Journal
FEBS LETTERS
Volume 588, Issue 19, Pages 3520-3525Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.05.040
Keywords
Molecular motors; Single-molecule imaging; Sub-diffraction localization; Kinesin; Dynein; Myosin; Processivity; Intracellular transport; Cytoskeletal motors; Motility; Total internal reflection fluorescence microscopy; TIRF
Funding
- NIH [GM094522]
- NSF CAREER [MCB-1055017]
- NSF Graduate Research Fellowship [DGE 1106400]
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Processive cytoskeletal motors from the myosin, kinesin, and dynein families walk on actin filaments and microtubules to drive cellular transport and organization in eukaryotic cells. These remarkable molecular machines are able to take hundreds of successive steps at speeds of up to several microns per second, allowing them to effectively move vesicles and organelles throughout the cytoplasm. Here, we focus on single-molecule fluorescence techniques and discuss their wide-ranging applications to the field of cytoskeletal motor research. We cover both traditional fluorescence and sub-diffraction imaging of motors, providing examples of how fluorescence data can be used to measure biophysical parameters of motors such as coordination, stepping mechanism, gating, and processivity. We also outline some remaining challenges in the field and suggest future directions. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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