Journal
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
Volume 57, Issue 2, Pages 193-201Publisher
WILEY
DOI: 10.1111/j.1574-695X.2009.00599.x
Keywords
antimethicillin-resistant Staphylococcus aureus; cytoplasmic leakage; Eleutherine americana; membrane morphology
Categories
Funding
- Prince of Songkla University Graduate Fund
- Thailand Research Fund: Basic Research [DBG 5180021]
Ask authors/readers for more resources
The anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity and the possible mechanism of action of a crude extract from red bulbs of Eleutherine americana Merr. were investigated. The crude ethanolic extract from E. americana produced minimum inhibitory concentrations (MICs) of 62.5-1000 and 250 mu g mL-1 against MRSA isolates and the reference strains, respectively. Treatment of S. aureus ATCC 27664 with a crude extract at 2MIC reduced the inoculum size by 5 log at 24 h compared with the control. The combined effect of the extract and 7.5% NaCl on the enterotoxin-producing ATCC strain resulted in no detection of organisms within 24 h compared with the control. The release of cell materials after extract treatment was determined by measuring OD260 nm, the treatment resulted in cytoplasmic leakage. Determination of OD620 nm showed that the extract did not cause gross cell wall damage. However, observation of S. aureus cells under an electron microscope after treatment with 2MIC and 4MIC of the crude extract revealed that the extract caused damage to membrane morphology. A knowledge of the mechanism of action of the E. americana extract may offer useful hints in the search for novel antibacterial substance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available