanol and water, we were able to concentrate the necessary components for DNA detection quickly, reducing reaction time. The developed method shows high sensitivity and specificity, capable of detecting low levels of DNA targets and is successfully applied in tuberculosis diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Genetics & Heredity
Li-Rong Yan, Zhi Lv, Jing-Jing Jing, Yuan Yuan, Qian Xu
Summary: Through meta-analysis, it was found that SNPs related to immune response are closely associated with AG risk, suggesting their potential as predictive biomarkers for AG. Further updated meta-analyses based on extensive relevant studies are needed for more reliable results.
Review
Oncology
Guanwu Wang, Lara Rosaline Heij, Dong Liu, Edgar Dahl, Sven Arke Lang, Tom Florian Ulmer, Tom Luedde, Ulf Peter Neumann, Jan Bednarsch
Summary: This systematic review evaluated the association between single-nucleotide polymorphisms (SNPs) and cholangiocarcinoma (CCA), focusing on tumorigenesis and prognosis. Based on 34 studies, a total of 43 SNPs in 32 genes associated with CCA risk, metastatic progression, and overall prognosis were identified. The study comprehensively described the mechanisms and potential clinical implications associated with these SNPs. The findings suggest that multiple SNPs play different roles at different stages of CCA and may serve as biomarkers for guiding treatment and assessing oncological risk.
Article
Biochemistry & Molecular Biology
Hanyi Zhang, Siyao Deng, Jiayu Zhang, Guiquan Zhu, Jie Zhou, Wenjing Ye, Qingwei Wang, Yi Wang, Bingwen Zou, Peng Zhang, Shichuan Zhang, Jinyi Lang, Shun Lu
Summary: Genetic variants in genes involved in the LMP1 signaling pathway, particularly the NFKBIA promoter SNP rs2233409, may be moderately associated with the risk of nasopharyngeal carcinoma (NPC). Further studies with larger sample sizes and functional analysis are needed to confirm these findings.
Article
Oncology
Zheng Lv, Changgui Kou, Naifei Chen, Lin Jia, Xu Sun, Yangyang Gao, Rilan Bai, Ming Yang, Jiuwei Cui
Summary: This study indicates that HOTAIR SNPs (rs920778 and rs4759314) are significantly associated with BC susceptibility and prognosis in the northeastern Chinese population, highlighting their importance in the occurrence and development of BC.
FRONTIERS IN ONCOLOGY
(2021)
Article
Infectious Diseases
Meng-Rui Lee, Yen-Lin Chen, Chang-Wei Wu, Lun-Che Chen, Lih-Yu Chang, Jung-Yueh Chen, Yu-Tsung Huang, Jann-Yuan Wang, Jin-Yuan Shih, Chong-Jen Yu
Summary: This study found that single nucleotide polymorphisms (SNPs) in Toll-like receptor 1 (TLR1), Toll-like receptor 2 (TLR2), and matrix metalloproteinase 8 (MMP8) were associated with the risk of tuberculosis (TB) infection and disease. Haplotypes and a polygenic risk score (PRS) could be used to predict TB disease status.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Shuyuan Liu, Nannan Liu, Hui Wang, Xinwen Zhang, Yufeng Yao, Shuqiong Zhang, Li Shi
Summary: The study found an association between CCR5 promoter polymorphisms and pulmonary TB and TB progression in the Chinese Han population.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Wenjing Zhou, Lijuan Wu, Jiajia Song, Lin Jiao, Yi Zhou, Juan Zhou, Nian Wang, Tangyuheng Liu, Zhenzhen Zhao, Hao Bai, Tao Wu, Binwu Ying
Summary: The study found that rs10841856 in the CLEC4E gene may be associated with an increased risk of tuberculosis in male Han individuals from Western China.
BMC INFECTIOUS DISEASES
(2021)
Article
Genetics & Heredity
Baoping Hu, Yuhe Wang, Zhongtao Wang, Xue He, Li Wang, Dongya Yuan, Yongjun He, Tianbo Jin, Shumei He
Summary: By studying the northwest Chinese Han population, this research identified the relationships between polymorphisms in or near the SLC11A1 gene and age- and sex-specific tuberculosis risk.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Ping Fan, Zhiyi Zhang, Linzhi Lu, Xingcai Guo, Zhicheng Hao, Xinghua Wang, Yancheng Ye
Summary: In this study, 31 SNPs were found to be significantly associated with the incidence of GC in Wuwei, Gansu, China. The genotype AC/AA of rs4823921 was significantly associated with poor prognosis of GC patients in Wuwei.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Noha A. Hassuna, Mohamed El Feky, Aliae A. R. Mohamed Hussein, Manal A. Mahmoud, Naglaa K. Idriss, Sayed F. Abdelwahab, Maggie A. Ibrahim
Summary: This study evaluated the role of IL-18 and IFN-γ polymorphisms in susceptibility to tuberculosis infection among Egyptian patients. Results showed a protective effect against TB with IL-18 -137G/C CC genotype, a higher risk with GG and GC genotypes, and a significant risk with INF-γ+874 AA genotype.
Review
Medicine, General & Internal
Juan M. Cubillos-Angulo, Catarina D. Fernandes, Davi N. Araujo, Cristinna A. Carmo, Maria B. Arriaga, Bruno B. Andrade
Summary: Genetic studies have shown the relevance of CD14 and NOD2 gene polymorphisms in association with the risk of diseases caused by Mycobacterium tuberculosis infection. Specific CD14 polymorphisms were found to either increase or decrease the risk of TB disease, while certain NOD2 SNPs were significantly associated with higher risk in the Chinese population. Ethnicity may play a role in the impact of these gene polymorphisms on the risk of Mtb diseases.
SYSTEMATIC REVIEWS
(2021)
Article
Public, Environmental & Occupational Health
Liwei Cheng, Fan Zhang, Ying Wang, Jing Chen, Xiaoping Yuan
Summary: This meta-analysis study found an association between IFNGR1 gene polymorphisms and tuberculosis susceptibility. The rs2234711 polymorphism was associated with increased tuberculosis susceptibility in Africans and decreased susceptibility in Asians. Additionally, the rs7749390 polymorphism was associated with decreased tuberculosis susceptibility in Africans.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Fermin Acosta, Anders Norman, Dilcia Sambrano, Victoria Batista, Igor Mokrousov, Egor Shitikov, Julio Jurado, Maritza Mayrena, Odemaris Luque, Maybis Garay, Laura Solis, Patricia Munoz, Dorte B. Folkvardsen, Troels Lillebaek, Laura Perez-Lago, Amador Goodridge, Dario Garcia de Viedma
Summary: This study identified a specific Beijing strain in the Panamanian province of Colon, showing increased virulence and resistance, likely introduced from Vietnam between 2000 and 2012. The strain is predominant in the province, but has low number of cases caused by recent transmission.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Igor Mokrousov
Summary: The Euro-American lineage of Mycobacterium tuberculosis, comprising genetically and geographically diverse families, shows that spoligotype SIT53 occupies a basal position in evolution but does not correspond to any specific family. Different SIT53 subpopulations have evolved into various distant families through long-term allopatric evolution.
Article
Microbiology
Igor Mokrousov, Oksana Pasechnik, Anna Vyazovaya, Irina Yarusova, Alena Gerasimova, Aleksey Blokh, Viacheslav Zhuravlev
Summary: This study found that infection with different subtypes of the Beijing genotype can impact the progression and lethal outcome of pulmonary tuberculosis. Patients infected with the highly lethal Beijing 14717-15 subtype had the highest mortality rate, while those infected with the low-virulence 1071-32 subtype may lead to chronic TB. Therefore, in the treatment of TB, strain virulence should be considered in addition to drug resistance.
Article
Multidisciplinary Sciences
Igor Mokrousov, Anna Vyazovaya, Viacheslav Sinkov, Alena Gerasimova, Panayotis Ioannidis, Weiwei Jiao, Polina Khromova, Dimitrios Papaventsis, Oksana Pasechnik, Joao Perdigao, Nalin Rastogi, Adong Shen, Yuriy Skiba, Natalia Solovieva, Philip Suffys, Silva Tafaj, Tatiana Umpeleva, Diana Vakhrusheva, Irina Yarusova, Svetlana Zhdanova, Viacheslav Zhuravlev, Oleg Ogarkov
Summary: An ancient sublineage of the Mycobacterium tuberculosis Beijing genotype, endemic to East Asia, has been found to include a novel clonal group called Beijing 1071-32, which is exclusively multi/extensive drug resistant. A real-time PCR assay has been developed for the detection of this cluster, showing 100% specificity and sensitivity in genetically diverse M. tuberculosis isolates collected from various regions. This study highlights the epidemiological significance of the Beijing 1071-32 cluster and the utility of the PCR assay for tracking this MDR strain.
SCIENTIFIC REPORTS
(2021)
Article
Veterinary Sciences
Violeta Valcheva, Claudia Perea, Tanya Savova-Lalkovska, Albena Dimitrova, Lukasz Radulski, Igor Mokrousov, Krustyu Marinov, Hristo Najdenski, Magdalena Bonovska
Summary: The recent Mycobacterium bovis/M. caprae isolates from Bulgaria showed significant molecular diversity, with most isolates exhibiting a high level of genetic divergence. Limited active transmission of bTB in Bulgaria during the survey period was suggested by the phylogenetic analysis, while the possibility of endemic presence of circulating bTB strains could not be completely ruled out.
BMC VETERINARY RESEARCH
(2022)
Article
Infectious Diseases
Svetlana Zhdanova, Igor Mokrousov, Elizaveta Orlova, Viacheslav Sinkov, Oleg Ogarkov
Summary: Eastern Siberia and Mongolia are high-incidence regions for tuberculosis in Asia. This study found significant differences in the transmission of Mycobacterium tuberculosis between the two countries, with a higher rate of drug resistance in Russia. However, the transborder transmission of tuberculosis between Mongolia and its neighbors has been negligible, and the drug resistance trends in Russia did not impact the current situation in Mongolia.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Ivaylo M. Slavchev, Yavor Mitrev, Boris Shivachev, Violeta Valcheva, Marine Dogonadze, Natalia Solovieva, Anna Vyazovaya, Igor Mokrousov, Wolfgang Link, Lucia Jimenez, Bastien Cautain, Thomas A. Mackenzie, Isabel Portugal, Francisca Lopes, Rita Capela, Joao Perdigao, Georgi M. Dobrikov
Summary: This study describes the synthesis of arylidenecamphors and pyrimidines with camphane skeleton, some of which exhibit excellent antimicrobial activity against various strains of pathogenic bacteria and fungi. These newly designed compounds also demonstrate in vitro cytotoxicity.
Article
Multidisciplinary Sciences
Zofia Bakula, Valentine B. I. Wuyep, Lukasz Bartocha, Anna Vyazovaya, Eugene I. Ikeh, Jacek Bielecki, Igor Mokrousov, Tomasz Jagielski
Summary: Nigeria has the highest burden of tuberculosis in Africa and ranks 6th globally. However, few studies have examined the molecular epidemiology of Mycobacterium tuberculosis in the country. This study analyzed the genetic structure of drug-resistant tuberculosis in central Nigeria and found that the Cameroon and T lineages were the most prevalent, with no association between the Cameroon lineage and drug resistance.
Article
Chemistry, Medicinal
Igor Mokrousov, Ivaylo Slavchev, Natalia Solovieva, Marine Dogonadze, Anna Vyazovaya, Violeta Valcheva, Aleksey Masharsky, Olesya Belopolskaya, Simeon Dimitrov, Viacheslav Zhuravlev, Isabel Portugal, Joao Perdigio, Georgi M. Dobrikov
Summary: New nitrofuranyl amides were synthesized and their anti-TB activity and genetic response of mycobacteria were investigated. The study demonstrated the potential resistance mechanisms mediated by certain genes and their roles in oxidative and nitrosative stress responses.
Article
Microbiology
Anna Vyazovaya, Alena Gerasimova, Regina Mudarisova, Daria Terentieva, Natalia Solovieva, Viacheslav Zhuravlev, Igor Mokrousov
Summary: The Beijing genotype of Mycobacterium tuberculosis is the predominant family in Northwestern Russia, with a high proportion of multidrug-resistant strains. Central Asian/Russian and B0/W148 sublineages were identified, and the latter exhibited a higher frequency of MDR compared to the former. Various profiles and clusters were observed in the Beijing strains, with some types showing a high prevalence of MDR and others consisting only of drug-susceptible strains. The transmission of MDR B0/W148 strains is contributing to the increase in MDR-TB cases in the region.
Article
Biochemistry & Molecular Biology
Svetlana Zhdanova, Wei-Wei Jiao, Viacheslav Sinkov, Polina Khromova, Natalia Solovieva, Alexander Mushkin, Igor Mokrousov, Olesya Belopolskaya, Aleksey Masharsky, Anna Vyazovaya, Lubov Rychkova, Lubov Kolesnikova, Viacheslav Zhuravlev, A-Dong Shen, Oleg Ogarkov
Summary: This study analyzed the drug resistance patterns of Mycobacterium tuberculosis strains from children with tuberculosis in China and Russia. It found that the Beijing genotype was dominant in both countries, with the MDR strains being more prevalent in the Russian collection. The study also revealed differences in resistance mutations between Chinese and Russian rifampicin-resistant strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Irina Kostyukova, Oksana Pasechnik, Igor Mokrousov
Summary: In this study, the epidemiological situation and drug resistance patterns of Mycobacterium tuberculosis in the Omsk region of Russia were investigated. The TB incidence decreased over time, but the rate of multidrug-resistant tuberculosis (MDR-TB) increased. Drug resistance was found in more than half of the isolates tested, with a significant proportion of MDR cases. Monitoring drug resistance is crucial due to the emergence of M. tuberculosis strains with resistance to multiple drugs.
Article
Medicine, General & Internal
Daria Terentieva, Tanya Savova-Lalkovska, Albena Dimitrova, Magdalena Bonovska, Igor Mokrousov, Violeta Valcheva
Summary: This study aimed to assess the diversity of minisatellite VNTR loci in Mycobacterium bovis/M. caprae isolates in Bulgaria and their position within global M. bovis diversity. A total of 43 M. bovis/M. caprae isolates from cattle in Bulgaria collected between 2015 and 2021 were typed in 13 VNTR loci. The results showed clear separation of M. bovis and M. caprae branches on the VNTR phylogenetic tree. The larger and more geographically dispersed M. caprae group exhibited higher diversity compared to the M. bovis group (HGI 0.67 vs. 0.60). Six clusters and nine orphans were identified, with locus QUB3232 being the most discriminatory (HGI 0.64). Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between M. bovis and M. caprae. Comparison with VNTR datasets from 11 countries showed overall heterogeneity between settings and predominantly local evolution of clonal complexes. In conclusion, six loci (ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 with HGI 0.77) are recommended for primary genotyping of M. bovis/M. caprae isolates in Bulgaria. VNTR typing based on a limited number of loci appears to be useful for primary bovine tuberculosis surveillance.
Article
Biochemistry & Molecular Biology
A. A. Vyazovaya, N. Yu Gavrilova, A. A. Gerasimova, A. O. Bychkova, I Avadenii, E. Anikieva, N. S. Solovieva, V. Yu Zhuravlev, I. Mokrousov, O. Narvskaya
Summary: This study characterized the molecular-genetic profile of Mycobacterium tuberculosis strains in Murmansk oblast and found that the Beijing genotype was the dominant strain with a high proportion of multiple drug resistance. Over the past 15 years, there has been an increase in the prevalence of the Beijing genotype in the region.
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2022)
Meeting Abstract
Respiratory System
Igor Mokrousov, Anna Vyazovaya, Svetlana Zhdanova, Tatiana Umpeleva, Maria Badleeva, Irina Yarusova, Alena Gerasimova, Regina Mudarisova, Natalia Solovieva, Natalia Eremeeva, Diana Vakhrusheva, Oksana Pasechnik, Oleg Ogarkov, Viacheslav Zhuravlev, Tatiana Umpeleva
EUROPEAN RESPIRATORY JOURNAL
(2021)